SUPPORTING INFORMATION
Homo- and heteromeric interaction strengths of the synergistic antimicrobial peptides PGLa and magainin 2 in membranes
Jonathan Zerweck,‡ Erik Strandberg,† Jochen Bürck,† Johannes Reichert,† Parvesh Wadhwani,† Olga Kukharenko,‡ and Anne S. Ulrich†,‡,*
† Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany; ‡ KIT, Institute of Biological Interfaces (IBG-2), POB 3640, 76021 Karlsruhe, Germany
*Correspondence: Anne S. Ulrich. Email:
Table S1. 19F-NMR splittings of 19F-labeled MAG2, in the absence and presence of PGLa, in oriented bilayers of DMPC/DMPG (3/1). The total peptide-to-lipid molar ratio (P/L) and the corresponding peptide-to-peptide-to-lipid molar ratio (P/P/L) are given. Samples were measured with the membrane normal aligned parallel to the magnetic field (0°) and perpendicular to the magnetic field (90°).
Peptide(s) / P/L / P/P/L / 0° [kHz] / 90° [kHz]MAG2-19F-V17 / 1/50 / - / -3.7 / 1.7
1/500 / - / -4.3 / 2.2
MAG2-19F-V17/PGLa / 1/50 / 1/1/100 / -6.6 / 3.3
1/100 / 1/1/200 / -7.0 (2.6) / 3.5 (-1.2)
1/200 / 1/1/400 / -6.9 (-3.7) / -
1/300 / 1/1/600 / -6.3 (-4.4) / 2.0
1/500 / 1/1/1000 / -4.3 / 2.0
MAG2-19F-G18 / 1/50 / - / 2.8 / -1.6
1/500 / - / 5.0 / -2.2
MAG2-19F-G18/PGLa / 1/50 / 1/1/100 / 12.7 / -6.1
1/200 / 1/1/400 / (13.5) / -6.2
1/500 / 1/1/1000 / 2.6 / -1.7
Fig. S1 31P-NMR spectra of the oriented DMPC/DMPG (3/1) phospholipid bilayers containing MAG2-19F-V17, measured at 0° and 90° sample alignment at different peptide concentrations, with and without PGLa. These spectra served to check the sample quality before and after the corresponding 19F-NMR measurement. (A) P/L = 1/50; (B) P/L = 1/500; (C) +PGLa, P/P/L = 1/1/1000; (D) +PGLa, P/P/L = 1/1/600; (E) +PGLa, P/P/L = 1/1/400; (F) +PGLa, P/P/L = 1/1/200; (G) +PGLa, P/L = 1/1/100
Fig. S2 19F-NMR spectra of MAG2-19F-G18 in oriented DMPC/DMPG (3/1) lipid bilayers at different concentrations, with and without PGLa. The red dashed line indicates the isotropic chemical shift at -72 ppm. In every sample, there is a large splitting of -7.6 kHz centred around -71 ppm, which can be attributed to immobilized (probably aggregated) peptides (Wadhwani et al. 2008). However, in addition to this splitting, there is also a signal from the relevant mobile MAG2 molecules (grey background). (A) P/L = 1/50; (B) P/P/L = 1/500; (C) +PGLa, P/P/L = 1/1/1000; (D) +PGLa, P/P/L = 1/1/400; (E) +PGLa, P/P/L = 1/1/100
3