Supplementary Figure 1. Segregation of the NAU2277 marker in the BC2S1 population.

M: DNA marker; P1:H7124; P2:0768; F1:(H7124xTM-1); 1-43:individuals from BC2S1 population

Supplementary Table 1. The phenotypic mean values for fiber qualities and yield parameters between the recipient parent and the CSILs across three environments.

CSIL/
parent / Traits / IL040-A4-1 / Dif.fa / IL080-D6-1 / Diff / IL088-A7-3 / Diff / IL019-A2-6 / Diff / TM-1 / Hai7124 /
2009 / FS
(cN/tex) / 31.0±0.52 / 1.8** / 31.5±0.53 / 2.3** / 31.0±0.36 / 1.8** / 30.1±0.37 / 0.9* / 29.2±0.39 / 35.2±0.43 /
/ FL
(mm) / 30.5±0.65 / 1.7** / 30.8±0.42 / 2.1** / 29.7±0.92 / 0.9** / 30.0±0.91 / 1.2** / 28.8±0.47 / 33.9±0.51 /
/ FM / 4.5±0.12 / -0.6** / 4.5±0.04 / -0.6** / 4.8±0.06 / -0.3* / 4.7±0.13 / -0.4** / 5.1±0.07 / 4.6±0.14 /
/ NB / 13.2±0.55 / 2.0** / 12.3±0.78 / 1.1* / 13.4±1.44 / 2.2** / 12.8±1.28 / 1.6** / 11.2±0.82 / 20.2±0.65 /
/ BW
(g) / 6.9±0.23 / -0.1 / 7.0±0.72 / 0.0 / 6.6±0.48 / -0.4* / 7.2±0.58 / -0.2 / 7.0±0.77 / 3.5±0.34 /
/ LP
(%) / 30.7±1.45 / -2.2** / 31.5±1.21 / -1.4* / 32.1±1.69 / -0.8 / 31.7±0.82 / -1.2* / 32.9±0.93 / 33.2±1.43 /
2010 / FS
(cN/tex) / 31.7±0.35 / 1.8** / 30.5±0.28 / 0.6* / 30.9±0.53 / 1.0** / 30.7±0.47 / 0.8* / 29.9±0.76 / 36.2±0.43 /
/ FL
(mm) / 30.1±0.15 / -0.5* / 32.4±0.36 / 1.8** / 32.1±0.36 / 1.5** / 31.3±0.99 / 0.7* / 30.6±0.37 / 33.6±0.57 /
/ FM / 4.6±0.20 / -0.6** / 4.5±0.18 / -0.7** / 5.2±0.49 / 0.0 / 4.5±0.08 / -0.7** / 5.2±0.02 / 4.4±0.05 /
/ NB / 11.5±1.62 / 1.3** / 9.3±1.91 / -0.9* / 10.3±1.31 / 0.1 / 12.1±1.68 / 1.9** / 10.2±0.95 / 18.1±1.76 /
/ BW
(g) / 6.4±0.37 / 0.2** / 6.5±0.23 / 0.3** / 6.3±0.29 / 0.1 / 6.1±0.38 / -0.1 / 6.2±0.22 / 3.2±0.15 /
/ LP
(%) / 32.8±0.83 / 0.7 / 31.3±0.67 / -0.8 / 31.5±1.24 / 0.4 / 30.8±1.55 / -1.3** / 32.1±1.61 / 34.2±1.25 /

Field trial at Aksu in 2009 and 2010. *, ** , Significance from TM-1 at 0.05 and 0.01 levels, respectively. a the difference between each introgressed homozygous line for G. barbadense allele and TM-1.

Supplementary Table 2. The fiber quality and yield component heritability (h2B ) in the CSILs.

