In vitroPGPR trait / Medium/Method / Reference / Positive control / Negative control
Phosphate solubilization / NBRIP / Nautiyal 1999 / EnterobactercloacaeNeua1027 / AzotobacterchroococcumBNMb363
Siderophores / CAS / Schwyn and Neilands 1987 / Pseudomonasfluorescens Neu1043 / A. chroococcum Neu1159
N2 fixation / N-free / Döbereiner et al. 1980 / Azospirillum brasilense245 / EscherichiacoliNeu1006
Aryl-sulphatases / modified Angle / Kertesz and Mirleau 2004ADDIN CSL_CITATION { "citationItems" : [ { "id" : "ITEM-1", "itemData" : { "DOI" : "Doi 10.1007/Bf02539153", "ISBN" : "0095-3628", "abstract" : "The 16S rDNA genes of an apparently pure culture of a psychrophilic and strict barophilic bacterium (WHB 46) were studied by PCR-mediated amplification and cloning into phage M13 mp 18. Sequence analysis of five individual clones revealed the presence of two different 16S rDNA types. The homology value of 90% indicates that culture WHB 46 is actually composed of two closely related species (WHB 46-1 and 46-2). Both strains are members of the gamma-subdivision of proteobacteria. Analysis of a sixth clone (WHB 46-1/2) leads to the conclusion that it represents a 16S rDNA hybrid molecule assembled during the PCR reaction. This hypothesis was confirmed by secondary structure analysis of the chimeric rDNA. The appearance of such hybrid molecules point to a potential risk in studies on the diversity of bacterial populations by analysis of rDNA pattern via PCR-mediated amplification because they suggest the existence of organisms that do not actually exist in the sample investigated.", "author" : [ { "dropping-particle" : "", "family" : "Liesack", "given" : "W", "non-dropping-particle" : "", "parse-names" : false, "suffix" : "" }, { "dropping-particle" : "", "family" : "Weyland", "given" : "H", "non-dropping-particle" : "", "parse-names" : false, "suffix" : "" }, { "dropping-particle" : "", "family" : "Stackebrandt", "given" : "E", "non-dropping-particle" : "", "parse-names" : false, "suffix" : "" } ], "container-title" : "Microbial Ecology", "id" : "ITEM-1", "issue" : "3", "issued" : { "date-parts" : [ [ "1991" ] ] }, "note" : "Fy304\nTimes Cited:227\nCited References Count:23", "page" : "191-198", "title" : "Potential Risks of Gene Amplification by Pcr as Determined by 16s Rdna Analysis of a Mixed-Culture of Strict Barophilic Bacteria", "type" : "article-journal", "volume" : "21" }, "uris" : [ " ] } ], "mendeley" : { "formattedCitation" : "(Liesack et al 1991)", "plainTextFormattedCitation" : "(Liesack et al 1991)", "previouslyFormattedCitation" : "(Liesack et al 1991)" }, "properties" : { "noteIndex" : 0 }, "schema" : " } / un-indentified rhizosphere isolate / Enterobacter aerogenes Neu 1036
IAA-like compounds / Salkowski reagent / Bric et al. 1991
Tarnawski et al. 2006 / A.brasilense 245 / P.fluorescensNeu1043
Hydrogen cyanide (HCN) / Synthetic medium with HCNprecursors / Castric and Castric 1983(Schwyn & Neilands, 1987)Tarnawski et al. 2006 / P.fluorescens Neu1201 / P.fluorescens Neu1027
Fungi/Protist growth inhibition / Dual culture assay in PDA / - / P. fluorescens CHAO / E. coli Neu 1006

Table 1S.In vitroPGPR traits analyzed

a Collection University of Neuchâtel

bCollection University of Buenos Aires