Supplementary Material
Table S1. The structure and subcellular location of 72 SODs. The type of metal cofactor and the oligomeric form were identified through alignment with the known X-ray structures of 11 SOD proteins. The target signals were predicted using PSORT II, TargetP and MitoProt II. The predicted subcellular location and calculated mitochondrial probability scores (in parentheses) are shown for each type of analysis (Mito (mitochondrial), Chlp (chloroplastic), Peox (peroxisomal)) (None (no signal), Cypl (cytoplasmic), Secr (secretory), Cysk (cytoskeletal), Enre (endoplasmic reticulum), Exce (extracellular, including cell wall) ND (not determined). Conflicting analyses with high confidence levels are underlined.
Accession No. / SOD Protein / Lineage / Length (aa) / Properties / Subcellular LocationMetal / Oligomer / PSORT II / TargetP / MitoProt II
FJ377710 / NbMnSOD1 (Nosema bombycis MnSOD1) / Microsporidia / 225 / Mn / dimer / Cypl (0.043) / None (0.074) / None (0.0114)
FJ377711 / NbMnSOD2 (Nosema bombycis MnSOD2) / Microsporidia / 225 / Mn / ND / Cypl (0.087) / None (0.103) / None (0.0361)
NP_586414 / EcMnSOD (Encephalitozoon cuniculi MnSOD) / Microsporidia / 233 / Mn / dimer / Cypl (0.087) / None (0.049) / None (0.1098)
AAY27408 / AlMnSOD (Antonospora locustae MnSOD) 1 / Microsporidia / 119 / Mn / ND / ND / ND / ND
AAY57577 / PnMnSOD1 (Phytophthora nicotianae MnSOD1) / Stramenopila / 213 / Mn / dimer / Cypl (0.000) / None (0.073) / None (0.0613)
AAY57576 / PnMnSOD2 (Phytophthora nicotianae MnSOD2) 2 / Stramenopila / 187 / Mn / tetramer / Cypl (0.043) / None (0.077) / ND
XP_001619321 / NvMnSOD1 (Nematostella vectensis MnSOD1) / Metazoa / 202 / Mn / dimer / Cypl (0.087) / None (0.084) / None (0.0195)
XP_001641079 / NvMnSOD2 (Nematostella vectensis MnSOD2) / Metazoa / 221 / Mn / tetramer / Mito (0.739) / Mito (0.835) / Mito (0.9985)
EAR90261 / TtFeSOD (Tetrahymena thermophila FeSOD) 3 / Alveolata / 219 / Fe / tetramer / Mito (0.652) / None (0.414) / Mito (0.9853)
P19666 / TpFeSOD (Tetrahymena pyriformis FeSOD) 2,3 / Alveolata / 196 / Fe / tetramer / Cypl (0.043) / None (0.063) / ND
NP_989542 / Gallus gallus / Metazoa / 224 / Mn / tetramer / Mito (0.652) / Mito (0.925) / Mito (0.8881)
NP_001083968 / Xenopus laevis / Metazoa / 224 / Mn / tetramer / Mito (0.435) / Mito (0.874) / Mito (0.9560)
P04179 / Homo sapiens mitochondria / Metazoa / 222 / Mn / tetramer / Mito (0.391) / Mito (0.762) / Mito (0.9626)
NP_956270 / Danio rerio / Metazoa / 224 / Mn / tetramer / Mito (0.435) / Mito (0.899) / Mito (0.9835)
XP_972440 / Tribolium castaneum / Metazoa / 215 / Mn / tetramer / Mito (0.348) / Mito (0.528) / Mito (0.9495)
XP_393570 / Apis mellifera / Metazoa / 218 / Mn / tetramer / Mito (0.478) / Mito (0.564) / Mito (0.9492)
AAA57131 / Oryza sativa / Plantae / 231 / Mn / tetramer / Exce (0.000) / Mito (0.708) / Mito (0.9085)
NP_187703 / Arabidopsis thaliana / Plantae / 231 / Mn / tetramer / Cypl (0.304) / Mito (0.796) / Mito (0.9609)
NP_011872 / Saccharomyces cerevisiae / Fungi / 233 / Mn / tetramer / Mito (0.435) / Mito (0.695) / Mito (0.9486)
CAG99194 / Kluyveromyces lactis / Fungi / 226 / Mn / tetramer / Mito (0.696) / Mito (0.729) / Mito (0.9869)
CAG90212 / Debaryomyces hansenii / Fungi / 228 / Mn / tetramer / Mito (0.739) / Mito (0.806) / Mito (0.9919)
EAK99504 / Candida albicans / Fungi / 234 / Mn / tetramer / Mito (0.870) / Mito (0.945) / Mito (0.9977)
CAG82903 / Yarrowia lipolytica / Fungi / 229 / Mn / tetramer / Mito (0.739) / Mito (0.781) / Mito (0.9967)
EAL89634 / Aspergillus fumigatus / Fungi / 229 / Mn / tetramer / Mito (0.870) / Mito (0.949) / Mito (0.9967)
AAW83518 / Paracoccidioides brasiliensis / Fungi / 227 / Mn / tetramer / Mito (0.609) / Mito (0.875) / Mito (0.9812)
EAA73238 / Gibberella zeae / Fungi / 230 / Mn / tetramer / Mito (0.913) / Mito (0.944) / Mito (0.9890)
AAO47725 / Cordyceps militaris / Fungi / 230 / Mn / tetramer / Mito (0.826) / Mito (0.916) / Mito (0.9976)
EAA48554 / Magnaporthe grisea / Fungi / 227 / Mn / tetramer / Mito (0.913) / Mito (0.937) / Mito (0.9948)
EAA30249 / Neurospora crassa / Fungi / 228 / Mn / tetramer / Mito (0.870) / Mito (0.900) / Mito (0.9988)
CAB62411 / Schizosaccharomyces pombe / Fungi / 218 / Mn / tetramer / Mito (0.870) / Mito (0.849) / Mito (0.9879)
EAA69573 / Gibberella zeae / Fungi / 254 / Mn / tetramer / Exce (0.000) / Secr (0.046) / None (0.0895)
EAA32343 / Neurospora crassa / Fungi / 245 / Mn / tetramer / Cypl (0.087) / None (0.127) / None (0.7150)
EAA53420 / Magnaporthe grisea / Fungi / 213 / Mn / tetramer / Cypl (0.087) / None (0.053) / None (0.0926)
AAC36585 / Penicillium chrysogenum / Fungi / 210 / Mn / tetramer / Cypl (0.174) / None (0.074) / None (0.0730)
Q92450 / Aspergillus fumigatus / Fungi / 210 / Mn / tetramer / Cypl (0.087) / None (0.095) / None (0.0914)
AAL56985 / Blumeria graminis / Fungi / 216 / Mn / tetramer / Cysk (0.000) / None (0.068) / None (0.0849)
