Supplementary Table S1. Accession numbers for 82 NACs used for phylogenetic analysis.

S.No. / Name / Accession Number / S.No. / Name / Accession Number
1 / CaNAC1 / AY714222 / 43 / GRAB1 / AJ010829
2 / StNAC / AJ401151 / 44 / GRAB2 / AJ010830
3 / SlNAC / AY498713 / 45 / TtNAM-B1 / DQ869673
4 / PtNAM3 / AF509866 / 46 / TaNAC2 / AY625683
5 / PtNAM10 / AF509873 / 47 / AmNAC1 / EU031047
6 / ANAC / AF370617 / 48 / CyNAC1 / BAF36563
7 / CarNAC5 / FJ477886 / 49 / HvNAC6 / AM500854
8 / AtNAM / AAM51299 / 50 / HaNAC1 / AY730866
9 / ANAC032 / NP177869 / 51 / PmNAC74 / BAE48667
10 / ANAC041 / NP001118435 / 52 / TERN / AB021178
11 / XND1 / At5g64530 / 53 / SENU5 / T07182
12 / AtNAC2 / At5g39610 / 54 / AtNAM(NAP) / AAD17313
13 / AtNAC3 / At3g29035 / 55 / ANAC082 / NP196495
14 / ANAC102 / At5g63790 / 56 / ANAC103 / NP201211
15 / RD26 / At2g27410 / 57 / NAM / CAA63102
16 / Unknown / AAM15189 / 58 / CUC1 / AB049069
17 / NAC1 / AF198054 / 59 / CUC2 / AB002560
18 / NAP / At1g69490 / 60 / CUC3 / AF543194
19 / TIP / AF3281062 / 61 / ATAF1 / At1g01720
20 / NST / AAV97804 / 62 / ATAF2 / At5g08790
21 / VND6 / At5g62380 / 63 / ANAC013 (NTL1) / At1g32870
22 / VND7 / At1g71930 / 64 / ANAC014 (NTL2) / At1g33060
23 / OsNAC1 / AB028180 / 65 / ANAC016 (NTL3) / At1g34180
24 / OsNAC4 / AB028183 / 66 / ANAC053(NTL4) / At3g10500
25 / OsNAC5 / AB028184 / 67 / ANAC060 (NTL5) / At3g44290
26 / OsNAC6 / AB028185 / 68 / ANAC062 (NTL6) / At3g49530
27 / OsNAC19 / AY596808 / 69 / ANAC017 (NTL7) / At1g34190
28 / BnNAC1-1 / AY245879 / 70 / ANAC040 (NTL8) / At2g27300
29 / BnNAC5-1 / AY245881 / 71 / NTL9 / At4g35580
30 / BnNAC5-11 / AY245884 / 72 / ANAC001 (NTL10) / At1g01010
31 / BnNAC5-8 / AY245883 / 73 / ANAC078 (NTL11) / At5g04410
32 / GmNAC1 / DQ028769 / 74 / ANAC068 (NTL12) / At4g01540
33 / BnNAC14 / AY245886 / 75 / ANAC069 (NTL13) / At4g01550
34 / GmNAC3 / DQ028771 / 76 / ONAC019 (OsNTL1) / BAG89027
35 / GmNAC2 / DQ028770 / 77 / ONAC037(OsNTL2) / BAC99653
36 / GmNAC4 / DQ028772 / 78 / ONAC074(OsNTL3) / BAG95726
37 / GmNAC5 / DQ028773 / 79 / ONAC051(OsNTL3) / BAG90930
38 / GmNAC6 / DQ028774 / 80 / ONAC040(OsNTL5) / BAG94230
39 / GmNAC8 / EU661911 / 81 / ONAC070(OsNTL6) / BAG95424
40 / GmNAC15 / ACD39373 / 82 / SiNAC / GT228282
41 / GmNAC14 / EU661914
42 / GmNAC17 / EU661917

Supplementary Table S2. Summary of putative cis-acting regulatory elements identified in SiNAC promoter region.

