Supplementary Table 2: List of the 274 Tos17 preferred target genes exhibiting more

than 5 Tos17 inserts in both OTL and NIAS insertion line libraries. OTL and NIAScolumns precise the number of occurrence in each collection. Genes are rankedaccording totheir occurrence in the OTL library.

gene / function / OTL / NIAS
Os05g05860 / DNA-directed RNA polymerase II largest subunit, putative / 121 / 106
Os08g41490 / hypothetical protein / 84 / 79
Os02g58170 / hAT family dimerisation domain containing protein, expressed / 82 / 56
Os04g53120 / NB-ARC domain containing protein, expressed / 77 / 76
Os12g01449 / expressed protein / 71 / 64
Os02g57120 / HEAT repeat family protein, expressed / 65 / 50
Os02g02680 / Leucine Rich Repeat family protein, expressed / 61 / 61
Os10g36210 / Valyl-tRNA synthetase, putative / 59 / 31
Os08g41640 / Ubiquitin carboxyl-terminal hydrolase family protein / 54 / 37
Os04g51090 / expressed protein / 54 / 31
Os08g09810 / hAT family dimerisation domain containing protein / 53 / 36
Os08g44020 / LG27/30-like gene, putative, expressed / 53 / 25
Os09g38710 / HEAT repeat family protein, expressed / 51 / 31
Os09g38700 / strubbelig receptor family 5, putative, expressed / 49 / 28
Os08g43000 / NB-ARC domain containing protein, expressed / 46 / 45
Os12g41170 / Root hair defective 3 GTP-binding protein containing protein, expressed / 45 / 62
Os07g45560 / expressed protein / 38 / 39
Os08g09800 / hAT family dimerisation domain containing protein / 38 / 36
Os06g08380 / Beta-1,3-glucan synthase, putative, expressed / 37 / 64
Os01g05980 / Leucine Rich Repeat family protein, expressed / 36 / 18
Os09g21420 / hAT family dimerisation domain containing protein / 32 / 25
Os01g42060 / expressed protein / 32 / 11
Os03g18710 / expressed protein / 31 / 32
Os05g48390 / Ubiquitin-conjugating enzyme family protein, expressed / 29 / 30
Os01g57310 / Rp1, putative / 28 / 28
Os06g02520 / expressed protein / 28 / 25
Os04g50870 / Elongation factor Tu C-terminal domain containing protein, expressed / 27 / 25
Os03g08560 / ATPase 10, plasma membrane-type, putative / 27 / 19
Os01g72680 / NB-ARC domain containing protein / 27 / 12
Os12g06230 / hypothetical protein / 26 / 42
Os02g02910 / expressed protein / 26 / 19
Os06g06590 / expressed protein / 26 / 16
Os06g06710 / hypothetical protein / 26 / 14
Os06g47800 / NB-ARC domain containing protein, expressed / 26 / 12
Os01g51780 / Auxin efflux carrier component 3, putative, expressed / 26 / 11
Os03g03610 / 1,3-beta-glucan synthase component family protein, expressed / 25 / 42
Os06g11520 / LMBR1 integral membrane family protein, putative, expressed / 25 / 25
Os07g03000 / Leucine Rich Repeat family protein, expressed / 24 / 25
Os01g66700 / Glycosyl hydrolase family 20, catalytic domain containing protein, expressed / 24 / 18
Os04g35970 / F-box domain containing protein / 24 / 7
Os02g05040 / cyclin, putative, expressed / 23 / 30
Os07g10550 / cyclin, putative, expressed / 23 / 21
Os04g49430 / Regulator of chromosome condensation family protein, expressed / 23 / 17
Os11g04800 / hypothetical protein / 23 / 9
Os01g10900 / Leucine Rich Repeat family protein, expressed / 23 / 7
Os04g51070 / Helix-loop-helix DNA-binding domain containing protein, expressed / 22 / 23
Os03g01580 / expressed protein / 22 / 16
Os08g05810 / DEAD/DEAH box helicase, putative / 22 / 15
Os01g66680 / S-domain receptor-like protein kinase, putative, expressed / 22 / 9
Os04g52440 / aminotransferase, class III family protein, expressed / 22 / 9
Os09g33830 / anthocyanin-related membrane protein 1, putative, expressed / 22 / 6
Os06g07770 / expressed protein / 21 / 24
