Supplementary information

Supplementary Fig. 1 NucleicacidanddeducedaminoacidsequenceofDcHsf10

(* represents the stop codon)

Supplementary Fig. 2 Nucleic acid and deduced amino acid sequence of DcHsf 09

(* represents the stop codon)

Supplementary Fig. 3 Nucleic acid and deduced amino acid sequence of DcHsf 01

(* represents the stop codon)

Supplementary Fig. 4 Nucleic acid and deduced amino acid sequence of DcHsf 02

(* represents the stop codon)

Supplementary Fig. 5 Nucleic acid and deduced amino acid sequence of DcHsf 16

(* represents the stop codon)

Supplementary Fig. 6 Comparison of expression levels of five DcHsf genes in four different stresses

(a Expression patterns at 38°C. b Expression patterns at 4°C. c Expression patterns under high salt treatment. d Expression patterns under drought stress. A DcHsf 10; B DcHsf 09; C DcHsf 01; D DcHsf 02; E DcHsf 16)

Supplementary Table 3 Description of DcHsf genes in quantitative real-time PCR

K respresents ‘Kurodagosun’, J represents ‘Junchuanhong’

Gene / The number of base (bp) / Forward primer (5'-3' )forword/reverse / Efficiency (E%) / Correlation coefficients(R2)
K / J / K / J
DcHsf 01 / 909 / ATCTGATGCCGTTGAAGCGGTTATG
ATCAACACCTTCACCACTATTATCC / 106.4 / 99.6 / 1.000 / 0.999
DcHsf 02 / 990 / TTCAGTTCCGACTACTAACACAGAG
TCATCACAGTACCGCTTCAAAGGCA / 104.0 / 101.6 / 0.994 / 1.000
DcHsf 09 / 1443 / TTCAGCTAAGCTCGCCCAGGACAGT
TGGTTTTTAGTTGCTGTATCATGTA / 100.3 / 95.7 / 0.999 / 0.999
DcHsf 10 / 1212 / CTTGAAAGAACAAGCGATTACTAGC
TGATTATTTACACTCCCCATACTCC / 100.6 / 100.7 / 0.999 / 0.994
DcHsf 16 / 939 / GCGTGGTTGATGGGTCAGAGAAGG
AGCAGTGAGAAAGGATAGGGCAGTA / 100.3 / 101.1 / 0.998 / 1.000
Tubulin / 1302 / GAGTGGAGTTACCTGCTGCCTTC ATGTAGACGAGGGAACGGAATCAAG / 105.5 / 102.8 / 0.998 / 0.998

Supplementary Table 1 HSF transcription factors in Arabidopsis, Vitis vinifera, and Oryza sativa

