Supplementary Table 2: Examples of pre-clinical studies testing therapeutic engineered bacteria in animal disease models. I (exp) = experimentally inducible promoter; I (qs) = quorum sensing inducible promoter; I(phys) = physiological inducible promoter; C = constitutive promoter; P = plasmid based; Ch = chromosomally integrated; O = oral delivery; IV = intravenous delivery, IP = intraperitoneal injection; T = intratumoural injection; V = intravaginal delivery; R = rectal delivery; T = topical administration.

Disease / Therapy / Bacteria / Constitutive/ Inducible Expression / Plasmid/ Chromosome / Delivery method / Test system / Citation
Cancer / IL-2 / S. Typhimurium / C / P / O / Mouse: hepatic metastasis model / 30
Cancer / Cytosine deaminase (to enzymatically convert 5-fluorocytosine to the anticancer drug 5-fluorouracil) / S. Typhimurium / C / P / IV / Mouse: subcutaneously implanted murine tumors / 31
Cancer / HPV-16 E7 protein / L. monocytogenes / C / C / IP / Mouse: subcutaneously implanted murine tumors / 32
Cancer / Cytosine deaminase (to enzymatically convert 5-fluorocytosine to the anticancer drug 5-fluorouracil) / C. sporogenes / C / P / IV / Mouse: subcutaneously implanted murine tumors / 33
Cancer / NY-ESO-1 / S. Typhimurium / C / P / O/ T / Mouse: human melanoma xenografts / 34
Cancer / Invasin; listeriolysin O; α- catenin β1 shRNA. / E. coli BL21DE3 / C / P / O / IV / Mouse: human colon cancer xenografts / 29
Cancer / LIGHT cytokine / S. Typhimurium / C / P / IV / Mouse: breast and colon carcinoma xenografts / 35
Cancer / Hemolysin E / S. Typhimurium / I (phys) / P / IV / Mouse: subcutaneous implanted murine tumors / 36
Cancer / PhageX174E lysin; hemolysin E/ CCL21/ Bit-iGRD peptide / S. Typhimurium / I (qs) / P / T / Mouse: subcutaneous colorectal cancer and hepatic metastasis models / 37
Cancer / Flagellin B / S. Typhimurium / I (exp) / P / IV / Mouse: mouse and human colon cancer xenograft / 7
Colitis/ IBD / mIL-10 / L. lactis / C / P / O / Mouse: DSS and IL10-/- / 11
Colitis/ IBD / hIL-10 / L. lactis / C / Ch / O / Pig: biocontainment testing / 38
Colitis/ IBD / hIL-10 / L. lactis / C / Ch / O / Mouse: DSS and IL10-/- / 39
Colitis/ IBD / IL-1Ra / S. gordonii / C / P / V/ O / Mouse: vaginal and gastrointestinal delivery; IL2-/- / 40
Colitis/ IBD / IL-1Ra/ IL-1β / B. subtilis / C (autolysis in vivo) / P / R / IP (into intestine) / Mouse/ Rat/ Rabbit: local and systemic delivery; Rabbit: IL-1 protection/ IL-1β induced shock; Mouse: LPS-induced shock; / 41
Colitis/ IBD / Trefoil factor-1, 2 and 3 / L. lactis / C / P / O / Mouse: DSS and IL10-/- / 13
Colitis/ IBD / hTGFβ -1 / B. ovatus / I (exp) / Ch / O / Mouse: DSS / 42
Colitis/ IBD / Elafin / L. lactis; L. casei / I (qs) / P / O / Mouse: DSS / 12
Colitis/ IBD / IL-27 / L. lactis / C / P / O / Mouse: DSS and Rag1-/- T cell transfer colitis / 8
Colitis/ IBD / Cystatin / E. coli Nissle 1917 / C / P / O / Mouse: DSS; Pig: postweaning inflammation model / 43
Diabetes (T1) / GLP-1 (1-37) / L. gasseri / C / Ch / O / Rat: streptozotocin induced diabetes model / 46
Diabetes (T1) / hIL10; proinsulin / L. lactis / C / P / O / Mouse: NOD diabetes model / 47
Diabetes (T1) / hIL10; proinsulin / L. lactis / C / Ch / O / Mouse: NOD diabetes model / 4
Diabetes (T1) / hIL-10; GAD-65 / L. lactis / C / P / O / Mouse: NOD diabetes model / 48
Diabetes (T2) / GLP-1 / L. lactis / C / P / O / Rat: ZDF diabetes model / 49
Diabetes (T2) / GLP-1 / L. paracasei / C / P / O / Rat: GK diabetes model / 50
HIV / HIV-gp41-hemolysin A / E. coli Nissle 1917 / C / P / O/ R / Mouse: colonization and delivery testing / 54
HIV / Cyanovirin-N / L. jensenii / C / Ch / V / Mouse: colonization and delivery testing / 55
HIV / Cyanovirin-N / L. jensenii / C / Ch / V / Macaque monkey: SHIV infection model / 6
Infection / Shiga toxin receptor mimetic / E. coli R1 / C / P / O / Mouse: Shiga toxigenic E. coli infection model / 51
Infection / GM1 ganglioside / E. coli R1 / C / P / O / Mouse: Infant cholera model / 52
Infection / CAI quorum sensing / E. coli Nissle 1917 / C / P / O / Mouse: infant cholera model / 53
Infection / Microcin J25 / E. coli Nissle 1917 / C / P / O / Turkey: S. Typhimuriuminfection model / 10
Infection / Lysin E7; pyosin S5 and dispersin-B / E. coli Nissle 1917 / I (qs) / P / O / C. elegans/ Mouse: P. aeruginosa gut infection model / 9
Oral mucositis / Trefoil factor 1 / L. lactis / C / Ch / T / Hamster: Radiation induced mucositis / 44
Dental Carries / S. mutans antigen I/II scFv / L. zeae / C / P / T / Rat: dental caries model / 45
Obesity / N-acylphosphatidylethanolamines / E. coli Nissle 1917 / C / P / O / Mouse: high-fat diet, TallyHo obesity models / 56
Hypertension / Angiotensin-converting enzyme inhibitory peptides / L. plantarum / I (exp) / P / O / Rat: Spontaneous hypertension model / 58