Supplementary Material

Supplementary Table S1. Sole-carbon-source utilization of strain JL1095T and the type strain of Stappia stellulatum IAM 12621T

Biolog GN2 carbon sources / 1 / 2
Tween 40, Tween 80, L-arabinose, methyl-pyruvate, β-hydroxy butyric acid / + / +
D,L-Lactic acid, acetic acid, urocanic acid, α-hydroxy butyric acid, γ-hydroxy butyric acid / + / -
L-Proline, glycerol, α-keto butyric acid / W / W
D-Fructose, L-fucose, D-galactose, α-D-glucose, D-mannose, L-serine, D-sorbitol, D-gluconic acid, α-keto glutaric acid, succinamic acid, L-glutamic acid / W / -
L-Leucine, α-keto valeric acid, L-alaninamide, mono-methyl-succinate / - / W

1, JL1095T; 2, Stappia stellulatum IAM 12621T.

+, positive; -, negative; W, weak.

Supplementary Table S2. Cellular fatty acid components and quantities (%) of strain JL1095T and the type strain of Stappia stellulata IAM 12621T

Fatty acids / 1 / 2
C12:0 / 3.15 / -
C14:0 / 1.96 / -
C16:0 / 11.09 / 6.97
C18:1 ω9c / 1.02 / 1.42
C18:0 / 12.41 / 4.20
C18:1 ω7c 11-methyl / - / 13.68
C19:0 ω8c cyclo / 20.23 / 3.21
C18:0 3-OH / 1.11 / -
C20:1 ω7c / 11.80 / -
Summed features*a
Summed Feature 2 / - / 2.13
Summed Feature 8 / 32.27 / 65.99

1, strain JL1095T; 2, Stappia stellulata IAM 12621T.

-, not detected.

a Summed Feature 2 represents C16:1 iso I/C14:0 3-OH. Summed Feature 8 represents C18:1ω7c or C18:1ω6c.

Supplementary Figure Legends:

Fig. S1 Polar lipids of strain JL1095T were reported from DSMZ Identification Services. Annotation for the components of polar lipids was as follows: PL = Phospholipid, AL = Aminolipid, PC = Phosphatidylcholin, PG = Phosphatidylglycerol, GL = Glycolipid, GL* = Glycolipid with atypical staining behavior, GL*** = Glycolipid (the Rf value and staining behavior is consistent with properties of SQDG – Sulfoquinovosyl diacylglycerol).

Fig. S2 Maximum-likelihood (ML) phylogenetic tree based on 16S rRNA gene sequences illustrating the relationship between strain JL1095T (1397bp) and phylogenetically related species. Thauera aminoaromatica S2T (AJ315677) was used to root the tree. Bootstrap values based on 1,000 replicates were showed at nodes. Values that were higher than 50% were shown. Bar, 0.02 substitutions per nucleotide position.

Figure S1

Figure S2