Table 1S. A list of bacteriophage vB_EcoM_FV3 putative genes with predicted functions.

GeneNo. / FV3 ORF / Nearesthomologue / Putativefunction
FV3 ORF001 / Escherichia phage rV5 ORF1 / rIIAprotectorfromprophage-inducedearlylysis
FV3 ORF002 / rV5 ORF2 / SleBcellwallhydrolase
FV3 ORF003 / rV5 ORF3 / hypotheticalprotein
FV3 ORF004 / rV5 ORF4 / MoxRATPase
FV3 ORF005 / rV5 ORF5 / hypothetical protein
FV3 ORF006 / rV5 ORF6 / hypothetical protein
FV3 ORF007 / rV5 ORF7 / hypothetical protein
FV3 ORF008 / rV5 ORF8 / hypothetical protein
FV3 ORF009 / rV5 ORF9 / hypothetical protein
FV3 ORF010 / rV5 ORF10 / alpha1-3fucosyltransferase
FV3 ORF011 / rV5 ORF11 / anti-sigmafactor
FV3 ORF012 / rV5 ORF12 / hypothetical protein
FV3 ORF013 / rV5 ORF14 / hypothetical protein
FV3 ORF014 / rV5 ORF15 / hypothetical protein
FV3 ORF015 / rV5 ORF16 / hypothetical protein
FV3 ORF016 / rV5 ORF17 / hypothetical protein
FV3 ORF017 / rV5 ORF18 / hypothetical protein
FV3 ORF018 / rV5 ORF19 / hypothetical protein
FV3 ORF019 / rV5 ORF20 / hypothetical protein
FV3 ORF020 / rV5 ORF21 / hypothetical protein
FV3 ORF021 / rV5 ORF22 / hypothetical protein
FV3 ORF022 / rV5 ORF24 / hypothetical protein
FV3 ORF023 / Enterobacteria phage JSE ORF EpJSE_00144 / hypothetical protein
FV3 ORF024 / Enterobacteria phage RB49 ORF142w / hypothetical protein
FV3 ORF025 / rV5 ORF25 / hypothetical protein
FV3 ORF026 / rV5 ORF26 / hypothetical protein
FV3 ORF027 / rV5 ORF27 / hypothetical protein
FV3 ORF028 / rV5 ORF28 / tailfiberprotein
FV3 ORF029 / rV5 ORF29 / hypothetical protein
FV3 ORF030 / rV5 ORF30 / tailfiberprotein
FV3 ORF031 / rV5 ORF31 / hypothetical protein
FV3 ORF032 / rV5 ORF32 / tailfiberassemblyprotein
FV3 ORF033 / rV5 ORF33 / tailfiberprotein
FV3 ORF034 / rV5 ORF34 / hypothetical protein
FV3 ORF035 / rV5 ORF35 / hypothetical protein, possible structural*
FV3 ORF036 / rV5 ORF36 / hypothetical protein, possible structural*
FV3 ORF037 / rV5 ORF37 / tailfiberprotein
FV3 ORF038 / rV5 ORF38 / hypothetical protein
FV3 ORF039 / rV5 ORF39 / hypothetical protein
FV3 ORF040 / rV5 ORF40 / hypothetical protein
FV3 ORF041 / rV5 ORF41 / tailfiberprotein
FV3 ORF042 / rV5 ORF42 / hypothetical protein, possible structural*
FV3 ORF043 / rV5 ORF43 / hypothetical protein, possible structural*
FV3 ORF044 / rV5 ORF44 / hypothetical protein, possible structural*
FV3 ORF045 / rV5 ORF45 / hypothetical protein
FV3 ORF046 / rV5 ORF46 / hypothetical protein, possible structural*
FV3 ORF047 / rV5 ORF47 / hypothetical protein, possible structural*
FV3 ORF048 / rV5 ORF48 / hypothetical protein, possible structural*
FV3 ORF049 / rV5 ORF49 / hypothetical protein
FV3 ORF050 / rV5 ORF50 / hypothetical protein
FV3 ORF051 / rV5 ORF51 / hypothetical protein
FV3 ORF052 / rV5 ORF52 / structuralprotein
