Supplementary Table 1 T-Test for the Protein Spot Accumulation Differences Between Wheat

Supplementary Table 1 t-test for the protein spot accumulation differences between wheat hybrid and parents

Spot 105 (LDH)

t-Test: Two-Sample Assuming Equal Variances / t-Test: Two-Sample Assuming Equal Variances
3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.183356 / 0.015447 / Mean / 0.061259 / 0.015447
Variance / 0.002278 / 1.33E-05 / Variance / 0.001284 / 1.33E-05
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.001145 / Pooled Variance / 0.000649
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 6.076093 / t Stat / 2.202768
P(T<=t) one-tail / 0.001854 / P(T<=t) one-tail / 0.046184
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.003707 / P(T<=t) two-tail / 0.092367
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 205 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.402902 / 0.075407 / Mean / 0.114266 / 0.075407
Variance / 0.012339 / 0.000205 / Variance / 0.000847 / 0.000205
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.006272 / Pooled Variance / 0.000526
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 5.064453 / t Stat / 2.074991
P(T<=t) one-tail / 0.003579 / P(T<=t) one-tail / 0.053313
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.007158 / P(T<=t) two-tail / 0.106626
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 222 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 1.042339 / 0.429739 / Mean / 0.337907 / 0.429739
Variance / 0.016136 / 0.005515 / Variance / 0.002196 / 0.005515
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.010826 / Pooled Variance / 0.003856
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 7.210979 / t Stat / -1.81129
P(T<=t) one-tail / 0.00098 / P(T<=t) one-tail / 0.072165
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.001961 / P(T<=t) two-tail / 0.14433
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 223 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 1.05094 / 0.40952 / Mean / 0.386276 / 0.40952
Variance / 0.101573 / 0.000137 / Variance / 0.001506 / 0.000137
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.050855 / Pooled Variance / 0.000821
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 3.483553 / t Stat / -0.99344
P(T<=t) one-tail / 0.012635 / P(T<=t) one-tail / 0.188363
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.02527 / P(T<=t) two-tail / 0.376726
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 807 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.20838 / 0.219262 / Mean / 0.431339 / 0.219262
Variance / 0.001796 / 0.000187 / Variance / 0.000459 / 0.000187
Observations / 3 / 2 / Observations / 3 / 2
Pooled Variance / 0.001259 / Pooled Variance / 0.000368
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 3 / df / 3
t Stat / -0.3359 / t Stat / 12.10412
P(T<=t) one-tail / 0.379532 / P(T<=t) one-tail / 0.000607
t Critical one-tail / 2.353363 / t Critical one-tail / 2.353363
P(T<=t) two-tail / 0.759064 / P(T<=t) two-tail / 0.001214
t Critical two-tail / 3.182449 / t Critical two-tail / 3.182449

Spot 865 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.37035 / 0.228839 / Mean / 0.143744 / 0.228839
Variance / 0.060788 / 0.002453 / Variance / 5.11E-05 / 0.002453
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.03162 / Pooled Variance / 0.001252
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 0.974658 / t Stat / -2.94563
P(T<=t) one-tail / 0.19246 / P(T<=t) one-tail / 0.021076
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.384919 / P(T<=t) two-tail / 0.042152
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 869 (UPF1)

3338 / 3338/2463
Mean / 0.083875 / 0.050382
Variance / 0.00064 / 0.000946
Observations / 3 / 3
Pooled Variance / 0.000793
Hypothesized Mean / 0
df / 4
t Stat / 1.456843
P(T<=t) one-tail / 0.109442
t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.218885
t Critical two-tail / 2.776451

Spot 931 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.076353 / 0.048474 / Mean / 0.110911 / 0.048474
Variance / 0.000329 / 0.000298 / Variance / 0.000357 / 0.000298
Observations / 3 / 2 / Observations / 3 / 2
Pooled Variance / 0.000319 / Pooled Variance / 0.000337
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 3 / df / 3
t Stat / 1.710625 / t Stat / 3.725105
P(T<=t) one-tail / 0.092838 / P(T<=t) one-tail / 0.016844
t Critical one-tail / 2.353363 / t Critical one-tail / 2.353363
P(T<=t) two-tail / 0.185677 / P(T<=t) two-tail / 0.033688
t Critical two-tail / 3.182449 / t Critical two-tail / 3.182449

