Supplemental Information

SupplementalTable 1.Apparent kinetic parameters of CATC with H2O2.

Apparent
kinetic parameters / CATC
Km / 43.12±9.07 mM
Vm / 251.38±44.62 mmolH2O2min-1 mg-1 protein
Kcat / 28.01±4.95×105 S-1

Values are means ±SD of five replicates(n = 5).

SupplementalTable 2.Sequence of primers for CAT genes used for qRT-PCR.

Gene / Primers
CATA / F:5′-CGTCAACACCTACACCTTCG-3′
R: 5′- CTCGTCGTCCATCAAGCAG-3′
CATB / F:5′-CCGTCTGGAACAACAACTCC-3′
R: 5′- GGATACGCTCCCTGTCAAAC-3′
CATC / F:5′-TGCCAAGGAGAACAACTTCA-3′
R: 5′- CCAGTAGGAGAGCCAGATGC-3′
Actin1 / F:5′- CTTCATAGGAATGGAAGCTGCG-3′
R: 5′- CACCTTGATCTTCATGCTGCTA-3′

SupplementalTable 3.Similarities of OsCAThomologs at the levels of cDNA and proteins.

Protein
Gene / CATA / CATB / CATC
CATA / 72.2% / 75.4%
CATB / 66.0% / 83.9%
CATC / 77.2% / 72.6%

DNA sequences alignment using Wilbur-Lipman method，protein sequences alignment using Lipman-Pearson method.

Figure Legends

Supplemental Figure 1.Subcellular localization of CATAisoforms.The GFP was fused to the C-terminus ofCATAisoform. Cells are imaged by a confocal microscope at 14-16 h after the transfection, where the CFP-tagged PTS1 was used as the peroxisomal marker.The red signals represent chlorophyll autofluorescence. The result is representative of three independent experiments. Scale bar = 2 μm.

Supplemental Figure2.Identification of the protein bands from in vivo pull down assay.Thetransgenic rice plants with NHisGLO1 expressed were generated, the youngest fully expanded leaves were detached from T1 or T2 transgenic plants at 5-leaf stage, and homogenized on ice. The homogenate was then affinity-purified to gain the potential GLO-interacting proteins. The purified fractions were further separated bySDS-PAGE and silver-stained, the bands specific to the GLOwere identified bymass spectrometry.The identified CATCpeptides werehighlighted in yellow color.

Supplemental Figure 3.Effects of HPMS and SA on the interaction between GLO and CATas detected by BiFC.

BiFCfluorescence of the rice protoplasts treated with HPMS: 500 μM or SA: 100 μM.