Supplementary Table 1 Marker name, forward and reverse primer, PCR program for each marker
Marker / Forward / Reverse / PCR program a / Tm used in PCRafct45 / TAAAAAACGGAAAGAGTTGGTTAG / GCCATCTTTTCTTTTGCTTC / A / 55
maa660456 / GGGTTTTTGATCCAGATCTT / AAGGTGGTCATACGAGCTCC / A / 55
maa660870 / GTACATCAACAACTTTCTCCT / ATCAACAAAATTCATCGAAC / A / 55
b14b03 / GCTTGTTCTTCTTCAAGCTC / ACCTGACTTGTGTTTTATGC / A / 55
b21e13 / GCCGATGGTACTAATGTAGG / AAATCTTGCTTGCTTCTCAG / A / 55
mtic19 / TCTAGAAAAAGCAATGATGTGAGA / TGCAACAGAAGAAGCAAAACA / A / 55
mtic93 / AGCAGGATTTGGGACAGTTG / TACCGTAGCTCCCTTTTCCA / A / 55
mtic94 / GCTACAACAGCGCTACATCG / CAGGGTCAGAGCAACAATCA / A / 55
mtic95 / AAAGGTGTTGGGTTTTGTGG / AGGAAGGAGAGGGACGAAAG / A / 55
mtic103 / TGGGTTGTCCTTCTTTTTGG / GGGTGCAGAAGTTTGACCA / A / 55
mtic124 / TTGTCACGAGTGTTGGAATTTT / TTGGGTTGTCAATAATGCTCA / A / 55
mtic134 / GCAGTTCGCTGAGGACTTG / CAATTAGAGTCTACAGCCAAAAACT / A / 55
mtic188 / GGCGGTGAAGAAGTAAACGA / AATCGGAGAAACACGAGCAC / A / 55
mtic189 / CAAACCCTTTTCAATTTCAACC / ATGTTGGTGGATCCTTCTGC / A / 55
mtic210 / CCAAACTGGCTGTGTTCAAA / GCGGTAAGCCTTGCTGTATG / A / 55
mtic237 / CCCATATGCAACAGACCTTA / TGGTGAAGATTCTGTTGTTG / A / 55
mtic238 / TTCTTCTTCTAGGAATTTGGAG / CCTTAGCCAAGCAAGTAAAA / A / 55
mtic331 / CCCTCTTCTACCTCCTTTCCA / GGAAGAGAAGATGGGGGTGT / A / 55
mtic332 / CCCTGGGTTTTTGATCCAG / GGTCATACGAGCTCCTCCAT / A / 55
mtic343 / TCCGATCTTGCGTCCTAACT / CCATTGCGGTGGCTACTCT / A / 55
mtic345 / TCCGATCTTGCGTCCTAACT / CCATTGCGGTGGCTACTCT / A / 55
mtic354 / AAGTGCCAAAGAACAGGGTTT / AACCTACGCTAGGGTTGCAG / A / 55
mtic451 / GGACAAAATTGGAAGAAAAA / AATTACGTTTGTTTGGATGC / A / 55
mtic452 / CTAGTGCCAACACAAAAACA / TCACAAAAACTGCATAAAGC / A / 55
mtic470 / GGTTCGTGTATTTGTTCGAT / CCCTTCACAGAATGATTGAT / A / 55
aw220 / GCCACAATTTTCTCATCATCAC / TGCTGCTGTGCCGTAGTAGATA / A / 55
aw242 / CGTCACACCAACTTTATCACCA / TTCTCCAAGAGGAGCTTAACCA / A / 55
aw254 / TATATGCTTGTTGAGGCCACTG / CACATCTTCGTCATCATCTTCA / A / 55
aw281 / TCACCAATCCATCAAACTTCAC / AGCTTCAGTGTCATCTCGTGTC / A / 55
aw282 / CGACCAAATCACTCTTCTTCAA / AATCCAAGACCATTCACCTGAG / A / 55
aw290/aw332 / TGAGAGATTGATGGGCAATACA / AAGTTGAAGGAAGGTGGTGGT / A / 55
aw295 / CAACATTCTTCCATTTCCTTCC / TCTTCATCTTCGTCGTCTTCAA / A / 55
