Table B.1. Comparisons of Tryptic Digest of CYP2E1 from Different Sources

Fig B.1. 1D SDS-PAGE of recombinant CYPs expressed in E. coli (Xenotech/Cypex). Each CYP (labeled on the top) is loaded and run at two concentrations, where the first lane is 10 pmol and the second is 20 pmol with respect to CYPs.

Table B.1. Comparisons of tryptic digest of CYP2E1 from different sources.

Start-end / Sequence / Mr / Recombinant CYP (Panvera) * / Recombinant CYP in bactosomes / Human microsomes
(% experiments found) / Comments
64-76 / FGPVFTLYVGSQR / 1469.77 / + / + / 69
77-84 / MVVMHGYK / 963.43 / – / – / 9
88-100 / EALLDYKDEFSGR / 1541.74 / + / + / 88 / missed cleavage
101-110 / GDLPAFHAHR / 1119.56 / + / + / 88
113-123 / GIIFNNGPTWK / 1245.65 / + / – / 59
128-134 / FSLTTLR / 836.48 / + / – / –
150-159 / EAHFLLEALR / 1197.65 / + / + / 94
161-186 / TQGQPFDPTFLIGCAPCNVIADILFR / 2950.46 / – / – / 25 / carbamidated
188-198 / HFDYNDEKFLR / 1483.70 / + / – / 78 / missed cleavage
252-263 / EHHQSLDPNCPR / 1489.66 / + / + / 59 / carbamidated
264-275 / DLTDCLLVEMEK / 1465.69 / – / – / 9
283-309 / LYTMDGITVTVADLFFAGTETTSTTLR / 2924.46 / – / + / 31
310-317 / YGLLILMK / 950.57 / – / – / 41
318-324 / YPEIEEK / 907.44 / – / – / 38
325-331 / LHEEIDR / 911.46 / + / + / 44
345-359 / QEMPYMDAVVHEIQR / 1844.86 / + / + / 16
360-374 / FITLVPSNLPHEATR / 1693.92 / + / + / 94
386-408 / GTVVVPTLDSVLYDNQEFPDPEK / 2562.26 / – / + / 88
409-420 / FKPEHFLNENGK / 1458.73 / + / + / 28 / missed cleavage
423-434 / YSDYFKPFSTGK / 1438.68 / + / + / 59
436-444 / VCAGEGLAR / 932.46 / + / – / 22 / carbamidated
468-484 / DIDLSPIHIGFGCIPPR / 1906.98 / – / + / 63
4685-492 / YKLCVIPR / 1048.57 / + / – / – / missed cleavage, carbamidated

* Supplied as a purified protein

“–“ means no corresponding tryptic peptide detected

Table B.2. Comparisons of tryptic digest of CYP2C19 from different sources.

Start-end / Sequence / Mr / Recombinant CYP (Panvera) * / Recombinant CYP in bactosomes / Human microsomes
(% experiments found) / Comments
60-73 / IYGPVFTLYFGLER / 1673.88 / + / + / 25
74-84 / MVVLHGYEVVK / 1272.69 / – / + / –
98-105 / GHFPLAER / 925.48 / + / – / –
109-119 / GFGIVFSNGKR / 1180.64 / + / – / – / missed cleavage
161-185 / ASPCDPTFILGCAPCNVICSIIFQK / 2868.36 / – / + / 25
207-232 / IVSTPWIQICNNFPTIIDYFPGTHNK / 3075.54 / – / + / 25
250-261 / EHQESMDINNPR / 1468.64 / + / + / 25
276-307 / QNQQSEFTIENLVITAADLLGAGTETTSTTLR / 3422.73 / – / + / 50
343-357 / GHMPYTDAVVHEVQR / 1737.83 / + / + / 25
358-375 / YIDLIPTSLPHAVTCDVK / 1984.03 / + / – / –
384-399 / GTTILTSLTSVLHDNK / 1698.92 / – / + / –
400-410 / EFPNPEMFDPR / 1377.60 / + / + / –
422-432 / SNYFMPFSAGK / 1247.56 / + / – / –

* Supplied as a purified protein

“–“ means no corresponding tryptic peptide detected