Table 1a: Data Collection, phasing and refinement statistics of full-length Trigger Factor crystals.

Data Collection / Inflection / Peak / Remote /

Refinement

Cell Parameters (Å) / a=100.2 b=47.4 c=114.8
b=113.6 / R, R free / 0.241, 0.324
Wavelength (Å) / 0.97931 / 0.97879 / 0.97095 / r.m.s.d. bonds (Å) / 0.007
Mosaicity (°) / 0.383 / 0.434 / 0.550 / r.m.s.d. angles (°) / 1.31
Total Reflections / 128033 / 124975 / 88713 / Luzzati error e (Å) / 0.39
Unique Reflections / 27546 / 26426 / 19366 / Monomers / a.u. f / 2
Resolution (Å) / 50 - 2.7 / 50 - 2.7 / 50 – 3.0 / Number of protein residues / 864
Rsym (%) a,b / 4.4 (69.1) / 6.3 (58.8) / 4.4 (70.0) / Number of protein atoms / 6787
Completeness (%) a / 100 (91.4) / 100 (99.5) / 99.9 (99.4) / Number of water molecules / 88
I / sigma a / 26.1 (1.8) / 15.8 (2.8) / 23.0 (2.6)
f' / -10.02 / -7.9 / -4.8
f" / 3.1 / 4.92 / 3.8
Phasing power c / 1.535 / 1.922 / 1.278 / Solvent content (%) / 56
Combined FOM d / 0.322 / 0.379 / 0.286 / áB total ñ (Å2) / 74
Number of sites / 19
a Values for highest resolution shells are given in parentheses.
b Rsym=S| Iobs - áIñ| / SáIñ
c Phasing power (centric / acentric) = r.m.s. Fh / Eiso, where Eiso is the lack of closure error.
d FOM, figure of merit. FOM = ácos (Dah)ñ, where Dah is the error in the phase angle for reflection h
e luzzati coordinate error (5.0 – 2.7 Å)
f a.u. = asymmetric unit

Table 1b: Data Collection and refinement statistics of the Trigger Factor fragment TF 1-144 in

complex with the Haloarcula marismortui 50S ribosomal subunit.

Data Collection / Refinement
Cell Parameters (Å) / a=211 b=299 c=574 / R, R free / 0.192, 0.268
Wavelength (Å) / 0.9000 / r.m.s.d. bonds (Å) / 0.006
Mosaicity (°) / 0.790 / r.m.s.d. angles (°) / 1.2
Total Reflections / 1014536
Unique Reflections / 234003 / Monomers / a.u. c / 1
Resolution (Å) / 50 - 3.5 / Solvent content (%) / 55
Rsym (%) a,b / 25.8 (69.1)
Completeness (%) a / 96.8 (94.2)
I / sigma a / 25.4 (2.0)
a Values for highest resolution shells are given in parentheses.
b Rsym=S| Iobs - áIñ| / SáIñ
c a.u. = asymmetric unit