Supplementary Information (Supplementary Table 1-5 and Figure 1-4)

Supplementary information (Supplementary Table 1-5 and Figure 1-4)

Lineage-specific duplications of MuroideaFaim and Spag6 genes and atypical accelerated evolution of the parental Spag6 gene

Huan Qiu1,3*, Aniela Gołas2,Paweł Grzmil2, Leszek Wojnowski1

1Department of Pharmacology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany

2Department of Genetics and Evolution, Institute of Zoology, JagiellonianUniversity, PL-30-387 Krakow, Poland

3Present address: Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, New Jersey, USA

*Corresponding author:

Supplementary Table 1.Tetrapod orthologous gene groups with exclusive duplication in mouse

Family (gene name)human_gene,dog_gene,cow_gene,elephant_gene,opossum_gene,chicken_gene,lizard_gene,frog_gene,mouse_genes

Family-1 (DNAJC11)ENSG00000007923,ENSCAFG00000019632,ENSBTAG00000011217,ENSLAFG00000016487,ENSMODG00000005908,ENSGALG00000000627,ENSACAG00000012045,ENSXETG00000011143,ENSMUSG00000039768,ENSMUSG00000089825

Family-2 (DEPDC1)ENSG00000024526,ENSCAFG00000020429,ENSBTAG00000001343,ENSLAFG00000013772,ENSMODG00000000686,ENSGALG00000011275,ENSACAG00000011251,ENSXETG00000012376,ENSMUSG00000028175,ENSMUSG00000054125

Family-3 (DCUN1D1)ENSG00000043093,ENSCAFG00000011734,ENSBTAG00000012797,ENSLAFG00000016514,ENSMODG00000021289,ENSGALG00000008807,ENSACAG00000007413,ENSXETG00000016813,ENSMUSG00000027708,ENSMUSG00000074910

Family-4 (SPAG6)ENSG00000077327,ENSCAFG00000004174,ENSBTAG00000033291,ENSLAFG00000005189,ENSMODG00000009058,ENSGALG00000007892,ENSACAG00000008944,ENSXETG00000007384,ENSMUSG00000037708,ENSMUSG00000022783

Family-5 (CRLS1)ENSG00000088766,ENSCAFG00000005934,ENSBTAG00000000065,ENSLAFG00000002495,ENSMODG00000003883,ENSGALG00000009214,ENSACAG00000000839,ENSXETG00000026037,ENSMUSG00000027357,ENSMUSG00000089784

Family-6 (RPL6)ENSG00000089009,ENSCAFG00000008873,ENSBTAG00000031723,ENSLAFG00000002201,ENSMODG00000003096,ENSGALG00000004818,ENSACAG00000003060,ENSXETG00000004304,ENSMUSG00000029614,ENSMUSG00000057605

Family-7 (TRMT2A)ENSG00000099899,ENSCAFG00000014190,ENSBTAG00000019871,ENSLAFG00000022939,ENSMODG00000010321,ENSGALG00000002094,ENSACAG00000007994,ENSXETG00000006323,ENSMUSG00000089913,ENSMUSG00000022721

Family-8 (TCF20)ENSG00000100207,ENSCAFG00000000978,ENSBTAG00000000662,ENSLAFG00000011602,ENSMODG00000006993,ENSGALG00000011878,ENSACAG00000003634,ENSXETG00000013417,ENSMUSG00000041852,ENSMUSG00000071262

Family-9 (MRPL27)ENSG00000108826,ENSCAFG00000017031,ENSBTAG00000021720,ENSLAFG00000018289,ENSMODG00000020899,ENSGALG00000007682,ENSACAG00000005266,ENSXETG00000027422,ENSMUSG00000024414,ENSMUSG00000071867

Family-10 (KNG1)ENSG00000113889,ENSCAFG00000013625,ENSBTAG00000005122,ENSLAFG00000010592,ENSMODG00000015868,ENSGALG00000008678,ENSACAG00000007761,ENSXETG00000025973,ENSMUSG00000060459,ENSMUSG00000022875

Family-11 (STXBP3)ENSG00000116266,ENSCAFG00000019913,ENSBTAG00000007330,ENSLAFG00000017675,ENSMODG00000002311,ENSGALG00000002001,ENSACAG00000005770,ENSXETG00000005234,ENSMUSG00000071640,ENSMUSG00000027882

Family-12 (SRSF11)ENSG00000116754,ENSCAFG00000020423,ENSBTAG00000015474,ENSLAFG00000015882,ENSMODG00000000559,ENSGALG00000011305,ENSACAG00000013654,ENSXETG00000012371,ENSMUSG00000090195,ENSMUSG00000055436

Family-13 (DNAH7)ENSG00000118997,ENSCAFG00000010384,ENSBTAG00000016784,ENSLAFG00000011951,ENSMODG00000012112,ENSGALG00000007841,ENSACAG00000012358,ENSXETG00000022093,ENSMUSG00000067534,ENSMUSG00000067440,ENSMUSG00000067536,ENSMUSG00000041144,ENSMUSG00000079572

Family-14 (KCNIP2)ENSG00000120049,ENSCAFG00000009952,ENSBTAG00000016343,ENSLAFG00000016066,ENSMODG00000011638,ENSGALG00000007666,ENSACAG00000012032,ENSXETG00000014462,ENSMUSG00000025221,ENSMUSG00000090198

Family-15 (ACAT2)ENSG00000120437,ENSCAFG00000000720,ENSBTAG00000002827,ENSLAFG00000007904,ENSMODG00000007156,ENSGALG00000011659,ENSACAG00000004394,ENSXETG00000021174,ENSMUSG00000023832,ENSMUSG00000062480

Family-16 (SPOP)ENSG00000121067,ENSCAFG00000016942,ENSBTAG00000005824,ENSLAFG00000003311,ENSMODG00000012191,ENSGALG00000009939,ENSACAG00000004879,ENSXETG00000027799,ENSMUSG00000074424,ENSMUSG00000074432,ENSMUSG00000057522,ENSMUSG00000074426,ENSMUSG00000074428,ENSMUSG00000064290,ENSMUSG00000074425,ENSMUSG00000059676,ENSMUSG00000074427,ENSMUSG00000068879,ENSMUSG00000058005,ENSMUSG00000060256

Family-17 (ANXA11)ENSG00000122359,ENSCAFG00000015737,ENSBTAG00000020940,ENSLAFG00000023249,ENSMODG00000001145,ENSGALG00000002664,ENSACAG00000004422,ENSXETG00000018375,ENSMUSG00000021866,ENSMUSG00000079329

Family-18 (ACOT9)ENSG00000123130,ENSCAFG00000013388,ENSBTAG00000011258,ENSLAFG00000003152,ENSMODG00000007768,ENSGALG00000016351,ENSACAG00000007472,ENSXETG00000006223,ENSMUSG00000047565,ENSMUSG00000025287

Family-19 (USP45)ENSG00000123552,ENSCAFG00000003498,ENSBTAG00000018528,ENSLAFG00000008357,ENSMODG00000018098,ENSGALG00000015478,ENSACAG00000010371,ENSXETG00000006872,ENSMUSG00000040455,ENSMUSG00000090143

Family-20 (POT1)ENSG00000128513,ENSCAFG00000001754,ENSBTAG00000013180,ENSLAFG00000005796,ENSMODG00000015312,ENSGALG00000008740,ENSACAG00000001301,ENSXETG00000021222,ENSMUSG00000029676,ENSMUSG00000024174

Family-21 (PPHLN1)ENSG00000134283,ENSCAFG00000009764,ENSBTAG00000008595,ENSLAFG00000001173,ENSMODG00000008885,ENSGALG00000009547,ENSACAG00000003149,ENSXETG00000026823,ENSMUSG00000079737,ENSMUSG00000036167

Family-22 (DMTF1)ENSG00000135164,ENSCAFG00000001819,ENSBTAG00000020878,ENSLAFG00000004637,ENSMODG00000016953,ENSGALG00000006628,ENSACAG00000001448,ENSXETG00000005412,ENSMUSG00000042508,ENSMUSG00000058670

Family-23 (TES)ENSG00000135269,ENSCAFG00000003399,ENSBTAG00000021204,ENSLAFG00000017268,ENSMODG00000015840,ENSGALG00000009398,ENSACAG00000006611,ENSXETG00000011568,ENSMUSG00000068113,ENSMUSG00000029552,ENSMUSG00000090102

Family-24 (IL11RA)ENSG00000137070,ENSCAFG00000001951,ENSBTAG00000014907,ENSLAFG00000011718,ENSMODG00000007889,ENSGALG00000005848,ENSACAG00000007445,ENSXETG00000002171,ENSMUSG00000073876,ENSMUSG00000078735,ENSMUSG00000066201

Family-25 (SNRPF)ENSG00000139343,ENSCAFG00000006395,ENSBTAG00000016271,ENSLAFG00000017426,ENSMODG00000008091,ENSGALG00000011409,ENSACAG00000014073,ENSXETG00000021630,ENSMUSG00000020018,ENSMUSG00000072585

Family-26 (TDG)ENSG00000139372,ENSCAFG00000007442,ENSBTAG00000021633,ENSLAFG00000003813,ENSMODG00000002206,ENSGALG00000012722,ENSACAG00000016148,ENSXETG00000025927,ENSMUSG00000034674,ENSMUSG00000047347

Family-27 (WDR20)ENSG00000140153,ENSCAFG00000018041,ENSBTAG00000007007,ENSLAFG00000001150,ENSMODG00000013312,ENSGALG00000011365,ENSACAG00000000164,ENSXETG00000010253,ENSMUSG00000037957,ENSMUSG00000035560

Family-28 (DEGS1)ENSG00000143753,ENSCAFG00000016381,ENSBTAG00000012705,ENSLAFG00000001728,ENSMODG00000005134,ENSGALG00000009330,ENSACAG00000005458,ENSXETG00000018586,ENSMUSG00000038768,ENSMUSG00000038633

Family-29 (SLC10A4)ENSG00000145248,ENSCAFG00000001964,ENSBTAG00000004888,ENSLAFG00000003247,ENSMODG00000020633,ENSGALG00000014108,ENSACAG00000006369,ENSXETG00000009830,ENSMUSG00000060204,ENSMUSG00000029219

Family-30 (TMEM181)ENSG00000146433,ENSCAFG00000000655,ENSBTAG00000010838,ENSLAFG00000007610,ENSMODG00000007661,ENSGALG00000013737,ENSACAG00000004823,ENSXETG00000023341,ENSMUSG00000079733,ENSMUSG00000038141

Family-31 (HMBS)ENSG00000149397,ENSCAFG00000012342,ENSBTAG00000005364,ENSLAFG00000018576,ENSMODG00000013292,ENSGALG00000000291,ENSACAG00000015510,ENSXETG00000016285,ENSMUSG00000073552,ENSMUSG00000032126

Family-32 (COMMD7)ENSG00000149600,ENSCAFG00000007244,ENSBTAG00000016427,ENSLAFG00000008789,ENSMODG00000019450,ENSGALG00000006630,ENSACAG00000009735,ENSXETG00000001197,ENSMUSG00000056941,ENSMUSG00000090182

Family-33 (BRE)ENSG00000158019,ENSCAFG00000005205,ENSBTAG00000031335,ENSLAFG00000004951,ENSMODG00000015580,ENSGALG00000010039,ENSACAG00000009552,ENSXETG00000010201,ENSMUSG00000052139,ENSMUSG00000089895

Family-34 (FAIM)ENSG00000158234,ENSCAFG00000007576,ENSBTAG00000023426,ENSLAFG00000015680,ENSMODG00000018576,ENSGALG00000012082,ENSACAG00000009679,ENSXETG00000007986,ENSMUSG00000032463,ENSMUSG00000079413

Family-35 (CCDC112)ENSG00000164221,ENSCAFG00000000214,ENSBTAG00000001898,ENSLAFG00000003761,ENSMODG00000015716,ENSGALG00000023042,ENSACAG00000008012,ENSXETG00000011297,ENSMUSG00000079668,ENSMUSG00000071855

Family-36 (USP49)ENSG00000164663,ENSCAFG00000001609,ENSBTAG00000008981,ENSLAFG00000017166,ENSMODG00000012836,ENSGALG00000003461,ENSACAG00000002993,ENSXETG00000011843,ENSMUSG00000023984,ENSMUSG00000090115

Family-37 (SYTL3)ENSG00000164674,ENSCAFG00000000659,ENSBTAG00000010334,ENSLAFG00000009320,ENSMODG00000007647,ENSGALG00000013741,ENSACAG00000004780,ENSXETG00000009775,ENSMUSG00000079735,ENSMUSG00000041831,ENSMUSG00000079730