AtHSF / GeneID / Subfamily / VvHSF / Gene-ID / Subfamily / OsHSF / Gene-ID / Subfamily
AtHSF01 / At4g17750 / HSFA1a / VvHSF01 / GSVIVT01002066001 / HSFB1 / OsHSF01 / Os01g39020.1 / HSFA2
AtHSF02 / At5g16820 / HSFA1b / VvHSF02 / GSVIVT01003118001 / HSFA7 / OsHSF02 / Os01g43590.1 / HSFC
AtHSF03 / At1g32330 / HSFA1d / VvHSF03 / GSVIVT01009477001 / HSFB4 / OsHSF03 / Os01g53220.1 / HSFC
AtHSF04 / At1g2990 / HSFA1e / VvHSF04 / GSVIVT01010595001 / HSFB2 / OsHSF04 / Os01g54550.1 / HSFA4
AtHSF05 / At1g26150 / HSFA2 / VvHSF05 / GSVIVT01011632001 / HSFA8 / OsHSF05 / Os02g13800.1 / HSFC
AtHSF06 / At5g03720 / HSFA3 / VvHSF06 / GSVIVT01013107001 / HSFA5 / OsHSF06 / Os02g29340.1 / HSFA5
AtHSF07 / At4g18880 / HSFA4a / VvHSF07 / GSVIVT01015183001 / HSFA2 / OsHSF07 / Os02g32590.1 / HSFA3
AtHSF08 / At5g45710 / HSFA4c / VvHSF08 / GSVIVT01015389001 / HSFC / OsHSF08 / Os03g06630.1 / HSFA7
AtHSF09 / At4g13980 / HSFA5 / VvHSF09 / GSVIVT01018041001 / HSFA7 / OsHSF09 / Os03g12370.1 / HSFA8
AtHSF10 / At5g43840 / HSFA6a / VvHSF10 / GSVIVT01019829001 / HSFB2 / OsHSF10 / Os03g25120.1 / HSFB4
AtHSF11 / At3g22830 / HSFA6b / VvHSF11 / GSVIVT01020781001 / HSFA4 / OsHSF11 / Os03g53340.1 / HSFA7
AtHSF12 / At3g51910 / HSFA7a / VvHSF12 / GSVIVT01021040001 / HSFA5 / OsHSF12 / Os03g58160.1 / HSFA7
AtHSF13 / At3g63350 / HSFA7b / VvHSF13 / GSVIVT01021269001 / HSFA4 / OsHSF13 / Os03g63750.1 / HSFA1
AtHSF14 / At1g67970 / HSFA8 / VvHSF14 / GSVIVT01022134001 / HSFB1 / OsHSF14 / Os04g48030.1 / HSFB3
AtHsf15 / At5g54070 / HSFA9 / VvHSF15 / GSVIVT01024378001 / HSFA1 / OsHSF15 / Os05g45410.1 / HSFA4
AtHSF16 / At4g36990 / HSFB1 / VvHSF16 / GSVIVT01033257001 / HSFB3 / OsHSF16 / Os06g35960.1 / HSFC
AtHSF17 / At5g62020 / HSFB2a / VvHSF17 / GSVIVT01033781001 / HSFA3 / OsHSF17 / Os06g36930.1 / HSFA2
AtHSF18 / At4g11660 / HSFB2b / VvHSF18 / GSVIVT01035385001 / HSFA2 / OsHSF18 / Os07g08140.1 / HSFA7
AtHSF19 / At2g41690 / HSFB3/B3a / VvHSF19 / GSVIVT01037333001 / HSFB4 / OsHSF19 / Os07g44690.1 / HSFB4
AtHSF20 / At1g46264 / HSFB4/4a / OsHSF20 / Os08g36700.1 / HSFB4
AtHSF21 / At3g24520 / HSFC1/1a / OsHSF21 / Os08g43334.1 / HSFB2
OsHSF22 / Os09g28200.1 / HSFB4
OsHSF23 / Os09g28354.1 / HSFB1
OsHSF24 / Os09g35790.1 / HSFB2
OsHSF25 / Os10g28340.1 / HSFA7

Supplementary Table 2 HSF transcription factors in Brassica rapa

BrHSF / GeneID / Subfamily / BrHSF / Gene-ID / Subfamily / BrHSF / Gene-ID / Subfamily
BrHSF01 / Bra000235 / B3 / BrHSF19 / Bra013409 / A9 / BrHSF37 / Bra025611 / A9
BrHSF02 / Bra000557 / A2 / BrHSF20 / Bra013412 / A9 / BrHSF38 / Bra025635 / A9
BrHSF03 / Bra000749 / B2 / BrHSF21 / Bra014054 / B1 / BrHSF39 / Bra029226 / A9
BrHSF04 / Bra001071 / A1 / BrHSF22 / Bra015050 / C / BrHSF40 / Bra029228 / A9
BrHSF05 / Bra001885 / A6 / BrHSF23 / Bra016994 / A9 / BrHSF41 / Bra029291 / B2
BrHSF06 / Bra002882 / A9 / BrHSF24 / Bra016996 / A9 / BrHSF42 / Bra029292 / B2
BrHSF07 / Bra004272 / A8 / BrHSF25 / Bra016998 / A9 / BrHSF43 / Bra030021 / A9
BrHSF08 / Bra007739 / A7 / BrHSF26 / Bra017595 / A4 / BrHSF44 / Bra030341 / A9
BrHSF09 / Bra008593 / A1 / BrHSF27 / Bra017800 / B1 / BrHSF45 / Bra032023 / A1
BrHSF10 / Bra009515 / A3 / BrHSF28 / Bra020988 / A4 / BrHSF46 / Bra032752 / A5
BrHSF11 / Bra010049 / B2 / BrHSF29 / Bra021381 / A1 / BrHSF47 / Bra033913 / A6
BrHSF12 / Bra011735 / B1 / BrHSF30 / Bra022949 / A9 / BrHSF48 / Bra035507 / A1
BrHSF13 / Bra012571 / A4 / BrHSF31 / Bra023258 / A1 / BrHSF49 / Bra035993 / A6
BrHSF14 / Bra012828 / A7 / BrHSF32 / Bra023584 / A1 / BrHSF50 / Bra040179 / A1
BrHSF15 / Bra012829 / A7 / BrHSF33 / Bra023800 / A6 / BrHSF51 / Bra040634 / B1
BrHSF16 / Bra013253 / C / BrHSF34 / Bra024228 / A9 / BrHSF52 / Bra040968 / B2
BrHSF17 / Bra013358 / A4 / BrHSF35 / Bra025606 / A9
BrHSF18 / Bra013406 / A9 / BrHSF36 / Bra025608 / A9

