Supplementary Figure S3. Alignment of Conserved Region Shared by the Pth11-Related Proteins

Supplementary Figure S3. Alignment of conserved region shared by the Pth11-related proteins containing the CFEM domain.

MG05871.4 : ------IVRILSRLYS------SGRLWWDDYTCLVASVFLFG-MLGMQLES—ARLGFGKHIWVIDEMPGLSLLK
MG01867.4 : ------LLRLLSKVEFPRNTVRGGWLPTMPEAGWDDLCLLLAWFCTI-VMGIIIGVPLQ-RAALGRDLWTLSKQQLIDTGY
MG07565.4 : ------SARMATKI------AGISTWWWDDTTIVIVFIIH----MATSLAGML-WGMTGTDIWALSPAQINRFLF
MG10438.4 : ------ILRLALKL------AKVSSWYHDDTTIILAW------
MG06755.4 : ------ILRMFSKVFVVG------GSAGLDDWFIVATILLGV-ADTVLIVEGLT-AYGLGRDIWTLTSEQITQFGL
MG07553.4 : ------GTRLATRLFIAPN----G--TGWSLLSSDDWCIVATLAMGI-PSTIVIATGLIPQGHLGMDIWMLEPAAIENFGR
MG10473.4 : ------ILRMSARFMLMNQ----G--GAAVAVGEDDWFILAALVVGV-PNTVIIVSGLVDSGGLGLDIWRLDPETINSFGK
NCU06531.1 : ------LLRLFFRLLTHHPSSGGG--GGRHRLGLDDWVIFLSIPLGI-PYTVLIAHSLA-RAGIGRDVWTLTPQEATWFLK
MG07946.4 : ------AIRVLSKAFV------MGWNQLGLDDLCITIVIVTGIIPATIVTLNGTI-PNGLGVDIWAVPVDHIYQFLK
MG09022.4 : ------VARLVYKVVT------FGWLELGLDDLFISLTIITGVIPSAIITTHGAL-PNGLGVDIWAVPVDNIYTFLK
AN5639.2 : ------ALRLTSRALD------KRLGWDDLLIFIALLFAAS---ISGIGLKLKDTGLGKDIWTVPFEDIRRTLK
AN9444.2 : ------GLRIGWRT------GTKKTVGWDDGFILLAMPDG------AVKYHGLGTNLWTIPFDSITAQLK
MG09455.4 : LVRFGGRLATAMRSNSMAASSANT------RGFRGLWWDDALLFITFIFMVG-IMAMSEVA—LRHGFGDGPWVSGPSDLNLQGL
MG10050.4 : ------CMCIAARLF------GGQGGIGWDDACVTGAICCLVI--TVSGTQSA-VTHGLGRDAFVKSPYDLNISTR
MG09863.4 : ------ALRLASRRLA------REFFIDDVLIYLATLTSLA---LLVPHLIAIQNGFGKHYYDLPDGALTRIVE
MG05871.4 : --YFWFGQMMYIVVQVFGKISILILYIRLFTTP------WFQM------FWKLSMVFMALHGVGYMVLVIVQ
MG01867.4 : --WIYVAEPIYMSSHAFLKASFLFFYLRVFGLVGS--VSYFFGLC------PMRP------LLLGTLWVNLVIGIIIVGLSIGQ
MG07565.4 : --ISFIYGVRYFVNMALIKASILFGLLRVFPNKN------FRR------VLWGTQIFNLFFTLAMLGAWFGQ
MG10438.4 : --VIFIFEPFYFVMICLVKSSILFTYMRIFREPD------AQR------VFRFTHYFNMFQTGVFIVLCFVQ
MG06755.4 : --HMYVIQIMYIARLSLLKMSLLFFYLRIFPNP------RIRQ------LLWGTVAFNTGYFVAFSVTAACL
MG07553.4 : --YFYTVEVLYFAELMLLKMSLLFFYLRIFPSR------GVRR------ILWATIIFNGVYGVAFVLVGIFQ
MG10473.4 : --YFYATAIMYFAEVTLLKMSLLWFYLRIFPSR------GVRL------ALWATIAFNIAFGITFFLLGVLQ
NCU06531.1 : --VFYVLIEYYVAIMAYVKMPFLFMYLRIFGDESK------RTRR------VLWGTVG—TVVLVGGVFLLPVG
MG07946.4 : --FHFIVTIIFFFQVGMLKLALLFFLIRIFLDR------TVKR------LLWATVAVVSTFTTITTLTMALQ
MG09022.4 : --FHFVVSIIYFLQVGLLKMSLLFFYMRIFPAK------EIQR------VLWGTVAFNAAITINFVLMNALQ
AN5639.2 : --LFFIEEELYCICIALVKCSMLMLYLRLFPNR------GLRI------AVFVTLTCTLLWGVGAFFVLLFS
AN9444.2 : NQYLLIAEVFYMPAEAFTQLSFLAFYQRIFPPK------YKY------PIYTLSAISVCFGISNTLIMIFQ
MG09455.4 : --LVFIGEPIYFATSAALKASFLFLFARIFAVGSSGTLQQRWGLPLGGPSSPLRDRWTLAGVLLWAHI-VNVVVAVTFTLVVCFQ
MG10050.4 : --WFFIRSIVFFWTAAACKASLLFQFSRIFNSDG---IDPLWRLPFRGPAWSFKM------VLLCTHMVNLVTCVSFTIFALAQ
MG09863.4 : --PIYILENFYIVTLTFIRISILCLYLRLFYRFG------WFRA------LIIGIILIVVVANGTVLVLTILS
MG05871.4 : CLP-VAAVY---DRS--I-EGKC-MEFNPIVY------SGAALSVFEDVVLVVIPIPELWSLRLN------FKKKMGLMLM
MG01867.4 : CTP-ISYSWTQWAED--S-PGSC-IQSGQLLCGFLGFRAQAISSMLLDFWILALPLSQIARLHRS------LPTKLGVAAM
MG07565.4 : CQP-MSFYWLGWDGE--H-EGSC-VDKKAVAL------AHAGIHLSLDVWILVLPINQIAALNLE------KKKKVGAVLM
MG10438.4 : CRP-LDHFWLGWTGD-DH-PGSC-WHMNDIPV------IHAIVGVLLDVWMFGLPATLVWRLNLK------SREKIGVMLM
MG06755.4 : CRP-IHFFWERWDGE--HLDGIC-MDANAIGW------ANAAVSIALDLWMLALPLSQLGYLKLH------WKKKVGVIIM
MG07553.4 : CNP-ISYFWTKWDGE--H-QGKC-INVNAMAW------SNAAISIALDLWMLAVPMWQLRTLRMH------WKKKIGVGAM
MG10473.4 : CQP-ISYFWTKWDGE--H-EGTC-VDINTVAW------SNAGISIALDIWMLAVPLSQLQALRLN------WKKKVGVALM
NCU06531.1 : CKPSVKVFWEGWDGE--H-EGRC-DNINASAW------TLSIVTIVLDLWIMAIPLSQLRKLNMD------WKKKLAVGLM
MG07946.4 : CYPRTEAFWMSWDGSPEY-QGECKIDVTALSL------SNASFSVIADIWMLAIPLWKLRKLQLQ------KKKKLSVAAM
MG09022.4 : CAPNPQLFWTAWDGEPQH-QGSCNLSFTDVSL------GSAAFSIAIDVWMLAVPMWELRKLQLH------WTKKLGVAIM
AN5639.2 : CRP-ISHYWNSWDGE--H-KGSC-LSHNDILL------AHSTINIILDVAITIIPMPIVLKLHMP------VGKRLAVLFM
AN9444.2 : CTP-VSFFWNSWTGE--V-SGTC-ININTYSW------YRAAMQICMDVSIISLPIWPLSRLALS------TRKKAMVLVM
MG09455.4 : CQP-LDYFWKGWDGI--T-PGTC-INRKAVPL------AHGVVSIMLDIWILIIPLCLLPALRVS------FWTKIGIASM
MG10050.4 : CAP-MSYYWTQWNSP--Q-NGSCIVNKRLPSF------GHSIVGVLLDFWILALPLRLISFLNIS------KGTKVAAASM
MG09863.4 : CRP-TERIWDT-PGP--R-DATC-MDVHSLST------SVATLSTAEDFLLVLLPLMPMLVDRLNKPPMPGFLVPLRSRVYVVGM
MG05871.4 : FAIGLV------ATVTSIVRINYLV-KIGFT-YDQPWDNVDPITWSVIEEFCAIICGSLPSC : 127-359