Family-38 (RBPJ)ENSG00000168214,ENSCAFG00000016387,ENSBTAG00000003602,ENSLAFG00000000410,ENSMODG00000000129,ENSGALG00000014365,ENSACAG00000014578,ENSXETG00000000935,ENSMUSG00000039191,ENSMUSG00000079575

Family-39 (NANP)ENSG00000170191,ENSCAFG00000004410,ENSBTAG00000000686,ENSLAFG00000015738,ENSMODG00000006495,ENSGALG00000010557,ENSACAG00000008452,ENSXETG00000024902,ENSMUSG00000053916,ENSMUSG00000043346

Family-40 (DENND5B)ENSG00000170456,ENSCAFG00000010680,ENSBTAG00000020315,ENSLAFG00000013024,ENSMODG00000012682,ENSGALG00000012944,ENSACAG00000012799,ENSXETG00000002680,ENSMUSG00000030313,ENSMUSG00000089845

Family-41 (FOXN2)ENSG00000170802,ENSCAFG00000002673,ENSBTAG00000015151,ENSLAFG00000012466,ENSMODG00000001169,ENSGALG00000008942,ENSACAG00000014236,ENSXETG00000022651,ENSMUSG00000090020,ENSMUSG00000034998

Family-42 (TP53RK)ENSG00000172315,ENSCAFG00000010620,ENSBTAG00000027326,ENSLAFG00000002452,ENSMODG00000016229,ENSGALG00000004469,ENSACAG00000016698,ENSXETG00000003469,ENSMUSG00000042854,ENSMUSG00000039725

Family-43 (ENDOV)ENSG00000173818,ENSCAFG00000005684,ENSBTAG00000015339,ENSLAFG00000026661,ENSMODG00000002372,ENSGALG00000006963,ENSACAG00000015645,ENSXETG00000019623,ENSMUSG00000039850,ENSMUSG00000090052

Family-44 (LARP7)ENSG00000174720,ENSCAFG00000011715,ENSBTAG00000009632,ENSLAFG00000015769,ENSMODG00000005669,ENSGALG00000012048,ENSACAG00000010662,ENSXETG00000024097,ENSMUSG00000027968,ENSMUSG00000066107

Family-45 (SPHK1)ENSG00000176170,ENSCAFG00000005073,ENSBTAG00000008507,ENSLAFG00000022816,ENSMODG00000009217,ENSGALG00000023541,ENSACAG00000008270,ENSXETG00000019540,ENSMUSG00000089750,ENSMUSG00000061878

Family-46 (MTHFR)ENSG00000177000,ENSCAFG00000016558,ENSBTAG00000020698,ENSLAFG00000011008,ENSMODG00000010976,ENSGALG00000004612,ENSACAG00000017430,ENSXETG00000013086,ENSMUSG00000044058,ENSMUSG00000029009

Family-47 (FAM100B)ENSG00000185262,ENSCAFG00000005059,ENSBTAG00000013792,ENSLAFG00000032072,ENSMODG00000008352,ENSGALG00000022447,ENSACAG00000008208,ENSXETG00000025265,ENSMUSG00000050628,ENSMUSG00000047370

Family-48 (COBRA1)ENSG00000188986,ENSCAFG00000019450,ENSBTAG00000021861,ENSLAFG00000015847,ENSMODG00000017220,ENSGALG00000008532,ENSACAG00000012467,ENSXETG00000000383,ENSMUSG00000089691,ENSMUSG00000013465

Family-49 (BLOC1S2)ENSG00000196072,ENSCAFG00000009636,ENSBTAG00000010739,ENSLAFG00000014979,ENSMODG00000010548,ENSGALG00000005794,ENSACAG00000000720,ENSXETG00000023568,ENSMUSG00000057506,ENSMUSG00000025201

Family-50 (C9orf114)ENSG00000198917,ENSCAFG00000020032,ENSBTAG00000012485,ENSLAFG00000014778,ENSMODG00000006461,ENSGALG00000004622,ENSACAG00000000010,ENSXETG00000004745,ENSMUSG00000090177,ENSMUSG00000039660

Family-51 (BRD2)ENSG00000204256,ENSCAFG00000000871,ENSBTAG00000011074,ENSLAFG00000016104,ENSMODG00000014295,ENSGALG00000000156,ENSACAG00000004190,ENSXETG00000017067,ENSMUSG00000024335,ENSMUSG00000090134

Supplementary Table 2. Genomic coordinates of Faim homologous regions (shown in Fig. 1a) in 11 sampled tetrapod genomes

* Assembly version in UCSC genome browser

Supplementary Table 3. Gene conversion detection in Faim and Spag6 coding nucleotide alignments using GENECONV

Sim / BC-KA / Fragment / Num / Num / Tot / MisM
Fragment type / Sequence pair / gscale / P value / P value / Begin / End / Len / Poly / Dif / Difs / Pen.
Faim
Global inner fragment / MouseFaim;Mouse_Gm6432 / 1 / 0.0015 / 0.0354 / 390 / 537 / 148 / 47 / 5 / 90 / 3
Global inner fragment / B-mouse_Faim;D-mouse_Gm6432 / 0 / 0.0000 / 0.0009 / 391 / 537 / 147 / 46 / 0 / 61 / 0
Global inner fragment / B-mouse_Faim;D-mouse_Gm6432 / 1 / 0.0079 / 0.1180 / 391 / 537 / 147 / 46 / 0 / 61 / 4
Global inner fragment / B-mouse_Faim;D-mouse_Gm6432 / 2 / 0.0001 / 0.0019 / 391 / 537 / 147 / 46 / 0 / 61 / 8
Global inner fragment / B-mouse_Faim;D-mouse_Gm6432 / 3 / 0.0000 / 0.0011 / 391 / 537 / 147 / 46 / 0 / 61 / 11
Global inner fragment / C-hamster_Faim;C-hamster_Gm6432 / 0 / 0.0001 / 0.0015 / 391 / 515 / 125 / 42 / 0 / 64 / 0
Global inner fragment / C-hamster_Faim;C-hamster_Gm6432 / 1 / 0.0062 / 0.0929 / 391 / 515 / 125 / 42 / 0 / 64 / 4
Global inner fragment / C-hamster_Faim;C-hamster_Gm6432 / 2 / 0.0002 / 0.0040 / 391 / 515 / 125 / 42 / 0 / 64 / 7
Global inner fragment / C-hamster_Faim;C-hamster_Gm6432 / 3 / 0.0000 / 0.0018 / 391 / 515 / 125 / 42 / 0 / 64 / 11
Global inner fragment / Rat_Faim;Rat_Gm6432 / 0 / 0.0004 / 0.0029 / 431 / 537 / 107 / 34 / 0 / 74 / 0
Global inner fragment / Rat_Faim;Rat_Gm6432 / 1 / 0.0175 / 0.1947 / 388 / 537 / 150 / 48 / 3 / 74 / 3
Global inner fragment / Rat_Faim;Rat_Gm6432 / 2 / 0.0009 / 0.0066 / 431 / 537 / 107 / 34 / 0 / 74 / 6
Global inner fragment / Rat_Faim;Rat_Gm6432 / 3 / 0.0005 / 0.0035 / 431 / 537 / 107 / 34 / 0 / 74 / 9
Spag6
Global inner fragment / Rat_Spag6;Rat_Spag6-BC061194 / 0 / 0.0120 / 0.0220 / 523 / 611 / 89 / 22 / 0 / 200 / 0
Global inner fragment / Rat_Spag6;Rat_Spag6-BC061194 / 2 / 0.0180 / 0.0366 / 523 / 611 / 89 / 22 / 0 / 200 / 5
Global inner fragment / Rat_Spag6;Rat_Spag6-BC061195 / 3 / 0.0118 / 0.0243 / 523 / 611 / 89 / 22 / 0 / 200 / 8
Global outer fragment / Rabbit / 1 / 0.0079 / 0.0153 / 1285 / 1322 / 38 / 18 / 2 / 316 / 2
Global outer fragment / Rabbit / 1 / 0.0032 / 0.0118 / 1285 / 1322 / 38 / 18 / 2 / 316 / 2
Global outer fragment / Rabbit / 2 / 0.0080 / 0.0161 / 1300 / 1322 / 23 / 11 / 0 / 316 / 4
Global outer fragment / Rabbit / 3 / 0.0079 / 0.0155 / 1300 / 1322 / 23 / 11 / 0 / 316 / 5

B-mouse: Beach mouse; D-mouse: Dear mouse; C-hamster: Chinese hamster.

Sim P value means simulated P values.

BC-KA P value means Bonferroni-corrected KA (BLAST-like) P values.

Num-Poly indicates the number of polymorphic sites in the fragment.

Num-Mat indicates the number of nonunique sites within the fragment.

Tot-Mats indicates the total number of nonunique sites for that sequence.

MisM-Pen indicates the penalty per nonunique site for this sequence.

Supplementary Table 4.EST sequences for MuroideaFaim and Spag6 genes

Gene / Species / Genbank ID / Tissue source
Faim-Gm6432 / Musmusculus / BU939370 / Testicles
Peromyscusmaniculatusbairdii(Deer mouse) / GH486028 / pooled brain and testis
GH486027 / pooled brain and testis
GH473051 / pooled brain and testis
GH527448 / pooled whole fetuses
GH527447 / pooled whole fetuses
Faim / Peromyscuspolionotussubgriseus(Beach mouse) / GH458006 / pooled brain and testis
GH458007 / pooled brain and testis
Spag6-BC061194 / Musmusculus / BU936914 / Testicles
BU937325 / Testicles
BU937878 / Testicles
BU939099 / Testicles
CA466208 / Testicles
CN838746 / Testicles
BB014594 / testis
BY353855 / testis
BY360241 / testis
CJ047125 / testis
Spag6* / Musmusculus / CJ058598 / cerebellum
CJ058871 / cerebellum
CJ062541 / cerebellum
CJ060148 / corporaquadrigemina
CJ060006 / corporaquadrigemina
CJ059306 / cortex
CJ059433 / cortex
CJ057101 / hypothalamus
CJ057292 / hypothalamus
BB666041 / oviduct
CJ066955 / oviduct
BB607863 / oviduct
BU583793 / sertolicells
CJ057563 / spinal cord
CJ057853 / spinal cord
BY730687 / stomach
BY250337 / visual cortex
BY263032 / visual cortex
CJ150567 / visual cortex
CX768136 / whole eye

* Testis-derived EST sequences for this well-studied gene are not included

Supplementary Table 5. Genomic coordinates of Spag6 homologous regions (shown in Fig. 2a) in 11 sampled tetrapod genomes

Species / Genome assembly* / DNAjc1-Bmi1-Spag6-Pip4k2a-Amrc3
Human / hg19 / chr10:22,000,000-23,482,043
Mouse / mm9 / chr2:18,107,447-19,368,062
Guinea pig / cavPor3 / scaffold_91:2,460,913-3,730,509
Rabbit / oryCun2 / chr16:38,203,389-39,495,709
Dog / canFam2 / chr2:12,960,423-14,269,804
Cow / bosTau4 / chr13:22,470,282-23,663,386
Elephant / loxAfr3 / scaffold_15:20,614,358-22,269,212
Opossum / monDom5 / chr8:250,082,701-251,650,377
Lizard / anoCar2 / chr6:15,971,928-16,595,857
Chicken / galGal3 / chr2:17,340,444-17,868,102
X. tropicalis / xenTro2 / scaffold_47:2,305,345-2,906,386
Top3b-Ppm1f
Human / hg19 / chr22:22,097,992-22,338,260
Mouse / mm9 / chr16:16,291,763-17,014,671
Guinea pig / cavPor3 / scaffold_4:19,747,663-19,936,817
Rabbit / oryCun2 / chr21:5,575,303-5,762,511
Dog / canFam2 / chr26:34,063,311-34,287,629
Cow / bosTau4 / chr17:75,309,374-75,350,979
Elephant / loxAfr3 / scaffold_70:861,298-1,104,289
Opossum / monDom5 / chr3:511,138,475-511,317,196
Lizard / anoCar2 / chrUn_GL343282:1,245,458-1,319,863
Chicken / galGal3 / chr15:453,653-538,329
X. tropicalis / xenTro2 / scaffold_12:3,396,568-3,496,876
Fgd4-Dnm1l
Human / hg19 / chr12:32,634,250-32,902,019
Mouse / mm9 / chr16:16,291,763-17,014,671
Guinea pig / cavPor3 / scaffold_54:3,428,461-3,652,874
Rabbit / oryCun2 / chr8:8,582,756-8,834,438
Dog / canFam2 / chr27:19,169,070-19,407,986
Cow / bosTau4 / chr5:83,028,368-83,272,261
Elephant / loxAfr3 / scaffold_2:21,955,335-22,234,522
Opossum / monDom5 / chr8:8,686,285-8,946,393
Lizard / anoCar2 / chr5:10,150,922-10,325,773
Chicken / galGal3 / chr1:61,039,930-61,172,434
X. tropicalis / xenTro2 / scaffold_956:201,083-245,287

* Assembly version in UCSC genome browser

Supplementary Fig. 1Gene expression levels of mouse Faim and Spag6 genes across 61 mouse organs based on the results of high-density oligonucleotide arrays downloaded from Mouse GNF1M Gene Atlas at BioGPS (

Supplementary Fig. 2 Gene expression levels of mouse Faim and Spag6 genes across 11 mouse organs based on the results of Affymetrix exon array data.‘RefSeq Gene’ track shows the exon-intron structure of the gene. Each vertical bar represents an exon. Affymetrix Exon Array 1.0: Normal Tissue’ track shows the expression level of the gene at exon resolution. Each vertical bar represents an exon in one organ. Its color indicates the expression level with green for expression level below average and red for expression level higher than average. ‘Affymetrix Exon Array 1.0: Probesets’ track shows the location and identifier of the probe set used for each exon.