Supplementary Table 4 Summary information of the HSF transcription factors in Daucus carota

DBD: DNA-binding domain, NLS: nuclear localization signal, NES: nuclear export signal.

DcHSF / GeneID / Subfamily / DBD / NLS / NES / Molecular weight / Theoretical pI / Ratio of negative amino acid / Ratio of positive amino acid / Instability
index / Aliphatic
index / GRAVY
DcHSF01 / Dck41808 / B2 / 20-113 / - / (115)LANLTV / 34045.0 / 5.04 / 12.3 / 15.2 / 58.57 / 71.69 / -0.701
DcHSF 02 / Dck02400 / B2 / 20-113 / 102-GEKRLLR- 108 / (249)LKR / 36376.3 / 4.98 / 11.6 / 14.3 / 57.74 / 69.39 / -0.706
DcHSF 03 / Dck04021 / A4 / 10-103 / 94 - RHLLPKIHRRKPI - 106 / (248)LRVLER / 49104.3 / 5.52 / 9.7 / 13.2 / 46.64 / 65.77 / -0.716
DcHSF 04 / Dck04025 / A4 / 10-103 / 94-RHLLPKIHRRKPI-106 / (248)LRVLER / 49104.3 / 5.52 / 9.7 / 13.2 / 46.64 / 65.77 / -0.716
DcHSF 05 / Dck24852 / A2 / 50-143 / 138-KSIKRRRNVLRG-149 - / - / 43770.3 / 4.91 / 10.9 / 15.5 / 63.37 / 68.65 / -0.616
DcHSF 06 / Dck23336 / A7 / 38-131 / 228 - KKRRRP - 233 / (275)LDVLALEM / 40301.0 / 5.15 / 12.7 / 16.6 / 56.72 / 65.56 / -0.828
DcHSF 07 / Dck34576 / B1 / 4-97 / 246 - KKKRAR - 251 / - / 31429.2 / 6.17 / 13.8 / 14.5 / 37.70 / 70.25 / -0.674
DcHSF 08 / Dck08439 / B4 / 22-115 / - / (298)LENDDLGLNL / 35856.2 / 7.36 / 9.6 / 9.6 / 55.33 / 64.47 / -0.682
DcHSF 09 / Dck07781 / A5 / 11-104 / 103 - RRK - 105 / (197)SIDL / 53762.3 / 5.48 / 10.8 / 14.2 / 63.00 / 69.69 / -0.755
DcHSF 10 / Dck08996 / A4 / 10-103 / 208 - RKRRLPRK - 215 / (141)FEKDSLYL / 45922.7 / 5.31 / 10.7 / 15.2 / 53.22 / 65.58 / -0.817
DcHSF 11 / Dck08999 / A4 / 10-103 / 208 - RKRRLPRK - 215 / (141)FEKDSLYL / 45922.7 / 5.31 / 10.7 / 15.2 / 53.22 / 65.58 / -0.817
DcHSF 12 / Dck37366 / A2 / 52-144 / 139 - KTIKRRR - 145 / (244)IEIGRKRRLT / 42592.7 / 5.01 / 11.8 / 16.4 / 51.51 / 71.10 / -0.703
DcHSF 13 / Dck00022 / A2 / 25-118 / 113 - KTIKRRR - 119 / - / 39979.7 / 4.78 / 10.4 / 15.5 / 61.25 / 72.99 / -0.637
DcHSF 14 / Dck22869 / A4 / 10-103 / 101 - HRRKP - 105 / - / 33729.5 / 5.80 / 12.4 / 15 / 54.67 / 67.47 / -0.745
DcHSF 15 / Dck76119 / A7 / 38-131 / 126 - RNIRRRKA - 133 / (285)LDVLALEI / 41547.5 / 5.25 / 12.6 / 16.5 / 46.47 / 68.54 / -0.775
