Supplementary Table 1: Location of amino acid residues with greater than 50% conservation amongst 94 BA GPCRs

TM I 1 / TM II / TM III / 2nd iloop / TM V / TM VI / TM VII
L1:39 / 60 / N2:40 / 89 / C3:25 / 100 / P3:57 / 85 / Y5:30 / 66 / T6:36 / 75 / W7:40 / 95
T1:46 / 60 / F2:55 / 55 / W3:28 / 72 / Y3:60 / 92 / S5:42 / 67 / L6:37 / 77 / L7:41 / 79
G1:49 / 90 / S2:45 / 90 / D3:32 / 98 / T3:65 / 60 / S5:43 / 51 / I6:39 / 57 / G7:42 / 85
N1:50 / 100 / L2:46 / 96 / V3:33 / 40 / R3:69 / 54 / S5:46 / 62 / I6:40 / 61 / Y7:43 / 89
L1:52 / 78 / A2:47 / 97 / C3:36 / 53 / F5:47 / 95 / G6:42 / 66 / N7:45 / 92
V1:56 / 98 / A2:49 / 67 / T3:37 / 89 / TM IV / Y5:48 / 61 / F6:44 / 100 / S7:46 / 100
A1:56 / 60 / D2:50 / 100 / A3:38 / 83 / I4:46 / 74 / P5:50 / 94 / C6:47 / 94 / N7:49 / 100
V1:57 / 57 / L2:51/52 / 78 / S3:39 / 100 / V4:49 / 57 / Y5:58 / 93 / W6:48 / 98 / P7:50 / 98
V2:53 / 70 / I3:40 / 98 / W4:50 / 100 / P6:50 / 98 / I7:52 / 76
M2:58 / 77 / L3:41 / 52 / S4:53 / 76 / 3rd iloop / F6:51 / 95 / Y7:53 / 100
1st iloop 2 / P2:59 / 91 / N3:42 / 56 / I4:56 / 53 / I5:61 / 75 / F6:52 / 95 / F7:56 / 60
Φ31:60 / 73 / L3:43 / 87 / S4:57 / 62 / Y5:62 / 77
R1:61 / 62 / 1st eloop 2 / I3:46 / 96 / P4:59/60 / 86 / A5:65 / 62 / 3rd eloop / C-term
L1:63 / 91 / G2:68 / 61 / S3:47 / 71 / E6:30 / 92 / F6:60 / 57 / N7:57 / 74
W2:70 / 100 / D3:49 / 98 / 2nd eloop / K6:61 / 71 / C6:61 / 71 / F7:60 / 91
G2:73 / 81 / R3:50 / 100 / C5:30 / 100 / A6:32 / 81 / C7:26 / 66 / R7:61 / 77
Y3:51 / 94 / K6:34 / 53 / A7:63 / 77
A3:53 / 63 / F7:64 / 82
I3:54 / 59 / L7:68 / 72

1 The conservation of amino acid residues amongst GPCRs is summarized for each transmembrane domain (TM) and loop. Residues exhibiting 100% conservation are in bold lettering. Percentage conservation was calculated from multiple sequence alignments and rounded to the nearest whole number. (CeC24A8.1, AsTA/OA and Bm-ser-2 that lacked complete sequence were omitted from the calculation).

2 iloop -intracellular loop, eloop - extracellular loop.

3Φ aromatic residue