ABK76972 / Cenarchaeum symbiosum / Archaea / 206 / Mn / ND / Cypl (0.043) / None (0.061) / None (0.0940)
ZP_02024558 / Candidatus Nitrosopumilus maritimus / Archaea / 207 / Mn / ND / Cypl (0.130) / None (0.056) / None (0.1568)
Q42684 / Chlamydomonas reinhardtii / Plantae / 218 / Mn / dimer / Cypl (0.130) / None (0.088) / None (0.0141)
ZP_01089421 / Blastopirellula marina / Bacteria / 202 / Mn / dimer / Cypl (0.087) / None (0.101) / None (0.0496)
ZP_01856783 / Planctomyces maris / Bacteria / 203 / Mn / dimer / Cypl (0.000) / None (0.105) / None (0.0672)
YP_544498 / Methylobacillus flagellatus / Bacteria / 204 / Mn / dimer / Cypl (0.130) / None (0.074) / None (0.0484)
ZP_01519759 / Comamonas testosteroni / Bacteria / 211 / Mn / dimer / Cypl (0.043) / None (0.066) / None (0.0444)
ZP_01581157 / Delftia acidovorans / Bacteria / 211 / Mn / dimer / Cypl (0.130) / None (0.057) / None (0.0904)
P00448 / Escherichia coli / Bacteria / 206 / Mn / dimer / Cypl (0.000) / None (0.095) / None (0.1137)
P53655 / Yersinia enterocolitica / Bacteria / 207 / Mn / dimer / Cypl (0.000) / None (0.091) / None (0.0817)
AAQ14590 / Tatumella ptyseos / Bacteria / 205 / Mn / dimer / Cypl (0.043) / None (0.099) / None (0.0944)
NP_907960 / Wolinella succinogenes / Bacteria / 232 / Mn / dimer / Exce (0.000) / Secr (0.053) / None (0.4152)
ZP_01463295 / Stigmatella aurantiaca / Bacteria / 247 / Mn / dimer / Mito (0.348) / Secr (0.175) / None (0.4080)
YP_760332 / Hyphomonas neptunium / Bacteria / 245 / Mn / dimer / Exce (0.000) / Secr (0.105) / None (0.3742)
P50058 / Leptolyngbya boryana / Cyanobacteria / 248 / Mn / dimer / Exce (0.000) / Secr (0.229) / None (0.2313)
ZP_01630049 / Nodularia spumigena / Cyanobacteria / 264 / Mn / dimer / Exce (0.000) / Secr (0.044) / None (0.0226)
ZP_00112125 / Nostoc punctiforme 4 / Cyanobacteria / 249 / Mn / dimer / Cypl (0.174) / Chlp (0.662) / None (0.1226)
YP_321963 / Anabaena variabilis / Cyanobacteria / 270 / Mn / dimer / Exce (0.222) / Secr (0.114) / None (0.2217)
NP_923628 / Gloeobacter violaceus / Cyanobacteria / 316 / Mn / dimer / Enre (0.222) / Mito (0.416) / None (0.2381)
YP_723986 / Trichodesmium erythraeum / Cyanobacteria / 254 / Mn / dimer / Exce (0.261) / Secr (0.280) / None (0.4769)
NP_680827 / Thermosynechococcus elongatus / Cyanobacteria / 240 / Mn / dimer / Exce (0.111) / Mito (0.979) / Mito (0.9575)
ZP_00514026 / Crocosphaera watsonii / Cyanobacteria / 254 / Mn / dimer / Enre (0.111) / Secr (0.109) / None (0.3859)
AAA22910 / Bacteroides fragilis / Bacteria / 193 / Cambialistic / dimer / Cypl (0.043) / None (0.071) / None (0.2862)
O15905 / Babesia bovis / Alveolata / 199 / Fe / dimer / Cypl (0.043) / None (0.126) / None (0.5890)
AAD03767 / Plasmodium falciparum / Alveolata / 198 / Fe / dimer / Cypl (0.174) / None (0.129) / None (0.5084)
AAL62028 / Neospora caninum / Alveolata / 201 / Fe / dimer / Cypl (0.000) / None (0.072) / None (0.0919)
AAO72229 / Toxoplasma gondii / Alveolata / 258 / Fe / dimer / Mito (0.391) / Mito (0.844) / Mito (0.9107)
CAA50204 / Entamoeba histolytica / Entamoebidae / 190 / Fe / dimer / Cypl (0.130) / None (0.102) / None (0.0944)
AAK01863 / Gonyaulax polyedra / Alveolata / 230 / Fe / dimer / Peox (0.111) / Mito (0.730) / Mito (0.7481)
AAQ14556 / Leishmania donovani chagasi / Euglenozoa / 208 / Fe / dimer / Cypl (0.000) / None (0.132) / None (0.0565)
EAN80448 / Trypanosoma brucei / Euglenozoa / 208 / Fe / dimer / Cypl (0.043) / None (0.065) / None (0.0805)
EAN87701 / Trypanosoma cruzi / Euglenozoa / 207 / Fe / dimer / Cypl (0.043) / None (0.075) / None (0.1596)
AAM22283 / Perkinsus marinus / Alveolata / 226 / Fe / dimer / Mito (0.565) / Mito (0.870) / Mito (0.9880)
AAO51246 / Dictyostelium discoideum / Mycetozoa / 243 / Fe / dimer / Cypl (0.304) / None (0.279) / Mito (0.8091)
EAY08700 / Trichomonas vaginalis / Parabasalidea / 195 / Fe / dimer / Cypl (0.087) / None (0.152) / None (0.2032)
AAC47735 / Tritrichomonas foetus / Parabasalidea / 195 / Fe / dimer / Cypl (0.174) / None (0.082) / None (0.1291)
CAJ02208 / Leishmania major / Euglenozoa / 230 / Fe / dimer / Mito (0.348) / Mito (0.560) / Mito (0.6459)
1 AlMnSOD, a MnSOD gene sequence from the microsporidian A. locustae was incomplete possessing only the portion encoding the C-domain. Thus the analysis of structure and location could not be carried out.
2 PnMnSOD2 and TpFeSOD, genes showed no targeting signal due to their incomplete sequences but are regarded here as mitochondria based on previous reports.
3 Although the metal cofactor of TtFeSOD and TpFeSOD has been reported as Fe, both resemble MnSODs based on structural criteria.
4 The MnSOD from cyanobacteria is predicted to possess chloroplastic targeting signal by TargetP program with plant datasets.
ND: no determined.
Table S2. Alignment of 72 SOD sequences using Muscle software with adjustments based on X-ray diffraction data for 3 Mn and 8 Fe SODs.
