Supplementary Table 1. Bacterial strains, plasmids and primers used in this study

Stain or plasmid / Description / Reference or source
Strains
Pseudomonas putida / source for pp0596 gene / Lab collection
Clostridiumacetobutylicum / source for mmsB gene / Lab collection
E. coli DH5α / F-supE44 ΔlacU169 (Φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 / Invitrogen
E. coli JM109 (DE3) / recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-proAB)[F’traD36 proAB+ lacIq lacZΔM15]λ(DE3) / Promega
Q1400 / E. coli JM109 (DE3) bearing pWQ502 / this study
Q1401 / E. coli JM109 (DE3) bearing pWQ503 / this study
Q1402 / E. coli JM109 (DE3) bearing pWQ504 / this study
Q1405 / E. coli JM109 (DE3) bearing pWQ506 / this study
Q1423 / E. coli JM109 (DE3) bearing pWQ507 / this study
Q1760 / E. coli JM109 (DE3) bearing pWQ510 / this study
Q1761 / E. coli JM109 (DE3) bearing pWQ511 / this study
Q1598 / E. coli JM109 (DE3) bearing pWQ512 / this study
Q1749 / E. coli JM109 (DE3) bearing pWQ513 / this study
Q1430 / E. coli JM109 (DE3) bearing pHP302 and pWQ507 / this study
Q1457 / E. coli JM109 (DE3) bearing pWQ507 and pWQ514 / this study
Q1911 / E. coli JM109 (DE3) bearing pHP302 and pWQ513 / this study
Plasmids
pACYCDuet-1 / repp15A CmRlacI PT7 / Novagen
pETDuet / reppBR322 ApRlacI PT7 / Novagen
pTrc His2 B / reppBR322 ApRlacI PTrc / Novagen
pHP301 / reppBR322 ApRlacI PT7 phaC1 / a
pHP302 / reppBR322 ApR lacI PT7phaC1prpE / a
pMCR / source for mcr-N / b
pKS1 / source for pcs’ / c
pWQ501 / reppBR322 ApRlacI PT7 PP0596 / this study
pWQ502 / reppBR322 ApRlacI PT7 ydfG PT7PP0596 / this study
pWQ503 / reppBR322 ApRlacI PT7 mmsB PT7PP0596 / this study
pWQ504 / reppBR322 ApRlacI PT7 mcr-NPT7PP0596 / this study
pWQ505 / reppBR322 ApRlacI PT7 gabT / this study
pWQ506 / reppBR322 ApRlacI PT7 mcr-N PT7gabT / this study
pWQ507 / repp15A CmRlacI PT7 ydfGPT7PP0596 / this study
pWQ508 / repp15A CmRlacI PTrc ydfGPT7PP0596 / this study
pWQ509 / repp15A CmRlacI PRe ydfGPT7PP0596 / this study
pWQ510 / repp15A CmRlacI PTrc ydfG PTrc PP0596 / this study
pWQ511 / repp15A CmRlacI PRe ydfG PRe PP0596 / this study
pWQ512 / repp15A CmRlacI PT7 ydfG panDPT7PP0596 / this study
pWQ513 / repp15A CmRlacI PT7 ydfG panD panMPT7PP0596 / this study
pWQ514 / reppBR322 ApRlacI PT7 phaC1 pcs’ / this study
Primers
pp0596 cloning
pp-F / 5’-CATGGATCCGAACATGCCCGAAACTGGTCCTG-3’
pp-R / 5’-CAGTAAGCTTAGTCGATCAGGTTCAGGGTTTC-3
gabT cloning
gabT-F / 5’-CATCAGATCTCCATCACCATCATCACCACATGGAAGAAATAAATG-3’
gabT-R / 5’-CCCTCGAGTTAAATCGCTATTCTTATAGCTTCT-3
ydfG cloning
ydfG-F / 5’-CATGGATCCGATGATCGTTTTAGTAACTGGA-3’
ydfG-R / 5’-CAGTAAGCTTACTGACGGTGGACATTCAGTC-3’
mmsBcloning
mmsB-F / 5’-CATGGATCCGATGCGTATCGCATTCATCG-3’
mmsB-R / 5’-CAGTAAGCTTACTCCTTCTTGCGATAACC-3’
mcr-Ncloning
mcr-N-F / 5’-GGCAGATCTCAGCGGAACAGGACGAC-3’
mcr-N-R / 5’-CTGAAGCTTATCCGACCGATGCACTGC-3’
panDcloning
panD-F / 5’-CTGAAGCTTTAATACGACTCACTATAAGGGTAGAAAGGTAGA-3’
panD-R / 5’-CTGAAGCTTTCAAGCAACCTGTACCGG-3’
panMcloning
panM-F / 5’-CATGCGGCCGCAATGAAGCTGACCATCATTCG-3’
panM-R / 5’-CATGCGGCCGCTTAACACTTCTCCCAGCCG-3’
pcs’cloning
pcs’-F / 5’-CATGGATCCATATCGAAGGTCGTCATATG-3’
pcs’-R / 5’-CAGTAAGCTTATTCGATGATCTGCTGC-3’
PTrccloning
PTrc-F / 5’-CATCCTGCATTAGGAGCTGTTGACAATTAATC-3’
PTrc-R / 5’-GGCCCATGGTTATTCCTCCTTATTTAAT-3’
PTrc(2)cloning
PTrc(2)-F / 5’-CATCCTTAAGAGCTGTTGACAATTAATC-3’
PTrc(2)-R / 5’-CATCCATATGTTATTCCTCCTTATTTAAT-3’
PRecloning
PRe-F / 5’-CATCCTGCATTAGGTCTCGCCTATGCTCTGGG-3’
PRe-R / 5’-GGCCCATGGGATTTGATTGTCTCTCTGCCG-3’
PRecloning
PRe(2)-F / 5’-CATCCTTAAGTCTCGCCTATGCTCTGGG-3’
PRe(2)-R / 5’-CATCCATATGGATTTGATTGTCTCTCTGCCG-3’

a This plasmid was constructed in our previous study (Wang Q et al. J Microbiol 50:693-7)

b This plasmid was constructed in our previous study (Liu CS et al.PLoS One8:e75554)

c This plasmid was kindly supplied by Dr. Birgit Alber (Schneider KC et al.J Bacteriol194:225-232)

Supplementary Fig. 1 The 1H (a) and 13C (b) NMR spectra of P3HP synthesized by Q1911 in CDCl3 solution. The chemical shift assignment for each proton and carbon resonance was showed