Supplementary Information

Mitochondrial Genomes and Exceptional Longevity in a Chinese Ppopulation: the Rugao Llongevity Sstudy

Lei Li, Hong-Xiang Zheng, Zuyun Liu, Zhendong Qin, Fei Chen, Degui Qian, Jun Xu, Li Jin, Xiaofeng Wang

Supplementary Table S1. The prevalence of major haplogroups in EL subjects and control subjects.

Haplogroups / EL (%)
N=237 / Control (%)
N=444 / P-value
R11 / 4 (1.69) / 4 (0.90) / 0.46
B4 / 28 (11.81) / 57 (12.84) / 0.81
R9 / 5 (2.11) / 5 (1.12) / 0.33
D6 / 0 (0) / 1 (0.23) / 1.00
M33 / 1 (0.42) / 1 (0.23) / 1.00
Z / 6 (2.53) / 13 (2.93) / 0.76
M9 / 0 (0) / 9 (2.03) / 0.03 *
C / 12 (5.06) / 14 (3.15) / 0.22
M10 / 5 (2.11) / 4 (0.90) / 0.29
A / 18 (7.59) / 28 (6.31) / 0.52
M12 / 0 (0) / 3 (0.68) / 0.56
F / 29 (12.24) / 62 (13.96) / 0.56
G / 13 (5.49) / 19 (4.28) / 0.57
N9 / 15 (6.33) / 33 (7.43) / 0.64
D4 / 40 (16.88) / 71 (15.99) / 0.83
B5 / 13 (5.49) / 22 (4.95) / 0.86
D5 / 16 (6.75) / 33 (7.43) / 0.88
M7 / 22 (9.28) / 40 (9.01) / 0.89
M8 / 4 (1.69) / 13 (2.93) / 0.44
Other / 6 (2.53) / 12 (2.7) / 0.89

Note: “*” denotes p<0.05.


Supplementary Table S2. The distributions of private mtDNA functional mutations in 13 protein-coding genes, rRNA and tRNA genes in EL and control subjects.

Region/gene / ELa
(n=237) / Control
(n=444) / P-value / ELb
(N/S) / Control
(N/S) / P-value
ATP / 13 / 38 / 0.18 / 13/16 / 38/26 / 0.19
ATP6 / 9 / 33 / 0.08 / 9/11 / 33/19 / 0.15
ATP8 / 4 / 5 / 0.73 / 4/5 / 5/7 / 1
COX / 30 / 44 / 0.33 / 30/46 / 44/88 / 0.37
COX1 / 16 / 15 / 0.06 / 16/18 / 15/40 / 0.06
COX2 / 5 / 10 / 1 / 5/17 / 10/21 / 0.54
COX3 / 9 / 19 / 0.77 / 9/11 / 19/27 / 0.78
Cytb / 14 / 34 / 0.43 / 14/30 / 34/47 / 0.27
ND / 53 / 92 / 0.67 / 53/95 / 92/219 / 0.18
ND1 / 13 / 18 / 0.42 / 13/12 / 18/36 / 0.11
ND2 / 7 / 18 / 0.48 / 7/17 / 18/45 / 0.96
ND3 / 4 / 3 / 0.25 / 4/2 / 3/11 / 0.12
ND4 / 8 / 15 / 0.99 / 8/14 / 15/45 / 0.31
ND44L L / 0 / 1 / 1 / 0/4 / 1/7 / 1
ND5 / 17 / 32 / 0.99 / 17/38 / 32/53 / 0.41
ND6 / 4 / 5 / 0.73 / 4/8 / 5/22 / 0.42
All proteins / 110 / 208 / 0.95 / 110/187 / 208/380 / 0.63
12s RNA / 19 / 33 / 0.8 / -- / -- / --
16s RNA / 17 / 36 / 0.68 / -- / -- / --
rRNA / 36 / 69 / 0.92 / -- / -- / --
tRNA / 32 / 48 / 0.36 / -- / -- / --

Note: “a” represents that the total number of private nonsynonymous or functional mutations in genes and regions were compared between two groups; “b” represents that the nonsynonymous/synonymous ratios (N/S) of private mutations in protein-coding genes were compared between two groups.