72 375
Dictyostelium discoideum ------MALVQKSMASEMQKRFIYTLPEIPYLN-SGIKNF-MSVPSLK-QHVKLHQEDIEKANNLV-QRTPWE------DTTINQAI------KQSFTSIEDAPFFESVSSHYNHSFFWRSISNIKE------TPSAHMKKALELDFGSIAGFQQKFSQSASALNVPGFTWLVFHD----KALRIITTFGSGSPLKLQNCH-----PILCLDLFEHAYVSDHGD-KNKYIANFWSCINWKFVEAKFLNALVSDREYKLRLESLVGKQSHAFEQFLDSQSNN
Trichomonas vaginalis ------MFSIVDVPFLQ-TGLGNF-LSAHAVNIHVTKHHQAYVDTANKLV--PESEFA------GQTLEQII------KTSTGAIFNNCSQ-HFNHSFFWMCFSYNIS------NPSDKVLDFFSKAFGSLDKFKDTFVNKASTIFGSGWCYLVLKPD---QTVEIIQYSNANNPIKDGYF------PILCIDTWEHAWYIDYENRKAEYFMRFWNYVSWDFVESRLQ--LAKII------
Tritrichomonas foetus ------MFSIEPVSFLE-SGLPNF-LTPHAVQIHVTKHHQGYVDMANKIV--PESEFK------GKSVEEII------QNASGPVFNNVAQ-HFNHSFFWKCLTATTQ------EVPAAVASFLSKHFESVDNFKAQFVQKASTVFGSGWCYLAVNKD---GSVSINQYSNALNPVKDGGV------PLLCVDTWEHAWYIDYENRKAEYFNKFWGVVNWNFVEENLK--KAKLI------
Leishmania major ------MFGRAPMKAATATAAVGFSCLCYHTLPHLRYPAELPTLGFNYKDGIQPV-MSPRQLELHYSKHHSAYVDKLNTLG-KGYE------GKTIEEII------LATTGINESKVMFNQAAQHFNHSFFWKCLSP----GGK--PMPKTLENAIAKQFGSVDDFMVSFQQAGVNNFGSGWTWLCVDPQT--KELLIDSTSNAGCPLTSGLR------PIFTADVWEHAYYKDFENRRADYLKELWQIVDWEFVCHMYE--RATK------
Bacteroides fragilis ------MTYEMPKLPYAN-NALEPV-ISQQTIDYHYGKHLQTYVNNLNSLV--PGTEYE------GKTVEAIV------ASAPDGAIFNNAGQ-VLNHTLYFLQFAPKPAKN----EPAGKLGEAIKRDFGSFENFKKEFNAASVGLFGSGWAWLSVDKD---GKLHITKEPNGSNPVRAGLK------PLLGFDVWEHAYYLDYQNRRADDVNKLWEIIDWDVVEKRL------
Perkinsus marinus ------MLSVAGHRFASLATTPVLRMGLARCFSSVTGPFQCPPLPYVK-NALEPH-MSAETLTYHHDKHHQTYVDTLNSIA-AENSTIA------SKTLEQII------KTETGKPFNQAAQ-VYNHTFFFNNLAP---NGGG--EPTGKIAELITRDFGSFEKFKEDFSAAAVGHFGSGWVWLIADD----GKLKIVQGHDAGNPIRESKT------PLMNIDVWEHAYYIDYRNARAQYVKNYWNLVNWDFVNDNVA--KAGI------
Gonyaulax polyedra ------MFAAAVSPIAAARASIVPRPHTRIMALQLPELPYSK-DALAPH-INQQTLEFHYGKHHNAYVTNLNKLI--EGTDLA------NAPLEEIV------KAAASNASKAGLFNNAAQ-VWNHTFYWHSMKP---GGGG--APHGKVAELIERDFGSYEEFATQFKAAGGGQFGSGWAWLVADKE---GKLKVFQTHDAGCPLTEGLT------PLLTCDVWEHAFYIDYKNDKAKYVQSFWGAVNWDFVASNFP--QGELRASKL------
Entamoeba histolytica ------MSFQLPQLPYAY-NALEPH-ISKETLEFHHDKHHATYVNKLNGLV-KGTEQE------HKTLEELI------KQKPTQAIYNNAAQ-AWNHAFYWKCMC----GCGV--KPSEQLIAKLTAAFGGLEEFKKKFTEKAVGHFGSGWCWLVEHD----GKLEIIDTHDAVNPMTNGMK------PLLTCDVWEHAYYIDTRNNRAAYLEHWWNVVNWKFVEEQL------
Leishmania donovani chagasi ------MPFAVQPLPYPH-DALASKGMSKEQVTFHHEKHHKGYAVKLNAAA-ESNSGLA------SKSLVDII------KSEKGPAFNCAAQ-IFNHDFFWRCLSP---RGGS--KPHGEIASAIVDSFGSFASFKKEFTDAANGHFGSGWAWLVKDKSS--GKLKVLQTHDAGCPLTEPNLV-----PILTCDIWEHAYYIDYRNDRASYVNAFWNMVNWSHANRCYR--AAGGSHYVNSDL------
Trypanosoma brucei ------MAFSIPPLPWGY-DGLAAKGISKEQVTFHYDKHHMGYVTKLNAAA-KSNPALA------AKSVEEII------RTEKGPIFNLAAQ-IFNHNFYWESMSP---NGGG--EPSGKLAEAIRASFGSFAKFKEEFTNAAVGHFGSGWAWLVQDTTT--KKLKVFQTHDAGCPLTEADLK-----PILACDVWEHAYYIDYKNDRPAYVQTFWNVVNWDHAENQFT--RKRNPGAPHSDL------
Trypanosoma cruzi ------MVFSIPPLPWGY-DGLAAKGLSKQQVTLHYDKHHQGYVTKLNAAA-QTNSALA------TKSIEEII------RTEKGPIFNLAAQ-IFNHTFYWESMCP---NGGG--EPTGKLADEINASFGSFAKFKEEFTNVAVGHFGSGWAWLVKDTNS--GKLKVYQTHDAGCPLTEPNLK-----PLLTCDVWEHAYYVDYKNDRAAYVQTFWNVVNWKNVERRLSETGYVHLRSYL------
Toxoplasma gondii ------MFAAVARRTATGTLPGAVTLFGRKSFEGYYRDYPLHPRNFGVMASLFSTGKFELPALPYDY-NALEPY-ISAKTLKFHHDKHHATYVKNLNELI--KGTDYE------NQKLEDII------RNADGPTFNNAAQ-AWNHTFFWNSMKP---NGGG--EPTGLVKEQIDRCFSSFSKYKEEFSEKATKHFGSGWAWLVWCNEK--RRLEVIETHDAGNPITMKKW------PIITCDLWEHGYYLDRQNDRGAYVKSWWSVVNWDFANEKLE--KAMRGDPIDETVGGGQ------
