Table 7 ANOVA of the central composite design

S / SS and MS / DF / F value / Prob>F
Cms / Fps / Bgl / Cms / Fps / Bgl / Cms / Fps / Bgl
A / 619.3 / 167.3 / 0.7 / 1 / 4.6 / 6.0 / 6.4 / 0.0 / 0.0 / 0.0
B / 1131.4 / 7.3 / 2.2 / 1 / 8.5 / 0.3 / 0.0 / 0.0 / 0.6 / 0.9
C / 111.7 / 7.3 / 2.2 / 1 / 0.8 / 0.3 / 20.8 / 0.4 / 0.6 / 0.0
D / 2665.4 / 1091.4 / 0.7 / 1 / 19.9 / 39.1 / 6.4 / 0.0 / 0.0 / 0.0
A2 / 2583.5 / 476.5 / 1.6 / 1 / 19.3 / 17.1 / 15.0 / 0.0 / 0.0 / 0.0
B2 / 26.3 / 4.2 / 0.0 / 1 / 0.2 / 0.2 / 0.3 / 0.7 / 0.7 / 0.6
C2 / 1.2 / 0.8 / 0.0 / 1 / 9.2 / 0.0 / 0.2 / 0.9 / 0.9 / 0.7
D2 / 0.8 / 0.0 / 0.1 / 1 / 6.1 / 7.9 / 1.1 / 0.9 / 1.0 / 0.3
AB / 71.3 / 0.2 / 0.0 / 1 / 0.5 / 6.8 / 0.4 / 0.5 / 0.9 / 0.5
AC / 11.0 / 1.8 / 5.5 / 1 / 0.1 / 0.1 / 0.1 / 0.8 / 0.8 / 0.8
AD / 311.0 / 0.4 / 0.0 / 1 / 2.3 / 0.0 / 0.2 / 0.1 / 0.9 / 0.7
BC / 17.8 / 1.5 / 0.1 / 1 / 0.1 / 0.1 / 1.4 / 0.7 / 0.8 / 0.3
BD / 94.5 / 0.1 / 9.5 / 1 / 0.7 / 4.0 / 9.0 / 0.4 / 1.0 / 0.9
CD / 37.7 / 2.3 / 0.1 / 1 / 0.3 / 0.1 / 0.9 / 0.6 / 0.8 / 0.4
S / SS / DF / MS / F value / Prob>F
Cms / Fps / Bgl / Cms / Fps / Bgl / Cms / Fps / Bgl / Cms / Fps / Bgl
Model / 7750.4 / 1780.4 / 5.6 / 14 / 553.6 / 127.2 / 0.4 / 4.1 / 4.6 / 3.8 / 0.0 / 0.0 / 0.0
Residual / 2007.6 / 418.6 / 1.6 / 15 / 133.8 / 27.9 / 0.1
Lack of Fit / 2007.6 / 418.6 / 1.6 / 10 / 200.8 / 41.9 / 0.2 / - / - / - / - / - / -
Pure error / 0.0 / 0.0 / 0.0 / 5 / 0.0 / 0.0 / 0.0
Cor Total / 9757.9 / 2199.1 / 7.2 / 29

S- source; SS- sum of squares; MS- mean square; DF- degrees of freedom

A- (NH4)2SO4; B- MnCl2.4H2O; C- Glycine; D- Na-CMC

Factor linear effects (A, B, C, D); squared effects (A2,B2, C2, D2); combined effects (AB, AC, AD, BC, BD, CD)

Cms- CMCase; Fps-FPase; Bgl- β-Glucosidase

Values of "Prob > F" less than 0.05 indicate model terms are significant

For CMCase- R-Squared (0.8), Adj R-Squared (0.6), Pred R-Squared (-0.2); forFPase- R-Squared (0.8), Adj R-Squared (0.6), Pred R-Squared (-0.1); forβ-Glucosidase- R-Squared (0.8), Adj R-Squared (0.6), Pred R-Squared (-0.3)