aw312 / CTGTGGGGAACAAGAAGAAGAG / CCAGTAACAACAGTCCCATTTG / A / 55
aw317 / ACGCACATTTCCATTCTCATTC / TTTTCGATTAGGTCGTGGATCT / A / 55
aw343 / GGTTCGTGTATTTGTTCGATCC / AATCTCCAAGGTTCCATCTTCA / A / 55
aw350 / GAACCTCTTCTTCTCTCACACAAA / AGCATCGAACGGATGTAATTG / A / 55
aw369 / GCGCTCATCATCTTCATCTAAA / AGAATTGAGACATGGCAGAGG / A / 55
aw394 / AGGATGATGTGGAAGGAAGAAA / TTGCTAGAGCCTTAAACCCTGT / A / 55
be100 / GCATTAGCACCCTCATTCATATC / TGCAGAGACTTTTGAACACCTT / A / 55
be103 / AATGGCGAACACTTTCACTCTT / GATGGTTTCTTCGAGACGAGAG / A / 55
be114 / CCACCTCATCACTCCGTAAAA / ATGAAGCTGTTGTTGTTGCAGT / A / 55
be322616 / GCTAGTTCTGCTCTCACTCTCATC / CATTGTCTTTGTTGTGGAGGTG / A / 55
be123 / ATCACAAGCCTCAACAGCCATA / TTGATGGGTAAAGGAGAAGGTG / A / 55
be325253 / GAAGTTGCGTCAGAGAGATCAG / AACAAACACAGGCTTCACCATA / A / 55
be149 / GTGTTTGGGAGATTTTGAGGAG / GCATGATAGCAAGTGGAACCATA / A / 55
be68 / TCTGTTTACCACACGCAACTTC / CAGAAGCCATTAGCCTGAACAT / A / 55
be203866 / TACTGTCCCAATCTTCACAACG / GCACAAGCAGCCATATTGATAG / A / 55
be81 / CGCTCTTGTTGATTCTGCTATG / TTACTCTTCTCCTTGGCAGCTT / A / 55
be84 / TCCGAACCCTACTTCCAAATTA / TGGGATACTGATTTTCTGCTTC / A / 55
be85 / TTTCCTCTTATTATTCTTTCATACCC / CTGATTCGAGATTGGGATTGAT / A / 55
be90 / TTGTAATGGAGGAGGTTTCACC / AGAAAATGGTTACGGTCGAAGA / A / 55
al372288 / AAATATCCATTCGATACAATGACC / CAGGCCTCAAGCTAAGAACTGT / A / 55
aa660573 / TTCCGCCCATAGTCTTTGAC / TAAATGTGTCCTGCGTCTGG / A / 55
afct32 / TTTTTGTCCCACCTCATTAG / TTGGTTAGATTCAAAGGGTTAC / A / 55
afct45 / TAAAAAACGGAAAGAGTTGGTTAG / GCCATCTTTTCTTTTGCTTC / A / 55
afctt1 / CCCATCATCAACATTTTCA / TTGTGGATTGGAACGAGT / A / 55
aj388952 / TCAATGGCGAACACTTTCAC / GGAAGAGGGAGAAGGAGATGA / A / 55
al367160 / CCCCATTGACGCATTCTTAC / TCCTCAACCAACCACTTCCT / A / 55
al369471 / AACCAGTGAGTGGATGTGGTC / GTGAAAACCCTTAGCACCGA / A / 55
al371804 / TCATGTTGCAGTTGGAAGGA / TGGTTCTTAATTTTATCCATCATCA / A / 55
al375136 / CATGTTTTTGTTTTGTTGGGG / TTCAGCTTAGCAACAAGTCTCAA / A / 55
al381574 / TGCATTGAAGCAAATTAACGA / TCCACTTACTGGCACTGAAGG / A / 55
aw267840 / GGGATTCGACGCTTATTTCA / GAAGACGGTGTTGGTAGGGA / A / 55
aw586158 / GATCAATTCGTGCAGAAGCA / ATTCATCCTTGCTCGTTTCG / A / 55
aw685684 / ATCGTCCCCACTGTGTCTTC / GTGGGGTTGGTGAGAGTGTT / A / 55
