Supplementary data S3 Ranking of apple candidate reference genes in order of their expression stability (M value) calculated by geNorm, and respective pairwise variation values (V).
All samples(buds, flowres, leaves and fruits) / Buds,
flowers and leaves / Buds
(dormant and at initial bursting) / Leaves
(young and mature) / All fruit samples
(development stages, pulp and skin) / Fruit development
(fruit pulp from unripe and mature fruits) / Fruit development
(fruit skin from unripe and mature fruits) / Fruit tissues
(fruit pulp and skin from unripe fruits) / Fruit tissues
(fruit pulp and skin from mature fruits)
Ranking / M / Ranking / M / Ranking / M / Ranking / M / Ranking / M / Ranking / M / Ranking / M / Ranking / M / Ranking / M
MDH/THFS / 0.517 / C3HC4/THFS / 0.348 / ARC5/C3HC4 / 0.115 / SAND/THFS / 0.205 / SAND/THFS / 0.596 / DLD/TMp1 / 0.118 / ACT2/CKL / 0.198 / ARC5/SAND / 0.080 / DLD/TMp1 / 0.139
WD40 / 0.678 / ARC5 / 0.382 / WD40 / 0.172 / ACT11 / 0.247 / MDH / 0.682 / ACT2 / 0.173 / TMp1 / 0.242 / PCS / 0.207 / ACT2 / 0.207
SAND / 0.719 / MDH / 0.409 / MDH / 0.187 / PP2-A1 / 0.260 / WD40 / 0.707 / SAND / 0.225 / ACTfam / 0.274 / GAPDH / 0.249 / ACTfam / 0.279
C3HC4 / 0.752 / TMp1 / 0.451 / THFS / 0.200 / TMp1 / 0.271 / C3HC4 / 0.748 / CKL / 0.254 / DLD / 0.311 / TMp1 / 0.271 / PCS / 0.319
TMp1 / 0.784 / PCS / 0.484 / TMp1 / 0.291 / ARC5 / 0.294 / UBC10 / 0.797 / GAPDH / 0.290 / SAND / 0.333 / CKL / 0.296 / CKL / 0.371
UBC10 / 0.849 / CKL / 0.515 / PCS / 0.403 / MDH / 0.311 / ACT11 / 0.837 / THFS / 0.331 / THFS / 0.375 / THFS / 0.311 / ARC5 / 0.421
ARC5 / 0.887 / PP2-A1 / 0.539 / CKL / 0.469 / CKL / 0.333 / TMp1 / 0.866 / ACTfam / 0.367 / ACT11 / 0.465 / DLD / 0.343 / SAND / 0.461
ACT11 / 0.916 / SAND / 0.568 / ACTfam / 0.515 / PCS / 0.348 / ARC5 / 0.898 / ACT11 / 0.419 / C3HC4 / 0.540 / ACT11 / 0.360 / C3HC4 / 0.493
PP2-A1 / 0.939 / ACTfam / 0.591 / GAPDH / 0.555 / ACT2 / 0.362 / CKL / 0.930 / ARC5 / 0.474 / UBC10 / 0.592 / C3HC4 / 0.378 / ACT11 / 0.531
CKL / 0.916 / ACT2 / 0.622 / SAND / 0.578 / WD40 / 0.376 / PP2-A1 / 0.972 / WD40 / 0.537 / MDH / 0.637 / ACT2 / 0.398 / THFS / 0.581
ACTfam / 1.025 / ACT11 / 0.651 / PP2-A1 / 0.605 / ACTfam / 0.397 / ACTfam / 1.008 / MDH / 0.589 / ARC5 / 0.683 / ACTfam / 0.413 / WD40 / 0.652
PCS / 1.085 / WD40 / 0.678 / ACT2 / 0.651 / TUBa5 / 0.418 / ACT2 / 1.047 / UBC10 / 0.637 / WD40 / 0.731 / MDH / 0.445 / UBC10 / 0.712
ACT2 / 1.145 / UBC10 / 0.727 / ACT11 / 0.690 / C3HC4 / 0.443 / DLD / 1.098 / PP2-A1 / 0.681 / GAPDH / 0.794 / UBC10 / 0.470 / PP2-A1 / 0.777
DLD / 1.191 / GAPDH / 0.771 / UBC10 / 0.749 / GAPDH / 0.470 / PCS / 1.163 / C3HC4 / 0.719 / PP2-A1 / 0.859 / WD40 / 0.507 / GAPDH / 0.840
GAPDH / 1.252 / DLD / 0.851 / DLD / 0.830 / UBC10 / 0.526 / GAPDH / 1.249 / PCS / 0.812 / PCS / 0.995 / PP2-A1 / 0.542 / MDH / 0.903
TUBa5 / 1.617 / TUBa5 / 1.005 / TUBa5 / 1.112 / DLD / 0.571 / TUBa5 / 1.645 / TUBa5 / 1.114 / TUBa5 / 1.272 / TUBa5 / 0.774 / TUBa5 / 1.012
V2/3 / 0.239 / V2/3 / 0.121 / V2/3 / 0.064 / V2/3 / 0.083 / V2/3 / 0.221 / V2/3 / 0.064 / V2/3 / 0.083 / V2/3 / 0.090 / V2/3 / 0.077
V3/4 / 0.162 / V3/4 / 0.154
V4/5 / 0.134 / V4/5 / 0.142
Note: pairwise variation values (V) are presented only to reach the cut-off 0.15. below which it is not necessary the addition of one more gene as reference to a reliable normalization. V2/3 < 0.15 means that the two most stable genes are sufficient to normalize the expression of test genes in each sample set.