Supplementary Table S5.Results of Gene Ontology analysis of genes (twofold) differentially expressed in ursolic acid (UA)-treated S. mutans UA159

Term / Count / % / PValue / Genes / List Total / Pop Hits / Pop Total / Fold Enrichment / Bonferroni / Benjamini / FDR
Two-fold up- regulated genes
GO:0019219~regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process / 45 / 10.04464 / 5.40E-06 / 4603312, 4602586, 4603012, 4603051, 4602584, 4602549, 4603911, 4603071, 4603212, 4603230, 4603437, 4604386, 4603745, 4603053, 4604082, 4603282, 4604454, 4602904, 4604001, 4602903, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603793, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602650, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 117 / 825 / 1.823607427 / 8.80E-04 / 8.80E-04 / 0.0065
GO:0045449~regulation of transcription / 43 / 9.598214 / 2.30E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603071, 4603212, 4603230, 4603437, 4604386, 4603745, 4603053, 4604082, 4603282, 4604454, 4602904, 4604001, 4602903, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603793, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 115 / 825 / 1.772863568 / 0.0037427 / 0.001873 / 0.0279
GO:0031326~regulation of cellular biosynthetic process / 44 / 9.821429 / 2.45E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603071, 4603212, 4603230, 4603437, 4604386, 4603745, 4603053, 4604082, 4603282, 4604454, 4602904, 4604001, 4602903, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603793, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602650, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 119 / 825 / 1.753115039 / 0.0039829 / 0.001329 / 0.0297
GO:0010556~regulation of macromolecule biosynthetic process / 44 / 9.821429 / 2.45E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603071, 4603212, 4603230, 4603437, 4604386, 4603745, 4603053, 4604082, 4603282, 4604454, 4602904, 4604001, 4602903, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603793, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602650, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 119 / 825 / 1.753115039 / 0.0039829 / 0.001329 / 0.0297
GO:0006355~regulation of transcription, DNA-dependent / 40 / 8.928571 / 5.22E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603212, 4603230, 4603071, 4603437, 4604386, 4603745, 4603053, 4603282, 4604454, 4602904, 4604001, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 107 / 825 / 1.772478247 / 0.0084735 / 0.002125 / 0.0632
GO:0051252~regulation of RNA metabolic process / 40 / 8.928571 / 5.22E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603212, 4603230, 4603071, 4603437, 4604386, 4603745, 4603053, 4603282, 4604454, 4602904, 4604001, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 107 / 825 / 1.772478247 / 0.0084735 / 0.002125 / 0.0632
GO:0010468~regulation of gene expression / 43 / 9.598214 / 9.84E-05 / 4603312, 4603012, 4603051, 4602584, 4602549, 4603911, 4603071, 4603212, 4603230, 4603437, 4604386, 4603745, 4603053, 4604082, 4603282, 4604454, 4602904, 4604001, 4602903, 4603141, 4602992, 4603180, 4603943, 4603769, 4604048, 4603793, 4603221, 4604233, 4602552, 4604258, 4602781, 4602860, 4604424, 4602593, 4604354, 4604155, 4602931, 4603135, 4603303, 4603155, 4602726, 4604515, 4603005 / 174 / 121 / 825 / 1.684952978 / 0.0159119 / 0.003203 / 0.1191