aw685868 / AAGCAAGTTCTGTTGATGGAGA / TTGTGAAAGCCAAAACACCA / A / 55
aw686836 / TTTTATTTGTGGTCATTAGCCTCT / GAAACCAAGATCCCCACACA / A / 55
aw690665 / GGTTTTGGAGACATGACGGT / GTGAAGACTTTGCGGTGGAT / A / 55
aw693450 / TTTTTCACAGCACTGAAGAGG / CCATGGCGTCTACCCATTAT / A / 55
aw693871 / GCATTGAGCTCATTCCATTTC / GGCTGTGGTTCATCTGCTTT / A / 55
aw774443 / ATTCGCAGTGAGCTGATCCT / GACATTTGCAGACCACCATT / A / 55
be202675 / CCTTAAACCCAACTCTCACTCC / AGAACGACGAAGTGGAGGAA / A / 55
be323955 / CACACTCTCTCTTCTCCGGTTC / TGTCGTCAGTGGTGGTTGTTA / A / 55
be942279 / GGTGTGTGCGGTTTAGGACT / CCTCCACATAGCTGGTCGAT / A / 55
bf635198 / ACCCCTGCTGAAACAGCATA / CTCTCCCCTAGCCTCAAAGC / A / 55
bf641851 / GAAGACACAATGAGTAGCAGAGC / TGGCCCATGTTTCTCAGACT / A / 55
bf644149 / CTTTTGCGAAATTTCCTTGC / CCTTGGTGCTTCTCATGTCA / A / 55
bf650422 / ACAACAACGATGGACAACGA / CAGGCATTGGTGGAAACAGT / A / 55
bg447652 / TCTCCGACTTGAAGCCAGTT / ATCCACCTAACCAAAACCCC / A / 55
bg448975 / TCGGATCTGACACGATTTTG / TTGGTTAAAAGATGAAGATGAACG / A / 55
bg454767 / ACTCCCTTAAATCCGTGGCT / CACTGGAACCACGAACCTTT / A / 55
bg584955 / GCTTTCCCTTCCACTCTACTCA / AAGGAGGTGCTGATTTGACG / A / 55
bg645450 / CGTAAACAAAGAAAAGCTTGAGAG / GGCAGGAACAGATCCTTGAA / A / 55
bg647796 / GCAAGAAAGCATAGGCTGAGA / GTGAAGCTGCACGAATTTCA / A / 55
bg648700 / GCTTTTCACACCTCCACTCC / ACGGGAAAGACTCCCACTCT / A / 55
mtic12 / TTCCTCTTTTGACTCATCACCA / CAACAACATGTTCATGCTTAGAAAC / A / 55
mtic135 / GCTGACTGGACGGATCTGAG / CCAAAGCATAAGCATTCATTCA / A / 55
mtic14 / CAAACAAACAACACAAACATGG / CCCATTGATTGGTCAAGGTT / A / 55
mtic84 / TCTGAGAGAGAGACAAACAAAACAA / GGGAAAAGGTGTAGCCATTG / A / 55
aa05 / CCTTCTGCCATTCATTTCACTT / CTTCAAAGGGTCATCAAATCAC / B / 60
al108 / TAAATTGGAGGAACGGGATG / GACAGATCTTGCAACCAGCA / B / 60
aw01 / ACCTGTTCTAAGGGAGATTTCG / CAGGGGAAGCATACAAAACC / B / 60
aw212 / GTCGAAATGGTTGCTTCTCTTT / GGTTAGGGTTTTGGGTTTGAA / B / 60
aw213 / ACCCTTGTGGGTTCTTCTTCTT / CATGTACGGGGATTGTTGTTTT / B / 60
aw235 / CAGTTACGGTGTCATTCTCGTC / TTGGGAGGAGTGTATGATGTTG / B / 60
aw255 / TCTCTCCATCATCACCATCATC / TGCTTGAACTTTGAGTCTTGGA / B / 60
aw310 / CCACTCAACCTCATCTCTCTACC / CAATGCAAGAAACCCTAAAAGC / B / 60
aw325 / TCTGTAAGAGGGTCACTGCGTA / GCTTGTTGTTGTTGTTGATGCT / B / 60
aw352 / ACGTTCCTCCTTCATCTCGTAA / ATCTCCTCGTGTATTCCTTCCA / B / 60