Os11g44340 / hypothetical protein / 21 / 18
Os12g43940 / TPR Domain containing protein, expressed / 21 / 17
Os04g35840 / T-complex protein 11 containing protein, expressed / 21 / 15
Os04g53720 / SNF2 domain-containing protein, putative, expressed / 21 / 12
Os03g58600 / Argonaute-like protein At2g27880, putative, expressed / 20 / 50
Os09g37480 / auxin induced protein, putative, expressed / 20 / 45
Os12g43840 / TPR Domain containing protein, expressed / 20 / 36
Os01g42350 / PDR5-like ABC transporter, putative / 20 / 22
Os01g68870 / Leucine Rich Repeat family protein, expressed / 20 / 17
Os07g03010 / hypothetical protein / 20 / 13
Os08g44040 / LG27/30-like gene, putative, expressed / 20 / 8
Os09g37490 / Auxin responsive protein / 19 / 109
Os03g63150 / powdery mildew resistance protein PM3b, putative, expressed / 19 / 46
Os08g41540 / Ubiquitin carboxyl-terminal hydrolase family protein / 19 / 24
Os03g01590 / PAIR1 protein, putative, expressed / 19 / 11
Os12g40070 / B3 DNA binding domain containing protein / 19 / 10
Os03g14700 / calcium-dependent lipid-binding protein, putative / 19 / 7
Os07g09890 / Hexokinase 1, putative, expressed / 19 / 6
Os10g37660 / Trehalase family protein, expressed / 19 / 5
Os06g51310 / PINHEAD protein, putative, expressed / 18 / 24
Os12g10820 / expressed protein / 18 / 18
Os07g26430 / NB-ARC domain containing protein / 18 / 10
Os05g01610 / Chromosome condensation factor, putative / 18 / 8
Os01g57340 / Rp1, putative / 17 / 46
Os01g65850 / Type III restriction enzyme, res subunit family protein / 17 / 25
Os07g33370 / IQ calmodulin-binding motif family protein, expressed / 17 / 15
Os02g43460 / Helicase conserved C-terminal domain containing protein / 17 / 9
Os01g66510 / MLO-like protein 1, putative, expressed / 17 / 5
Os12g40080 / B3 DNA binding domain containing protein / 16 / 31
Os06g48940 / Arabinose kinase, putative, expressed / 16 / 23
Os09g10650 / Phosphatidylinositol-4-phosphate 5-Kinase family protein / 16 / 22
Os03g48310 / Plasma membrane ATPase 3, putative, expressed / 16 / 20
Os03g10570 / meiotic asynaptic mutant 1, putative / 16 / 18
Os04g52450 / aminotransferase, class III family protein, expressed / 16 / 18
Os01g05480 / ternary complex factor MIP1, putative, expressed / 16 / 16
Os02g18000 / NB-ARC domain containing protein, expressed / 16 / 12
Os06g06670 / expressed protein / 16 / 11
Os02g53720 / Protein kinase domain containing protein, expressed / 16 / 7
Os03g06460 / Type I inositol-1,4,5-trisphosphate 5-phosphatase CVP2, putative, expressed / 16 / 7
Os07g49210 / Helicase conserved C-terminal domain containing protein, expressed / 15 / 46
Os06g09450 / Sucrose synthase 1, putative, expressed / 15 / 33
Os04g49320 / tRNA-splicing endonuclease positive effector, putative, expressed / 15 / 13
Os01g73580 / Beta-fructofuranosidase, insoluble isoenzyme 4 precursor, putative, expressed / 15 / 12
Os04g53210 / expressed protein / 15 / 11
Os05g46740 / regulator of chromosome condensation, putative, expressed / 15 / 11
Os06g01966 / Integral membrane protein DUF6 containing protein, expressed / 15 / 10
Os01g02860 / expressed protein / 15 / 10
Os08g43850 / hypothetical protein / 15 / 7
Os08g44160 / hypothetical protein / 15 / 6
Os06g49120 / NmrA-like family protein, expressed / 15 / 5
Os07g39930 / expressed protein / 15 / 5
Os03g53660 / myosin family protein, putative, expressed / 14 / 26
Os02g10630 / C2 domain containing protein, expressed / 14 / 25
Os06g48800 / Cation transport protein, expressed / 14 / 19
Os07g02990 / expressed protein / 14 / 14
Os02g05686 / expressed protein / 14 / 11