Neospora caninum ------MVFTLPPLPYAY-DALAPH-ISADTLQFHHGKHHAGYVAKLNGFI-EGTAFA------GKTLEEVV------RSSTGAIFNNAAQ-VWNHTFYFNSMKPPTAGGGG--KPTGRLLDEINKEFTSVEKFKEEFSKVAAGHFGSGWAWLVWDKQG--KKVGIEQTHDAWTPITEQMKV-----PLLCCDVWEHAYYLDRKNDRPAYIKAWWEVVNWDFASKNLD--NALK------
Babesia bovis ------MAFKLPALPYGM-RELIPH-ISEETLSFHYGKHHAGYVNKLNSLI-KGTPLE------SCTIEELI------LGQTGAVFNNAAQ-IWNHTFYWNSMGP---NCGG--EPTGPIRKKIEEKFGSFSAFKTDFSNLLAGHFGSGWGWLVLKDD---GTADIVQTHDAGSPLKENLGR-----PLLCCDVWEHAYYIDYKNDRLSYINSWWNLVNWDFANKNLE--APFKWS------
Plasmodium falciparum ------MVITLPKLKYAL-NALSPH-ISEETLNFHYNKHHAGYVNKLNTLI-KDTPFA------EKSLLDIV------KESSGAIFNNAAQ-IWNHTFYWDSMGP---DCGG--EPHGEIKEKIQEDFGSFNNFKEQFSNILCGHFGSGWGWLALNNN---NKLVILQTHDAGNPIKDNTGI-----PILTCDIWEHAYYINYRNDRASYVKAWWNLVNWNFANENLK--KAMQK------
TtFeSOD ------MLNKVVKRLFSYKSTIPATLPQLKFEY-NELEPV-LTANLLKFHHGKHHQAYVNNLNAIY-EQISDAQK------KNDVHKIA------QLQSGLRFNLGG-HINHAIYWDNLAPVSRGGGVFPDQNSPLTKAIQEKWGSYENFIQIFNGRTAAIQGSGWGWLGYDTVS--KSLKMFELGNQDMPEWNSVI------PLLTIDVWEHAYYLDYQNLRPKYLTEIWKVVNWQEVERRYL--DAIKA------
TpFeSOD ------LNYEY-SDLEPV-LSAHLLSFHHGKHHQAYVNNLNATY-EQIAAATK------ENDAHKIA------TLQSALRFNLGG-HVNHWIYWDNLAPVKSGGGVLPDEHSPLTKAIKEKWGSYENFITLFNTRTAAIQGSGWGWLGYDTVS--KSLRLFELGNQDMPEWSSIV------PLLTIDVWEHAYYLDYQNLRPKYLTEVWKIVNWREVEKRYL--QAIE------
Oryza sativa ------MALRCVASRKTLAAAALPLAAAAAARGVTTVALPDLPYDY-GALEPA-ISGEIMRLHHQKHHATYVANYNKAL-EQLDAAVA------KGDAPAIV------HLQSAIKFNGGG-HVNHSIFWNNLKPISEGGGE--PPHAKLGWAIDEDFGSFEALVKKMSAEGAALQGSGWVWLALDKEA--KKLSVETTANQD-PLVTKGANLV---PLLGIDVWEHAYYLQYKNVRPDYLSNIWKVMNWKYAGEVYE--NATA------
Arabidopsis thaliana ------MAIRCVASRKTLAGLKETSSRLLRIRGIQTFTLPDLPYDY-GALEPA-ISGEIMQIHHQKHHQAYVTNYNNAL-EQLDQAVN------KGDASTVV------KLQSAIKFNGGG-HVNHSIFWKNLAPSSEGGGE--PPKGSLGSAIDAHFGSLEGLVKKMSAEGAAVQGSGWVWLGLDKEL--KKLVVDTTANQD-PLVTKGGSLV---PLVGIDVWEHAYYLQYKNVRPEYLKNVWKVINWKYASEVYE--KENN------
Tribolium castaneum ------MFALRRLATAPLAGSRAAHSLPELPYAY-EALEPV-ISRDIMCLHHSKHHQTYVTNLNAAE-EKLKAALS------KGDISTAI------SLEPALRFNGGG-HLNHSIFWQNLSPE----ST--QPSDALKKAIEDSFGGVQQLKDQLSASSIGVQGSGWGWLGYCSKS--GKLKIATCANQD-PLQATTGLV----PLLGIDVWEHAYYLQYKNVRADYVKAIFEIVNWKDVSQRFE--KAKSC------
PnMnSOD2 ------RAIATVTLPDLPYDF-GALEPS-ISGQIMEIPHQKHHRTYVNNYNATL-EQYAEAES------KGDHAKLL------TLLPALKFNGGG-HVNHSIFWTNLAPKNQGGGG--EPEGELRKAIDQEFGSFENFKKTMATKSAAVQGSGWGWLGYSPET--KRVGVVTSPNQDPCSTTGYV------PLLGIDVWEHAYYLQYKNVRPDYLNAIW------
Apis mellifera ------MFAARRILFSNTVKDTFTRTKHTLPDLPYDY-KALEPI-ISAEIMQLHHSKHHATYVNNLNVAE-EKMKEAVA------KGDVNTQV------ALSPAIKFNGGG-HLNHSIFWCNLSP----NGG--KPDAALLNQIKKDFGSIEEMKKRLSENTIAIQGSGWGWLGYCQKS--KSLRIATCANQD-PLQATTGLI----PLFGIDVWEHAYYLQYKNVRPDYVKAIFDVVNWNDVNSRYK--KAIGS------
NvMnSOD2 ------MLLARVARLSSRKAVLPVALVRAKHTLPDLPYDY-DALEPT-INTEIMRLHHSKHHATYVNNLNIAE-EKCLEAQA------KGDVATAI------ALQPAVKFNGGG-HLNHSIFWTNLSP---NGGG--EPTGELMEAIKRDFGSFENFKERFNAATIAVQGSGWGWLGYDKVN--KRLAIATCFNQD-PLQPTTGLV----PLLGIDVWEHAYYLQYKNVRPDYVKAIYDVINWTNVAERLQ--AASS------
Danio rerio ------MLCRVGYVRRCAATFNPLLGAVTSRQKHALPDLTYDY-GALEPH-ICAEIMQLHHSKHHATYVNNLNVTE-EKYQEALA------KGDVTTQV------SLQPALKFNGGG-HINHTIFWTNLSP---NGGG--EPQGELLEAIKRDFGSFQKMKEKISAATVAVQGSGWGWLGFEKES--GRLRIAACANQD-PLQGTTGLI----PLLGIDVWEHAYYLQYKNVRPDYVKAIWNVVNWENVSERFQ--AAKK------
Xenopus laevis ------MLCRLSVCGRGRMRCVPALAYSFCKEKHTLPDLPYDY-GALQPH-ISAEIMQLHHSKHHATYVNNLNITE-EKYAEALA------KGDVTTQV------SLQAALKFNGGG-HINHTIFWTNLSP---NGGG--EPQGELLDAIKRDFGSFEKFKEKLNTVSVGVQGSGWGWLGYNKDS--NRLQLAACANQD-PLQGTTGLI----PLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYQ--ASKK------
