Supplementary Table S5 Experiment 2.2 Absolute call rates for 50-kHz call subtypes

CX / UR / DR / FL / SH / SP / SU / SD / MS / TR / FT / TJ / IU / CS / UC / MI
Saline
Median / 2.8 / 1.8 / 1.8 / 6.3 / 1.5 / 0 / 4.5 / 0.5 / 1 / 7.5 / 2.3 / 0.3 / 0.8 / 2 / 3.8 / 0
Mean / 5.7 / 5.7 / 2 / 14.8 / 2.3 / 0.4 / 8.8 / 1.5 / 2.6 / 11.5 / 6.3 / 1.7 / 3.1 / 4.1 / 6.9 / 0.1
Std. Error / 1.9 / 2.1 / 0.4 / 5 / 0.6 / 0.2 / 3.3 / 0.6 / 1.2 / 3.5 / 2.7 / 0.8 / 1.6 / 1.2 / 2.3 / 0.1
Minimum / 0 / 0 / 1 / 1 / 0 / 0 / 0.5 / 0 / 0 / 0.5 / 0 / 0 / 0 / 0.5 / 0.5 / 0
Maximum / 25.5 / 28 / 7.5 / 67.5 / 7 / 2.5 / 48 / 7 / 17 / 48 / 29.5 / 9.5 / 23.5 / 13.5 / 33 / 0.5
Morphine 0.1 mg/kg
Median / 1.5 / 2.5 / 3 / 8 / 2.5 / 0 / 6 / 0 / 0.5 / 9 / 2.5 / 0.5 / 2 / 2 / 4.5 / 0
Mean / 5.3 / 6.3 / 3.2 / 11.8 / 2.4 / 0.1 / 7.1 / 1.2 / 1.9 / 14.3 / 5.1 / 2.3 / 2.5 / 5.9 / 8.3 / 0
Std. Error / 1.9 / 2.2 / 0.6 / 3.3 / 0.6 / 0.1 / 2.2 / 0.6 / 0.8 / 5.2 / 1.8 / 1 / 0.6 / 2.3 / 2.8 / 0
Minimum / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 22 / 26 / 9 / 42 / 7 / 1 / 32 / 9 / 9 / 72 / 20 / 13 / 6 / 30 / 36 / 0
Morphine 0.3 mg/kg
Median / 4 / 5 / 2 / 11.5 / 2 / 0 / 7 / 1.5 / 2.5 / 28.5 / 7 / 1 / 1 / 4.5 / 12.5 / 0
Mean / 12.3 / 8.6 / 2.6 / 23.9 / 3.8 / 0.8 / 12.2 / 3.1 / 3.7 / 42.1 / 12.5 / 4.6 / 4.7 / 7.9 / 16.8 / 0.6
Std. Error / 5.5 / 2.7 / 0.7 / 7.7 / 1 / 0.4 / 3.9 / 1.4 / 1.2 / 11.8 / 4.6 / 2.2 / 1.8 / 2.2 / 4.5 / 0.2
Minimum / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 78 / 28 / 8 / 88 / 9 / 5 / 54 / 20 / 14 / 129 / 60 / 26 / 23 / 26 / 47 / 2
Morphine 1 mg/kg
Median / 8.5 / 5.5 / 4.5 / 22 / 3 / 1 / 10 / 2 / 5 / 25.5 / 5 / 2.5 / 6.5 / 11.5 / 13.5 / 0
Mean / 19.9 / 9.1 / 5.7 / 22.8 / 3.5 / 2.4 / 16.9 / 4.5 / 6.3 / 53 / 17.4 / 8.3 / 10.8 / 17.6 / 21.5 / 0.6
Std. Error / 7.3 / 2.9 / 0.9 / 5.1 / 0.8 / 1 / 6.3 / 1.5 / 1.8 / 19.9 / 7.8 / 4.6 / 3.8 / 4.9 / 6 / 0.2
Minimum / 1 / 0 / 1 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 91 / 33 / 12 / 61 / 10 / 12 / 90 / 19 / 24 / 262 / 101 / 66 / 51 / 65 / 75 / 2
Morphine 3 mg/kg
Median / 5 / 2 / 1.5 / 4 / 0.5 / 0 / 1.5 / 1 / 1.5 / 8 / 2 / 0 / 1.5 / 3.5 / 3 / 0
Mean / 6.8 / 2.9 / 2.8 / 7.7 / 1.3 / 0.1 / 4.1 / 2 / 1.3 / 17.6 / 5.4 / 1.4 / 2.6 / 5.6 / 8.1 / 0
Std. Error / 1.8 / 0.7 / 0.8 / 2.5 / 0.4 / 0.1 / 1.5 / 0.6 / 0.3 / 5.6 / 2.3 / 0.8 / 0.9 / 1.5 / 2.6 / 0
Minimum / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 24 / 7 / 10 / 31 / 4 / 2 / 17 / 8 / 3 / 66 / 31 / 12 / 11 / 15 / 29 / 0

Notes:

Values represent the number of calls emitted per 20-min session for each call subtype. In general, the data were positively skewed, so that mean values tended to be larger than the corresponding medians. n=14 rats, repeated measures design. Call subtype abbreviations: CX complex, UR upward ramp, DR downward ramp, FL flat, SH short, SP split, SU step-up, SD step-down, MS multi-step, TR trill, FT flat-trill, TJ trill with jumps, IU inverted-U, CS composite, UC unclear, MI miscellaneous.Absolute emission rates were significantly increased not only for trills (0.3 and 1 mg/kg: Z=2.97 and 3.14, p=0.0030 and p=0.0017, respectively) but also for flat calls (1 mg/kg: Z=2.14, p=0.0326).