MG01867.4 : FSLGII------ATGVSIVRARILD-TTASS-DNPGMMMSNSATWSMVESSVGIVCACLPGM : 144-408

MG07565.4 : FGVGIF------LTIVCALRINALV-DGTRP-RSISTRGFWTALWSNIENSVGIMVACMPAM : 122-355

MG10438.4 : FGFGVF------LIVISCIRIPLLL-NVSPA-TAVTDESLQGAIWSSVEVCIGICVACMPAL : 124-325

MG06755.4 : FAVGTF------VTVMSVLRLQSLV-SFANS-DNPTWDNWDVVRWSTIEVNVGVICACLPPL : 177-355

MG07553.4 : FVVGTL------ITVVSVLRLQSLV-DFASS-DNPTQTSWDVTFWSLLEIKVGIFCACMPTL : 109-325

MG10473.4 : FCVGTL------VTVVSVLRLQSLV-AFARS-DNPTMDSWDVTSWSVIEINVGIMCTCLPSL : 120-363

NCU06531.1 : LCVGVF------DTMISIIRLHSLL-AFQPS-TNVTWDYYPVAVWSAVEYHVAVICACLPAM : 141-388

MG07946.4 : FAMGTF------STIISCYRIHILS-HGRNSHDNFTYTELPISLWSVVELMVGVICACMPAM : 115-359

MG09022.4 : FATGTF------FTVLSCIRLRILA-DVTRTLENVTYDQLEISIWSCVEITVGIICACMPTM : 121-365

AN5639.2 : FGVGLA------VTIISIMRLVETV-GFNST-QNPTKDFVPVGIWSLLEFDVAILCACMPAM : 91-327

AN9444.2 : FCTGFLSNNKTRITIVSCLRLQSLI-KFSKS-PNTSMDNNPAIYWSMVECDVAIICACMPSL : 117-352

MG09455.4 : FCVGTL------TTVVSAIRCVPVSRDINSS-YSLTWNQAEVLNWTQIELVTGIVCACMPGH : 234-510

MG10050.4 : YCLGAL------VTVISIVRVSILMRDLNIS-SDLLSNYIAVTKWSQAEMATAIVCACIIPA : 123-376

MG09863.4 : FAVGTL------AATASAYRLDTLL-QIGTS-SDPSWDYVPVAHWSLLEFTGGIVVACLPAI : 122-366

Alignment of the conserved pth11-domain in CFEM-GPCRs was done using T-Coffee. The accession numbers of the predicted proteins in the M. grisea, N. crassa, and A. nidulans genome database are indicted on the left. The boundaries of each sequence used in the alignment are indicated on the right.