Supplementary Table 1. Worm Strains Used in This Study

Cheeseman et al., p.1

Supplementary Material

Supplementary Table 1. Worm Strains used in this study.

Supplementary Table 2. dsRNAs used in this study.

• dsRNAs were amplified from either N2 genomic DNA (61, 84, 93, and 94) or gene specific cDNAs (171, 199-204).

Supplementary Table 3. Summary of supplemental movies.

• In all videos, the anterior of the embryo is to the left and the posterior of the embryo is to the right.

• All images were acquired using widefield deconvolution microscopy

• For TH32 (GFP-Histone H2B + GFP--tubulin), one 3D z-stack (6 images at 2 µm steps) was acquired every 10s

• For GFP-kinetochore protein strains, one 3D z-stack (6 images at 1 µm steps) was acquired every 10s

• Maximal intensity projections of z-stacks were used to generate each movie frame

• All images from the same strain were scaled with respect to the wild type control

• Video playback is at 10 frames/sec

Video #