Supplementary Figure 2, Ingenuity Pathways Analysis.

Ingenuity Pathways Analysis of the effects of Pel. Shaded symbols indicate proteins identified from the input list. Red symbols are proteins that increased in abundance; green symbols are proteins that decreased. The color intensity indicates the degree of change. Grey symbols are proteins that were identified but did not change significantly in the DIGE analysis. Unfilled symbols are molecules in the Ingenuity network that were not identified in our study. Symbols such as for ATPase that show grey and red regions reflect protein families that showed differential responses of various isoforms. An asterix identifies multiple entries in the dataset that map to a single protein in the network database. In Ingenuity functions are represented by the shape of symbols: enzymes (diamonds), transcription regulators (oval), groups/complexes (double circle), kinases (triangle down), phosphatases (triangle up), transporters (trapezoids), cytokines (squares) and others (circles). An arrow indicates a direct action, a line indicates binding and a dotted line represents an indirect interaction.

Network 1.Ingenuity Pathway “Hematological disease, immunological disease, respiratory disease”Score 40.


Network 2. Ingenuity Pathway “Post-translational modification, protein folding, cancer” Score 35.


Network 3.Ingenuity Pathway “Protein Synthesis, cell death, respiratory disease”Score 33.


Abbreviations:ACAT2 acetyl-Coenzyme A acetyltransferase 2; ACTB actin, beta; Actin ; ANXA5 annexin A5; ANXA6 annexin A6; ATPase ATP synthase; ATP5B ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP5J ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F; ATP8 ATP synthase subunit 8; BAG2 BCL2-associated athanogene 2; BIK BCL2-interacting killer (apoptosis-inducing); CCT2 CTP:phosphocholine cytidylyltransferase 2; CCT3 chaperonin containing TCP1, subunit 3 (gamma); CCT5 chaperonin containing TCP1, subunit 5 (epsilon); Ck2 Casein kinase II alpha subunit; CLIC4 chloride intracellular channel 4; COTL1 coactosin-like 1 (Dictyostelium); CRYAB crystallin, alpha B; CSDE1 cold shock domain containing E1, RNA binding; DDX1 Dead-box-1; DSTN destrin; E2f E2F transcription factor; EEF1D eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); EIF3D eukaryotic translation initiation factor 3, subunit D; EIF3F eukaryotic translation initiation factor 3, subunit F; EIF4B eukaryotic translation initiation factor 4B; EIF4E eukaryotic translation initiation factor 4E; ERK Extracellular regulated MAP kinase; ERN1 (includes EG:2081); F Actin ; FUBP1 far upstream element (FUSE) binding protein 1; GARS glycyl-tRNA synthetase; Histone h3; HNRNPA1 heterogeneous nuclear ribonucleoprotein A1; HNRNPC heterogeneous nuclear ribonucleoprotein C; HNRNPK heterogeneous nuclear ribonucleoprotein K; Hsp70; Hsp90 ; HSP90AA2 heat shock protein 90kDa alpha (cytosolic), class A member 2; HSP90B1 heat shock protein 90kDa beta (Grp94), member 1; HSPA1B heat shock protein 1B; HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSPH1 heat shock 105kDa/110kDa protein 1; hydrogen peroxide ; IFI6 interferon, alpha-inducible protein 6; IGK@ immunoglobulin kappa locus; IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; Immunoproteasome Pa28/20s proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Insulin; KIF1C kinesin family member 1C; LMNB2 lamin B2; MCM7 minichromosome maintenance complex component 7; MSR1 macrophage scavenger receptor 1; MYC v-myc myelocytomatosis viral oncogene homolog (avian); MYH9 myosin, heavy chain 9, non-muscle; NCALD neurocalcin delta; PCBP2 poly(rC) binding protein 2; PCNA proliferating cell nuclear antigen; PDGFB platelet derived growth factor, B polypeptide; PFDN4 prefoldin 4; PHACTR1 phosphatase and actin regulator 1; PRDX1 peroxiredoxin 1; PRDX3 peroxiredoxin 3; Proteasome PA700/20s proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); PSMA1 proteasome (prosome, macropain) subunit, alpha type, 1; PSMB4 proteasome (prosome, macropain) subunit, beta type, 4; PSMC4 proteasome (prosome, macropain) 26S subunit; PSME3 proteaseome (prosome, macropain) 28 subunit, 3; RBM41 RNA binding motif protein 41; RNA polymerase II RNA polymerase II protein; RPS3 ribosomal protein S3; RPSA ribosomal protein SA (laminin receptor 1); RUVBL1 RuvB-like 1 (E. coli); SDHA succinate dehydrogenase complex, subunit A, flavoprotein (Fp); SET SET translocation (myeloid leukemia-associated),; SLN (includes EG:6588); SRXN1 sulfiredoxin 1 homolog (S. cerevisiae); STIP1 stress-induced phosphoprotein 1; STMN1 stathmin 1/oncoprotein 18; TCP1 t-complex protein 1; TMPO thymopoietin; TNF tumor necrosis factor (TNF superfamily, member 2); TRAF6 TNF receptor-associated factor 6; TRIM28 tripartite motif-containing 28; TRPM7 transient receptor potential cation channel, subfamily M, member 7; TUBA1C tubulin, alpha 1c; TUBB tubulin, beta; TUBB1 tubulin, beta 1; Tubulin beta-Tubulin at 56D; VCP valosin containing protein; XPO6 exportin 6.

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