aw776153 / TGGGTGGAGGAAATTACGAC / CCACATATGTTGCTGTTTCCA / B / 60
be118 / CTCCTTTGTAACGCAACAGCAG / TGCAAACTTCACCGAATAGATG / B / 60
bf220 / ATCATCGTCGTCGTGTTTATTG / TGAGTTTTCAGATTCAGCAGGA / B / 60
bf28 / TTTCAATCTTCTCCTTTGATTGC / GGCAGCCATGATACAAGTGA / B / 60
bf56 / TCTCACACCCCAAAAACACA / TCAAAGTTGTTGTTCTGCTTGAA / B / 60
bf69 / CTCTCACCAAACCCACTTCC / TTGAAGTTGGTGGAACAGCA / B / 60
bg105 / CCAATCTCCCCTTTTTCTCC / CATTGCTGTTGGAATTGCTG / B / 60
bg137 / ACTCTTCCTCGCCACTTCAAC / CAGAGCAATAAGAACACCAGGA / B / 60
bg208 / ACACCTCGAACAAGATTCATCC / AGTAACCGCGAACCAAAGAGTA / B / 60
bg234 / GCTGGAATACACCAAGCATGA / GCAACATACCATCCCCTAAAAG / B / 60
bg249 / GGATACAAAATCCACAAGCACA / ACATAAGCGACTGGAACAAACC / B / 60
bg28 / GAGCAAAGGGGTTTGTCTCA / GCAACTCCAGCTGCATCTTT / B / 60
bi111 / GCCTTTAGTGGGATGAGTTCTG / TTTTGCTGAGGTGATGATATGG / B / 60
bi116 / CACACTTTCTCGTTTGCTCTCT / TCAACCCTTCAGATTTTCTTCC / B / 60
bi54 / ACCACCACCACCTTACTCTCC / TTGTCAAACTCCACAACAGACC / B / 60
bi89 / TGGTTACTATTCCCACCATCATC / GTTTTGTCGTTGTGGAGTTTCA / B / 60
mt1b06(2) / GTTTCCACGTGAAAGCCAGT / CATGGGCTGATACAACACACA / B / 60
mt1d06 / CCATGGCTCTTTCCTACCAA / GAAGGTTTTGGGTGGTGATG / B / 60
mt1f11 / TTACCGCTTTTGGATTCTGG / TTGGTGAGAGCTGGTGATTG / B / 60
mt1g05(2) / ATCATTACCGCAGCAAATCC / TTTTTGGAGTTTTGTTGGTGG / B / 60
122161_4 / CCACGTTGTTGAACAGTGGAAATG / GCGAACTTGTTTCCGATGATGC / B / 64
1b08.aga.7-1 / TGGAGGGAAATGATTTAGCG / AACGAAAACGAAAACGAACG / B / 58
1b12.ttc.5-1 / GTCGTCGTAGAGTGGGGTGT / GAGTGGCCATGGATTCAAAC / B / 64
1d11.aata.4-1 / ATTCCCCCACCAACTAATCC / AACTTTTTACATTGACGGTGCAT / B / 60
1f02.tat.6-1 / TGATGCTGTCCTATGCCAAG / TGGAAAAGGCTTTGACTGTTG / B / 60
1f11.gat.5-1 / TGTTCTTCCTCATTTTGGGG / CATCATCGGAATCAGAAGCA / B / 58
1g03.gaa.5-1 / TTTATCGGCGAAGAAGATCG / TCCCGCTTCACTTCACTTTC / B / 58
1g05.tct.12-1 / TCAGAAATTCCCTCCCATTG / AAGAATGACGAAGAGGCGAA / B / 58
1h03.aatt.4-1 / TGATTCAAGGATGGGAAAGC / TGTCTTCCGTGGTCTCACTG / B / 58
2a09.gaa.5-2 / GAGGAGATGATGCAAGTGCC / GCGGTGAAAGAGTGTCCAAT / B / 62
3d03.atc.5-1 / TGTGAACATCAGGAGGTGGA / GTGAATGGTGGTCGTCTTCA / B / 60
3e10.cag.6-1 / AGCATTTGCAGTGCTAGGGT / ACAGCAACAGCAACAACAGC / B / 60
ac149805 / GGCCTTAAAACCTGGGAAAG / GTACTGGATGTGGCTTCCGT / B / 60
