Table 1 Phylogenetic Affiliation of DGGE Bands of Activated Sludge and Endophytic Bacterial
Supplementary data
Table 1 Phylogenetic affiliation of DGGE bands of activated sludge and endophytic bacterial samples.
Fig. 1 Regenerated plants from synthetic seeds under greenhouse conditions (A) and in the simulated wetland (B, C).
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Table 1 Phylogenetic affiliation of DGGE bands of activated sludge and endophytic bacterial samples
Band / Lane / Closest organism in GenBank database / Accession no. / Similarity (%) / Taxonomic groupIAS / MAS / EB
1 / － / ＋ / ＋ / Bacillus licheniformis strain A39-1 (KM099482.1) / KX154715 / 100 / Firmicutes
2 / ＋ / ＋ / ＋ / Klebsiella pneumoniae strain 125 (KM377646.1) / KX154716 / 100 / Gammaproteobacteria
3 / ＋ / ＋ / － / Uncultured bacterium (EF605855.1) / KX154717 / 97 / Uncultured bacterium
4 / ＋ / ＋ / － / Rhodococcus cerastii strain C5 (FR714842) / KX154718 / 100 / Actinobacteria
5 / － / ＋ / ＋ / Corynebacterium coyleae strain DSM 44184 ( X96497) / KX154719 / 99.42 / Actinobacteria
6 / － / － / ＋ / Novosphingobium sediminicola strain HU1-AH51 (FJ177534) / KX154720 / 98.82 / Alphaproteobacteria
7 / － / ＋ / ＋ / Albidiferax ferrireducens strain CH1-46 (KC855480.1) / KX154721 / 98.45 / Betaproteobacteria
8 / － / － / ＋ / Lactobacillus iners strain DSM 13335 (ACLN01000018) / KX154722 / 100 / Firmicutes
9 / － / － / ＋ / Microbacterium imperiale strain DSM 20530 (X77442) / KX154723 / 100 / Actinobacteria
10 / ＋ / ＋ / ＋ / Staphylococcus epidermidis strain P4 (KF705246.1) / KX154724 / 100 / Firmicutes
11 / ＋ / － / ＋ / Uncultured bacterium (HM668467.1) / KX154725 / 98 / Uncultured bacterium
12 / － / ＋ / ＋ / Nakamurella multipartita strain DSM 44233 (CP001737) / KX154726 / 100 / Actinobacteria
13 / ＋ / ＋ / ＋ / Desulfuromonas acetexigens strain DSM 1397 (U23140) / KX154727 / 98.97 / Deltaproteobacteria
14 / ＋ / ＋ / ＋ / Flavobacterium johnsoniae strain UW101 (CP000685) / KX154728 / 99.47 / Flavobacteria
15 / － / ＋ / ＋ / Kocuria rhizophila strain DSM 11926 (Y16264) / KX154729 / 99.43 / Actinobacteria
16 / ＋ / ＋ / ＋ / Clostridium clostridioforme strain ATCC 25537 (M59089) / KX154730 / 98.22 / Firmicutes
17 / ＋ / － / ＋ / Acinetobacter oleivorans strain DR1 (CP002080) / KX154731 / 98.97 / Gammaproteobacteria
18 / － / － / ＋ / Geobacter daltonii strain FRC-32 (CP001390) / KX154732 / 95.90 / Deltaproteobacteria
19 / ＋ / ＋ / － / Thermomonas fusca strain DSM 15424 (AUIV01000012) / KX154733 / 98.97 / Gammaproteobacteria
20 / ＋ / － / ＋ / Pseudomonas psychrotolerans strain C36 (AJ575816) / KX154734 / 100 / Gammaproteobacteria
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Table 1 (continued)
Band / Lane / Closest organism in GenBank database / Accession no. / Similarity (%) / Taxonomic groupIAS / MAS / EB
21 / － / ＋ / － / Actinomyces meyeri strain CIP 103148 (X82451) / KX154735 / 98.40 / Firmicutes
22 / ＋ / ＋ / － / Prevotella multiformis strain DSM 16608 (GL872289) / KX154736 / 99.47 / Bacteroidetes
23 / ＋ / ＋ / － / Azoarcus buckelii strain U120 (AJ315676) / KX154737 / 94.33 / Betaproteobacteria
24 / ＋ / ＋ / － / Micropruina glycogenica strain Lg2 (AB012607) / KX154738 / 99.41 / Actinobacteria
25 / ＋ / ＋ / ＋ / Prevotella melaninogenica strain ATCC 25845 (ACSI01000031) / KX154739 / 99.47 / Bacteroidetes
26 / ＋ / － / ＋ / Prevotella buccae strain ATCC 33574 (L16477) / KX154740 / 98.92 / Bacteroidetes
27 / ＋ / ＋ / ＋ / Aquincola tertiaricarbonis strain L10 (DQ656489) / KX154741 / 90.72 / Betaproteobacteria
28 / ＋ / ＋ / ＋ / Moraxella osloensis strain NCTC 10465 (X74897) / KX154742 / 100 / Gammaproteobacteria
29 / － / ＋ / － / Acinetobacter refrigeratorensis strain WB1 (KF709454) / KX154743 / 98.97 / Gammaproteobacteria
