Supplementary Table S3 Experiment 1.1 Absolute call rates for 50-kHz call subtypes

CX / UR / DR / FL / SH / SP / SU / SD / MS / TR / FT / TJ / IU / CS / UC / MI
Days 1-4, Saline
Median / 4 / 2.8 / 2.8 / 13.3 / 3.3 / 0.3 / 3.8 / 1.5 / 0.8 / 6.5 / 2 / 0.5 / 2.8 / 2 / 0.5 / 0
Mean / 9.3 / 3.8 / 4.2 / 28.7 / 10.8 / 0.8 / 13.1 / 2.5 / 4.3 / 16.1 / 9.6 / 1.8 / 3.4 / 4.4 / 3.8 / 0
Std. Error / 2.7 / 1.2 / 1.1 / 7.3 / 4 / 0.3 / 4.3 / 0.6 / 1.6 / 5.4 / 4.5 / 0.7 / 0.8 / 1.3 / 1.3 / 0
Minimum / 0 / 0 / 0 / 3 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 30 / 19.5 / 19 / 95.5 / 64 / 4.5 / 58.5 / 6 / 22 / 65 / 68.5 / 7.5 / 13.5 / 18 / 14 / 0
Days 1-4, Morphine
Median / 4.5 / 1.5 / 1.3 / 12 / 2.5 / 0 / 4.3 / 3.3 / 0.8 / 4 / 1.5 / 0.3 / 2.3 / 1.8 / 0.5 / 0
Mean / 17.1 / 2.8 / 3 / 27.2 / 7.3 / 0.6 / 15.8 / 6.6 / 7.5 / 36.2 / 16.7 / 2.9 / 4.7 / 7.8 / 5.4 / 0
Std. Error / 5.7 / 0.8 / 0.8 / 8 / 2.2 / 0.2 / 5.5 / 2.4 / 2.4 / 17 / 9 / 1.3 / 1.4 / 2.8 / 2.1 / 0
Minimum / 0 / 0 / 0 / 0.5 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 72 / 9.5 / 9.5 / 88 / 27.5 / 3 / 60.5 / 32 / 27 / 255 / 142.5 / 15 / 18.5 / 35 / 27 / 0
Days 7-10, Saline
Median / 1.5 / 1.3 / 0.5 / 10 / 10.5 / 0 / 3 / 2 / 2 / 25.3 / 7.8 / 2.5 / 0 / 0 / 3.5 / 0.5
Mean / 3.3 / 2.6 / 1.4 / 25.4 / 16.3 / 0.3 / 9 / 3.1 / 4.1 / 38.1 / 28.6 / 6.2 / 0.7 / 0.3 / 5.3 / 0.7
Std. Error / 1 / 0.9 / 0.6 / 7.2 / 4.1 / 0.1 / 2.9 / 0.9 / 1 / 10.8 / 12.4 / 1.9 / 0.3 / 0.1 / 1.4 / 0.2
Minimum / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 15 / 11.5 / 7.5 / 96 / 56 / 1 / 43.5 / 14.5 / 13.5 / 166 / 191.5 / 22.5 / 4.5 / 2 / 18.5 / 3
Days 7-10, Morphine
Median / 3.8 / 1 / 0.5 / 15 / 10.5 / 0.5 / 6.3 / 4.3 / 4.5 / 62.3 / 24.8 / 10.8 / 0.5 / 1 / 4 / 1
Mean / 7.1 / 2.9 / 1.8 / 32.3 / 14.5 / 0.9 / 10.8 / 5.3 / 6.3 / 89.1 / 50.9 / 17.8 / 1.1 / 2.3 / 7.3 / 1
Std. Error / 2.2 / 1.1 / 0.5 / 8 / 3.7 / 0.3 / 3.1 / 1.4 / 1.8 / 23.4 / 17.9 / 7.1 / 0.4 / 0.7 / 1.7 / 0.3
Minimum / 0 / 0 / 0 / 0 / 0.5 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 32.5 / 14.5 / 6.5 / 82.5 / 49.5 / 4 / 43.5 / 20.5 / 25.5 / 318 / 246 / 117 / 5 / 7.5 / 22 / 3

Notes:

Values represent the number of calls emitted per 20-min session for each call subtype. In general, the data were positively skewed, so that mean values tended to be larger than the corresponding medians. n=16 rats, repeated measures design.

Call subtype abbreviations: CX complex, UR upward ramp, DR downward ramp, FL flat, SH short, SP split, SU step-up, SD step-down, MS multi-step, TR trill, FT flat-trill, TJ trill with jumps, IU inverted-U, CS composite, UC unclear, MI miscellaneous.

Morphine significantly increased the absolute number of trills (Z=2.67, p=0.0076) but not flat calls (p=0.3519).