ai01 / TTGAAAATTGGGAACGGAAA / GTTGGAGTGGGAAATTGCAG / B / 56
al111 / TGCAGCCAGGTGAATAACAA / CATCTGATGGTGGTGATTGG / B / 58
al84 / CTGCACCCCCTAAAAATCAA / CTCATTGCCCTTCTCACACA / B / 59
aw201 / CCGTCTTTACATGAATCCACAA / CACAGTCATCATCCTTGCTCTC / B / 60
aw326 / GCATATCCATTCCAAGTTCATC / ACTTTCTTCCTCATTGCTCTGC / B / 60
aw347 / GAACGGGTTTGCGATCTTT / CCATGTCTCTCAATCTTCGTCA / B / 58
aw361 / GAGGAGGAAGACGATGATGTTT / CGCTTGGGAAGGAGTAAGAG / B / 60
aw389 / GCAGCCTTCAAATCTCCATAAC / TCACTCTCTCACCAATCACCAC / B / 60
be126 / AGGAGAACGACGAGTCAACTTC / CCTTAAACCCAACTCTCACTCC / B / 64
bf111 / TCAGTGAGAAGGTCGTTCATGT / TGAGAGAGAGTTCGTGGGTTG / B / 62
bf120 / ATTTCAGAGGCAGATGGTGAAT / TAGCAAAATGGGTCAACAAGTG / B / 60
bf132 / AATCCAGCTTTGGAAGACTCAA / TTCTTGTGGTGGTGATGAAAAC / B / 60
bf136 / TGCCAACCAAGATCACACTACT / GAAGGGTTTTGGGAAGAACTTT / B / 60
bf156 / CGTCATCGTATTTGGAACTGAA / TGGTCCTCATTCTTCAACAGAG / B / 60
bf163 / GGCACCTTTGGAACTCATTTT / GCCTTACCCTCTCAACCAATAA / B / 59
bf171 / CACTAGCTTCCACCCTTTGAAT / AGTACCAACAACACCATCATCG / B / 60
bf173 / ACGGTATCTGGAACGATCTCAC / AACTCCGCTCTATGCAGCTATC / B / 64
bf182 / TAGAACGGAATCGAGTGGAGAT / GAGGTTGAGTACGAAGGTGGAG / B / 60
bf200 / AAGAGCAGAAGAAGGTTTGTCG / TACCTAAGCAAGCAAGGCAAAG / B / 60
bf207 / GAGTAGGTTTGGGTTTGGGATT / GTAAATTCAAGGGCCAAGGTC / B / 60
bf208 / GGAGGAAAATCAAAAGGTAGGG / GTCTCATCGTTCAATATACACACAC / B / 60
bf218 / TCGGATTTGGTTTTGAGTTTTC / CTCAGGAGGTGCTGTTCTTCTT / B / 58
bf228 / ATTAGAAGCTCCGTTACCGTCA / ATAACCAACTCCAAACCACACC / B / 60
bf24 / TTGAAAATTGGGAACGGAAA / GTTGGAGTGGGAAATTGCAG / B / 56
bf246 / ATTCCCAATCCCAGCAATAAA / TCTTTGTTCTCTCTTTAGAAGTTATGG / B / 56
bf249 / CGACCTATGACAAATCGAACAC / TCAATCCTTTGAATCCACCAC / B / 60
bf257 / ATGCCAGGATGGTGATACATCT / GGATTTGGGCGTGAGACTATAC / B / 61
bf97 / CTACCTCCAGCAGAACCATGTC / GTAACCATCCTTTGAGTTCGTCTG / B / 60
bg115 / TGCATTTGTTAACGAGTGTGAA / CCACAGAAGAAAGAAGAACTTGC / B / 58
bg132 / TTTTCTTTGACTAGCCCACCAC / TCCTCCTCTGCTTCTTCATTTT / B / 60
bg134 / TTTTCAAGGAGGAGAAGATCCA / ACCCCACCTAACCCTCTACAGT / B / 60
bg142 / TGTGGTGAAGAAACGGATAGAA / AGTATCAATCTTTGGCGCTACC / B / 60
bg143 / GGTAATCGTTGGCGTTGTTTAT / TCAGGTAGTTGACGACGAAGAA / B / 60
bg180 / AGAAGGTGGAACACGTCTCTTC / CTACAAGCCCAGATTTCAAAGG / B / 64
