Supplementary Table 1. Wolbachia strains and closely related bacterial species used in this work

Bacterium (straina) / Wolbachia host / Wolbachia supergroup / Number of binding sites
DnaA / CtrA / IHF
wAspaA / Acromis sparsa / A / 3 / 5 / 1
wAlbA / Aedes albopictus / A / 3 / 6 / 1
wCpenA / Camponotus pennsylvanicus / A / 3 / 5 / 1
wAna / Drosophila ananassae / A / 3 / 4 / 1
wDbifA / Drosophila bifasciata / A / 3 / 4 / 1
wDin / Drosophila innubila / A / 3 / 4 / 1
wMel / Drosophila melanogaster / A / 3 / 4 / 1
wMelPop / Drosophila melanogaster / A / 3 / 4 / 1
wDneoA / Drosophila neotestacea / A / 3 / 4 / 1
wDoriA / Drosophila orientacea / A / 3 / 4 / 1
wDrecA / Drosophila recens / A / 3 / 4 / 1
wSan / Drosophila santomea / A / 3 / 4 / 1
wAu / Drosophila simulans / A / 3 / 4 / 1
wRi / Drosophila simulans / A / 3 / 4 / 1
wHa / Drosophila simulans / A / 3 / 4 / 1
wSim / Drosophila simulans / A / 3 / 4 / 1
wTei / Drosophila teissieri / A / 3 / 4 / 1
wWil / Drosophila willistoni / A / 3 / 4 / 1
wYak / Drosophila yakuba / A / 3 / 4 / 1
wKueA / Ephestia kuehniella / A / 3 / 4 / 1
wMors / Glossina morsitans / A / 3 / 4 / 1
wUni / Muscidifurax uniraptor / A / 3 / 4 / 1
wNgir / Nasonia giraulti / A / 3 / 4 / 1
wNlon / Nasonia longicornis / A / 3 / 4 / 1
wNvitA / Nasonia vitripennis / A / 3 / 4 / 1
wPhofA / Pegoscapus hoffmeyeri / A / 3 / 3 / 1
wSinvictaA / Solenopsis invicta / A / 3 / 5 / 1
wAencB / Acraea encedon / B / 3 / 4 / 1
wAepoB / Acraea eponina / B / 3 / 6 / 1
wAsocconB / Allonemobius socius / B / 3 / 5 / 1
wAsocB / Allonemobius socius / B / 3 / 5 / 1
wVul / Armadillidium vulgare / B / 3 / 7 / 1
wCalt / Chelymorpha alternans / B / 3 / 5 / 1
wPip / Culex pipiens / B / 3 / 6 / 1
wPip / Culex pipiens quinquefasciatus / B / 3 / 6 / 1
wMau / Drosophila mauritiana / B / 3 / 5 / 1
wMa / Drosophila simulans / B / 3 / 5 / 1
wNo / Drosophila simulans / B / 3 / 5 / 1
wFor / Encarsia formosa / B / 3 / 6 / 1
wKueB / Ephestia kuehniella / B / 3 / 6 / 1
wGfirB / Gryllus firmus / B / 3 / 5 / 1
wNvitB / Nasonia vitripennis / B / 3 / 5 / 1
wOscaB / Ostrinia scapulalis / B / 3 / 5 / 1
wProtB / Protocalliphora sialia 00172 / B / 3 / 6 / 1
wCon / Tribolium confusum / B / 3 / 6 / 1
wDei / Trichogramma deion / B / 3 / 5 / 1
wKayB / Trichogramma kaykai / B / 3 / 5 / 1
wTaiB / Teleogryllus taiwanemma / B / 3 / 5 / 1
wUrtB / Tetranychus urticae / B / 3 / 6 / 1
wBm / Brugia malayi / D / 3 / 2 / 1
wFcanE / Folsomia candida / E / 3 / 5 / 1
wClecF / Cimex lectularius / F / 3 / 2 / 1
wCsco / Cordylochernes scorpioides / No grouping / 3 / 0 / 1
Anaplasma marginale St. Maries / N/A / N/A / 2 / 1b / 1
Anaplasma phagocytophilum HZ / N/A / N/A / 4 / 1 / 1c
Ehrlichia ruminantium Welgevondend / N/A / N/A / 2 / 0e / 2
Ehrlichia canisJake / N/A / N/A / 4 / 3 / 2
Ehrlichia chaffensisArkansas / N/A / N/A / 4 / 1 / 1
Rickettsia prowazekiiMadrid E / N/A / N/A / 4 / 6 / 1
Rickettsia conoriiMalish 7 / N/A / N/A / 3 / 2 / 1c
Rickettsia typhiWilmington / N/A / N/A / 4 / 5 / 1
Rickettsia felisURRWXCal2 / N/A / N/A / 3 / 3 / 1
Neorickettsia sennetsuMiyayama / N/A / N/A / 0 / 0 / 0
N/A / N/A / 0 / 0 / 2
CaulobactercrescentusCB15f / N/A / N/A / 5 / 5 / 1

N/A: not applicable.

CtrA binding site consensus: TTAA-N7-TTAA with one mismatch in the A’s

DnaA box consensus: TTAΤNCACA

IHF binding site consensus: WATCAN5WTR

a The first 53 strains are found in Wolbachia.

b The single CtrA binding site of A. marginale is located 150 nucleotides downstream COG1253’s start codon

c IHF binding site falls inside hemE (for A. phagocytophilum) or inside COG1806 (for R. conorii).

dE. ruminantium Welgevonden is identical to E. ruminantium Gardel and is not shown separately in this table.

etwo CtrA boxes can be found if two mismatches are permitted.

f Protein binding sites of C. crescentusori region were taken from the manuscript which characterized it experimentally (Brassinga et al. 2002). These binding sites are not necessarily located between hemE and COG1806 genes.