Supplementary Fig. 3 RT-PCR detection of mouse Faim and Spag6 gene expression. As a control, parallel reaction was carried out using genomic DNA as starting material. Faim and Spag6 genes are labelled with white or back circles in the same way as in Figs. 1 and 2. We noted that the PCR results are not quantitative measurement of expression level, and merelyprovide the proof that mouse Faim and Spag6 genes are indeed expressed in vivo and thus indicate functions.

Total RNA was extracted from mouse tissues using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany). The DNaseI treated total RNA (2 μg) was primed with oligo-dT primer and reverse transcribed at 42OC for 2 hours using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, USA). 2 μl of cDNA was used in PCR reactions with gene specific primers. To monitor possible genomic contaminations, control reactions were carried out with genomic DNA as a template. The quality of cDNA was verified by amplifying of Atp7a transcripts. The used primers are: 5'-CGTAGCTGTTTGGGACGTAGC-3' and 5'-CTCGTATGCGAAGCCACTTATG-3' for Faim; 5'-TCAGACCCAAGACGTTGCTTTGC-3' and 5'-ACCACAACCTTTGCCAGAAA-3' for Faim-Gm6432; 5'-TTCAGTAAGGTGCTGCCACA-3' and 5'-ATCCTTACTCTCCTGTGAATCTGAGT-3' for Spag6-BC061194; 5'-TTCAGTAAGGTGCTGCCACA-3' and 5'-AGTCCAGCCACTAACCTTAAACTG-3' for Spag6; 5'-CAATAAGATCCTGGCCATTGTGGGG-3' and 5'-CGGTTACCAATGAGGACTTTG-3' for Atp7a.

Mouse 39 NAGVMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQ

N+GV+ LL+P L +VP IQQ ALAL RLANY+D+L

NSGVIFLLKPFL-SMVPIIQQMTALALRRLANYSDNLVSF------H

Guinea_pig 1 atggattcactcaagcaaccaagcgccacgatagatgtt c

acgtttttacttgttcttaatcctctggtcaagaattct a

cataatgggttctgcaataggttgttgatctttctaacc c

Mouse 88 LVYSLAEQNR---FYKKAA FVLR

++ L EQN FYK+ FVLR

NLFILTEQNLRIVFYKEKN A:V[gtc] FVLR

Guinea_pig 121 attacagcataagttagaaGTGTATTGA Intron 1 AAGCtgta

attttcaaatgtttaaaaa <2-----[180 : 3532]-2> tttg

cgttaaggtaattttaaat tgga

Mouse 109 AVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALGYIARHNT

AVGK+S +AQAIVD G LD LVICLEDF PGVKEA AWALG I++H+

AVGKYS--IAQAIVDHGPLDALVICLEDF!PGVKEAEAWALGCISKHDA

Guinea_pig 3546gggatt agcgaggcgccggcgattggt4cggaggggtgcgtatacgg

ctgaac tcacttaagctactttgtaatcgtaacacgctggtcaaac

attact atgcatttaagtaggacgacttacagtacgatattaatta

Mouse 158 LSQAVVDAGAIPLLVLCIQEPEI

LSQAVVDAGA+P VLCIQE EI

E:E[gaa] LSQAVVDAGAVP!-VLCIQESEI

Guinea_pig 3688 GGTAAGGG Intron 2 GAGAActcggggggggc1gctacgtga

<1-----[3689 :21170]-1> tcacttacgctc ttgtaacat

gaatggtaattt acttggcat

Mouse 182 ALKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMILNPDAKLKR

ALKRIAASALSDISKHSPEL QTV D GAIA LA MILN L+

ALKRIAASALSDISKHSPELTQTV!DTGAIAFLAXMILNSKGTLQY

Guinea_pig21237 gcaaaggtgcagatactcgtacag4gaggagttgtaacatagatct

ctagtcccctgatcaaccatcactacgctcttcatttacagctaa

tgagtttacatttagttagaagaacaatttcacggccctattagt

Mouse 228 VLSALSQIAKHSVDLAEMVVEAE

LS L +IAKHSVDLAEMV E +

Q:S[agt] !LSILHEIAKHSVDLAEMVFEGK

Guinea_pig21376 AGTTAAGC Intron 3 CAGGT1ctaccgagactggcggagtgga

<1-----[21377:22192]-1> tcttaatcaactatcatttaga

tatctgtaattgtgaagttaag

Mouse 252 IFPVVLTCLKDKDEYVKKNACTLIREIAKHTPE

IF VVLT LKDK+E +KKN T REIAKHTP+

IFLVVLTFLKDKNENMKKNGST!-REIAKHTPK

Guinea_pig 22262 atcggcatcagaagaaaaagaa2agagacaca

ttttttcttaaaaaataaaggc gatcaacca

ttatatctggcatacggattct agtaataca

Mouse 285 LSQLIVNAGG-VAAVIDCIGSCKGNI

QLIV+ GG DCIG CKGN

FPQLIVDTGGGMVTGTDCIGLCKGNT

Guinea_pig 22357 GTGAAAA Intron 4 CAGtcccaggagggagagagtagttagaa

<0-----[22357:23519]-0>tcatttacgggttcgcagtgtgagac

taggttcaaaggcagtcctggtagca

Mouse 310 RLPGIMMLGYVAAHSENLAMAVIISK

LPGI MLGY A SENLA+ ++ISK

VLPGITMLGYTATCSENLAIEMVISK

Guinea_pig 23598 gccgaaacgtagattgacgagagataGTGTGTT Intron 5 CAG

ttcgtcttgacccgcaatctatttca<0-----[23676:26307]-0>

gatccaggtcgcttcgcgcaagttcg

Mouse 336 GVPQLSICLSEEPEDHIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLL

GVPQLS+CLSEEPEDH+KAAAAW LG HA AV VTNTL VLL

GVPQLSVCLSEEPEDHFKAAAAWVLGWAIHF--QHAWAVTVTNTLLVLL

Guinea_pig 26308 ggccctgtctggcggctaggggtgtgtgact ccgtggagaaatcgcc

gtcatctgtcaacaaataccccgttggctat aacgctctcacttttt

tacggaccgaaaagtttgttttgcatggacc atagttccattgttgt

Mouse 85 SLYM

SLYM

Guinea_pig26449 ttta

ctat

tgtg

SupplementaryFig. 4 Translation and alignment of guinea pig Spag6 pseudogene and mouse Spag6 protein sequence. Mouse Spag6 protein (GenBank: NP_056588, 507 amino acids) was used as reference to translate and align with guinea pig Spag6 pseudogene nucleotide sequence (cavPor3, scaffold_91:3,149,430-3,175,889) using GeneWise ( ‘!’ indicates in-frame insertion (with ‘4’ beneath) or deletion (with ‘1’ or ‘2’ beneath). ‘X’ indicates in-frame stop codon.

18 Faim and Faim_Gm4632 coding-nucleotide sequences

>Faim_Gm4632_gi348041354

ATGAAAATTGCAGCTATCTGGGATGTTATTTTAAGTGATGGAGTCCACAAGATTGAATTTGAACATGGGACCACATCAGGCAAACGAGTTGTGTACGTGGATGGAAAGGAAGTGATAAGAAAAGAGTGGATGTTCCCTTTAGTGGGCAAAGAAACCTTCTGTGTTGGAGCTGCAAAAAACAAAGCTATCATACGTATACTTTCTGGCAAAGGTTGTGGTTTTAGTTACACTCTGGAAATTGATGGGAAAAGCTTCAGGAAATTCGTGGAGAACAGATCTAAAACTACCAACACCTGGGTATTGCACTTGGATGACAAAGACTTTAGAATTGTTTTGGAAAAACCCACTATGGATGTATGGTGTAATGGTAAAATAGTAGAGTCGGTGGGCGAGTTTGTAGATGACGGGACTGAGACGCACTTCAGCGTTGGGAACCACGGCTGTTACATAAAAGCCGTGAGCAGCGGGAAGAAGAGAGAAGGGATTATCCATACCCTCATTGTGGATAACAGGGAAATCCCAGAGCTCACTCAG

>Faim_Gm4632_Rat_gi109485249

ATGGTGTTTGTCTTCCGTCTTGAGTTCATCAAGGACTCACTACACATTCAAGACTGGATGTTCATCCAGCCTGGGAAAATGAAAGATGTCGTAGCCATTTGGGATGTTATTTTAAGTGATGGAGTTCACAAGATTGAATTTGAACATGGGACCACATCAGGAAAACGAGTTGTATATGTGGATGGGAAGGAAGAGGTAAGAAAAGAGTGGATGTTCCAGTTGCTGGGCAAAGAAACCTTCTGTGTTGGACCTGCAAAAACCAAAGCCACCATCCATATACTTTCTGGCAAAGGTTATGTTTTTAAATATACTCTGGAAATTGAGGGGAAAAGCTTCAAGAAATTCGTGGAGAACAGATCTAAAACCACCAACACCTGGGTATTGCACTTGGATGGCAAGGACTTTAGAATTGTTTTGGAAAAAAACACTATGGATGTATGGTGCAATGGTAAAATAATAGAGACGGCGGGCGAGTTTGTAGATGATGGGACCGAAACACACTTCAGCTTGGGGAACCACGACTGTTACATAAAGGCCGTCAGCAGCGGGAAGAGGAGAGAAGGGATCATCCACACGCTCATTGTGGATAACAGGGAAATCCCAGAGCTCACTCAGTGA

>Faim_Gm4632_DeerMouse

AGCCCTCTTTATAGCCTGGAAAAAATGACAGATCTTGTAGCTGTTTGGGATGTTGCATTAAGTGATGGAATCCACAAGATTGAATTTGAGCATGGGACCACATCAGGAAAACGAGTTGTATACGTGGATGGGAAGGAAGAGATAAGAAAAGAATGGATGTTCAAATTGGTGGGAAAAGAAACCTTCTATGTTGGAGCTGCAAAGACCAAAGCCACCATAAACATAGATGCTGTCAGTGGCTTTGCGTATGAGTACACTCTGGAAATCGATGGGAAGAGTCTCAAGAAGTACCTGGAGAACAGATCAAAGACCACTAACACCTGGGTACTGAAGCTGGATGGCGAGGACTTGAGAGTTGTTTTGGAAAAAGATACTATGGATGTTTGGTGCAATGGGCAAAAACTGGAGACAGCAGGCGAGTTTGTAGATGATGGGACTGAAACACACTTCAGTGTTGGGAGCCATGACTGCTACATAAAGGCCGTCAGCAGCGGGAAGAGGAGAGAAGGGATCATCCACACCCTCATTGTGGATAACAGGGAGATCCCAGAGagccttcagtga