Element / Sequence / Position from ATG (-) / Function / Reference
ABRERATCAL / CCGCGTT / 1482 / Ca2+-responsive element / Kaplan et al., 2006
ARFAT / GAGACA / 219 / Auxin response factor binding site / Ulmasov et al., 1999
CAAT-box / CAAT / 281, 479, 536, 823, 955, 1005, 1043, 1241 / Common cis-acting element in promoter and enhancer regions / Shirsat et al., 1989
CARGAT consensus / CCTATTAAGG / 366 / Cis-element found in the promoter of AtSOC1, a MADS box flowering-time gene. Flowering Locus C (FLC) protein binds to GArG box in SOC1 promoter and represses the expression of SOC1. / Hepworth et al., 2002
CATATGGMSAUR / CATATG / 1036 / Involved in auxin responsiveness / Xu et al., 1997
CBFHV / GTCGAT / 1306 / Binding site of CBF= C-repeat binding factors; known as dehydration-responsive element binding proteins (DREBs) / Xue, 2002
Circadian / CAAAGATATC / 236 / Cis-acting regulatory element involved in circadian control / Pichersky et al., 1985
DOFCORE ZM / AAAG / 235, 637, 1573 / Core site required for binding of Dof proteins in maize / Yanagisawa, 2000
DPBFCOREDCDC3 / CTTGTGT/ CCTGTGT/ / 923, 1466 / Consensus binding site of the LEA-gene Dc3 promoter binding factor DPBF, a bZIP protein from sun flower / Kim et al.,1997
ERF1 / GCCGCC / 1408 / Ethylene-responsive element / Brown et al.,
2003
GATA-box / GATA / 322, 382, 585, 870, 898, 907, 1000 / Required for high level, light regulated, and tissue specific expression / Gilmartin et al.,1990
G-box / GACATGTGGT / Cis-acting regulatory element involved in light responsiveness / Viret et al., 1994
MBS / CGGTCA / 1120 / MYB Binding Site / Nash et al., 1990
MYBCORE ATCYCB1 / CCGTT / 935 / Activates reporter gene without leading to M-phase-specific expression / Planchais et al.,
2002
MYCCONSENSUS AT / CANNTG
(N=A/T/G/C) / 244, 373, 613, 679 , 1036, 1421, 1468 / Dehydration-responsive element / Abe et al.,
2003
PREATPRODH / ACTCAT / 709 / Pro- or hypoosmolarity-responsive element / Satoh et al., 2002
rbcS consensus / TTGGATT / 458 / Influences the level of gene expression and involved in
light regulated gene expression / Manzara et al.,
1988
RHERPATEXPA7 / TCGTGA / 409 / Root Hair-specific Cis-Elements / Kim et al., 2006
SEBFCONSSTPR10A / YTGTCWC
(W=A/T) / 166, 714 / Binding site of the potato silencing element binding factor(SEBF) gene found in promoter of pathogenesis-related gene (PR-10a) / Boyle and Brisson, 2001
SEF4-motif GM7S / RTTTTTR (R=A/G) / 1250 / Soybean embryo factor 4 binding site / Allen et al.,
1989
Skn1-motif / GTCAT / 1038, 1118 / Cis-acting regulatory element required for endosperm expression / Takaiwa et al., 1991
TATABOX4 / TATATAA / 1519 / Core promoter element around -30 of transcription start / Grace et al., 2004
TATABOX5 / TTATTT / 820 / TATA box found in the 5'upstream region of pea glutamine synthetase gene / Tjaden et al., 1995
TGACG-motif / TGACG / 1417 / Cis-acting regulatory element involved in the MeJA-responsiveness / Rouster et al., 1997
W-box ATNPR1 / GTCAA / 509 / W-box found in promoter of Arabidopsis thaliana NPR1 gene; They were recognized specifically by salicylic acid-induced WRKY DNA binding proteins / Yu et al., 2001
W-box NTERF3 / GGTCA / 875, 1119 / W box found in the promoter region of a transcriptional repressor ERF3 gene; May be involved in activation of ERF3 gene by wounding / Nishiuchi et al., 2004
WRKY71OS / GTCA / 65, 509, 873, 1038, 1118 / A core of TGAC-containing W-box; Binding site of rice WRKY71, a transcriptional repressor of the gibberellin signaling pathway / Zhang et al., 2004

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