FV3 ORF053 / rV5 ORF53 / structuralprotein
FV3 ORF054 / rV5 ORF54 / hypothetical protein, possible structural*
FV3 ORF055 / rV5 ORF55 / hypothetical protein, possible structural*
FV3 ORF056 / rV5 ORF56 / hypothetical protein
FV3 ORF057 / rV5 ORF57 / hypothetical protein, possible structural*
FV3 ORF058 / rV5 ORF58 / hypothetical protein
FV3 ORF059 / rV5 ORF60 / majorheadprotein
FV3 ORF060 / rV5 ORF61 / headstabilization/decorationprotein
FV3 ORF061 / rV5 ORF62 / hypothetical protein
FV3 ORF062 / rV5 ORF63 / hypothetical protein
FV3 ORF063 / rV5 ORF64 / hypothetical protein, possible structural*
FV3 ORF064 / rV5 ORF64ABC / terminaselargesubunit
FV3 ORF065 / rV5 ORF66 / hypothetical protein
FV3 ORF066 / rV5 ORF67 / hypothetical protein
FV3 ORF067 / rV5 ORF68 / hypothetical protein
FV3_gt001 / tRNA-ProTGG-anticodon
FV3_gt002 / tRNA-MetCAT-anticodon
FV3_gt003 / tRNA-ThrTGT-anticodon
FV3_gt004 / tRNA-TyrGTA-anticodon
FV3_gt005 / tRNA-ArgTCT-anticodon
FV3 ORF068 / rV5 ORF74 / hypothetical protein
FV3 ORF069 / rV5 ORF75 / hypothetical protein
FV3 ORF070 / rV5 ORF76 / hypothetical protein
FV3 ORF071 / rV5 ORF77 / hypothetical protein
FV3 ORF072 / rV5 ORF79 / transcriptionalregulator
FV3 ORF073 / rV5 ORF80 / phosphoesterase
FV3 ORF074 / rV5 ORF81 / hypothetical protein
FV3 ORF075 / rV5 ORF82 / RNAligase1andtailattachmentprotein
FV3 ORF076 / rV5 ORF83 / hypothetical protein
FV3 ORF077 / rV5 ORF84 / Sir2-likeprotein
FV3 ORF078 / rV5 ORF85 / hypothetical protein
FV3 ORF079 / rV5 ORF86 / hypothetical protein
FV3 ORF080 / rV5 ORF87 / hypothetical protein
FV3 ORF081 / rV5 ORF88 / ATP-dependentDNAligase
FV3 ORF082 / rV5 ORF89 / lysozyme
FV3 ORF083 / Salmonella phage PVP-SE1 ORF12 / HNHendonuclease
FV3 ORF084 / rV5 ORF90 / membraneprotein
FV3 ORF085 / rV5 ORF91 / HNHendonuclease
FV3 ORF086 / rV5 ORF92 / transposase-likeprotein
FV3 ORF087 / rV5 ORF93 / hypothetical protein
FV3 ORF088 / rV5 ORF94 / exodeoxyribonuclease
FV3 ORF089 / rV5 ORF95 / hypothetical protein
FV3 ORF090 / rV5 ORF96 / hypothetical protein
FV3 ORF091 / rV5 ORF97 / endoVIIpackagingandrecombinationendonuclease
FV3 ORF092 / rV5 ORF98 / hypothetical protein
FV3 ORF093 / rV5 ORF99 / hypothetical protein
FV3 ORF094 / rV5 ORF100 / DNAN6-adeninemethyltransferase
FV3 ORF095 / rV5 ORF101 / hypothetical protein
FV3 ORF096 / rV5 ORF102 / hypothetical protein
FV3 ORF097 / rV5 ORF103 / hypothetical protein
FV3 ORF098 / rV5 ORF104 / hypothetical protein
FV3 ORF099 / rV5 ORF105 / hypothetical protein
FV3 ORF100 / rV5 ORF106 / thymidylatesynthase
FV3 ORF101 / rV5 ORF107 / hypothetical protein
FV3 ORF102 / rV5 ORF108 / hypothetical protein
FV3 ORF103 / rV5 ORF109 / ribonucleosidetriphosphatereductasealphachain
FV3 ORF104 / rV5 ORF110 / ribonucleoside triphosphate reductase betachain