>Faim_Gm4632_Chinese_hamster1_gi354480737

ATGACAGACATTGTAGCTATTTGGGATGTTATTTTAAGTGATGGAGCCCACAAGATTGAATTTGAACATGGGACCACATCAGGAAAACGAGTTGTATATGTGGATGGGAAGGAAGAGATAAGAAAGGACTGGATGTTCAATTTAGTGGGCAAAGAAACCTTCTTCGTTGGAGCAAGAAAAACCAAAGCCGCCATACGTATACTGTCTGGCAAAGGTTTTGTTTTTAAATATATCCTGGAAATTGAGGGGAAAAGCTTCAGAAAGTATGTGGAGAATAGATCAAAGACCACCAACACCTGGATACTGCGCTTGGACGGCAAGAACTTTAGAATTGTTTTGGAAAAAAACACTATGGATATATGGTGCAACGGTAAAATAATAGAGTCAGCGGGCGAGTTTGTAGATGATGGAACTGAAACACACTTCAGTGTTGGGAACCATGACTGTTACATAAAAGCTGTCAGCAGCGGGAAGAGAAGAGAAGGGATTATCCACACACTCATTGTGGATAACAGAGAAATCCCAGAGATTATCCAG

>Faim_Mouse.gi171184427

ATGACGGATCTCGTAGCTGTTTGGGACGTAGCATTAAGTGACGGAGTCCACAAGATTGAATTTGAACATGGGACCACATCAGGCAAGCGGGTTGTGTACGTGGATGGGAAGGAAGAGATAAGAAGAGAGTGGATGTTCAAGTTGGTGGGCAAAGAAACGTTCTTTGTCGGAGCTGCAAAAACCAAAGCCACCATCAATATAGATGCCATAAGTGGCTTCGCATACGAGTACACGCTGGAAATTGATGGGAAGAGCCTCAAGAAGTACATGGAGAACAGGTCAAAGACCACCAGCACCTGGGTGCTGCGCCTGGATGGCGAGGACCTGAGAGTTGTTTTGGAAAAAGACACTATGGACGTATGGTGCAATGGTCAGAAAATGGAGACAGCGGGCGAGTTTGTAGATGATGGGACTGAGACGCACTTCAGCGTTGGGAACCACGGCTGTTACATAAAAGCTGTGAGCAGCGGAAAGAGGAAAGAAGGGATTATCCATACCCTCATTGTGGATAACAGGGAAATCCCAGAGCTCACTCAGTGA

>Faim_Rat_gi18266697

ATGACAGATCTCGTAGCTGTTTGGGACGTAGCATTAAGTGACGGAGTGCACAAGATTGAATTTGAGCATGGGACCACGTCAGGCAAACGAGTTGTGTACGTGGATGGGAAGGAAGAGATAAGAAGAGAGTGGATGTTCAAATTGGTGGGCAAAGAAACGTTCTTTGTCGGAGCTGCAAAGACCAAAGCCACCATAAATATAGACGCCATAAGTGGCTTCGCCTATGAGTACACACTGGAGATCGACGGGAAGAGCCTCAAGAAGTACATGGAGAACAGATCTAAGACCACCAACACCTGGGTATTGCACTTGGACGGCGAGGAACTGAGAGTTGTTCTGGAAAAAGACACTATGGATGTATGGTGCAATGGTCAGAGGATGGAGACAGCGGGCGAGTTTGTAGATGACGGGACTGAAACACACTTCAGCGTGGGGAACCACGACTGTTACATAAAGGCCGTCAGCAGCGGGAAGAGGAGAGAAGGGATCATCCACACGCTCATTGTGGATAACAGGGAAATCCCAGAGCTCACTCAGTGA

>Faim_BeachMouse.Contig1

CACAGCCTAAGGAAACTGACAGATGTTGTAGCTGTTTGGGATGTTATATTAAGTGATGGAATCCACAAGATTGAATTTGAACATGGGACCACATCAGGAAAACGAGTCGTATATGTGGATGGGAAGGAAGAGATAAGAAAAGAGTGGATGTTCAATTTGGTGGGCAAAGAAACCTTCTATGTTGGAGCTGCAAAAACCAAAGCCACCATACATATACTTTCTGACAAAGGTTATGTTTTTAAATATATCCTGGAAATTGAGAGGAAAAGCTTCGTAAAGTATGTAGAGAACAGATCAAAAACCACCAACACCTGGGTACTGCACTTGGATGGCGAGAACTTTAGAATTGTTTTGGAAAAAAACAGTATGGATGTGTGGTGCAACGGTGAAATAATAGAGTCAGCGGGCGAGTTTGTAGATGATGGGACTGAAACACACTTCAGTGTTGGGAGCCATGACTGCTACATAAAGGCCGTCAGCAGCGGGAAGAGGAGAGAAGGGATCATCCACACCCTCATTGTGGATAACAGGGAGATCCCAGAGagccttcagtga

>Faim_Chinese_hamster2_gi354480710

ATGACAGATCTTGTAGCTGTTTGGGACGTTGCATTAAGTGATGGAGTCCACAAGATTGAATTTGAGCATGGGACCACATCAGGCAAACGAGTTGTGTACGTGGATGGGAAGGAAGAGATAAGAAAAGAATGGATGTTCAAATTGGTGGGCAAAGAAACCTTCTGTGTTGGAGCTGCGAAAACCAAAGCCACCATAAATATAGATGCTGTCAGTGGTTTTGCTTATGAGTATACCCTGGAAATCGATGGGAAAAGCCTCAAGAAGTACATGGAGAACAGATCAAAGACCACCAACACCTGGGTACTGCACTTGGATGGCCAGGACTTAAGAGTTGTTTTGGAAAAAGATACTATGGATGTATGGTGCAATGGTCAAAAAATGGAGACAGCAGGCGAGTTTGTAGATGATGGAACTGAAACACACTTCAGTGTTGGGAACCATGACTGTTACATAAAAGCTGTCAGCAGCGGGAAGAGAAGAGAAGGGATTATCCACACACTCATTGTGGATAACAGGGAGATCCCAGAGCTCCCTCAG

>Faim_GuineaPig_cavPor3

AGTCCTCCTTACAGCCTGGAAAAAATGACTGATTTCGTAGCTGTTTGGGATGTTGCTTTGAGTGATGGAGTCCACAAGATTGATTTAGAACATGGGACCACATCGGGCAAACGAGTAGTATATGTAGATGGAAAGGAAGAGATAAGAAAAGAATGGATGTTCAAACTAGTGGGCAAAGAAACCTTCTATGTTGGAGCTGCAAAGACAAAAGCAACCATAAATATAGATGCTGTCAGTGGTTTTGCTTATGAATATACTCTGGAAATTAATGGGAAAAGCCTCAAGAAGTATATGGAAAATAGATCAAAAACTACCAATACGTGGGTAATGAACTTGGATGGTGAGGACTTTAGAGTTGTTCTGGAAAAAGACACCATGGACGTATGGTGCAATGGTAAAAAGATGGAGACAGCGGGTGAGTTTGTAGATGATGGGACTGAAACACACTTCAGTATTGGGAACCATGACTGTTACATAAAGGCTGTCAGTAGCGGGAAGCGGAAAGAAGGGATTATTCATACTCTCATTGTGGATAACAGAGAAATCCCAGAGATTcttaaatga

Faim_Thirteen-lined_ground_squirrel

ATGACAGATCTCGTAGCAGTCTGGGAGGTTGCTTTAAGTGATGGAGTCCACAAGATTGAATTTGAACATGGAACTACATCTGGCAAACGGGTGGTGTATGTAGATGGGAAGGAAGAGATAAGAAAAGAATGGATGTTCAAATTAGTGGGCAAAGAAACCTTCTGTGTTGGAGCTGCCAAGACAAAAGCAACCATAAATATAGATGCTATCAGTGGTTTTGCTTATGAATATACTCTGGAAATTAATGGGAAAAGTCTCAAGAAATACATGGAGAACAGATCAAAAACTACCAATACATGGGTATTACGCTTGGATGGTGAGGACTTGAGAGTTGTTTTAGAAAAAGACACCATGGATGTGTGGTGCAATGGTAAAAAGATGGAGACGGCGGGTGAGTTTGTAGATGACGGGACTGAAACACACTTCAGCGTTGGGAGCCACAACTGCTACATAAAGGCCGTCAGTAGCGGGAAACGGAAAGAAGGGATTATTCACACACTCATTGTGGATAACAGAGAAATCCCAGAGATGCTCGAA

>Faim_Rabbit_gi291399858

ATGACAGATCTTGTAGCTGTTTGGGATGTTGCTTTAAGTGACGGAATCCACAAGATTGAATTTGAACATGGCACCACATCAGGCAAACGAGTAGTGTATGTAGATGGGAAGGAAGAGATAAGAAAAGAATGGATGTTCAAATTAGTGGGCAAAGAAACCTTCTGTGTTGGAGCTGCAAAGACGAAAGCAACCATAAATATAGATGCTGTCAGTGGTTTTGCTTATGAGTATACTCTGGAGATTAATGGGAAAAGTCTCAAGAAGTATATGGAGAACAGATCAAAAACCACCCACACGTGGGTGCTACACTTGGATGGTGAGGACTGTAGAGTTGTTTTGGAAAAAGACACTATGGATGTGTGGTGCAATGGTAAAAAAATGGAGACAGCAGGAGAATTTGTGGACGATGGGACTGAGACACACTTCAGCTTCGGCAATCATGACTGTTACATAAAGGCGGTGAGCAGTGGGAAGCGGAAGGAAGGGATTATTCACACGCTCATCGTGGACAACAGAGAAATCCCCGAG

>Faim_Human_gi74271916

ATGGCATCTGGAGATGACAGTCCTATCTTTGAAGATGATGAAAGCCCTCCTTACAGCCTAGAAAAAATGACAGATCTCGTAGCTGTTTGGGATGTTGCTTTAAGTGACGGAGTCCACAAGATCGAATTTGAACATGGGACTACATCAGGCAAACGAGTAGTATATGTAGATGGAAAGGAAGAGATAAGAAAAGAGTGGATGTTCAAATTAGTGGGCAAAGAAACATTCTATGTTGGAGCTGCAAAGACAAAAGCGACCATAAATATAGACGCTATCAGTGGTTTTGCTTATGAATATACTCTGGAAATTAATGGGAAAAGTCTCAAGAAGTATATGGAGGACAGATCAAAAACCACCAATACTTGGGTATTACACATGGATGGTGAGAACTTTAGAATTGTTTTGGAAAAAGATGCTATGGACGTATGGTGCAATGGTAAAAAATTGGAGACAGCGGGTGAGTTTGTAGATGATGGGACTGAAACTCACTTCAGTATCGGGAACCATGACTGTTACATAAAGGCTGTCAGTAGTGGGAAGCGGAAAGAAGGGATTATTCATACTCTCATTGTGGATAATAGAGAAATCCCAGAGATTGCAAGTTAA

>Faim_Dog_gi345789255

ATGGCATCTGGAGATGACAGTCCTATCTTTGAAGATGATGAAAGCCCTCCTTATAGCCTGGAAAAAATGACAGATCTCGTAGCCGTTTGGGATGTTGCTTTAAGTGATGGAGTCCATAAGATTGAGTTTGAACATGGCACCACATCAGGCAAACGAGTGGTATATGTAGATGGGAAGGAAGAGATAAGAAAAGAATGGATGTTCAAACTAGTGGGCAAAGAAACTTTCTGCGTTGGAGCTGCAAAGACAAAAGCAACCATAAATATAGATGCTGTCAGTGGCTTTGCGTATGAATATACTCTGGAAATTAATGGCAAAAGTCTCAAGAAGTATATGGAGAACAGGTCGAAGACTACCAATACCTGGGTATTACATTTGGATGGTGAGGACTTTAGAGTTGTGTTGGAAAAGGACACTATGGATGTATGGTGCAATGGTAAAAGAATGGAGACAGCAGGTGAGTTTGTAGAGGACGGGACTGAAACTCACTTCAGTGTTGGGAACCATGACTGTTACATAAAGGCTGTCAGTAGTGGGAAGCGGAAAGAAGGGATTATCCATAGTCTCATTGTGGATAATAGAGAAATTCCAGAGATCCTTGAATGA

>Faim_Cow_ENSBTAT00000031927

ATGGCATCTGGAGATGACAGTCCTATCTTTGAAGATGACGAAAGCCCTCCTTACAGCCTGGAAAAAATGACAGATCTTGTAGCTGTTTGGGAAGTTGCTTTAAGTGATGGAGTCCATAAAATTGAATTTGAACATGGGACCACATCAGGCAAACGAGTGGTGTATGTAGATGGGAAGGAAGTGATAAGAAAAGAATGGATGTTCAAATTGGTGGGCAAAGAAACCTTCTGCGTTGGAGCTGCAAAGACAAAAGCAACCATAAATATCGATGCTGTCAGTGGTTTTGCTTATGAATATACTCTGGAAATTAATGGGAAAAGTCTCAAGAAGTATATGGAGAACAGATCAAAAACCACCAATACTTGGGTATTACATTTGGATAGTGAGGACTTTAGAGTTGTGTTAGAGAAAGACACTCTGGATGTGTGGTGCAATGGTAAAAAAATGGAGACAGCGGGTGAGTTTGTAGATGATGGGACTGAAACTCACTTCAATATTGGGAATCATGACTGTTACATAAAGGCTGTCAGTAGCGGCAAGCGGAAAGAAGGGATTATTCATAGTCTCATTGTGGATAACAGAGAAATCCCAGAGATTGTTGAATGA

