Supplementalfig. S1. Deduced Amino Acid Sequence of Osmyb2p-1. Five Predicted Sumoylation

SupplementalFig. S1. Deduced amino acid sequence of OsMYB2P-1. Five predicted sumoylation domains are highlighted in green on the sequence. R2, R3 and DUF3651 domains are highlighted with dot lines on the sequence.

Supplemental Fig. S2. Response of OsMYB2P-1 to -N, and –K in leaves and stems. Expression was normalized to that of Actin. Data are means ±SD (n=3).

Supplemental Fig. S3. Effect of Pi availability in the medium on root architecture in wild-type and transgenic Arabidopsis. (A) Root morphology of wild-type (WT) and transgenic rice under Pi-sufficient (HP, 1mM; top) or Pi-deficient (10 µM; bottom) conditions for 7days. B-C, Quantitative analysis of the lateral roots (B) and the number of length of primary roots (C) of wild-type and transgenic Arabidopsis grown in the Pi-sufficient or Pi-deficient medium for 7 days. Error bars indicate standard deviation.Asterisk indicates significant difference at P0.05 compared with the wild type by Student’s t test.

SupplementalFig. S4.Expression of Pi starvation-induced genes in the WT and OsMYB2P-1 transgenic plants. Total RNA samples were extracted from shoots of seedling grown in normal nutrient solution for 7 days, followed by treatment with HP or LP medium for 14 days. Expression was normalized to that of Actin. Data are means±SD (n=3). Asterisk indicates significant difference at P0.05compared with the wild type by Student’s t test.

SupplementalFig. S5. Expression of Pi transporter genes in the WT and OsMYB2P-1 transgenic plants. Total RNA samples were extracted from shoots of seedling grown in normal nutrient solution for 7 days, followed by treatment withHP or LP medium for 14 days. Expression was normalized to that of Actin. Data are means ±SD (n=3). Asterisk indicates significant difference at P0.05 compared with the wild type by Student’s t test.

SupplementalFig. S6. Fe contents in wild type and OsMYB2P-1 overexpression transgenic rice under Pi-sufficient conditions (A) and Pi-deficient conditions (B).

Supplemental Fig. S7. OsMYB2P-1 expression response to cold, salt, and PEG stress.

Supplemental Fig. S8.Plasmids construction for plant transformation. (A) schematic representation of the RNAi construct of OsMYB2P-1 used for rice transformation. (B) schematic representation of the over-expressed construct of OsMYB2P-1 used for rice transformation.(C) schematic representation of the over-expressed construct of OsMYB2P-1 used for Arabidopsis transformation.

Supplemental Fig. S9. Expression of OsMYB2P-1 in the roots of the transgenic plants. OE-5, OE-11, OE-12, RNAi-1 and RNAi-2 represent independent OsMYB2P-1 overexpressing andRNA interference lines, respectively. Total RNA samples were extracted from roots of seedling grown in normal nutrient solution for 7 days, followed by treatment with HP or LP medium for 14 days. Expression was normalized to that of Actin. Data are means ±SD (n=3). Asterisk indicates significant difference at P0.05 compared with the wild type by Student’s t test.