Gallus gallus ------MLCRLASAGRSRAALVAPWGCLVARQKHTLPDLPYDY-GALEPH-ISAEIMQLHHSKHHATYVNNLNVTE-EKYKEALA------KGDVTAQV------SLQPALKFNGGG-HINHTIFWTNLSP---SGGG--EPKGELMEAIKRDFGSFANFKEKLTAVSVGVQGSGWGWLGYNKEQ--GRLQIAACANQD-PLQGTTGLI----PLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVSQRYE--SCRK------
Homo sapiens mitochondria ------MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDY-GALEPH-INAQIMQLHHSKHHAAYVNNLNVTE-EKYQEALA------KGDVTAQI------ALQPALKFNGGG-HINHSIFWTNLSP---NGGG--EPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKER--GHLQIAACPNQD-PLQGTTGLI----PLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYM--ACKK------
Yarrowia lipolytica ------MLARNTLRQTIRKAQPMAGRTLTTAVLPKLDYDF-GALEPF-VAGQINEIHYTKHHQTYVNSFNAAS-EQLASAEK------DGDLKKIV------ELQNLINFHGGG-HINHSLWWKNLAPKGQGGGELPSPDSPLGKAIKEQWGDANKLIETTNAALAGVQGSGWAWIVKNKLT--GDIEVITKQNQD-PVAGNHI------PLVGIDAWEHAYYLQYKNVKADYFKAIWNVINWQEAEKRFL--GEAKFVNGQ------
Candida albicans ------MFSIRSSSRVLLKASSATTRATLNAAASKTFTRSKYSLPELDYEF-SATEPY-ISGQINEIHYTKHHQTYVNNLNASI-EQAVEAKS------KGEVKKLV------ALQKAINFNGGG-YLNHCLWWKNLAPVSQGGGQPPSEDSKLGKQIVKQFGSLDKLIEITNGKLAGIQGSGWAFIVKNKANG-DTIDVITTANQDTVTDPNLV------PLIAIDAWEHAYYLQYQNVKADYFKNLWHVINWKEAERRFE--F------
Debaryomyces hansenii ------MLSSTLRNTVSRGFSNTAAKSTIGAVRTKISLPDLDWDF-GALEPH-ISGQINELHYTKHHQTYVNGYNTAI-EQQAEAKS------KGEVKKTI------ELQKNINFHGGG-YTNHCLFWKNLAPEKQGGGEPPSSDSELAQRIAAQYGSIENLQSIANAKLAGIQGSGWAFLVKNKENG-GSLDVVTTYNQD-TVTGPLV------PLLAIDAWEHAYYLQYQNVKADYFKAIWKVINWKEAEKRYL--VN------
Saccharomyces cerevisiae ------MFAKTAAANLTKKGGLSLLSTTARRTKVTLPDLKWDF-GALEPY-ISGQINELHYTKHHQTYVNGFNTAV-DQFQELSDLLAKEPSPANARKMI------AIQQNIKFHGGG-FTNHCLFWENLAPESQGGGE--PPTGALAKAIDEQFGSLDELIKLTNTKLAGVQGSGWAFIVKNLSNG-GKLDVVQTYNQD-TVTGPLV------PLVAIDAWEHAYYLQYQNKKADYFKAIWNVVNWKEASRRFD--AGKI------
Kluyveromyces lactis ------MFAKSVARRALQLQPTLLAKRTKVSLPELDWDF-GALEPH-ISGQINELHYTKHHQTYVNGFNAAV-EQFDELKS------KLDNDPAV------AKQIVAVQQNLKFHGGG-YVNHCLFWKNLAPTSQGGGE--APTGALAKQIETQFGSLDNLISLTNAKLAGVQGSGWAFIVKNVENG-NQLEVVQTYNQD-TVTGPLK------PLVAIDSWEHAYYLQYQNQKANYFKAIWNVINWKEAAKRFE--AAA------
Neurospora crassa ------MSASLFRATPVARSALRAASAVKPAATFVRGKATLPDLQYDY-GALEPY-ISSKIMELHHSKHHQTYVNGLNSAL-TTIAEAES------KGDFTKAA------SVAPLLNFHGGG-HLNHTLFWENLAPASREGGG--SPDGALGKAITTEFGSFESFVKQMNAALAGIQGSGWAWLAKDKAAN-GKLALITRANQD-PVTGNYV------PLLGIDAWEHAYYLQYENRKAEYFEAIWNVINWKTVAKRFE--A------
Magnaporthe grisea ------MASLIRTTPAVRGALRSRAFKPAAMASTSFVRGKATLPDLKYDY-GALEPY-ISAEIMELHHSKHHNTYVQGYNSAV-QAIAEAP------TPQAAAAV------APLLNFHGGG-HVNHSLFWENLAPASREGGG--EPDGKLRAGIDATFGSFETFTKQMNTTLAGIQGSGWAWLAKDKTT--GALSIVTRANQD-PVAGNLA------PLLGIDAWEHAYYLQYQNRKAEYFSAIWNVINWKTVGNRFD--KS------
Gibberella zeae ------MASALLRSTPALRAGLRARSTPLAAMATTSFVRGKATLPDLPYDY-GALEPY-ISGQIMELHHSKHHQTYVTGFNNAT-DAIAEANH------KGDAKAAA------AQAPLLNFHGGG-HVNHSLFWENLAPNGKGGGG--EPEGKLLTSINEDFGSFEAFKKQTNATLAGIQGSGWAWLVKDKNA--GTLSIVTRPNQD-PVTGNLE------PLLGIDAWEHAYYLQYQNRKAEYFSAIWDVINWGTVAKRFE--K------
Cordyceps militaris ------MASSLLRTTPAVRAGLRAVSKPAVAMASTSFVRGKATLPDLPYDY-GALEPA-ISGKIMELHHSKHHQTYVNGFNAAV-EALSEAQA------KNDSKAAA------AQAPALNFHGGG-HINHSLFWENLAPNGKGGGG--EPAGKLSTAITEDFGSFDNLKKQTNAALAGIQGSGWAWLVKDKTS--GTLSVVTRPNQD-PITGNLE------PLLGIDAWEHAYYLQYENRKVEYFSAIWDVINWGTVAKRFE--K------
Aspergillus fumigatus ------MAASLIRTSARTALRSGASAAPRAATSLTFARGKATLPDLSYDY-GALEPS-ISGKIMELHHKNHHQTYVNSYNQAI-EQLQEAQA------KNDIASQI------ALKPLINFHGGG-HLNHTLFWENLAPKSSGGGE--PPSGALAKAIDETYGSLENFQGKMNTALAGIQGSGWAWLVRDKQT--GHIGIKTYANQD-PVVGQFQ------PLLGIDAWEHAYYLQYQNRKAEYFKAIWEVINWKAVEKRFS--A------