Spot 937 (DRH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.634589 / 0.335351 / Mean / 0.762364 / 0.335351
Variance / 0.007878 / 7.02E-05 / Variance / 0.017631 / 7.02E-05
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.003974 / Pooled Variance / 0.008851
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 5.813496 / t Stat / 5.559044
P(T<=t) one-tail / 0.002179 / P(T<=t) one-tail / 0.002563
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.004358 / P(T<=t) two-tail / 0.005127
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 981 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.474094 / 0.291747 / Mean / 0.303632 / 0.291747
Variance / 0.002936 / 0.001864 / Variance / 0.000233 / 0.001864
Observations / 2 / 3 / Observations / 3 / 3
Pooled Variance / 0.002221 / Pooled Variance / 0.001049
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 3 / df / 4
t Stat / 4.238127 / t Stat / 0.449539
P(T<=t) one-tail / 0.012025 / P(T<=t) one-tail / 0.338163
t Critical one-tail / 2.353363 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.024049 / P(T<=t) two-tail / 0.676326
t Critical two-tail / 3.182449 / t Critical two-tail / 2.776451

Spot 991 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.204016 / 0.333751 / Mean / 0.226112 / 0.333751
Variance / 0.000172 / 0.002318 / Variance / 0.017145 / 0.002318
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.001245 / Pooled Variance / 0.009731
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -4.50291 / t Stat / -1.33637
P(T<=t) one-tail / 0.005399 / P(T<=t) one-tail / 0.12619
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.010798 / P(T<=t) two-tail / 0.25238
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1017 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.11293 / 0.061331 / Mean / 0.091222 / 0.061331
Variance / 0.001451 / 7.31E-05 / Variance / 0.000123 / 7.31E-05
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000762 / Pooled Variance / 9.82E-05
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 2.289658 / t Stat / 3.693825
P(T<=t) one-tail / 0.041944 / P(T<=t) one-tail / 0.010474
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.083889 / P(T<=t) two-tail / 0.020948
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1050 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.264618 / 0.372859 / Mean / 0.336018 / 0.372859
Variance / 0.000998 / 0.00088 / Variance / 5.09E-05 / 0.00088
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000939 / Pooled Variance / 0.000465
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -4.32624 / t Stat / -2.09147
P(T<=t) one-tail / 0.006193 / P(T<=t) one-tail / 0.052328
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.012386 / P(T<=t) two-tail / 0.104657
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1220 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.286806 / 0.434717 / Mean / 0.44589 / 0.434717
Variance / 3.36E-05 / 2.59E-09 / Variance / 0.01653 / 2.59E-09
Observations / 3 / 2 / Observations / 3 / 2
Pooled Variance / 2.24E-05 / Pooled Variance / 0.01102
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 3 / df / 3
t Stat / -34.2086 / t Stat / 0.116591
P(T<=t) one-tail / 2.75E-05 / P(T<=t) one-tail / 0.457276
t Critical one-tail / 2.353363 / t Critical one-tail / 2.353363
P(T<=t) two-tail / 5.49E-05 / P(T<=t) two-tail / 0.914551
t Critical two-tail / 3.182449 / t Critical two-tail / 3.182449

Spot 1060 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.079857 / 0.171733 / Mean / 0.130279 / 0.171733
Variance / 0.00069 / 0.000775 / Variance / 0.000276 / 0.000775
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000732 / Pooled Variance / 0.000525
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -4.15834 / t Stat / -2.21492
P(T<=t) one-tail / 0.007082 / P(T<=t) one-tail / 0.045563
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.014164 / P(T<=t) two-tail / 0.091126
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1070 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.146718 / 0.115326 / Mean / 0.243416 / 0.115326
Variance / 0.000452 / 0.000566 / Variance / 0.000109 / 0.000566
Observations / 3 / 3 / Observations / 4 / 3
Pooled Variance / 0.000509 / Pooled Variance / 0.000292
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 5
t Stat / 1.704266 / t Stat / 9.814153
P(T<=t) one-tail / 0.081768 / P(T<=t) one-tail / 9.35E-05
t Critical one-tail / 2.131846 / t Critical one-tail / 2.015049
P(T<=t) two-tail / 0.163535 / P(T<=t) two-tail / 0.000187
t Critical two-tail / 2.776451 / t Critical two-tail / 2.570578