bg183 / TATTGGGACAAAGGGGATAGG / CTCAATGATTGGGAAACCAAGT / B / 61
bg210 / GGTGATTAAGGAGGTGCTGATT / GCTTTCCCTTCCACTCTACTCA / B / 60
bg229 / GAACGGGTTTGCGATCTTT / CCATGTCTCTCAATCTTCGTCA / B / 58
bg232 / TGCCTTTGATTAGTGCTGACAT / CTCTGCTCCCATCTACTTCACA / B / 60
bg242 / CGACCTATGACAAATCGAACAC / TTTGAATCCACCACCAACAAC / B / 60
bg245 / GACTTTTCCAATTCACCAAAGG / GGATCTGGGTTTTGTTTTCTCA / B / 60
bg257 / ATTTCAGAGGCAGATGGTGAAT / TAGCAAAATGGGTCAACAAGTG / B / 60
bg288 / TCGGATGATGATGAAGAAGTGT / GAAGGAGAAAAGGTGAGGGTTT / B / 60
bi103 / GAAGCGATCTAGCAACAGATGA / CCAAACTTCACTCCGAACCTAA / B / 60
bi107 / AGCAGTGATGTCTTGGCTATGT / GTTTCCGGTTCTTTGTCGTTC / B / 60
bi112 / TCCCTTCTTTCTGGTTCCATT / AAATAGCTTTCCAGCGTTTCAC / B / 60
bi113 / AACATCGTAATGAGGAGGAGGA / ACAGTATCAGCAACACCAGCAG / B / 62
bi122 / CAATTTCCTTAGTGGCCGTTAC / TTATTAGCTGGGCTTTTCTTCG / B / 62
bi124 / CTCCAATCATAAACCTCCCAAG / CAGACTCAGAAGATGAAAACAACC / B / 62
bi40 / GTGTCGATCAAGGAGGCAAT / CCAACAAAAATCCCATCACC / B / 60
bi75 / CGTAGGAAGAAGGATCGAGTTC / CCCAATTCAAAACGAAGAACC / B / 64
bi76 / GGGAACGAAAGTTGGTCTTAAA / TCCCATCACATCTTCTTCTCAC / B / 60
cg9500651_1 / TGACAGTCAGGAATTGTCATGC / ATGACAGCTTTAGTGTGGCTAGG / B / 62
tc105099 / AGATAGGAATTTGGGTCGGG / ACAACCATGATGTGGGAATG / B / 60
tc106861 / GCAGGGCTGAGACTCCAGTA / AGCCCTGCTTTTTCTCCTCT / B / 64
tc88874_2 / CTTGCGCGGCCTTCATAATCG / TGCAGAATTTCGATATCGGTGGTCC / B / 64
bg456767 / TCCCTTAAATCCGTGGCTCT / TTCCCATGCAGAAGAAATCC / A / 55
be323955 / CACACTCTCTCTTCTCCGGTTC / TGTCGTCAGTGGTGGTTGTTA / A / 55
al367466 / CCTCCACACTCTCTCTCTTGC / CCGAACAATTCTCCGATGAT / A / 55
aw685868 / AAGCAAGTTCTGTTGATGGAGA / TTGTGAAAGCCAAAACACCA / A / 55
bf19 / ATGCTACCAAGGCTGCTGAT / CACATACTTGAAGGGATGACAAG / A / 55
bg581665 / AACGGTGGTGTGTTTATTGCT / TTCCCATATGCAACAGACCTT / A / 55
a PCR program A is as follow: 4 min at 94°C, followed by 35 cycles with 30 sec at 94°C, 1 min at 55°C and 1 min at 72°C, plus a final elongation step of 7 min at 72°C; PCR program B is as follow: 95ºC for 2 min, followed by 30 cycles with 30 sec at 95 ºC, 45 sec at 60 ºC, and 45 sec at 72 ºC, plus 10 cycles with 30 sec at 95 ºC, 45 sec at 53 ºC, 45 sec at 72 ºC, and finalized with an elongation step of 7 min at 72 ºC