Supplementary figure
Figure 1 Expression profile of lincRNA transcripts in GCA. A. Hierarchical clustering map. LincRNA transcripts of tumor tissues (T) and adjacent normal tissues (N) of five patients with GCA were detected. In the heatmap, columns represent samples, and rows represent each gene. Red and green colors indicate high and low expression, respectively. The scale of expression level is shown on the horizontal bar. B. Scatter plot of differentially expressed lincRNAs in tumor tissues and adjacent normal tissues. Red and green colors indicate high and low expression, respectively.
Supplementary tables
Table 1 Clinicopathologic characteristics of gastric cardia adenocarcinoma cases
Groups / N (%)Age
＜50 / 24(19.8)
≥50 / 97(80.2)
Gender
Male / 96(79.3)
Female / 25(20.7)
TNM stage
Ⅰ / 3(2.5)
Ⅱ / 51(42.2)
Ⅲ / 58(47.9)
Ⅳ / 9(7.4)
Pathological differentiation of tumor
Well / 39(32.2)
Moderate / 46(38.0)
Poor / 36(29.8)
Depth of invasion
T1/2 / 38(31.4)
T3/4 / 83(68.6)
LN metastasis
negative (N0) / 19(15.7)
positive (N1/2/3) / 102(84.3)
Distant metastasis or recurrence
negative / 56(46.3)
positive / 65(53.7)
Family history of UGIC
negative / 70(57.9)
positive / 51(42.1)
Vital statistics
Alive / 31(25.6)
Dead GCA / 70(57.8)
Dead unrelated / 6(5.0)
Information unavailable / 14(11.6)
Table 2 Primer sequences and reaction conditions of LOC100130476 used in this study
PCR types / Gene / Primers / Annealing temperature (℃) / Product size (bp)RT-PCR / LOC100130476 / F:5’-AGACAGTCTAACCGTGGGGTA-3’
R:5’-CCCAAATTGTGCAGGACCAG-3’ / 58 / 238
GAPDH / F:5’-AGGTGAAGGTCGGAGTCAACG-3’
R:5’-AGGGGTCATTGATGGCAACA-3’ / 104
BGS / CpG island 1 / F:5’-TTGGGAAGTTGTTTTTGGGAT-3’
R:5’-CAACTATTCCTTTCAAATAATAC-3’ / 56 / 275
CpG island 2 / F:5’-TTAATAAATTTTTAGTTTAGG-3’
R:5’-TAACTTTAAAAAATCCC-3’ / 55 / 500
CpG island 3 / F:5’-TGGGAATTGGAAATGAAATAG-3’
R:5’-ATCCAAAACTAACAAAAACAAT-3’ / 55 / 278
BS-MSP / Region 1
First-step / F:5’-TTGGGAAGTTGTTTTTGGGAT-3’
R:5’-CAACTATTCCTTTCAAATAATAC-3’ / 56 / 275
Second-step
Methylation / F:5’-TATTGCGGTATTTTACGTTTCGTCG-3’
R:5’-AAACGCCCCTCGAACCCTCCGATCG-3’ / 54 / 169
Unmethylation / F:5’-TATTGTGGTATTTTATGTTTTGTTG-3’
R:5’-AAACACCCCTCAAACCCTCCAATCA-3’ / 50 / 169
Region 2
First-step / F:5’-TTAATAAATTTTTAGTTTAGG-3’
R:5’-TAACTTTAAAAAATCCC-3’ / 55 / 500
Second-step
Methylation / F:5’-CGGTGAGTTCGAGGTAGACGGTACG-3’
F:5’-CGCCCGATCCCTACCCCGACCCCCG-3’ / 53 / 182
Unmethylation / F:5’-TGGTGAGTTTGAGGTAGATGGTATG-3’
F:5’-CACCCAATCCCTACCCCAACCCCCA-3’ / 54 / 182
Region 3
First-step / F:5’-TGGGAATTGGAAATGAAATAG-3’
R:5’-ATCCAAAACTAACAAAAACAAT-3’ / 55 / 278
Second-step
Methylation / F:5’-AAAATTATTTTGGCGTTCGGGTTCG-3’
R:5’-CCGCTACTTTTTCTCGAAATACCCG-3’ / 52 / 129
Unmethylation / F:5’-AAAATTATTTTGGTGTTTGGGTTTG-3’
R:5’-CCACTACTTTTTCTCAAAATACCCA-3’ / 54 / 129
F: Forward primer; R: Reverse primer.