GO:0006457~protein folding / 7 / 1.5625 / 0.01413146 / 4604200, 4603453, 4603959, 4603698, 4603626, 4604124, 4603615 / 174 / 11 / 825 / 3.017241379 / 0.9017125 / 0.320667 / 15.836
GO:0009306~protein secretion / 5 / 1.116071 / 0.01971455 / 4602982, 4602908, 4604385, 4602542, 4603043 / 174 / 6 / 825 / 3.951149425 / 0.9610536 / 0.371018 / 21.431
GO:0032940~secretion by cell / 5 / 1.116071 / 0.01971455 / 4602982, 4602908, 4604385, 4602542, 4603043 / 174 / 6 / 825 / 3.951149425 / 0.9610536 / 0.371018 / 21.431
GO:0042398~cellular amino acid derivative biosynthetic process / 6 / 1.339286 / 0.02321504 / 4603450, 4602913, 4602946, 4602936, 4604498, 4603320 / 174 / 9 / 825 / 3.16091954 / 0.9782614 / 0.380339 / 24.763
GO:0030001~metal ion transport / 9 / 2.008929 / 0.03964295 / 4604155, 4602599, 4604491, 4603871, 4603369, 4603901, 4604312, 4602723, 4604216 / 174 / 20 / 825 / 2.13362069 / 0.9986306 / 0.519341 / 38.736
GO:0006576~biogenic amine metabolic process / 5 / 1.116071 / 0.06467322 / 4603450, 4602913, 4602946, 4602936, 4603320 / 174 / 8 / 825 / 2.963362069 / 0.9999815 / 0.663719 / 55.509
GO:0042401~biogenic amine biosynthetic process / 5 / 1.116071 / 0.06467322 / 4603450, 4602913, 4602946, 4602936, 4603320 / 174 / 8 / 825 / 2.963362069 / 0.9999815 / 0.663719 / 55.509
GO:0009401~phosphoenolpyruvate-dependent sugar phosphotransferase system / 13 / 2.901786 / 0.06585624 / 4603860, 4602700, 4602995, 4604105, 4602570, 4602608, 4604550, 4602980, 4604557, 4603267, 4603141, 4603318, 4603768 / 174 / 37 / 825 / 1.665890028 / 0.999985 / 0.635594 / 56.186
Two-fold down- regulated genes
GO:0015986~ATP synthesis coupled proton transport / 7 / 1.639344 / 7.23E-04 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 7 / 825 / 4.388297872 / 0.1358716 / 0.135872 / 0.9035
GO:0015985~energy coupled proton transport, down electrochemical gradient / 7 / 1.639344 / 7.23E-04 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 7 / 825 / 4.388297872 / 0.1358716 / 0.135872 / 0.9035
GO:0015992~proton transport / 7 / 1.639344 / 7.23E-04 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 7 / 825 / 4.388297872 / 0.1358716 / 0.135872 / 0.9035
GO:0006820~anion transport / 7 / 1.639344 / 7.23E-04 / 4603681, 4603105, 4602834, 4602873, 4603353, 4604333, 4603399 / 188 / 7 / 825 / 4.388297872 / 0.1358716 / 0.135872 / 0.9035
GO:0034220~ion transmembrane transport / 7 / 1.639344 / 0.00234523 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 8 / 825 / 3.839760638 / 0.3776753 / 0.211124 / 2.9047
GO:0015698~inorganic anion transport / 6 / 1.405152 / 0.00280627 / 4603681, 4603105, 4602834, 4602873, 4603353, 4604333 / 188 / 6 / 825 / 4.388297872 / 0.4331518 / 0.172397 / 3.4665
GO:0016310~phosphorylation / 14 / 3.278689 / 0.00405532 / 4603783, 4602642, 4604553, 4603361, 4602789, 4604546, 4603677, 4602862, 4603806, 4602746, 4602541, 4603789, 4603881, 4603080 / 188 / 28 / 825 / 2.194148936 / 0.559938 / 0.185524 / 4.9736
GO:0008299~isoprenoid biosynthetic process / 6 / 1.405152 / 0.00801525 / 4604057, 4604397, 4603677, 4603806, 4604063, 4603092 / 188 / 7 / 825 / 3.761398176 / 0.8032074 / 0.27756 / 9.6096
GO:0006720~isoprenoid metabolic process / 6 / 1.405152 / 0.00801525 / 4604057, 4604397, 4603677, 4603806, 4604063, 4603092 / 188 / 7 / 825 / 3.761398176 / 0.8032074 / 0.27756 / 9.6096