Os09g26000 / Receptor family ligand binding region containing protein / 14 / 9
Os01g25720 / NB-ARC domain containing protein, expressed / 14 / 9
Os07g49220 / Eukaryotic translation initiation factor 2 alpha subunit, eIF2, putative / 14 / 8
Os10g40324 / hypothetical protein / 13 / 18
Os02g38710 / Protein phosphatase 2C containing protein, expressed / 13 / 17
Os06g01840 / Integral membrane protein DUF6 containing protein, expressed / 13 / 14
Os02g18070 / NB-ARC domain containing protein, expressed / 13 / 14
Os07g08000 / Protein kinase domain containing protein, expressed / 13 / 11
Os03g53220 / U5 small nuclear ribonucleoprotein 200 kDa helicase, putative / 13 / 10
Os07g46130 / expressed protein / 13 / 10
Os01g50980 / Cullin family protein / 13 / 9
Os12g42660 / incomplete root hair elongation, putative, expressed / 13 / 9
Os07g45390 / hypothetical protein / 13 / 6
Os08g44150 / Endomembrane protein 70 containing protein, expressed / 13 / 5
Os03g15810 / ATPase, AAA family protein, expressed / 13 / 5
Os03g14790 / Piwi domain containing protein, expressed / 12 / 16
Os08g10260 / NB-ARC domain containing protein, expressed / 12 / 16
Os10g35200 / Katanin p80 WD40-containing subunit B1 homolog 1, putative, expressed / 12 / 15
Os02g01270 / START domain-containing protein, putative, expressed / 12 / 14
Os11g05480 / Transcription factor HBP-1b, putative, expressed / 12 / 13
Os02g04840 / Arabinose kinase, putative, expressed / 12 / 13
Os04g37960 / expressed protein / 12 / 12
Os04g53760 / Kinesin-1, putative, expressed / 12 / 11
Os04g54200 / diacylglycerol kinase, putative, expressed / 12 / 11
Os05g04610 / ABC transporter family protein, expressed / 12 / 7
Os02g04630 / Sodium/calcium exchanger protein, expressed / 12 / 6
Os06g41640 / NB-ARC domain containing protein, expressed / 11 / 37
Os04g52230 / Terpene synthase family, metal binding domain containing protein, expressed / 11 / 13
Os01g25740 / powdery mildew resistance protein PM3b, putative / 11 / 13
Os01g10710 / D-mannose binding lectin family protein / 11 / 9
Os10g39560 / Serine carboxypeptidase family protein / 11 / 8
Os02g06120 / Leucine Rich Repeat family protein / 11 / 8
Os02g02250 / expressed protein / 11 / 7
Os04g53560 / expressed protein / 11 / 5
Os08g01270 / Protein kinase domain containing protein, expressed / 10 / 19
Os07g35610 / Kelch motif family protein, expressed / 10 / 14
Os01g05780 / expressed protein / 10 / 13
Os01g67120 / Rhodanese-like domain containing protein, expressed / 10 / 11
Os01g59580 / Protein kinase domain containing protein / 10 / 10
Os02g39480 / Protein phosphatase 2C containing protein / 10 / 9
Os04g35430 / PHD-finger family protein, expressed / 10 / 9
Os02g55050 / F-box domain containing protein, expressed / 10 / 8
Os05g40240 / hypothetical protein / 10 / 6
Os04g41250 / U-box domain containing protein, expressed / 10 / 5
Os02g56570 / RabGAP/TBC domain-containing protein, putative, expressed / 10 / 5
Os01g05870 / Leucine Rich Repeat family protein, expressed / 9 / 17
Os02g02780 / protein kinase family protein, putative, expressed / 9 / 16
Os07g39950 / Mitotic checkpoint protein BUB3, putative, expressed / 9 / 16
Os11g03820 / D-mannose binding lectin family protein, expressed / 9 / 16
Os06g48920 / hypothetical protein / 9 / 15
Os03g61840 / hypothetical protein / 9 / 11
Os05g31610 / Leucine Rich Repeat family protein, expressed / 9 / 11
Os06g07200 / Syntaxin 132, putative, expressed / 9 / 10
Os04g59000 / protein kinase family protein, putative, expressed / 9 / 10
Os08g07480 / DNA-directed RNA polymerase, beta subunit family protein, expressed / 9 / 8
Os11g03610 / F-box domain containing protein, expressed / 9 / 8