>Faim_Elephant_gi344296625.manuallycurated

ATGACAGATCTCGTAGCCGTTTGGGATGTTGCTTTAAGTGATGGAGTCCACAAGATTGAATTTGAACATGGGACCACATCAGGCAAACGTGTGGTATATGTAGATGGAAAGGAAGAGATAAGAAAAGAGTGGATGTTCAAATTAGTGGGCAAAGAAACCTTCTGTGTTGGAGCTGCAAAGACGAAGGCAACCATAAACATAGATGCTGTCAGTGGTTTTGCTTATGAATACACCCTGGAAATTAATGGGAAAAGCCTCAAGAAGTATATGGAGAATAGATCAAAAACCACCAATACCTGGGTATTACACTTGGATGGTGAGGACTTTAGAGTTGTGTTGGCTATGGATGTTTGGTGCAATGGTAAAAAAATGGAGACTGCAGGTGAGTTTGTAGATGACGGAACTGAAACTCACTTCAGTATTGGGAACCATGACTGTTACATAAAGGCTGTCAGTAGTGGCAAGCGAAAAGAAGGGATTATTCATACTCTCATTGTGGATAATAGAGAAATCCCAGAAATTCTTGAA

>Faim_Opossum_ENSMODT00000023586

ATGGCATCCCAAGATGAGATTGCTCTCTTTGAAGATGAAATAAGATCTTGTTCCAGCCAGTTTGAAAAAATGACAGATCTTGTAGCTGTTTGGGATGTTGCCTTAAGTGATGGTGTTCACAAGATTGAATTTGAACATGGAACTACTTCAGGAAAAAGGGTTGTGTATGTAGATGGAAAGGAAGAGGTAAGAAGAGAATGGATGTTCAAGCTGGTTGGCAAAGAAACATTTAGTGTTGGAGCTGCAAAAACAAAAGCTACTATAAACATAGATGCAGTCAGTGGTTTTGCTTATGAATATACTCTGGAAATTGATGGGAAAAGTCTCAAGAAATATATGGAAAATAGATCAAAAACAACAAATACTTGGATTTTACATTTGGATGGTATGGATTTTAGAATTGTTTTGGAGAAAGATACTATGGATGTCTGGTGCAATGGTAAAAAAATGGAGACAGCCGGTGAGTTTGTAGATGACGGAACTGAAACTCACTTCAGTATTGGTAATCATGAATGTTATATAAAAGCTGTCAGTAGTGGAAAGCGAAGAGAAGGGATTATTCATACTCTAATAGTGGATGATAAAGAAATTGCAGAGACTTTGGAGTAA

>Faim_Chicken_chr7:27773376-27777290

ATGGCGTCCCGTGAGGAGGTTACCCTTAGTGAAGAAGAAGTAAGGTCTCGGTACAGCCACTTAGAGAAGATGACAGATTTGGTGGCTGTTTGGGAAGTAGCTTTAAGCGATGGTGTTCATAAAATTGAGTTTGAGCACGGGACCACTTCAGGAAAACGTGTTGTCTATGTTGACGGAAAGGAAATAATACGAAAAGAATGGATGTTTAAATTAGTGGGTAAAGAAACATTCACTGTTGGAGTATCCAAAACAAGAGCCACTATTAACATTGATGCAGTCAGTGGCTTTGCATATGAGTATACTTTGGAGATCAATGGAAAAAGCCTCAAGAAATATATGGAGAACAGGTCAAAAACAACCAATACTTGGGTACTGAGCTTGGGTGGTACAGACTATAGAGTTGTTCTGGAAAAGGACACTATGGATGTGTGGTGCAACGGTGAAAAAATGGAGACAGCGGTACGTGATCACTTCCACAGGTTTTTCATAGACTATGGCTCTAGTATAACCAAAGGTGAATTTGTAGAAGATGGGACTGAAACTCACTTCAGTGTTGGTGATCACAGCTGTTACATTAAGGCTGTCAGTAGTGGAAAACGAAAGGAAGGAATTATTCACACTCTTATTGTGGATGACAGAGAAATTCCAGAGGCTGTGGAGTAG

Faim_Lizard

ATGGCATCCCGTGATGAGAATACTAACTGTGAAGATGATGTAAGCTCTGACTACAGTTACCTGGACAGAATGACAGATCTGGTAGCTATCTGGGAAGTAGCTTTAAGTGATGGGGTTCACAAAATTGAATTTGAACACGGAACTACCTCAGGAAAACGTGTCATATATGTGGATGGAAAGGGACTCAGGACGGTCGACAACAATGGCAACAATTTGATGCCCATACAAATCACTATTAAAATACATTTGTACACTTTTTCAGCATCCAAAACAAAAGCTACCATCAACATTGATGCCGTCAGTGGCTTTGCATATGAATATACATTGGAAATTGATGGGAAAAGCCTAAAAAAGTACATGGAGAACAGGTCAAAGACAACAAACACTTGGTTATTACACCTGGATGGCACAGACTTTAGAGTTGTGTTAGAGAAAGACACAATGGATGTTTGGTGCAATGGTAAAAAAATGGAAACAGCGGTATGTATAATTTTTAATTATTGTTTTATTTTTTGCGGTGAATTTGTCGAAGATGGAACGGAAACACATTTCAGTATTGGAAAGCACAGCTGTTGCATTAAAGCTATCAGTAGCGGGAAGAGAAGGGAAGGAATTCTTCATATGCTGATTGTTGATGACGGAGAAATCCCAGAAGCTTTGGAA

14 Spag6 and Spag6-BC061194 coding-nucleotide sequences

>Spag6_Mouse_gi226693359

ATGAGCCAGCGGCAGGTGCTGCAAGTGTTCGAGCAGTACCAGAAAGCCCGGACGCAATTCGTGCAGATGGTGGCAGAGCTGGCGACAAGACCCCAAAACATCGAAACGCTGCAGAATGCAGGTGTAATGTCTTTGCTAAGGCCGCTTCTTCTGGATGTGGTCCCAACAATTCAACAGACTGCAGCTTTGGCTCTGGGGAGACTGGCCAATTACAATGATGACTTAGCAGAAGCAGTCGTGAAAGGTGACATTCTTCCACAGCTTGTTTATTCATTGGCAGAACAAAATCGTTTTTACAAGAAGGCAGCTGCCTTTGTGTTGAGAGCAGTTGGGAAACACTCTCCTCAGCTGGCCCAGGCCATAGTTGACTGCGGAGCTCTGGATACACTGGTGATCTGCTTGGAGGATTTTGACCCTGGGGTCAAGGAGGCTGCAGCCTGGGCACTCGGATACATTGCAAGACACAACACAGAATTATCTCAAGCCGTGGTGGATGCAGGAGCAATTCCTCTTTTAGTCCTTTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATCGCTGCCTCGGCCCTCAGTGATATCTCAAAGCACTCCCCAGAGTTAGCACAGACGGTGGTGGACGCAGGAGCTATTGCTCACTTAGCCCAGATGATCCTCAACCCTGATGCAAAACTGAAGCGCCAGGTCCTCTCAGCTCTCAGTCAGATTGCAAAGCATTCTGTGGACTTGGCAGAGATGGTTGTTGAAGCAGAGATTTTCCCTGTTGTACTAACCTGCCTGAAGGACAAAGATGAATATGTGAAGAAAAATGCTTGCACCTTAATCAGAGAGATTGCAAAACACACGCCTGAGCTCTCGCAGCTGATTGTTAATGCAGGAGGCGTGGCTGCCGTGATCGATTGCATTGGGTCCTGCAAAGGGAACATACGGCTGCCTGGCATCATGATGCTGGGTTACGTGGCTGCTCATTCTGAGAACCTGGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAATCTGCCTGTCAGAAGAACCAGAAGATCATATTAAGGCTGCTGCTGCCTGGGCCTTGGGGCAGCTGGGAAGACACACTCCAGAGCATGCCAGGGCTGTGGCTGTCACAAACACGCTGCCAGTCCTGCTGTCTTTGTACATGTCACCAGAGAGCTCTGAGGACCTACAGCTCAAAAGTAAAAAAGCCATAAAGAATATCCTTCAGAAATGCACCTACCTCCCAGCCCTTGAGCCGTTCCTGTACGACGCACCTCCCAATATCCTGAAACATGTTGTTGGGCAGTTCAGTAAGGTGCTGCCACATGACAGCAAAGCCCGGCGGCTTTTCGTGACTAGTGGTGGACTTAAAAAGGTTCAGGAGATAAAGGCAGAACCAGGCTCTCTCCTGCAGGAATACATCAACAGCATCAACAATTGTTACCCAGAGGAAATAGTAAGGTATTATTCACCTGGATACTCCGATACACTTCTGCAGAGGGTGGACAGCTACCAACCACTG

>Spag6_Rat_gi77993362

ATGAGCCAGCGGCAGGTGCTTCAAGTGTTCGAGCAGTACCAGAAAGCCCGGACGCAATTCGTGCAGATGGTGGCAGAGCTGGCGACCAGACCCCAAAACATAGAAACGCTACAGAATGCAGGTGTAATGTCTTTGCTAAGGCCGCTTCTTCTGGATGTGGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTGGGGAGACTGGCGAATTACAATGATGACCTGGCAGAAGCAGTTGTGAAGGGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAGAACAAAATCGTTTCTACAAAAAGGCAGCTGCCTTTGTGCTGAGAGCAGTTGGTAAACACTCTCCTCAGCTGGCCCAGGCCATAGTTGACTGTGGAGCTCTGGATACACTGGTGATATGCTTGGAGGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCCTGGGCACTTGGATATATTGCAAGACACAATACAGAATTATCTCAAGCTGTGGTGGATGCAGGAGCAATTCCTCTTTTGGTTCTGTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCCTCGGCCCTCAGTGATATCTCAAAGCACTCCCCAGAGTTAGCGCAGACAGTGGTGGATGCCGGAGCCATTGCTCACCTAGCCCAGATGATCCTCAACCCTGATGCCAAACTGAAGCGCCAGGTACTCTCAGCTCTCAGTCAGATTGCAAAACACTCTGTGGACCTGGCAGAGATGGTCGTTGAAGCAGAAATTTTCCCTGTTGTACTAACCTGCCTGAAGGACAAAGATGAATATGTGAAGAAAAATGCTTGCACCTTAATTAGAGAAATTGCAAAACATACGCCTGAGCTCTCCCAGCTGATTGTTAATGCCGGAGGTGTGGCTGCTGTGATTGACTGCATCGGATCCAGCAAAGGGAACATACGGCTCCCTGGCATCATGATGCTGGGCTATGTGGCTGCTCATTCTGAGAACCTGGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAATCTGCCTGTCAGAGGAACCAGAAGATCATATTAAGGCTGCTGCTGCCTGGGCCCTGGGGCAGCTGGGAAGACACACTCCAGAGCATGCCAGGGCTGTAGCTGTCACAAACACTCTGCCAGTTCTCCTGTCTTTGTACATGTCAGCAGAGAGCTCTGAGGACCTGCAGCTGAAGAGTAAAAAAGCCATAAAGAACATCCTTCAAAAATGTACCTACCTACCAGCCCTCGAGCCATTCTTGTACGATGCACCTCCCAATATCCTGAAACATGTTGTTGGGCAGTTCAGTAAGGTGCTGCCACACGACAGCAAAGCCCGACGGCTTTTTGTGACAAGTGGTGGACTTAAAAAGGTCCAGGAGATAAAAGCAGAGCCAGGCTCTCTCCTGCAGGAATACATCAACAGCATCAACAATTGCTACCCAGAAGAAATAGTAAGGTATTATTCGCCTGGATACTCTGAGACACTTCTGCAGAGGGTGGACAGCTACCAGCCACAG