Spot 1076 (URH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.091766 / 0.149581 / Mean / 0.087272 / 0.149581
Variance / 0.000792 / 3.23E-05 / Variance / 7.78E-05 / 3.23E-05
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000412 / Pooled Variance / 5.51E-05
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -3.48836 / t Stat / -10.2849
P(T<=t) one-tail / 0.01258 / P(T<=t) one-tail / 0.000252
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.02516 / P(T<=t) two-tail / 0.000504
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1078 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.341335 / 0.315404 / Mean / 0.481408 / 0.315404
Variance / 0.010107 / 0.003145 / Variance / 0.000644 / 0.003145
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.006626 / Pooled Variance / 0.001895
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 0.390157 / t Stat / 4.670596
P(T<=t) one-tail / 0.358153 / P(T<=t) one-tail / 0.004757
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.716306 / P(T<=t) two-tail / 0.009514
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1093 (DRH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.112197 / 0.052565 / Mean / 0.230305 / 0.052565
Variance / 0.00115 / 4.53E-05 / Variance / 8.78E-05 / 4.53E-05
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000597 / Pooled Variance / 6.65E-05
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 2.987858 / t Stat / 26.68642
P(T<=t) one-tail / 0.020212 / P(T<=t) one-tail / 5.86E-06
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.040424 / P(T<=t) two-tail / 1.17E-05
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 202 (LDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.898688 / 0.132767 / Mean / 0.235516 / 0.132767
Variance / 0.090099 / 0.001878 / Variance / 0.003514 / 0.001878
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.045988 / Pooled Variance / 0.002696
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 4.374268 / t Stat / 2.423793
P(T<=t) one-tail / 0.005964 / P(T<=t) one-tail / 0.036233
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.011928 / P(T<=t) two-tail / 0.072465
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1235 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.197866 / 0.386628 / Mean / 0.330786 / 0.386628
Variance / 0.0001 / 0.01256 / Variance / 0.001418 / 0.01256
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.00633 / Pooled Variance / 0.006989
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -2.90568 / t Stat / -0.81807
P(T<=t) one-tail / 0.021934 / P(T<=t) one-tail / 0.229624
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.043867 / P(T<=t) two-tail / 0.459248
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1244 (UPF1)

3338 / 3338/2463
Mean / 0.062263 / 0.154644
Variance / 0.000777 / 0.004282
Observations / 3 / 3
Pooled Variance / 0.00253
Hypothesized Mean / 0
df / 4
t Stat / -2.24946
P(T<=t) one-tail / 0.043849
t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.087697
t Critical two-tail / 2.776451

Spot 1251 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.111369 / 0.170984 / Mean / 0.185264 / 0.170984
Variance / 0.000214 / 0.000993 / Variance / 0.001197 / 0.000993
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000604 / Pooled Variance / 0.001095
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -2.97133 / t Stat / 0.528537
P(T<=t) one-tail / 0.020545 / P(T<=t) one-tail / 0.312547
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.04109 / P(T<=t) two-tail / 0.625093
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1267 (UPF1)

3338 / 3338/2463
Mean / 0.034237 / 0.059879
Variance / 2.76E-05 / 0.000236
Observations / 3 / 3
Pooled Variance / 0.000132
Hypothesized Mean / 0
df / 4
t Stat / -2.73792
P(T<=t) one-tail / 0.026009
t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.052017
t Critical two-tail / 2.776451

Spot 1269 (URH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.061333 / 0.220019 / Mean / 0.048666 / 0.220019
Variance / 0.00036 / 0.007668 / Variance / 0.000156 / 0.007668
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.004014 / Pooled Variance / 0.003912
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -3.06759 / t Stat / -3.35532
P(T<=t) one-tail / 0.01869 / P(T<=t) one-tail / 0.014214
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.037381 / P(T<=t) two-tail / 0.028428
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1275 (HDH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.074573 / 0.116187 / Mean / 0.141332 / 0.116187
Variance / 0.000258 / 0.000351 / Variance / 8.11E-06 / 0.000351
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000305 / Pooled Variance / 0.00018
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / -2.91976 / t Stat / 2.297053
P(T<=t) one-tail / 0.021627 / P(T<=t) one-tail / 0.041604
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.043254 / P(T<=t) two-tail / 0.083208
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1095 (DRH)

3338 / 3338/2463 / 2463 / 3338/2463
Mean / 0.105925 / 0.058936 / Mean / 0.198331 / 0.058936
Variance / 8.67E-05 / 0.00059 / Variance / 0.002748 / 0.00059
Observations / 3 / 3 / Observations / 3 / 3
Pooled Variance / 0.000338 / Pooled Variance / 0.001669
Hypothesized Mean / 0 / Hypothesized Mean / 0
df / 4 / df / 4
t Stat / 3.129169 / t Stat / 4.179305
P(T<=t) one-tail / 0.017607 / P(T<=t) one-tail / 0.006963
t Critical one-tail / 2.131846 / t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.035213 / P(T<=t) two-tail / 0.013926
t Critical two-tail / 2.776451 / t Critical two-tail / 2.776451

Spot 1322-1 (UPF1)

2463 / 3338/2463
Mean / 0.26197 / 0.084704
Variance / 0.027407 / 0.004676
Observations / 3 / 3
Pooled Variance / 0.016042
Hypothesized Mean Difference / 0
df / 4
t Stat / 1.714146
P(T<=t) one-tail / 0.080827
t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.161654
t Critical two-tail / 2.776451

Spot 1322-2 (UPF1)

3338 / 3338/2463
Mean / 0.198937 / 0.055981
Variance / 0.003065 / 0.000277
Observations / 3 / 3
Pooled Variance / 0.001671
Hypothesized Mean Difference / 0
df / 4
t Stat / 4.282952
P(T<=t) one-tail / 0.006409
t Critical one-tail / 2.131846
P(T<=t) two-tail / 0.012818
t Critical two-tail / 2.776451