Supplementary Table 1A. Polymerase chain reaction primers sequences
Gene / Forward sequence (5’-3’) / Reverse sequence (5’-3’) / Size (bp)ACTB / ACGGCATCGTCACCAACT / AGGAAGGAAGGCTGGAAGAG / 583
NANOG / AGAAGGCCTCAGCACCTAC / GGCCTGATTGTTCCAGGATT / 205
Supplementary Table 1B. Real time PCR primers sequences
Gene / Forward sequence (5’-3’) / Reverse sequence (5’-3’) / Size(bp)
18S / GTTCCGACCATAAACGATGC / AACCAGACAAATCGCTCCAC / 298
AFP / TGCAGCCAAAGTGAAGAGGGAA / ATAGCGAGCAGCCCAAAGAAGA / 215
Albumin / CTTCCTGGGCATGTTTTTGT / TGGCATAGCATTCATGAGGA / 140
B2M / CATCCAGCGTACTCCAAAGA / GACAAGTCTGAATGCTCCAC / 165
CDX2 / GGGCTCTCTGAGAGGCAGGT / CCTTTGCTCTGCGGTTCTG / 52
CXCR4 / CACCGCATCTGGAGAACCA / GCCCATTTCCTCGGTGTAGTT / 79
FLK1 / ACTTTGGAAGACAGAACCAAAT
TATCTC / TGGGCACCATTCCACCA / 51
FOXA2 / GGGAGCGGTGAAGATGGA / TCATGTTGCTCACGGAGGAGTA / 89
FOXF1 / GCCGAGCTGCAAGGCA / AACTCCTTTCGGTCACACATGC / 64
GAPDH / GTCAGTGGTGGACCTGACCT / CCCTGTTGCTGTAGCCAAAT / 250
GATA4 / CATCAAGACGGAGCCTGGCC / TGACTGTCGGCCAAGACCAG / 218
GATA6 / CCATGACTCCAACTTCCACC / ACGGAGGACGTGACTTCGGC / 214
HLXB9 / CACCGCGGGCATGATC / ACTTCCCCAGGAGGTTCGA / 71
HNF1-b / TCACAGATACCAGCAGCATCAGT / GGGCATCACCAGGCTTGTA / 79
HNF6 / CGCTCCGCTTAGCAGCAT / GTGTTGCCTCTATCCTTCCCAT / 61
Insulin / AAGAGGCCATCAAGCAGATCA / CAGGAGGCGCATCCACA / 58
Laminin B1 / ACTGAAGAGTCAGCTGATGC / AGTGAACGGACTTCTCCTTC / 187
NANOG / AGAAGGCCTCAGCACCTAC / GGCCTGATTGTTCCAGGATT / 205
NGN3 / GCTCATCGCTCTCTATTCTTTTGC / GGTTGAGGCGTCATCCTTTCT / 51
OCT4 / TGGGCTCGAGAAGGATGTG / GCATAGTCGCTGCTTGATCG / 58
PDX1 / AAGTCTACCAAAGCTCACGCG / GTAGGCGCCGCCTGC / 51
SOX7 / ACGCCGAGCTCAGCAAGAT / TCCACGTACGGCCTCTTCTG / 73
SOX17 / GGCGCAGCAGAATCCAGA / CCACGACTTGCCCAGCAT / 61
ZIC1 / CTGGCTGTGGCAAGGTCTTC / CAGCCCTCAAACTCGCACTT / 96