Paracoccidioides brasiliensis ------MTTSLFRSTVGGILRVGAARMSGAAGARLITTKATLPDLSYDY-GALEPS-ISGKIMELHHKKHHQTYVNSYNDAV-EKLAAAQE------KADIKSQV------AFQALTNFHGGG-HVNHTLFWENLAPKSAGGGE--PPAGSLGKAVDDAFGSYDRFKSTFNTALAGIQGSGWAWLVEDKQT--GKIAIKTYANQD-PVIGQFK------PLLGIDAWEHAYYLQYENRKAEYFNAIWNVVNWKAAEKRFA------
Blumeria graminis ------MVLKYNIPPLPYAY-DALEPH-ISSQIMNIHHTKHHQTYVNNLNNAL-ETLDALTK------SSDIVSHL------HLQAAISFNAGG-HINHTLFWENLSPATSTTAQ-ISSGPKISAALAARWGSVERFQESFTTVLLGLKGSGWGWLVQDIES--GTLEIITTKDQE-IVPGNKK------PLLGIDFWEHAYYLQYLNNKAAYAKEIWKVINWAKVEERYV--GSPENVFGNLKLLKGEL------
Schizosaccharomyces pombe ------MLRFLSKNSVAAIRNVSIARGVHTKATLPPLPYAY-NALEPA-LSETIMKLHHDKHHQTYVNNLNAAQ-EKLADP------NLDLEGEV------ALQAAIKFNGGG-HINHSLFWKILAPQKEGGGK-PVTSGSLHKAITSKWGSLEDFQKEMNAALASIQGSGWAWLIVDKD---GSLRITTTANQDTIVKSK------PIIGIDAWEHAYYPQYENRKAEYFKAIWNVINWKEAESRYS--NR------
Penicillium chrysogenum ------MTSQTHTLPPLPYAY-DALEPV-ISKQIMELHHQKHHQTYINNLNAAL-SAQASATA------SNDVPTLI------SLQQKLRFNGGG-HINHSLFWKNLTPPGTPAND-IAGAPALREAIVSRWGSHEAFVKAFGAELLGLQGSGWGWLVSKGGAK-GRLEIVTTKDQD-PVNAPDV------PVFGVDMWEHAYYLQYLNNKAGYVEGIWKIIHWAEAEKRYT--AGVENPLKL------
Aspergillus fumigatus ------MSQQYTLPPLPYPY-DALQPY-ISQQIMELHHKKHHQTYVNGLNAAL-EAQKKAAE------ANDVPKLV------SVQQAIKFNGGG-HINHSLFWKNLAPEKSGGGK-IDQAPVLKAAIEQRWGSFDKFKDAFNTTLLGIQGSGWGWLVTDGPK--GKLDITTTHDQD-PVTGAA------PVFGVDMWEHAYYLQYLNDKASYAKGIWNVINWAEAENRYI--AGDKGGHPFMKL------
Magnaporthe grisea ------MASSTTYKLPELPFAY-DALEPH-ISKQIMELHHSKHHAAYVANLNKAL-ETYA------SGQLADRV------ATLAAINFNAGG-HINHTLFWENLTPASSPDADAATSAPSLVTQIEKQWGSLDQFKAEFSAELLGIKGSGWGWLVKEKSAA-GALVVVTTKDQD-SVPSGI------PVFGVDMWEHAYYLQYLNGKAAYVENIWKVINWKTAEKRFA--SPGRSEVFKALHASL------
Gibberella zeae ------MFPLLPLLLLLTSPTSSSSVSAKPDLASDNNILPDNIPTYSAMSVGTYSLPALPYAY-DALEPS-ISAQIMELHHSKHHQAYVTNLNVAL-KNYATATS------SSDIAGQI------ALQSAIKFNGGG-HINHSLFWENLCPSSSPDSK-PDSAPTLGAEISKTWGSIEAFQETFKKTLLGLQGSGWGWLVKDT----QGLRIVTTKDQD-PVVGGEV------PIFGVDMWEHAYYLQYLNGKAAYVDNIWNVINWKTAEARFT--GSREDAFKVLRASI------
Neurospora crassa ------MTGNATTMAGLATYSLPQLPYAY-NALEPY-ISAQIMELHHSKHHQTYVTNLNNAL-KVHVAAIA------SSDIPAQI------AQQPAIKFNGGG-HINHSLFWKNLAPAETPETNYSKAAPSLAAEIEKTWGSFDEFKKAFSAALLGIQGSGWGWLVKESTAEKGRLRIITTKDQD-PVVGGEV------PVFGVDMWEHAYYLQYLNGKAAYVENIWKVINWKTAEERFQ--GSREDAFADLKALL------
NbMnSOD2 ------MAFSLPELSFNF-EDLEHF-IDRETMKAHYTKFHGSYVKILNWTV-KKYKIE------EECLVTLLSNIKKS--TKYDDFVKESLIYNGGG-HLNHSLFWCSMSP--DSEAE--DIKQDLAVDLRSDFGSITKFCDKFGDEAHSFQGSGALWLIYDRTS--ETLKIKSTKNNDNPIMFNENHI----PLIGLDLWEHAYYHAHKNKRYNYVLNWEKVFNWKFLNSAYEKYVVEGKKFEMTPDGKFNV------
NbMnSOD1 ------MVFSLPNLPYKY-EDLEPH-IDAETMNTHHTKHHVTYINKLNTTV--KDNDIK------EDCLATLLTEIDKN--TTYSGEVKKMLINNGGG-HLNHSLFWCYMTP--KSDIT--DIGEGLMKEMDKDFESFDNFKKTFDSNAVALFGSGWVWLCFDKSS--KKLTIISSGNQDNPLMYDANHI----PLLGLDVWEHAYYLRYKNKRPEYVSNWWNVVNWKFVNCAYESFVVKGKKFEMTPDGTLKL------
EcMnSOD ------MEFKLPELDYPY-DALEPI-IDEETMRTHHSKHHQAYINSLEKTL--RSNSIK------GKSLYYYVTKGRGI--RGVKSSARRDLLNFSGG-HYNHSLFWKMMCP--PGTSG--PMSPRLLEYIERSFGSQEKMVKEFSNAAVSLFGSGWVWLCYRQSE--GILVIRKTYNQDAICMKTSSTV----PILGLDVWEHAYYLKYKNVRSEYVANWWKVVNWGLVSRLFEEIALENKKLCVMSDGSIKFD------
Crocosphaera watsonii ------MKISNRANFLFFGIVLLICCLWGNLNNAPIYSAVPSYTTRVSQIAQTSNGDFVQAPLPYDY-NALEPF-IDAKTMEIHYTGHHGGYLKKFKKTL-EKHPELK------GKTLEQIL---GNI--DNIPSDIRTSIRNNGGG-HYNHTVFWAIMTP---NGGG--EPNGEFATAINNSFGSFEEFKAQFKAESFKRFGSGWVWLVRDKN---GRLEITSSPGQDNPIMEGKY------PILGTDVWEHAYYLNYKNSRGSYVDAWWNTINWDEVNARFK--ESQTV------