>Spag6_Mouse_gi110625691

ATGAGCCAGCGGCAGGTGCTGCAAGTGTTCGAGCTGTACCAGAACGCCCGGACCCGGTTTGTGCAGATGGTGGCGGAGCAGGCGACCAAACCCCAGAACATCGAGACACTGCAGAATGCGGGTATAATGTCTTTGCTGAGGCCTCTTCTTCTGGACGTGGTCCCAACCATTCAGCAGACTGCTGCCTTGGCTCTGGGGAGGCTGGCCAACTACAACGATGACTTAGCAGAAGCAGTCGTGAAGGGTGACATTCTTCCACAGCTTGTGTATTCATTGGCAGAGCAGAATTGTGTCTACAAGAAGGCAGCTGCGTTTGTGCTGCGAGCGGTTGGTAAACACTCTCCTCAGCTGGCGCAGGCCACAGTTGACTGTGGTGCCCTGGACTCACTGGTGATCTGCTTGGAAGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCCTGGGCACTTGCATATATTGCAAGGCATAATGCAGAACTGTCGCAAGCTGTGGTGGATGCGGGAGCTGTTCCTCTTTTAGTCCTCTGTATCCAGGAGCCCGAGACTGCTTTGAAAAGGATCGCTGCCTCGGCCCTCAGTGATATTTCAAAGCACTCCCCAGAGTTAGCACAAACGGTGGTGGATGTAGGAGCTATTGCTCACTTAGCCCAGATGATCCTCAACCCCGATGAGAAACTGAAGCAACAGGTCCTCTCAGCTCTGAGTCACATCGCGAAGCACTCGGTGGACCTGGCAGAGATGGTTGTGGAGGCAGAGATTTTCCCAGTTGTTCTCACCTGTCTGAAGGACAAAGATGACTTCGTGAAGAAGAATGCTTGTACTCTGATTAGAGAGATCGCAAAACATACACCAGAGCTCTCGCAGCTGATTGTTAATGCCGGAGGTGTGGCTGCCGTGATCGACTGCATCGGGTCCTGCAAAGGGAACATACGGCTGCCTGGCATCATGATGCTGGGTTACATGGCTGCTCATTCTGAGAACCTGGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAGACTGCCTGTCAGAAGAACCGGAAGATCATATTAAGGCTGCGGCTGCCTGGGCTCTAGGGCAGGTTGGGAGGCACACTCCAGAACATGCTCGGGCTGTCGCCATCACAAACACGTTGCCCGTGCTGCTGGCTTTGTACATGTCCCCAGAGAGCTCTGAGGACCTGCAGGTGAAAAGTAAAAAGGCCATAAAGAATATCATCCAAAAATGCACCTACCTCCCGGCGCTTGAGCCGTTTCTCTATGATGCGCCTCCCAATATCCTGAAGTATGTCGCTGGACAGTTCAGTAAGGTGCTGCCACATGACAGCAAAGCTCGTCGGCTTTTTGTAACAAGTGGTGGACTTAAAAAGATCCAAGAGATCAAAGCAGAACCTGGTTCCATCCTCCAAGAGTATATCAACAACATCAACAGCTGCTACCCAGAGGAAATAGTAAGGTACTATTCTCCTGGATACTCAGACATACTTCTGCAGAGGATCGACAGCTATCAGCCTCTTattaac

>Spag6_Rat_gi157822990

ATGAGCCAGCGGCAGGTGCTGCAAGTGTTCGAGCTGTACCAGAACGCCCGGACCCGGTTCGTGCAGATGGTGGCGGAGCAGGCGACCAGACCTCAGAACATTGAGACACTGCAGAATGCGGGTATAATGTCTTTGCTGAGACCTCTTCTTCTGGATGTGGTGCCAACCATTCAACAGACTGCTGCCTTGGCTCTGGGGAGGCTGGCCAACTATAACGATGACTTAGCAGAAGCAGTTGTGAAGGGTGACATTCTTCCACAGCTTGTCTACTCACTGGCAGAGCAGAATTGCGTCTACAAGAAAGCGGCTGCGTTTGTGCTGCGAGCGGTTGGCAAACATTCTCCTCAGCTGGCGCAGGCTACAGTTGACTGTGGGGCCCTGGACTCACTGGTGATATGCTTGGAAGATTTTGACCCAGGAGTCAAGGAAGCTGCAGCCTGGGCACTTGCATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGACGCGGGAGCTGTTCCTCTTTTAGTCCTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCCTCGGCCCTCAGTGATATCTCAAAGCACTCCCCAGAGTTAGCGCAGACTGTGGTGGATGTAGGAGCCATTGCTCATTTAGCCCAGATGATCCTCAACCCTGATGAAAAACTGAAGCAACAGGTCCTGTCAGCTCTGAGTCACATCGCGAAGCACTCTGTGGACCTGGCGGAGATGGTTGTGGAGGCAGAGATCTTCCCTGTTGTTCTTACCTGTCTGAAGGACAAAGACGACTTTGTGAAGAAGAACGCTTGCACTCTGATCAGAGAGATCGCAAAGCACACACCGGAGCTCTCCCAGCTGATCGTTAACGCAGGAGGCGTGGCCGCCGTGATTGACTGCATTGGGTCCTGCAAAGGGAACATCCGTTTGCCTGGCATCATGATGCTGGGTTATGTGGCTGCTCATTCTGAGAACCTGGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAGACTGCCTGTCAGAAGAACCAGAAGACCATATTAAAGCCGCGGCTGCCTGGGCTCTGGGGCAGGTCGGGAGACACACTCCGGAACACGCACGCGCAGTCGCTATCACGAACACGCTGCCAGTGCTGCTGGCTTTGTACATGTCCTCAGAGAGCTCTGAGGACCTGCAGGTGAAGAGTAAAAAGGCCATAAAGAATATCATCCAAAAATGCACCTACCTCCCAGCTCTTGAGCCGTTTCTCTATGATGCGCCTCCCAATATCCTGAAGTATGTGGCTGGACAGTTCAGTAAGGTGCTCCCACATGACAGCAAAGCTCGAAGGCTTTTTGTAACAAGTGGCGGACTTAAAAAGATCCAGGAGATAAAAGCAGAACCGGGTTCTATCCTCCAAGAGTACATCAACAACATCAACAGCTGCTACCCAGAGGAGATAGTGAGGTATTATTCTCCTGGATACTCAGACATCCTTCTGCAGAGGGTGGACAGCTATCAGCCCCTCgctaac

>Spag6_GuineaPig_XM_003462725

ATGAGCCAGAGGCAAGTGCTGCAAGGACTTGAGGAGTACCAGAAAGCCCGGCTCCAGTTCGTGCAGACGGTGGCAGAACAAGCGACCAGGCCCCAGAACATCGAGACGCTGCAAAACGCGGGTGTAATATCTTTGCTGAGGCCTCTTCTTCTCGATGTGGTCCCAACAATTCAGCAGACAGCGGCTTTGGCTCTTGGGAGACTGGCCAATTACAGCGATGACTTAGCAGACGCAGTTGTAAAGGGGGACATCCTTCCACAACTTGTTTATTCATTGGCAGAGCAGAATCGTTTCTACAAGAAAGCCGCTGCATTTGTTTTGAGAGCAGTTGGGAAGCATTCTCCCCAGAGAGCGCAGGCCATAGTTGACTGTGGAGCACTGGATGCACTGGTGATATGCTTGGAAGACTTTGACCCTGGAGTCAAGGAGGCTGCAGCCTGGGCACTTGGATGTGTTTCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGATGCGGGAGCTGTTCCTCTCTTAGTGCTCTGTATCCAGGAGCCAGAGATTGCTCTGAAAAGGATTGCGGCCTCAGCGCTCAGTGATATTTCAAAGCATTCTCCAGAGTTAGCACAGACAGTGGTGGACGCAGGAGCTATCGCTCACTTAGCCCAGATGATCCTCAATCCTGATGCTAAATTGAAGCAACAAGTCCTTTCAGCTCTCAGTCAGATTGCAAAACATTCTGTGGATCTGGCAGAAATGGTTGTTGAAGCAGAGATTTTTCCCGTTGTACTTACCTGTCTGAAGGACAAAGATGAATATGTGAAGAAAAACGCTTGCACTTTAATTAGAGAGATTGCAAAACATACACCTGAGCTTTCACAGCTGATCGTTAACACAGGAGGGGTGGCCGCAGTGACCGACTGCATTGGGACCTGCAAAGGGAACACACGGTTGCCTGGCATCATGATGCTAGGTTATGTGGCTGCTCACTCCGAGAACCTAGCCATGGCAGTGATCATTTCTAAGGGTGTACCCCAGCTGTCCATCTGCCTGTCAGAAGAACCAGAGGATCATATTAAGGCTGCAGCTGCCTGGGCCTTAGGACAGATTGGGCGACACACTTCCGAACATGCTCGGGCAGTCGCCGTCACAAATACTTTGCCCGTTCTGCTTTCTCTGTATATGTCCACAGAACGTTCTGAGGATCTGCAAATGAAATGCAAAAAAGCCATAAAAAGCATCCTACAGAAATGTACCTACCTACCAGCGCTGGAGCCATTTCTGTATGACGCGCCTCCAAATATTCTGAAGCATGTGCTTGGGCAGTTCAGCAAGGTGCTGCCACATGATAGCGAAGCACGCCGACTTTTTGTAACAAGTGGTGGACTTAGAAAAGTTCAAGAGATACAAGCAGAACCTGGTTCTCTCCTGCAAGACTATATCAACAGTATTAACAACTGTTACCCAGAGGAAGTGGTAAGGTACTATTCCCCTGGATATTCAGACACACTTCTTCAAAGAGTGGACAACTATCAGCCAGTTattgat

>Spag6_Rabbit

GTGTTCGAGCAGTACCAGAAAGCCCGCGTCCAGTTCGTGCAGATGGTAGCGGAGCTGGCGAACAAACCGCAGAACATCGAGATCCTGCAGAACGCGGGTGTCATATCTTTACTGCGGCCCCTTCTTCTGGATGTCGTCCCTTCAGTCCAACAGACCGCGGCGCTGGCTCTTGGGAGACTGGCCAATTACAATGAAGACCTAGCAGAAGCCATTGTGAAGGACAACATCCTTCCACAGCTTGTTTATTCGATGACAGAGCAGAATCGTTTCTACAAGAAAGCAGCTGCGTTTGTGTTCCGAGCAGTTAGCAAACACTCGCCCCAGCTCGCTCAAGCGGTGGTCGACTGCGGCGCGCTGGGCATTCTGGTGATATGCCTGGAAGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCTTGGGCCCTTGGATATATCTCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGATGAGGGAGCTGTTCCTCTTTTAGTCCTCTGTATCCAGGAGCCAGAGATTGCTCTGAAAAGGGTTGCGGCCTCGGTCCTCAGCGATATCGCCAAGCATTGTCCGGAATTAGCACACGCCGTGGTGGATGCAGGAGCCATCCCTCACCTCGCTCAGATGATCGTCAGTCCCAATGCGAAACTGAAGTGCCAGGTCCTTTCTGCGCTCAGCCAGATTGCCAAACACTGTGTGGACCTCGCAGAGATGGTCGTGGAAGCGGAGATCTTCCCGGTCGTGCTCACCTGTCTGAAGGACAAGGATGACGATGTGAAGAGAAACGGGTCTATCTTAGTGAGAGAAATCGCAAAACACTCACCCGAGCTCTCTCAACTGATAGTTAACCGAGGAGGAGTGGCTGCTGTGATTGATTGCATCAGCTCCTGCAAAGGGAGCATACGGCTGCCCGGCATCATGATGCTCGGCTATGTGGCGGCTCATTCCGAAAACCTCGCTATGGCTGTCATCATTTCCAAGGGTGTGCCCCAGTTGTCAATCTGCTTGTCAGAAGAGCCAGAAGATCACATTAAGGCCGCGACTGCTTGGTCCTTAGGACAGATTGGGAGACACACTTCAGAGCACGCCAAGGCTGTCGCGGTCACTAACACCCTGCCGGTCCTGCTGTCCTTGTACATGGCCGCGGAGAGCTCCGAGGACCTCCAGGTTAAGTGTGAGAAATCCATGAAGAACATCCTGCAGAAGTGCACCCACGTCCCCGCCCTGGAGCCCCTCCTGCATGACGCGCCCCCCAACATCCTGCTGACCTGTTTCTGTGCTTGGTTATTGCAGGTGCTGCCGCATGACAGCAAAGCTCGAAGATTTTTTGTTACAAGTGGTGGCCTCAAAAAGATTCAGCAAATAAAAGCAGAACCTGGCTCTCTCCTTCAAGAATATATCAATAAAATTAACAACTGCTACCCAGAAGAAATAGTAAGGTATTATTCCCCTGGATTTTCAGATGCACTTCTGCAGAGGGTGGACAGCTATCAACCTcctactaac