FV3 ORF105 / rV5 ORF111 / glutaredoxin1
FV3 ORF106 / rV5 ORF112 / anaerobicribonucleoside-triphosphatereductase
FV3 ORF107 / Bacillus sp. BT1B_CT2 ORF names: HMPREF1012_00978 / HNHhomingendonuclease
FV3 ORF108 / rV5 ORF112 / anaerobicribonucleoside-triphosphatereductase
FV3 ORF109 / rV5 ORF117 / anaerobicNTPreductasesmallsubunit
FV3 ORF110 / rV5 ORF118 / hypothetical protein
FV3 ORF111 / rV5 ORF119 / hypothetical protein
FV3 ORF112 / rV5 ORF120 / PhoH-likeprotein
FV3 ORF113 / rV5 ORF121 / hypothetical protein
FV3 ORF114 / rV5 ORF122 / ClpPATP-dependentproteasesubunit
FV3 ORF115 / rV5 ORF123 / hypothetical protein
FV3 ORF116 / rV5 ORF124 / hypothetical protein
FV3 ORF117 / rV5 ORF125 / hypothetical protein
FV3 ORF118 / rV5 ORF126 / hypothetical protein
FV3 ORF119 / rV5 ORF127 / hypothetical protein
FV3 ORF120 / rV5 ORF128 / hypothetical protein
FV3 ORF121 / rV5 ORF129 / hypothetical protein
FV3 ORF122 / rV5 ORF130 / hypothetical protein
FV3 ORF123 / rV5 ORF131 / hypothetical protein
FV3 ORF124 / rV5 ORF132 / hypothetical protein
FV3 ORF125 / rV5 ORF133 / possiblestructuralprotein
FV3 ORF126 / rV5 ORF134 / hypothetical protein
FV3 ORF127 / rV5 ORF135 / hypothetical protein
FV3 ORF128 / rV5 ORF136 / hypothetical protein
FV3 ORF129 / rV5 ORF137 / hypothetical protein
FV3 ORF130 / rV5 ORF138 / hypothetical protein
FV3 ORF131 / rV5 ORF139 / hypothetical protein
FV3 ORF132 / rV5 ORF140 / hypothetical protein
FV3 ORF133 / rV5 ORF141 / hypothetical protein
FV3 ORF134 / rV5 ORF142 / hypothetical protein
FV3 ORF135 / rV5 ORF143 / hypothetical protein
FV3 ORF136 / rV5 ORF144 / sigma54modulationfactor
FV3 ORF137 / rV5 ORF145 / hypothetical protein
FV3 ORF138 / rV5 ORF146 / hypothetical protein
FV3 ORF139 / rV5 ORF147 / hypothetical protein
FV3 ORF140 / rV5 ORF148 / hypothetical protein
FV3 ORF141 / rV5 ORF149 / hypothetical protein
FV3 ORF142 / rV5 ORF150 / hypothetical protein
FV3 ORF143 / rV5 ORF151 / hypothetical protein
FV3 ORF144 / rV5 ORF152 / hypothetical protein
FV3 ORF145 / rV5 ORF153 / hypothetical protein
FV3 ORF146 / rV5 ORF155 / hypothetical protein
FV3 ORF147 / rV5 ORF156 / hypothetical protein
FV3 ORF148 / rV5 ORF157 / hypothetical protein
FV3 ORF149 / rV5 ORF158 / hypothetical protein
FV3 ORF150 / rV5 ORF159 / hypothetical protein
FV3 ORF151 / rV5 ORF160 / hypothetical protein
FV3 ORF152 / rV5 ORF161 / hypothetical protein
FV3 ORF153 / rV5 ORF162 / hypothetical protein
FV3 ORF154 / rV5 ORF163 / hypothetical protein
FV3 ORF155 / rV5 ORF165 / hypothetical protein
FV3 ORF156 / rV5 ORF167 / hypothetical protein
FV3 ORF157 / rV5 ORF170 / hypothetical protein
FV3 ORF158 / rV5 ORF171 / hypothetical protein
FV3 ORF159 / rV5 ORF172 / hypothetical protein
FV3 ORF160 / rV5 ORF173 / hypothetical protein
FV3 ORF161 / unique / hypothetical protein