DcHSF 16 / Dck13183 / C / 9-102 / 184 - RIGEKKR - 190 / (130)LKQEQKILDL / 35410.9 / 5.47 / 11.8 / 13.8 / 67.18 / 69.36 / -0.576
DcHSF 17 / Dck15270 / A3 / 45-138 / 229 - KGG - 231 / (158)IEGEIEL / 28956.7 / 6.38 / 13.4 / 14.6 / 62.99 / 71.86 / -0.769
DcHSF 18 / Dck05653 / A7 / 38-131 / 126 - KQIKRRKPP - 134 / - / 37802.7 / 5.72 / 12.2 / 14.3 / 46.62 / 78.01 / -0.571
DcHSF 19 / Dck32039 / A4 / 12-105 / 210 - RKRRLPRK - 217 / (191)LKKPALALDL / 45780.1 / 5.37 / 12 / 15 / 54.93 / 73.17 / -0.750
DcHSF20 / Dck51191 / A4 / 12-105 / 210 - RKRRLPRK - 217 / (191)LKKPALALDL / 45780.1 / 5.37 / 12 / 15 / 54.93 / 73.17 / -0.750
DcHSF 21 / Dck78921 / A9 / 45-138 / 146 - KRNR - 149 / (179)VQEL / 39168.8 / 5.05 / 10.9 / 15.6 / 43.73 / 71.41 / -0.692
DcHSF 22 / Dck24257 / B1 / 13-99 / - / (94)LQIQRRRIENRSL / 31036.9 / 8.64 / 14.6 / 13.2 / 38.98 / 72.78 / -0.658
DcHSF 23 / Dck39090 / A7 / 1-83 / 188 - SKKRSKAS - 195 / - / 22707.2 / 9.93 / 18.3 / 11.7 / 37.98 / 80.61 / -0.626
DcHSF 24 / Dck00147 / A3 / 21-141 / - / - / 48436.7 / 5.30 / 10 / 13.3 / 53.68 / 77.61 / -0.421
DcHSF 25 / Dck63626 / A1 / 21-87 / - / - / 9661.7 / 4.51 / 5.7 / 10.3 / 37.09 / 60.46 / -0.497
DcHSF 26 / Dck68693 / A3 / 26-96 / - / - / 10874.2 / 5.61 / 7.2 / 8.2 / 53.61 / 59.69 / -0.427
DcHSF 27 / Dck21515 / A3 / 26-88 / - / - / 9753.0 / 6.53 / 6.8 / 6.8 / 55.35 / 60.91 / -0.424
DcHSF 28 / Dck67381 / B4 / 21-83 / - / (3)LMLDECESILL / 9621.8 / 4.67 / 7.2 / 14.4 / 56.41 / 82.17 / -0.252
DcHSF 29 / Dck64779 / A4 / 10-112 / - / (127)ISSIYLDL / 18892.7 / 4.66 / 5.8 / 12.7 / 65.25 / 67.63 / -0.461
DcHSF 30 / Dck00765 / B3 / 19-92 / - / (86)LCMSSF / 10638.1 / 4.84 / 8.6 / 12.9 / 66.88 / 69.14 / -0.158
DcHSF 31 / Dck13338 / A7 / 38-100 / - / - / 11225.6 / 5.06 / 7 / 11 / 39.80 / 78.90 / -0.297
DcHSF 32 / Dck25002 / A2 / 54-116 / - / - / 12971.6 / 4.57 / 8.6 / 17.2 / 30.42 / 87.41 / -0.175
DcHSF 33 / Dck57529 / A9 / 6-80 / 200-KKQKTLKSSGVNKAIKRVGRL-220 / (7)LTIFIL / 44635.5 / 9.49 / 12.4 / 8.6 / 39.13 / 74.87 / -0.450
DcHSF 34 / Dck21253 / A9 / 61-151 / 300-RKKPRAMKPRHQKLTRRRRP-319 / - / 43941.4 / 9.18 / 16.9 / 14.1 / 49.88 / 70.67 / -0.753
DcHSF 35 / Dck19523 / A1 / 21-86 / 194 -GATKKRRQPK-203 / - / 48915.5 / 5.24 / 9.6 / 13.6 / 48.62 / 71.42 / -0.732