Candidatus Nitrosopumilus maritimus ------MVQYELPRLPYGY-DELEPY-IDTETMKIHHQKHHQSYVDGLNKSLIEIGSASH------PQYITSIL---SDL--KAIPESGRNKINFFGGG-FENHRLFWEIMTP---NGDG--SPGGKIEDAIDVYFDSFDNFKEIFSNTAIGIQGSGWCWLVFNSTY--SKIEILSTENQTSPWTLQKI------PLLGLDVWEHAYYLKYQNRRPDYVKEWWNIVNWDYVENRFS--ELVG------
Trichodesmium erythraeum ------MNINRRHFLLLLSTTLGFMTVSSCKISLDNSSGSVIESTLPNDTKFSENLGVFQLTPLPYGY-DALEPY-IDKETMGFHYGKHYAGYIKKLNGAI-AQYPKLK------EFTAEELL---LGL--NSLPQDIRTAVRNNGGG-YVNHKMFWEIMAP---NGGG--KPEGKIAVAIDESFGNFDGFKERFNAAGAARFGSGWVWLVLGND---GKLKVISTLNQDSPFLTGNY------PIMGNDVWEHAYYLRYQNRRRDYLNAWWNIVNWPEVNRRYE--LALR------
Thermosynechococcus elongatus ------MRLTRRQTLGLLGVSAATLWLRSRPAVAAGEPSEIYTLPPLPYDY-DALEPV-IDAETMRLHHDKHHAAYVNTLNKAL-TPYRQWH------GLAIETLL---RHL--HQLPTAIQSTVRNHGGG-HANHSLFWQSMAP---NGGG--EPAGDLAIAIENTFGSFPEFQAQFQQAGLRHFGSGWVWLVLTPQ---GNLTITTTLNQDSPLMQGQV------PILGNDLWEHAYYLTYRNRRDQYLEAWWQVVNWPWVSDRYA--QMRELLA------
Gloeobacter violaceus MVLGVLLTGKARYSPLLKAACNFPAGRAPCGSPEAKTGSLCQEFVSMKCSVSTLVGTLCLTAAALLSTAAEAQINIPTPTITPPTVTVPSVQPPSVTMPSATQASTTASGYPYKLAPLPYDY-SALEPY-IDAETMKLHHDKHHQAYVDNLNKAL-EKYPDLQ------KKSPEQLL---RDL--KQVPEDTRAAVRGNGGG-HVNHTMFWEIMKP---KGGG--EPAGPIAAAIRTNFGSFDAFKTQFNEAGTKRFGSGWVWLVSNPG---GKLEIVSTANQDSPIADGKY------PIMGNDVWEHAYYLKYQNRRADYLSAWWNTLNWDEINRRFQ--KASTGL------
Leptolyngbya boryana ------MQTTFRRILILFVGLLVPLFFACQSNSQVDAAPSAAPQLSASPAKLDPLPYDY-AALEPY-IDAQTMRLHHDKHHATYVNNINETL-KAYPDLQ------KQSVDSLI---QNL--NQVPEAIRTKIRNNGGG-HVNHTMFWQIMAP---KAGG--TPTGAVAKAIDQTFGSFDAFKQQFNKAGADRFGSGWAWLVSDRQ---GKLSITSTANQDNPLMSNPNAY----PILGNDVWEHAYYLKYQNRRAEYLTNWWNVVNWQAVNQRYA--QAQRK------
Anabaena variabilis ------MKSSLWQRFIGFFMVILVTSMLYACQPQEAANSLPTNVASPVQTTATATDKRSIGFLDRPLGTNPAELPPLPYGY-DALEKA-IDAETMKLHHDKHHAAYVNNLNNAL-KKHPELQ------NSSVEALL---RDL--NSVPEDIRNTVRNNGGG-HLNHTIFWQIMSP---NGGG--QPTGDIAQEINQTFGNFEEFKKQFNKAGGDRFGSGWVWLVRNPQ---GQLQIVSTPNQDSPIAEGSY------PIMGNDVWEHAYYLRYQNRRPEYLNNWWNVVNWSEINKRAQ--ASRQSNS------
Nodularia spumigena ------MSKFIREKIAFVLTGMLLAMMLISCQPVPVAESQSPQGTPIAATFPPVASPDGELSASPAKLPLLPYDY-AALGKA-IDAETMKLHHDGHHASYVNNLNDAL-KQAPDLQ------KNSVEALL---RDL--NNVPENIRTKVRNNGGG-HLNHTIFWQIMSP---EGGG--EPIEEIAAEINQTFGSFEEFKKQFNAAGGDRFGSGWVWLVRNPA---GELQIVTTANQDNPIMDGAY------PIMGNDVWEHAYYLRYRNRRAEYLKNWWNVVNWPEVNKRSL--VNLQKGT------
Nostoc punctiforme ------MLAILLISHQPVLIAEAQSSTPTPTPASSFPPVAYPNRALSAFPAQLPPLPYGY-GALGKA-IDAETMKLHHDVHHASYVKNLNDAL-KQYPDLQ------KRSVEALL---VDL--KSVPEDIRTKVRNNGGG-HLNHTIFWQLMSP---DSGG--QPTGAIAQEINQTFGSFDAFKKQFNAAGAARFGSGWVWLVRNPQ---NQLQIVTTANQDNPIMDGFY------PIMGNDLWEHAYYLKYRNRRAEYLNNWWNVVNWPQINRRSQ--LSSQQPR------
Cenarchaeum symbiosum ------MGEYTLPEMPYAY-DALEPH-IDARTMEIHHTKHHQTYTTKLNAALKDCPADIQ------GKDVTEIL---ANI--GQVPEAQRKAINFNAGG-YDNHRLFWNNMKP---NGGG--EPGGAVADAINSSFGSFADFKEKFSSTTAVIQGSGWGWLVYNPSS--SKVEYKAMPNQTSPRTEKLV------PLLGCDVWEHAYYLKYQNKRPDYISAWWNVINWDEVESRLS--KAK------
Stigmatella aurantiaca ------MRLFLMTTLTAVLTGSAALAQGATPPAKPAAAATKPAPSAPFTLDDLPYAY-EALEPV-IDAETMRIHHGAHHKAYVDNLNKAV-AANSALA------GQSLDKIL---ANV------SRYDAAVRNNAGG-HYNHTLFWKLMAP--PGKGG--APSKALADQITKDFKSIDEFKKAFAEAGTKRFGSGWAWLVWTG----DKLQVGSTPNQDNPLMDASELKGT--PVIANDVWEHAYYLKHRNKRDSYLSGWWEVLNWNEANRLFD--EARAAKKK------
Wolinella succinogenes ------MLKSTVSSFKSLLVASFLTLTASVAMANPFTLAPLPYAF-DALEPA-IDKETMQIHHGKHHQAYVNNLNAQV-ATYPELA------KMSLEQIM---AKI------SSYNMAVRNNGGG-HYNHALFWTLMAP--VGKGG--TPSSALAKAIDRDFGSLDKMKEAFEKAGATRFGSGWAWVVVTAD---KKLAITSTPNQDNPLMDIAEVKGT--PILALDVWEHAYYLKYQNRRGDYLKEWWKVVNWNEVNKLFD--QAVK------