>Spag6_Human_gi27262639

ATGAGTCAGAGGCAGGTGCTGCAAGTGTTCGAGCAATACCAGAAGGCCAGGACCCAGTTCGTGCAGATGGTGGCGGAGCTGGCGACTAGACCCCAAAACATCGAGACGCTGCAGAACGCGGGTGTAATGTCTTTGCTGAGAACTCTTCTTCTGGACGTGGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTTGGGAGACTGGCCAATTATAATGATGACCTAGCAGAAGCTGTTGTGAAGTGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAGAACAGAATCGCTTCTACAAGAAAGCAGCTGCCTTTGTGTTACGAGCAGTTGGTAAACATTCTCCCCAGCTAGCTCAGGCAATAGTCGATTGTGGAGCACTGGATACGCTGGTCATATGCTTGGAAGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCCTGGGCACTTAGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGATGCAGGAGCTGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCTTCGGCCCTCAGTGATATTGCAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCTGTTGCTCATTTAGCCCAGATGATCCTGAACCCTGATGCTAAATTGAAGCATCAGATCCTTTCAGCTCTCAGTCAGGTTTCAAAACATTCCGTGGATCTGGCAGAAATGGTTGTTGAAGCAGAGATTTTTCCAGTTGTACTTACCTGTCTGAAGGACAAGGATGAATACGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAACATACACCCGAGCTTTCACAGCTGGTAGTTAACGCAGGAGGGGTTGCTGCCGTGATTGACTGCATTGGGTCCTGCAAAGGGAACACACGGCTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCTGAGAACCTAGCAATGGCAGTCATCATTTCTAAGGGTGTACCCCAGTTGTCAGTCTGCTTGTCAGAAGAACCGGAAGATCATATTAAGGCTGCAGCTGCTTGGGCCTTAGGACAGATTGGAAGACACACTCCTGAACACGCACGGGCTGTTGCAGTCACAAATACTTTGCCAGTTCTGCTTTCTTTGTACATGTCAACAGAAAGTTCTGAGGATCTCCAAGTAAAAAGTAAAAAAGCCATAAAGAATATCCTGCAAAAATGTACCTACTTACCAGCCCTTGAACCATTTCTATATGATGCTCCTCCCAATATTCTGAAACATGTGGTTGGACAGTTCAGTAAGGTGCTGCCGCATGATAGCAAAGCTCGACGACTTTTTGTAACAAGTGGTGGCCTTAAAAAAGTTCAAGAGATAAAAGCAGAACCTGGTTCTCTCCTTCAAGAATACATCAACAGTATTAACAGTTGTTACCCCGAGGAAATAGTGAGGTATTATTCCCCTGGATATTCAGATACACTTCTGCAGAGGGTGGACAGCTATCAACCACTTaataac

>Spag6_Dog_gi73948885

ATGAGCCAGAGGCAGGTGTTGCAAGTGTTCGAGCAGTACCAGAAGGCCCGGACCCAGTTCGTGCAGATGGTGGCGGAGCTGGCGACCAGACCCCAAAACATCGAGACGCTGCAGAACGCGGGTGTAATGTCTTTGCTGAGGCCTCTTCTTCTGGATGTGGTCCCGACGATTCAACAGACTGCTGCATTGGCTCTTGGGAGACTGGCTAATTATAATAATGACTTAGCAGAAGCAGTGGTGAAGGGTGACATTCTTCCCCAGCTTGTTTATTCATTGGCAGAACAGAATCGTTTTTACAAAAAAGCAGCTGCTTTTGTGTTACGAGCAGTTGGTAAACATTCTCCTCAACTAGCTCAGGCAATAGTTGATTGTGGAGCACTGGATACGCTGGTGATATGCTTGGAAGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCTTGGGCACTTGGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGATGCGGGAGCTGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGGTTGCTGCTTCGGCCCTCAGTGATATTTCAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGACGTGGGAGCCATTGCTCACTTGGCCCAGATGATCCTGAACCCTGATTCTAAATTAAAGCATCAGGTCCTTTCAGCTCTTAGTCAGATTGCAAAACATTCTGTGGATCTGGCAGAGATGGTGGTGGAAGCAGAGATCTTTCCAGTTGTACTTACCTGTCTGAAGGACAAAGATGAATACGTGAAGAAAAATGCTTCTACTCTAATTAGAGAGATTGCAAAACATACACCTGAGCTTTCACAGTTGATAGTTAACGCAGGAGGAGTGGCTGCCGTGATTGATTGTATCGGGTCCTGCAAAGGAAACATAAGACTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCTGAGAACCTGGCAATGGCAGTGATCATTTCCAAGGGTGTGCCTCAGTTGTCAGTCTGCTTGTCAGAAGAACCAGAAGATCATATTAAGGCTGCTGCTGCTTGGGCCTTAGGACAGATTGGGAGACACACTCCAGAGCACGCACGGGCTGTTGCGGTCACAAACACCTTGCCAGTTCTACTGTCATTGTACATGTCAACAGAAAGTTCTGAGGATCTTCAAGTAAAAAGTAAAAAAGCCATCAAGAATATCCTACAAAAATGCACCTATCTACCAGCCCTTGAGCCATTTCTGTATGATGCACCTCCCAATATTCTGAAGCATGTGGTTGGACAGTTCAGTAAGGTACTGCCACATGACAGCAAAGCTCGACGACTTTTTGTAACAAGTGGTGGACTTAAAAAGATTCAAGAGATAAAAGCAGAGCCTGAATCTCTCCTTCAAGAATACATCAACAGTATTAACAACTGTTACCCAGAGGAAATAGTAAGATATTATTCCCCTGGATATTCAGACACACTTCTGCAGAGAGTGGACAGCTATCAACCAATTactaac

>Spag6_Cow_gi84000338

ATGAGTCAGAGGCAGGTGCTGCAAGTGTTCGAGCAGTACCAGAAAGCCCGGACCCAGTTCGTCCAGATGGTGGCGGAACTGGCGACCAGACCCCAGAACATCGAGACGCTGCAGAACGCGGGCGTAATGGCTTTGCTGAGGCCTCTTCTTCTGGATGTAGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTGGGGAGACTGGCTAATTATAATGATGACCTGGCAGAAGCAGTCGTGAAGGGTGATATTCTTCCACAGCTTGTTTATTCATTGGCAGAACAGAATCGTTTTTACAAGAAAGCAGCTGCCTTTGTATTACGAGCGGTTGGTAAACATTCTCCCCAGCTAGCTCAGGCAATAGTTGATTGTGGAGCGCTGGACACACTGGTGATTTGCTTGGAAGATTTTGATCCTGGAGTCAAAGAGGCTGCAGCCTGGGCACTTGGCTATATTGCAAGACATAATGCAGAGCTGTCACAAGCTGTGGTGGATGCGGGAGCTGTTCCTCTCTTAGTGCTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGTTTCAGCCCTCAGTGATATTGCAAAGCATTCTCCAGAGTTAGCACAGACAGTGGTGGATGCAGGAGTGATTGCTCACTTAGCCCAGATGATCCTGAACCCTGATGCTAAACTGAAGCGTCAGGTCCTTTCAGCTCTCAGTCAGATCGCGAAACATTCTGTGGATCTGGCAGAAATGGTGGTTGAAGCAGAGATTTTTCCAGTGGTACTTACCTGTCTGAAGGACAAAGATGAATACGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAACATACACCCGAGCTTTCTCAGCTGATAGTGAACGCTGGGGGAGTTGCTGCTGTGATCGACTGCATCGGGTCCTGCAAAGGGAACATAAGGCTGCCAGGCATCATGCTGCTTGGCTATGTGGCAGCTCATTCTGAGAACCTGGCAATGGCGGTCATCATTTCCAAGGGTGTGCCCCAGTTGTCCCTCTGCTTGTCAGAAGAACCAGAAGACCATATTAAGGCTGCTGCTGCCTGGGCCTTGGGACACATTGGGAGACACACTCCTGAGCACGCACGGGCCGTCGCTGTCACAAACACTCTGCCTGTCCTGCTCTCTTTGTACATGTCAACCGAGAGTTCTGAGGATCTTCAAGTGAAAAGTAAAAAAGCCATAAAGAATATCTTACAAAAATGCACCTATTTACCAGCTCTTGAACCATTTCTGTATGATGCTCCTCCCAATATTCTGAAGCACGTGGTTGGACAATTCAGTAAGGTGCTGCCACATGATAGCAAAGCTCGACGACTTTTTGTAACAAGTGGTGGACTTAAAAAGGTGCAAGAGATAAAAGCAGAACCTGGATCTCTCCTTCAGGAATATATCAACAGCATTAACAACTGTTACCCAGAAGAAATAGTAAGGTATTATTCCCCTGGATATTCAGAAACACTTCTGCAGAGAGTGGACAGCTATCAACCACTTactaac

>Spag6_Opossum_gi126341261

ATGAGCCAAAGGCAGGTGCTCCAAGTGTTCGAACAGTACCAGAAGGCTCGGACTCAGTTCGTGCAGATGGTGGCAGAGTTGGCAACCAGACCCCAAAACATTGAGACGCTACAAAATGCAGGTGTAATGTCTTTGCTGAGGCCGCTTCTCTTGGATGTTGTCCCAACTATTCAGCAGACTGCTGCCCTGGCTCTTGGGCGACTGGCTAATTATAACGATGACTTGGCAGAAGCTGTCGTAAAGGGAGACATTCTTCCGCAGCTCGTTTATTCATTGGCGGAACAGAATCGTTTCTACAAGAAAGCAGCAGCATTTGTACTAAGAGCTGTTGGCAAACATTCTCCTCAGTTAGCTCAAGCAATAGTTGATTGTGGAGCATTGGATACATTGGTTGTATGCTTGGAAGATTTTGACCCAGGAGTAAAGGAAGCATCAGCTTGGGCCCTTGGGTACATTGCAAGACATAATGGAGAACTGTCGCAAGCTGTGGTGGATGCAGGAGCAGTTCCCCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCCTCAGCTCTCAGCGATATTTCCAAGCATTCTCCAGAGTTAGCCCAGACAGTGGTGGATGCAGGAGCTATTGCTCACCTAGCACAGATGATCCTGAACCTTGATGCTAAACTGAAGCGTCAAGTGCTTTCTGCTCTTAGTCAGATAGCAAAGCATTCGGTGGATCTGGCAGAAATGGTTGTCGAAGCAGAAATTTTCCCTGTGGTTCTTACATGCCTAAAGGACAAAGATGAATACGTCAAGAAAAATGCTTCAACTCTAATTAGAGAAATAGCAAAACATACGCCTGAGCTTTCACAACTAATAGTTAATGCAGGAGGAGTTGCTGCTGTGATTGATTGTATTGGTTCCTGCAAAGGAAATATAAGGCTTCCTGGCATTATGATGCTTGGTTATGTAGCTGCTCATTCTGAGAACCTCGCCATGGCAGTGATCATCTCCAAGGGAGTGCCCCAGTTGGCAATCTGCTTGTCAGAAGAATCAGAAGATCATATTAAGGCTGCTGCTGCTTGGGCCTTAGGACAGATTGGAAGGCACACTCCTGAGCACGCACGTGCTGTGGCTGTGACAAATGCCTTGCCGATTCTACTGAGTCTCTACATGTCACAAGACAGTTCAGAGGATCTTCAAATAAAAAGTAAAAAAGCTATTAAAAATATTCTCCAAAAATGCACCTATTTGCCAGCCCTTGAGCCATTCTTGTATGATGCTCCTCCGAATATTTTGAAGCATGTTGTTGGACAGTTCAGTAAGGTGCTGCCCCATGATAGCAAAGCACGGAGACTTTTTGTTACAAGTGGTGGTCTTAAAAAGGTTCAAGAGATAAAGGCAGAACCTGGTTCTCTTCTTCAAGAATACATCAACAATATTAACAATTGTTACCCAGAGGAAATAGTGAGGTATTATTCCCCTGGATATTCAGAGACACTTTTGCAGAGAGTGGACAGCTATCAGCCTCTTgta