Os12g39980 / Kinesin motor domain containing protein, expressed / 9 / 7
Os01g71270 / Eukaryotic peptide chain release factor subunit 1-2, putative, expressed / 9 / 6
Os02g40410 / expressed protein / 9 / 6
Os02g06340 / EH-domain containing protein 1, putative, expressed / 9 / 6
Os01g02300 / receptor kinase ORK10, putative, expressed / 9 / 5
Os08g09820 / hypothetical protein / 9 / 5
Os04g35210 / Leucine Rich Repeat family protein, expressed / 9 / 5
Os02g12350 / histone deacetylase, putative, expressed / 9 / 5
Os03g56724 / hypothetical protein / 9 / 5
Os04g38270 / expressed protein / 9 / 5
Os09g27950 / Avr9 elicitor response protein, putative, expressed / 9 / 5
Os04g51340 / Pectinacetylesterase protein, putative, expressed / 9 / 5
Os02g58160 / expressed protein / 8 / 18
Os04g52240 / Terpene synthase family, metal binding domain containing protein / 8 / 16
Os08g05690 / ABC transporter family protein / 8 / 15
Os01g50100 / multidrug resistance protein 1 homolog, putative, expressed / 8 / 12
Os03g24870 / Sugar transporter family protein, expressed / 8 / 10
Os07g47590 / heavy metal-induced putative protein 1, putative, expressed / 8 / 8
Os02g04680 / SBP domain containing protein, expressed / 8 / 8
Os12g37840 / Boron transporter 1, putative, expressed / 8 / 7
Os11g04030 / major facilitator superfamily antiporter, putative, expressed / 8 / 7
Os09g17630 / Receptor-like protein kinase 2, putative, expressed / 8 / 7
Os05g50890 / GH3 auxin-responsive promoter family protein, expressed / 8 / 7
Os02g50910 / chromosome-associated kinesin, putative, expressed / 8 / 6
Os03g17550 / Serine/threonine-protein kinase RLCKVII, putative, expressed / 8 / 6
Os01g05960 / Leucine Rich Repeat family protein, expressed / 8 / 5
Os01g49200 / Microtubule associated protein containing protein, expressed / 8 / 5
Os01g67100 / expressed protein / 8 / 5
Os09g28830 / Serine carboxypeptidase family protein / 8 / 5
Os01g56730 / expressed protein / 7 / 15
Os11g37000 / DnaJ domain containing protein, expressed / 7 / 15
Os04g39900 / Non-cyanogenic beta-glucosidase precursor, putative, expressed / 7 / 13
Os03g08390 / expressed protein / 7 / 11
Os06g49130 / TAZ zinc finger family protein, expressed / 7 / 11
Os01g04900 / peptidase M50 family protein, putative, expressed / 7 / 10
Os04g47180 / expressed protein / 7 / 10
Os06g49420 / NB-ARC domain containing protein, expressed / 7 / 9
Os11g36140 / Leucine Rich Repeat family protein, expressed / 7 / 9
Os07g39940 / Helix-loop-helix DNA-binding domain containing protein / 7 / 8
Os06g40570 / C2 domain-containing protein, putative, expressed / 7 / 8
Os01g10700 / D-mannose binding lectin family protein / 7 / 8
Os08g34370 / SET domain containing protein / 7 / 7
Os09g32910 / F-box domain containing protein, expressed / 7 / 7
Os06g01780 / expressed protein / 7 / 7
Os01g66640 / D-mannose binding lectin family protein, expressed / 7 / 7
Os07g46580 / ATP-dependent RNA helicase DDX47, putative, expressed / 7 / 6
Os06g05359 / NB-ARC domain containing protein, expressed / 7 / 6
Os07g47100 / sodium/hydrogen exchanger 3 family protein, expressed / 7 / 6
Os01g48910 / AMP-binding enzyme family protein, expressed / 7 / 5
Os02g06200 / Leucine Rich Repeat family protein, expressed / 7 / 5
Os02g50560 / DEAD/DEAH box helicase, putative, expressed / 7 / 5
Os12g32680 / NB-ARC domain containing protein / 7 / 5
Os06g48870 / CW-type Zinc Finger family protein, expressed / 7 / 5
Os07g08540 / B3 DNA binding domain containing protein / 7 / 5
Os03g14690 / V-type ATPase 116kDa subunit family protein, expressed / 6 / 26
Os12g38460 / RNA recognition motif family protein / 6 / 24