Traits / h2B (%)
FS / 0.78
FL / 0.86
FM / 0.87
NB / 0.31
BW / 0.72
LP(%) / 0.91


Supplementary Table 3. Analysis of variance for the four or five-environment field trials (E1~E5)

Lines / Traits / Source / Degree of
freedom / Sum of squares / Mean squares / F value / p
3326-3 / FL / Genotype(A) / 1 / 23.26 / 23.26 / 87.16** / 0.00
Environment(B) / 4 / 10.24 / 2.56 / 19.10 / 0.66
A×B / 4 / 1.06 / 0.27 / 0.48 / 0.75
FS / Genotype(A) / 1 / 5.51 / 5.51 / 10.05* / 0.03
Environment(B) / 4 / 20.42 / 5.11 / 7.24* / 0.02
A×B / 4 / 2.19 / 0.55 / 3.79 / 0.08
FM / Genotype(A) / 1 / 1.81 / 1.82 / 15.35* / 0.01
Environment(B) / 4 / 4.90 / 1.23 / 9.26* / 0.02
A×B / 4 / 0.51 / 0.13 / 6.26* / 0.03
3380-4 / FL / Genotype(A) / 1 / 4.36 / 4.36 / 50.28** / 0.00
Environment(B) / 4 / 5.75 / 1.43 / 0.89 / 0.53
A×B / 4 / 0.34 / 0.08 / 2.12 / 0.21
FS / Genotype(A) / 1 / 1.90 / 1.90 / 19.76* / 0.02
Environment(B) / 4 / 28.2 / 7.06 / 30.77** / 0.00
A×B / 4 / 0.38 / 0.09 / 0.76 / 0.59
FM / Genotype(A) / 1 / 0.80 / 0.80 / 17.62* / 0.02
Environment(B) / 4 / 1.89 / 0.47 / 9.65* / 0.03
A×B / 4 / 0.18 / 0.04 / 3.36 / 0.10
3426-5 / FL / Genotype(A) / 1 / 7.22 / 7.22 / 201.62** / 0.00
Environment(B) / 3 / 10.63 / 3.54 / 4.89 / 0.13
A×B / 3 / 0.11 / 0.035 / 0.18 / 0.90
FS / Genotype(A) / 1 / 9.42 / 9.42 / 71.32** / 0.00
Environment(B) / 3 / 37.71 / 12.57 / 7.488* / 0.04
A×B / 3 / 0.39 / 0.13 / 0.88 / 0.52
FM / Genotype(A) / 1 / 1.04 / 1.04 / 17.87* / 0.02
Environment(B) / 3 / 0.73 / 0.24 / 11.15 / 0.53
A×B / 3 / 0.17 / 0.058 / 1.33 / 0.38
3389-2 / FL / Genotype(A) / 1 / 13.30 / 13.30 / 19.67* / 0.01
Environment(B) / 4 / 53.71 / 13.42 / 5.56 / 0.06
A×B / 4 / 5.50 / 1.375 / 1.95 / 0.24
FS / Genotype(A) / 1 / 7.03 / 7.032 / 22.04** / 0.00
Environment(B) / 4 / 16.58 / 4.14 / 17.01 / 0.12
A×B / 4 / 1.27 / 0.31 / 1.24 / 0.40
FM / Genotype(A) / 1 / 1.13 / 1.13 / 14.05* / 0.02
Environment(B) / 4 / 1.19 / 0.29 / 1.21 / 0.40
A×B / 4 / 0.32 / 0.08 / 1.57 / 0.31
3262-4 / FL / Genotype(A) / 1 / 20.62 / 20.62 / 12.43* / 0.02
Environment(B) / 4 / 88.04 / 22.01 / 9.79** / 0.00
A×B / 4 / 6.63 / 1.65 / 5.43 / 0.06
FS / Genotype(A) / 1 / 5.09 / 5.09 / 15.54** / 0.00
Environment(B) / 4 / 37.17 / 9.29 / 5.91 / 0.06
A×B / 4 / 3.67 / 0.91 / 5.97 / 0.07
FM / Genotype(A) / 1 / 0.22 / 0.22 / 12.91* / 0.03
Environment(B) / 4 / 2.03 / 0.50 / 9.42 / 0.20
A×B / 4 / 0.30 / 0.07 / 1.26 / 0.39

Plants were grown in fields by a randomized complete block design with two replications.* and **, significant at 0.05 and 0.01 level, respectively.