>Spag6_Elephant_ENSLAFT00000005191

ATGAGCCAGAGGCAGGTGCTGCAAGTGTTCGAGCAATACCAGAAAGCTCGGACCCAGTTCGTGCAGATGGTGGCGGAGCTGGCGACGAGACCTCAAAACATCGAGACGCTGCAGAACGCGGGTGTAATGTCTTTGCTGAGGCCTCTTCTTCTGGATGTGGTCCCAACAATTCAACAGACTGCAGCTTTGGCTCTTGGGAGACTGGCTAATTATAATGATGACCTAGCAGAAGCAGTTGTGAAGGGTGATATTCTTCCACAGCTTGTTTATTCACTGGCAGAACAGAATCGCTTTTACAAGAAAGCAGCTGCTTTTGTGTTGCGAGCAGTTGGTAAACATTCTCCTCAACTAGCTCAGGCAATTGTTGATTGTGGAGCACTGGATACACTGGTGATATGCTTGGAAGATTTTGACCCTGGAGTCAAGGAGGCTGCAGCCTGGGCTCTTGGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGGTGGATGCGGGAGCGGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCTTCGGCCCTCAGTGATATTTCGAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCTATTGCCCACTTAGCCCAGATGATCCTGAATCCTGATGCTAAATTGAAGCGTCAGGTCCTTTCAGCTCTCAGCCAGGTAGCAAAGCATTCTGTGGATCTGGCGGAGATGGTGGTTGAAGCAGAGATTTTTCCAGTTGTACTTACATGTCTGAAGGACAAAGATGAATATGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAACATACACCCGAGCTTTCACAGCTGATAGTTAATGCAGGAGGAGTTGCTGCTGTGATTGACTGTATTGGCTCCTGCAGAGGAAATATAAGGCTGCCTGGCATCATGATGCTGGGTTATGTGGCGGCTCACTCTGAGAACCTGGCCATGGCGGTGATCATTTCCAAGGGTGTACCCCAGTTGTCAGTCTGCTTGTCAGAAGAACCAGAGGATCATATTAAGGCTGCTGCTGCTTGGGCCTTAGGACAGATTGGGAGACACACTCCTGAACACGCACGGGCTGTTGCAGTCACAAATACTTTGCCTGTTCTGCTTTCTTTGTACATGTCAACAGAAAGTTCTGAGGACCTTCAAGTAAAAAGTAAAAAAGCTATAAAGAATATCCTTCAAAAATGTACCTATCTACCAGCTCTTGAACCGTTTCTGTATGATGCTCCCCCCAACATTCTGAAGCATGTGGTTGGACAGTTCAGTAAGGTGCTGCCACATGATAGCAAAGCACGACGACTTTTTGTAACAAGTGGTGGACTTAAAAAAGTTCAAGAGATAAAAGCAGAACCTGGATCTCTCCTTCAAGAATACATCAACAGTATTAATAATTGTTACCCAGAGGAAATAGTAAGGTATTATTCCCCTGGATATTCAGATACACTCCTGCAGAGGGTGGACAGCTATCAACCACTTATTAAC

>Spag6_Chicken_ENSGALT00000012816

ATGAGCCAGCGGCAGGTCCTGCAAGTGTTCGAGCAGTACCAAAGCGCCCGGACGCACTTCGTGCAGGCGGTGGCCGAGCTGTCGGCGAGGCCGCAGAACATCGGGACGCTGCGGGAGGCAGGTGTAATATCTTTGCTGAGACCTCTTTTGCTGGATGTGGTCCCAACTATCCAACAGACTGCTGCTTTGGCTCTTGGGAGGCTTGCCTATTTTAACGATGACCTGGCAGAAGCAGTCGTGAAGGAAGACATTCTTCCACAGCTCGTGTGTTCCTTGTCTGAGCAGAATCGTTTCTACAAGAAAGCAGCTGCATTTGTGCTAAGAGCAGTTGGGAAACATTCTCCACAGCTAGCTCAGGCAATAGTTGAGTGTGGAGCACTGGAAGCACTTGTGATTTGCTTGGAAGATTTTGATCCTGGAGTGAAAGAAGGTGCAGCTTGGGCACTCGGGTATATTGCCCGACACAATTCAGAACTGTCACAAGCTGTGGTGGATGCAGGAGCCGTTCCTCTTTTAGTGCTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGCTGCTTCAACTCTTAGTGATATTTCAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCTATCGCTTACTTAGCTCAGATGATCCTGAACCCCGATGCTAAACTGAAGTGTCAGGTGCTGTCAGCGCTAAGCCAAATAGCAAAGCATTCGGTGGATCTTGCAGAGTTGGTGGTTGAAGCAGAAATTTTCCCAGTTGTGCTCACATGCATGAAGGACTCGGATGAATATGTCAAGAAAAATGGTGCAACTTTAATTAGAGAGATAGCAAAGCATTCGCCTGAGCTTTCACAGTTTATAGTAAATGCAGGTGGAGTTGCTGCTGTGATTGATTGTATTGGCAGCTGCAAAGGGACTGTCAGACTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCAGAGAACCTGTCAATGGCAGTTATTGTCTCCAAGGGAATACCACCACTGTGTACCTGTTTGTTAGAAGAACATGAAGATCATATTAAGGCAGCAGCTGCGTGGGCCTTGGGACAGATTGGAAGACATACTCCTGAGCATGCACGTGCTGTAGCAGAAACAAATGTGCTAGGCACACTGCTTTCTATGTATATGGACATACGCAGTTCAGAAGATCTTCAGCTGAAAGCTAAAAGAGCCTTAAAGAACATCCTTCAGAAATGCACTTATCTTCCAGCACTTGAACCACTGCTGCATGATGCCCCTCCCAATATTATGAAACACATTGTTGGGCAGTTTAGCAAGGTGTTACCACATGACAGTAAAGCACGTCGTCTCTTTGTAACATCTGGTGGGCTTAAAAAGGTTCAAGAAATAAAAGCAGAACCCGGGTCACTTCTTCAGGAGTATATCAACACTATTAACAATTGCTATCCAGAGGAAGTAGTAAGGTATTATTCCCCTGGATATTCTGAGATGCTTCTGGAACGGGTGGAAAATTACCAACCAGTTTTATAA

>Spag6_Lizard_ENSACAT00000009029

GTCTTTGAGCAATATCAAAGAGCCCGGACACAGTTTGTGCAGGCCGTTGCAGAACTTGCAACTAGGCCACAAAATATCGAAACACTTCAGAATGCAGGTGTAATGGCTCTGCTGAGGCCGCTCCTATTGGACGTGGTTCCAAATATTCAACAGACAGCTGCCATGGCCCTCGGGAGACTTGCCAATTATAACAATGACCTGGCAGAAGCTGTAGTTAAGGGAGATATTCTTCCACAGCTTGTGTATTCATTGGCTGAGCAAAATCGTTTCTACAAGAAAGCTGCTGCATTTGTATTAAGAGCAGTTGCAAAACATTCCCCTCAACTAGCCCAGGCTATAGTCAACTGTGGAGCACTAGATACATTGGTGATTTGCTTGGAAGATTTTGATCCTGGTGTAAAAGAGGCTGCTTCCTGGGCTCTTGGCTACATAGCAAGACATAATGCAGAACTGTCACAGGCGGTGGTAGATGCTGGAGCCATTCCTTTCTTAGTCCTCTGTATCCAGGAGCCAGAGACTGCTTTGAGAAGGATTGCTGCCTCCGCGCTCAGTGATGTTTCAAAGCATTCTCCAGAGTTAGCCCAGACCGTCGTGGATGCAGGGGCAATTGCTCACTTGGCTCAAATGATCTTCAACCCGGATGCCAAACTGAAGCGTCAGGTGCTATCAGCTCTCAGCCAGATAGCAAAGCATTCTGTGGATCTGGCAGAAATGGTGGTTGAAGCAGAAATTTTCCCTGTTGTGCTCACATGCCTGAAGGACTCCGATGAATATGTCAAGAAAAACGCTGCAACTCTAATTAGAGAGATAGCAAAGCATACGCCTGAGCTTTCACAACTTATCGTGAATGCAGGGGGAGTTGCTGCTGTCATTGACTGTATTGGCTGCTGCAAAGGAAATGTTAGACTCCCAGGGATTATGATGCTGGGCTATGTGGCAGCTCATTCAGAGAACCTTGCCATGGCAGTGATACTCTCCAAGGCGATTCCACAGCTGGCAGTTTGCTTGTCTGAAGAGCCAGAAGATCATATTAAAGCAGCGGCTGCATGGTCTTTGGGACAGATTGGGAGACACACTCCAGAACATGCTCGGGCTGTTGCAGTAGCAAACGTGTTACCCGTGTTACTTTCTTTCTATATGGCAAGTGAGAGTGCAGAAGACCTTCAGGTCAAAACAAAGAAGGCTTTGAAAAACATCTTACAAAATTGCACTCATCTGCCATCACTTGAACCTTTGCTCTATGATGCTCCTCCCAACATCCTGAAACACGTAGTTGGCCAGTTCAGTAAAGTTCTGCCACATGATCCCAAAGCTAGACGCCTTTTTGTAACAAGTGGTGGCCTTAAAAAAGTGCAGGAGATAAAAGCAGAGGCTGGGTCCCTGCTTCAAGAATACATCAACACAATTAACAATTGTTATCCTGAAGAAATAGTCAGATATTATTCCCCTGAATATTCTGAAGCACTTCTGGAAAGAGTAGAAAATTATCAGCCTCTTAACAAG

>Spag6_Frog_ENSXETT00000016084

ATGAGCCAGCGCCAGGTTCTTCAGGTTTTTGAGCAGTATCAGCGGGCCAGGACCCAGTTTGTCCAGACAGTTGCAGAGCTTGCTACAAGGCCTCAGAACATCGAAACATTGCAGAATGCAGGCGTTATGTCTCTGCTACGGCCCCTTCTCTTGGACGTGGTCCCAACAATCCAGCAAACCTCTGCTTTGGCTCTCGGGAGACTTGCCAATTACAACGATGATCTGGCAGAAGCCGTTGTTAAAGGAGACATTCTTCCCCAGCTTGTGTATTCTCTGGCTGAACAGAACCGCTTTTACAAGAAGGCAGCAGCCTTTGTGCTACGAGCTGTTGCCAAACACTCTCCTCAGTTAGCTCAGTCAATAGTGGACTGTGGAGCGGTGGATACGCTGGTGATTTGCCTGGAGGACTTTGACCCTGGAGTGAAGGAGTCAGCAGCTTGGGCACTGGGCTATATTTCAAGACACAATGCAGAACTGTCACAGGCCGTGGTGGATGCGGGAGCTGTTCCCTTGCTCGTCCTCTGTATCCAGGAGCCAGAAATCGCCCTGAAAAGGATTGCTGCCTCTGCCTTGAGTGATCTATCAAAGCATTCTCCTGAGCTCGCCCAGACCGTTGTGGATGCAGGGGCAATAGCCCACTTAGCTCAGATGATTCTCAATCCAGATGCTAAACTCAAGCGCCAGGTGCTGTCTGCCCTAAGCCAAATTGCTAAGCATTCCGTTGACCTGGCGGAAATGGTGGTAGAAGCCGAAATCTTCCCTGTCGTCTTGACGTGTCTGAAGGACCCTGATGAATATGTTAAGAAAAATTCATCTACGCTGATCCGAGAAATTGCACGGCACACCCCCGAGCTCTCTCAGTTGATAGTGAATGCGGGAGGAGTGGCTGCTGTCATTGATTGCATTGGAAATTGCAGCGGGAATGTGAGGCTTCCCGGCATCATGATGCTGGGTTATGTGGCAGCTCATTCTGAGAACCTGGCAATGGCCGTCATTATCTCAAAGGGGGTACCACAATTAGCCATCTGCCTGTCAGAGGAAGCAGAAGATCACATAAAGGCAGCTGCTGCCTGGTCGCTGGGCCAGATTGGAAGACACACTCCAGAGCATGCACGTGCTGTTGCTGTTGCCAATGTATTGCCAAAGCTGCTTTCCCTATACATGGACACAGACAGCTCCGAGGACCTCCAAGTGAAGGCAAAGAAGGCCTTAAAGAACATACTGCAGAAGTGTACCCATCTGCCAGCGCTGGAGCCCTTGCTGCACGATGCACCGCCTAATATCCTGAAGCATGTGGTCGGGCAGTTCAGCAAGGTTCTTCCACACGACAGCAAAGCAAGGCGACTATTTGTCACAAGTGGGGGGCTGAAAAAGATTCAAGAGATAGTGGCAGACACGGGCTCACCCCTCCAAGAATACATCAACACCATCAACAACTGTTTTCCTGAAGAGATTGTCAGATACTATTCTCCCGGATACTCCCAGTCACTTCTAGAAAGGGTGGATCACTACCAGCCAGTTATTTAA