Supplementary table 1: Genomic organization of the complete genome sequence of PPMV-1/pigeon/Bhopal/01/15.
mRNA characteristics / Deduced ProteinGenes / Hexamer phasing / Length
(nt) / 5'UTR
(nt) / ORF
(nt) / 3'UTR
(nt) / Intergenic
(nt) / Size
(amino acid) / Weight
(kDa)
Leader / - / 55 / - / - / - / - / - / -
N / 2 / 1753 / 66 / 1470 / 217 / 1 / 489 / 53.4
P / 4 / 1451 / 83 / 1188 / 180 / 1 / 395 / 42.1
M / 4 / 1241 / 34 / 1095 / 112 / 1 / 364 / 39.5
F / 4 / 1792 / 46 / 1662 / 84 / 31 / 553 / 58.9
HN / 3 / 2002 / 91 / 1716 / 195 / 47 / 571 / 62.7
L / 6 / 6703 / 11 / 6615 / 77 / - / 2204 / 248.8
Trailer / - / 114 / - / - / - / - / - / -
Supplementary table2: Analyses of functional domains within the envelope glycoproteins of PPMV-1/pigeon/Bhopal/01/15. Fusion protein (A),
Haemaglutinin Neuraminidase protein (B). Substitution mutations are highlighted in bold and grey.
(A)Fusion Protein / SequenceFusion peptide / Consensus sequence / 117FIGAVIGSVALGVATAAQITAAAAL141
PPMV Bhopal / 117FIGAIIGGVALGVATSAQITAAAAL141
Heptad Repeat a (HRa) / Consensus Sequence / 143QANQNAANILRLKESIAATNEAVHEVTDGLSQLAVAVGKMQQF185
PPMV Bhopal / 143QANRNAANILRLKESIAATNEAVHEVTDGLSQLAVAIGKMQQF185
Heptad Repeat b (HRb) / Consensus Sequence / 268LITGNPILYDSQTQLLGIQVNLPSVGNLNNMR299
PPMV Bhopal / 268LITGHPILYDSQTQLLGIQVNLPSVGNLNNMR299
Heptad Repeat c (HRc) / Consensus Sequence / 471NNSISNALDKLAESNSKLDKVNVKLTSTSA500
PPMV Bhopal / 471NNSISNALDKLAESNSKLDKVNVRLTSTSA500
Transmembrane Domain Tm / Consensus Sequence / 501LITYIVLTVISLVFGALSLVL521
PPMV Bhopal / 501LITYIVLTVISLVFGALSLVF521
(B)Haemagglutinin Neuraminidase Protein / Sequence
Transmembrane Domain / Consensus sequence / 25FRIAVLLLIVMTLAISAAALV45
PPMV Bhopal / 25FRIAVLLLIVMALAISAAVLV45
Major
Neutralizing Epitopes / Consensus Sequence / 193LSGCRDHSH201
PPMV Bhopal / 193LSGCRDHSH201
Consensus Sequence / 263N, 287D, 321K, 332G, 333R
PPMV Bhopal / 263K, 287D, 321K, 332G,333N
Consensus Sequence / 346DEQDYQIR 353
PPMV Bhopal / 346DEQSYQIR 353
Consensus Sequence / 354K, 494G/D
PPMV Bhopal / 354K, 494D
Consensus Sequence / 513RITRVSSSS 521
PPMV Bhopal / 513RVTRVSSSS 521
Consensus Sequence / 569D
PPMV Bhopal / 569D