GO:0009144~purine nucleoside triphosphate metabolic process / 7 / 1.639344 / 0.0207865 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 11 / 825 / 2.792553191 / 0.9856374 / 0.506971 / 23.181
GO:0022604~regulation of cell morphogenesis / 10 / 2.34192 / 0.02979151 / 4602894, 4603885, 4603742, 4603423, 4603025, 4602756, 4604335, 4603393, 4604024, 4603092 / 188 / 21 / 825 / 2.089665653 / 0.997778 / 0.582205 / 31.593
GO:0008360~regulation of cell shape / 10 / 2.34192 / 0.02979151 / 4602894, 4603885, 4603742, 4603423, 4603025, 4602756, 4604335, 4603393, 4604024, 4603092 / 188 / 21 / 825 / 2.089665653 / 0.997778 / 0.582205 / 31.593
GO:0015672~monovalent inorganic cation transport / 8 / 1.873536 / 0.03341275 / 4602789, 4602862, 4602746, 4602642, 4602541, 4604312, 4603789, 4603881 / 188 / 15 / 825 / 2.340425532 / 0.998956 / 0.576028 / 34.73
GO:0009142~nucleoside triphosphate biosynthetic process / 7 / 1.639344 / 0.03386781 / 4602789, 4602862, 4602746, 4602642, 4602541, 4603789, 4603881 / 188 / 12 / 825 / 2.559840426 / 0.9990507 / 0.538519 / 35.115
GO:0070589~cellular component macromolecule biosynthetic process / 10 / 2.34192 / 0.04075939 / 4602894, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092, 4602950 / 188 / 22 / 825 / 1.994680851 / 0.9997765 / 0.568544 / 40.692
GO:0043038~amino acid activation / 10 / 2.34192 / 0.05417412 / 4603516, 4603631, 4603031, 4604431, 4602652, 4603179, 4603300, 4604159, 4603095, 4602801 / 188 / 23 / 825 / 1.907955597 / 0.999987 / 0.640412 / 50.304
GO:0006418~tRNA aminoacylation for protein translation / 10 / 2.34192 / 0.05417412 / 4603516, 4603631, 4603031, 4604431, 4602652, 4603179, 4603300, 4604159, 4603095, 4602801 / 188 / 23 / 825 / 1.907955597 / 0.999987 / 0.640412 / 50.304
GO:0009252~peptidoglycan biosynthetic process / 9 / 2.107728 / 0.060288 / 4602894, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092 / 188 / 20 / 825 / 1.974734043 / 0.9999965 / 0.648915 / 54.189
GO:0006023~aminoglycan biosynthetic process / 9 / 2.107728 / 0.060288 / 4602894, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092 / 188 / 20 / 825 / 1.974734043 / 0.9999965 / 0.648915 / 54.189
GO:0006024~glycosaminoglycan biosynthetic process / 9 / 2.107728 / 0.060288 / 4602894, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092 / 188 / 20 / 825 / 1.974734043 / 0.9999965 / 0.648915 / 54.189
GO:0006812~cation transport / 12 / 2.810304 / 0.069072 / 4603281, 4602789, 4602785, 4602862, 4604276, 4602746, 4602642, 4604292, 4602541, 4604312, 4603789, 4603881 / 188 / 31 / 825 / 1.698695951 / 0.9999995 / 0.671145 / 59.284
GO:0006022~aminoglycan metabolic process / 10 / 2.34192 / 0.07017812 / 4602894, 4603254, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092 / 188 / 24 / 825 / 1.828457447 / 0.9999996 / 0.650012 / 59.888
GO:0030203~glycosaminoglycan metabolic process / 10 / 2.34192 / 0.07017812 / 4602894, 4603254, 4603885, 4603742, 4603423, 4603025, 4604335, 4603393, 4604024, 4603092 / 188 / 24 / 825 / 1.828457447 / 0.9999996 / 0.650012 / 59.888
GO:0009396~folic acid and derivative biosynthetic process / 5 / 1.17096 / 0.0830475 / 4603199, 4602698, 4602934, 4603580, 4602897 / 188 / 8 / 825 / 2.74268617 / 1 / 0.688873 / 66.327

461 and 446 geneswere up- and down-regulated by UA, respectively. These genes were mined in the Database for Annotation, Visualization and Integrated Discovery (DAVID) tool ( and the UniProt-GO Annotation database (