Hyphomonas neptunium ------MYRTLAIATAALGAAACTTVTSTALASEPAAPVATETSAAAITYTLPDLPYAY-DALVGS-IDAQTMEIHHSKHHAGYVNNLNAAL-ADDAALA------SQPLEDLL---ANV------STHSTAIRNNGGG-HYNHTLFWTLMAP--AGEGG--APSTELLADIDATFGSLDAFKTEFNKAATSQFGSGWAWLVVTD----DGLKITSTPNQDNPLMDVAAVQGE--PLLAVDVWEHAYYLSYQNRRADYLKTWWNVVNWNEVNSLYE--AAKS------
PnMnSOD1 ------MSFELPKIAYDY-NALEPF-VDTDTMNIHHTKHHQAYVNNINNYISSDKGTALK------GKSILEVV------QSATEAPVRNNGGG-HYNHSLFWTWMTS---PGSTNTAPHGALKTRIEEDFGSLDQMKQEFNAAASSRFGSGWAWLGVKAD---GKLAITSTPNQDNPLMPGVDQPLI--PILGLDVWEHAYYLKYQNRRPEYISAFWNVANWDKVVEYYDEFASKGKPVDI------
Chlamydomonas reinhardtii ------MAQALPPLPYDY-GSLEPH-VDATTMNIHHTKHHQTYVNNLNAAL-DKFPELK------DLGLVDLN---KAVGTDKLPKDVATVIRNNGGG-HYNHSFFWKVMTNPSNTNG----PNGDVKAAIEASFGSVDEMKAKFNAAAAGRFGSGWAWLSVKPD---GSLSIDSTPNQDNPLMTALPDVAGGIPLLGLDVWEHAYYLKYQNRRPEYIAAWWNVVNWEQVAENYK--AAQAGTVPL------
Yersinia enterocolitica ------MSYSLPSLPYAY-DALEPH-FDKQTMEIHHTKHHQTYVNNANTVL-ESFPELA------KFSVEDLI---KDL--DKVPAEKRTFMRNNAGG-HANHSLFWKGLKL-----GT--TLTGDLKAAIERDFGSVDSFKEKFEAAAATRFGSGWAWLVLKDD---GKLAVVSTANQDSPLMGEAVSGASGFPIVGLDVWEHAYYLKFQNRRPDYIKAFWNVVNWDEAAARFA--QAK------
Escherichia coli ------MSYTLPSLPYAY-DALEPH-FDKQTMEIHHTKHHQTYVNNANAAL-ESLPEFA------NLPVEELI---TKL--DQLPADKKTVLRNNAGG-HANHSLFWKGLKK-----GT--TLQGDLKAAIERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKG----DKLAVVSTANQDSPLMGEAISGASGFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFA--AKK------
Tatumella ptyseos ------MSYSLPSLPYAY-DALEPH-FDKETMEIHHTKHHQAYVNNANAAL-EG-TEFA------SLPVEELI---TKL--DQVPADKKTALRNNAGG-HANHSLFWKGLKT-----GT--TLQGELKAAIEKDFGSVENFQAEFEKAAATRFGSGWAWLVKQG----DKLAVVSTANQDSPLMGEAVSGTSGFPILGLDVWEHAYYLKFQNKRPDYIKAFWNVVNWDEAAARFA--SAK------
Comamonas testosteroni ------MSYTLPPLAYDY-AALEPH-IDAQTMEIHYSRHHQTYVNNLNAAL-EG-SEYA------QWSLDDLL---AKV--ESLPQTLRQAVRNNGGG-HANHSLFWQVMSP---GGGG--LPQNALAAAIDKDLGGFENFKTEFTKAAISRFGSGWAWLSVDRS---GKLHVESSANQDNPLMLGIGSGNT--PILGLDVWEHAYYLKYQNRRPEYIAAFYNVVDWNEVARRFA--AATQAVRS------
Delftia acidovorans ------MSNYTLPPLNYAY-DALEPH-IDAQTMEIHYTKHHQAYVNNLNAAL-KDTPHG------SQPVEQLI---ADL--SLLPEGVRGAVRNNGGG-HANHSLFWTVMAPVDQGGGG--APSGELAQAIDRELGGFDAFKAAFTQAALTRFGSGWAWLVVGKD---GKLAVESSGNQDSPLMAGIGSGAT--PILGLDVWEHAYYLKYQNRRPEYIAAFYNVVNWAEVARRYA--AARG------
Methylobacillus flagellatus ------MAYTLPPLDYAY-TDLEPH-IDAQTMEIHHTKHHQTYINNVNAAL-EGTPFA------DEPIEALV---AKL--DSLPENLRGPVRNNGGG-HANHSLFWKVMSP---NGGG--EPTGALADAIKSDIGGFDTFKEAFTKAALTRFGSGWAWLSVTPE---KKLIVESSANQDSPLSHGNT------PILGLDVWEHAYYLRYQNRRPEYIGAFFNVINWDEVARRYQ--EAISK------
NvMnSOD1 ------MAFELPKLPYAY-DALEPH-IDARTMEIHHSKHHNGYTTNLNNAI-SG-TDLE------GKSIENIL---INL------DLNNGAVRNNGGG-YYNHNLFWTVMSP---NGGG--NPTGELAAAIDAAFGSFEAFKTEFSKAAATRFGSGWAWLCVHKG---GKLEVCSSANQDNPLMPGIGCGGT--PILALDVWEHAYYLNYQNRRPDYVEAFFNVIDWTEVTRRFA--TEK------
Blastopirellula marina ------MAYTLPELPYAY-DALEPS-IDAKTMEIHHTKHHQAYITKVNDAI-KG-TALE------SKTIEELV---SDL--SAVPENVRGAVRNNGGG-HANHSLFWTIMKP---NGGG--APTGALAAAIDAELGGFEKFKEDFSKAAATRFGSGWAWLSVDG----GKLLVESTPNQDTPLMEGRT------PILGLDVWEHAYYLNYQNRRPDYVSAFFNVINWDEVSKLYA--AATA------
Planctomyces maris ------MSYSLPDLPYAY-DALEPH-IDAKTMEIHHTKHHQAYISKANAAL-EGHSDLA------AKSIEDLM---SDL--SAVPEDIRGAIRNNGGG-HANHSLFWTVMSP---SGGG--APSGDLAADIDSTFGSFDAFKEQFSNAAATRFGSGWAWLSVDG----GKLVVESTPNQDTPLSEGHT------PILGLDVWEHAYYLNYQNKRPDYISAFFNVINWDEVAKRYA--AAKG------