FV3 ORF162 / unique / hypothetical protein
FV3 ORF163 / rV5 ORF175 / hypothetical protein
FV3 ORF164 / rV5 ORF176 / hypothetical protein
FV3 ORF165 / rV5 ORF177 / hypothetical protein
FV3 ORF166 / rV5 ORF179 / hypothetical protein
FV3 ORF167 / rV5 ORF181 / hypothetical protein
FV3 ORF168 / rV5 ORF182 / hypothetical protein
FV3 ORF169 / rV5 ORF183 / conservedmembraneprotein
FV3 ORF170 / rV5 ORF184 / hypothetical protein
FV3 ORF171 / rV5 ORF185 / hypothetical protein
FV3 ORF172 / rV5 ORF186 / hypothetical protein
FV3 ORF173 / rV5 ORF189 / hypothetical protein
FV3 ORF174 / rV5 ORF190 / hypothetical protein
FV3 ORF175 / rV5 ORF191 / hypothetical protein
FV3 ORF176 / rV5 ORF192 / hypothetical protein
FV3 ORF177 / rV5 ORF193 / hypothetical protein
FV3 ORF178 / rV5 ORF194 / hypothetical protein
FV3 ORF179 / rV5 ORF195 / hypothetical protein
FV3 ORF180 / rV5 ORF196 / hypothetical protein
FV3 ORF181 / rV5 ORF197 / hypothetical protein
FV3 ORF182 / rV5 ORF198 / hypothetical protein
FV3 ORF183 / rV5 ORF199 / hypothetical protein
FV3 ORF184 / rV5 ORF200 / hypothetical protein
FV3 ORF185 / rV5 ORF201 / hypothetical protein
FV3 ORF186 / rV5 ORF203 / hypothetical protein
FV3 ORF187 / rV5 ORF204 / hypothetical protein
FV3 ORF188 / rV5 ORF205 / conservedhypotheticalphageprotein
FV3 ORF189 / rV5 ORF206 / hypothetical protein
FV3 ORF190 / rV5 ORF208 / hypothetical protein
FV3 ORF191 / rV5 ORF209 / hypothetical protein
FV3 ORF192 / rV5 ORF210 / hypothetical protein
FV3 ORF193 / rV5 ORF211 / hypothetical protein
FV3 ORF194 / rV5 ORF212 / hypothetical protein
FV3 ORF195 / rV5 ORF214 / hypothetical protein
FV3 ORF196 / rV5 ORF215 / hypothetical protein
FV3 ORF197 / rV5 ORF216 / hypothetical protein
FV3 ORF198 / rV5 ORF217 / hypothetical protein
FV3 ORF199 / rV5 ORF218 / hypothetical protein
FV3 ORF200 / rV5 ORF219 / hypothetical protein
FV3 ORF201 / rV5 ORF220 / hypothetical protein
FV3 ORF202 / rV5 ORF221 / hypothetical protein
FV3 ORF203 / rV5 ORF222 / hypothetical protein
FV3 ORF204 / rV5 ORF223 / hypothetical protein
FV3 ORF205 / rV5 ORF224 / hypothetical protein
FV3 ORF206 / rV5 ORF225 / hypothetical protein
FV3 ORF207 / rV5 ORF226 / hypothetical protein
FV3 ORF208 / rV5 ORF65 / HNHhomingendonuclease
FV3 ORF209 / rV5 ORF228 / DNApolymerase
FV3 ORF210 / rV5 ORF230 / DNAreplicativehelicase/primase
FV3 ORF211 / rV5 ORF231 / hypothetical protein
FV3 ORF212 / rV5 ORF232 / hypothetical protein
FV3 ORF213 / rV5 ORF233 / hypothetical protein
FV3 ORF214 / rV5 ORF234 / hypothetical protein
FV3 ORF215 / rV5 ORF235 / adenine methyltransferase
FV3 ORF216 / rV5 ORF236 / hypothetical protein
FV3 ORF217 / rV5 ORF237 / helicase
FV3 ORF218 / rV5 ORF238 / RIIB protein

* identified by homology tothe PVP-SE1 virionproteins determined by mass spectrometry analysis.