Os05g46720 / phosphatidylinositol transfer-like protein III, putative, expressed / 6 / 15
Os02g54900 / protein kinase family protein, putative, expressed / 6 / 14
Os05g41580 / Calcium-transporting ATPase 4, plasma membrane-type, putative, expressed / 6 / 12
Os03g01720 / hypothetical protein / 6 / 10
Os01g04920 / glycosyl transferase, group 1 family protein, expressed / 6 / 10
Os04g53160 / NB-ARC domain containing protein, expressed / 6 / 9
Os09g38010 / NAC-domain containing protein 78, putative, expressed / 6 / 9
Os04g52210 / Terpene synthase family, metal binding domain containing protein, expressed / 6 / 9
Os04g36740 / Potassium channel SKOR, putative, expressed / 6 / 8
Os03g18300 / pherophorin, putative, expressed / 6 / 8
Os04g34390 / D-mannose binding lectin family protein, expressed / 6 / 7
Os01g05720 / protein tyrosine phosphatase, putative / 6 / 7
Os10g39470 / expressed protein / 6 / 6
Os02g49040 / Protein kinase domain containing protein / 6 / 6
Os04g55840 / expressed protein / 6 / 6
Os01g06490 / Serine carboxypeptidase II precursor, putative, expressed / 6 / 6
Os12g10740 / Leucine Rich Repeat family protein, expressed / 6 / 6
Os01g05620 / NBS-LRR disease resistance protein, putative, expressed / 6 / 6
Os10g02970 / Leucine Rich Repeat family protein / 6 / 6
Os04g35860 / T-complex protein 11 containing protein, expressed / 6 / 5
Os01g02760 / receptor-like kinase, putative, expressed / 6 / 5
Os10g34220 / expressed protein / 6 / 5
Os05g37170 / Transcription factor HBP-1b, putative, expressed / 6 / 5
Os07g48780 / Calmodulin, putative, expressed / 6 / 5
Os03g61670 / Calreticulin precursor, putative, expressed / 6 / 5
Os11g46230 / calmodulin-binding protein MPCBP, putative, expressed / 6 / 5
Os10g35170 / N-acetyl-gamma-glutamyl-phosphate reductase, chloroplast precursor, putative, expressed / 5 / 25
Os02g50330 / RNA-directed RNA polymerase 2, putative, expressed / 5 / 16
Os08g10510 / aminotransferase, class III family protein, expressed / 5 / 12
Os07g38830 / hydrolase, alpha/beta fold family protein, putative, expressed / 5 / 12
Os03g07350 / glycosyl transferase family 2 protein, putative, expressed / 5 / 9
Os02g10920 / Zinc finger protein ZF2, putative, expressed / 5 / 9
Os04g38640 / Trypsin family protein, expressed / 5 / 8
Os07g06300 / Peroxidase family protein / 5 / 8
Os01g47820 / S-locus-like receptor protein kinase, putative, expressed / 5 / 7
Os10g41060 / expressed protein / 5 / 7
Os05g12770 / NB-ARC domain containing protein / 5 / 7
Os10g25220 / SAP domain containing protein, expressed / 5 / 6
Os01g45990 / Potassium channel AKT6, putative, expressed / 5 / 6
Os01g49280 / Zinc finger, C3HC4 type family protein, expressed / 5 / 6
Os03g40670 / Glycerophosphoryl diester phosphodiesterase family protein, expressed / 5 / 6
Os08g43730 / expressed protein / 5 / 6
Os11g38630 / hypothetical protein / 5 / 6
Os09g30446 / Sodium/hydrogen exchanger family protein / 5 / 6
Os03g63240 / NB-ARC domain containing protein, expressed / 5 / 6
Os05g30390 / Glycosyl hydrolase family 1 protein / 5 / 5
Os10g36280 / Leucine Rich Repeat family protein / 5 / 5
Os02g40770 / Pre-SET motif family protein, expressed / 5 / 5
Os04g51040 / wall-associated kinase 1, putative, expressed / 5 / 5
Os02g51000 / Nuclear movement protein, expressed / 5 / 5
Os03g18190 / Importin-beta N-terminal domain containing protein / 5 / 5
Os09g33530 / esterase/lipase/thioesterase family protein, putative, expressed / 5 / 5
Os06g02510 / 60S ribosomal protein L13-2, putative, expressed / 5 / 5
Os09g19710 / hypersensitive-induced response protein, putative, expressed / 5 / 5