Supplementary materials
<Microbial Ecology>
Analysis of Medium-Chain-Length Polyhydroxyalkanoate Producing Bacteria in Activated Sludge Samples Enriched by Aerobic Periodic Feeding
Sun Hee Lee, Jae Hee Kim, Chung-Wook Chung, Do Young Kim, Young Ha Rhee
Corresponding author
Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Republic of Korea
E-mail address : (Y.H.Rhee)
Table S1. Pure culture of isolated strains using nonanoic acid as carbon source.
strain / PHA composition (mol %)3HB / 3HV / 3HHp / 3HN
M-A / 27.0 / 73.0
M-1 / 10.8 / 21.2 / 20.5 / 47.5
D-11 / 29.6 / 70.4
D-16 / 22.8 / 23.1 / 18.6 / 35.6
A-13 / 28.5 / 71.5
A-17 / 13.6 / 21.7 / 21.4 / 43.3
P-5 / 4.6 / 29.7 / 65.7
P-7 / 12.9 / 10.2 / 20.5 / 56.4
3HB, 3-hydroxybutyrate; 3HV, 3-hydroxyvalerate; 3HHp, 3-hydroxyheptanoate; 3HN, 3-hydroxynonanoate.
The flask culture was grown in a 500 ml baffle flask containing 200ml of medium for 36 h on rotary shaker (200rpm) at 30°C in which 2 g/L nonanoic acid was added as the sole carbon substrate.
Table S2. Phylogenetic affiliation of 16S rRNA gene sequences from DGGE bands marked in Fig. S2
Band / Closet relative / Accession No. / Similarity (%)Methanol
1 / Methylobacillus sp. / EU194898 / 94
2 / Methylobacillus sp. / EU194898 / 95
3 / Methylobacillus sp. / EU194898 / 97
4 / Methylobacillus sp. / EU194898 / 97
5 / Methylobacillus flagellatus / CP000284 / 92
6 / Uncultured Methylobacillus sp. / EF019004 / 97
Acetic acid
1 / Uncultured Bacteroidetes / AM085984 / 91
2 / Thauera sp. / AB287434 / 99
3 / Pseudomonas sp. / EU140960 / 87
4 / Thauera sp. / AB287434 / 95
5 / Acinetobacter sp. / FJ222394 / 93
6 / Uncultured bacterium / AF390917 / 98
Glucose
1 / Lactobacillus salivarius / CP000233 / 92
2 / Uncultured gamma proteobacterium / AF469301 / 84
3 / Lactobacillus camelliae / AB257864 / 93
4 / Lactobacillus manihotivorans / EU195444 / 93
5 / Lactobacillus manihotivorans / EU195444 / 93
6 / Lactobacillus manihotivorans / EU195444 / 98
7 / Alcaligenes sp. / AB166879 / 93
Gluconic acid
1 / Lactobacillus fermentum / AP008937 / 84
2 / Enterococcus italicus / FJ749495 / 91
3 / Lactobacillus fermentum / AP008937 / 84
4 / Lactobacillus fermentum / AP008937 / 84
5 / Alcaligenes sp. / AB166879 / 96
Fig. S1. Analysis of microbial structure of different SBRs fed methanol, acetic acid, glucose or gluconic acid.
Fig. S2. Survival rate of Pseudomonas spp. under low nutrient conditions. ● P. aeruginosa DM1, ○ P. alcaligenes LB19, ▼ P. chlororaphis HS21, △ P. oleovorans ATCC 29347, ■ P. putida KCTC 2407. The flask culture was grown in a 500 ml baffle flask containing 200 ml of medium and 0.1 g/L nonanoic acid at 30°C for 32 days.