30 / ＋ / ＋ / ＋ / Massilia suwonensis strain 5414S-25 (FJ969487) / KX154744 / 98.97 / Betaproteobacteria
31 / － / ＋ / ＋ / Dokdonella immobilis strain LM 2-5 (FJ455531) / KX154745 / 95.36 / Gammaproteobacteria
32 / ＋ / ＋ / － / Pelomonas puraquae strain CCUG 52769 (AM501439) / KX154746 / 92.78 / Betaproteobacteria
33 / － / ＋ / ＋ / Actinokineospora cianjurensis strain ID03-0810 (AB473945) / KX154747 / 100 / Actinobacteria
34 / ＋ / ＋ / ＋ / Enterobacter soli strain LF7a (CP003026) / KX154748 / 100 / Gammaproteobacteria
35 / ＋ / ＋ / ＋ / Cellulomonas chitinilytica strain X.bu-b (AB268586) / KX154749 / 100 / Actinobacteria
36 / ＋ / ＋ / － / Ardenticatena maritima strain 110S (AB576167) / KX154750 / 92.31 / Chloroflexi
37 / ＋ / ＋ / ＋ / Corynebacterium tuberculostearicum strain CIP107291 (AJ438050) / KX154751 / 99.43 / Actinobacteria
38 / ＋ / ＋ / － / Uncultured bacterium (HM694801.1) / KX154752 / 99 / Uncultured bacterium
39 / ＋ / ＋ / ＋ / Anaerobiospirillum succiniciproducens strain ATCC 29305 (U96412) / KX154753 / 94.15 / Gammaproteobacteria
40 / ＋ / － / － / Streptococcus equinus strain ATCC 9812 (GL698435) / KX154754 / 99.49 / Firmicutes
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Table 1 (continued)
Band / Lane / Closest organism in GenBank database / Accession no. / Similarity (%) / Taxonomic groupIAS / MAS / EB
41 / ＋ / ＋ / ＋ / Fusobacterium nucleatum strain YWH7415 (KF444266.1) / KX154695 / 100 / Fusobacteria
42 / ＋ / ＋ / ＋ / Arcobacter nitrofigilis strain DSM 7299 (CP001999) / KX154696 / 99.41 / Epsilonproteobacteria
43 / ＋ / ＋ / － / Brevundimonas staleyi strain FWC43 (AJ227798) / KX154697 / 99.41 / Alphaproteobacteria
44 / ＋ / ＋ / － / Kallotenue papyrolyticum strain JKG1 (JX848544) / KX154698 / 92.31 / Chloroflexi
45 / ＋ / ＋ / － / Streptococcus intermedius strain SK54 (AJKN01000012) / KX154699 / 100 / Firmicutes
46 / ＋ / － / － / Simonsiella muelleri strain ATCC 29453 (ADCY01000105) / KX154700 / 93.30 / Betaproteobacter
47 / ＋ / ＋ / － / Corynebacterium matruchotii strain ATCC 14266 (ACSH01000013) / KX154701 / 100 / Actinobacteria
48 / ＋ / ＋ / － / Granulicatella adiacens strain ATCC 49175 (ACKZ01000002) / KX154702 / 100 / Firmicutes
49 / ＋ / ＋ / － / Streptococcus pneumoniae strain ATCC 33400 (AF003930) / KX154703 / 100 / Firmicutes
50 / － / － / ＋ / Prolinoborus fasciculus strain CIP 103579 (JN175353) / KX154704 / 100 / Gammaproteobacteria
51 / ＋ / ＋ / ＋ / Methylobacterium phyllosphaerae strain CBMB27 (EF126746) / KX154705 / 99.41 / Alphaproteobacteria
52 / ＋ / ＋ / ＋ / Azospirillum brasilense strain ATCC 49958 (AY150046) / KX154706 / 96.45 / Alphaproteobacteria
53 / ＋ / ＋ / － / Neisseria oralis strain 6332 (JN104029) / KX154707 / 97.94 / Betaproteobacteria
54 / ＋ / ＋ / － / Diaphorobacter nitroreducens strain NA10B (AB064317) / KX154708 / 100 / Betaproteobacteria
55 / ＋ / ＋ / － / Anaerococcus hydrogenalis strain DSM 7454 (ABXA01000039) / KX154709 / 100 / Firmicutes
56 / ＋ / ＋ / － / Dolosigranulum pigrum strain ATCC 51524 (JH601103) / KX154710 / 100 / Firmicutes
57 / ＋ / ＋ / － / Herbaspirillum frisingense strain GSF30 (AEEC01001472) / KX154711 / 99.33 / Betaproteobacteria
58 / ＋ / ＋ / － / Hymenobacter norwichensis strain NS/50 (AJ549285) / KX154712 / 100 / Bacteroidetes
59 / ＋ / ＋ / － / Herpetosiphon aurantiacus strain ATCC 23779 (CP000875) / KX154713 / 92.31 / Chloroflexi
60 / ＋ / ＋ / － / Sphingomonas endophytica strain YIM 65583 (HM629444) / KX154714 / 100 / Alphaproteobacteria
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Abbreviations: IAS-initial activated sludge, MAS-symbiotic activated sludge, EB-endophytic bacteria.
+, visible band; -, no band.
Fig. 1 Regenerated plants from synthetic seeds under greenhouse conditions (A) and in the simulated wetland (B, C).
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