Supplemental Table S1. 90K iSelect SNP array markers linked to Sr42 in PI595667/LMPG-6 population and their ID, position on reference maps (Wang et al. 2014) and BLAST hit information
ID / SNP name / Chr / Position / Best Blast hitIWA6673 / wsnp_Ku_c19587_29102203 / 6D / 9.47 / NA
IWA2338 / wsnp_Ex_c18664_27540364 / 6D / 9.47 / 6DS_2093399
IWB64081 / RFL_Contig2815_1135 / 6D / 9.47 / 6DS_2073423
IWB21614 / Excalibur_c10358_1800 / 6D / 14.74 / 6DS_2093399
IWB13767 / CAP7_c1208_150 / 6D / 15.14 / 6DS_2121565
IWB47157 / Kukri_c64412_175 / 6D / 16.24 / 6DS_2073423
IWB36391a / IACX9471 / 6D / 18.20 / 6DS_2116396
IWB31561a / Excalibur_s114066_247 / 6D / 20.75 / 6DS_2110694
IWB30767 / Excalibur_rep_c66622_1066 / 6D / 20.75 / 6DS_2110694
IWB6902 / BS00022094_51 / 6D / 23.84 / 6DS_2073344
IWB52065 / Ra_c42576_780 / 6D / 23.84 / 6DS_2116396
IWB15852a / D_contig17056_55 / 6D / 23.84 / 6DS_2076424
a Array SNPs converted to KASP assays
Supplemental Table S2. Primer sequence information for KASP assays
SNP / Primer_Allele.T / Primer_Allele.HEX / Primer_Common / Allele.T / Allele.HEXIWB15852 / GTATGTTTTGATCTGAAGCAAACTAATTCTAT / ATGTTTTGATCTGAAGCAAACTAATTCTAC / GTGTAAGATATGCAGAGGCGATGTTATTT / A / G
IWB31561 / TCTTCCGTGAAGTGCTAATCTGT / CTTCCGTGAAGTGCTAATCTGC / CACAAGTTTGCGAGGACAACAAACAAATA / T / C
IWB36391 / AAGATGATGAACAAGTGGCCCCTT / GATGATGAACAAGTGGCCCCTC / ACGCAAAACCGCATACATCATTGGATTT / T / C
TP43472 / GTACCGCAGCGACGACGCA / ACCGCAGCGACGACGCG / CCCGTCTTCTTCACTCACGGCTT / T / C
TP93838 / GCACGCCGTCGTCTCTGTC / GCACGCCGTCGTCTCTGTA / GCGGCGGCAGCGGAGACAA / G / T
Supplemental Table S3. Parental lines and their SNP haplotypes for three KASP markers near the 6DS-Ug99 locus. Lines are grouped by SNP haplotype.
ChineseSpring / - / T*a / C / T
Norin 40 (Sr42) / Sr42 / T* / C / T
Eagle10 / Sr42 / T* / C / T
Ember / Sr42 / T* / C / T
Guard / Sr42 / T* / C / T
Ripper / Sr42 / T* / C / T
Shield / Sr42 / T* / C / T
AC Cadillac (SrCad) / SrCad / C / C / T
Briggs / - / C / C / T
Peace (SrCad) / SrCad / C / C / T
PI595667 (SrCad) / SrCad / C / C / T
Blouk / SrTmp / T / C / T
CnSSrTmp (SrTmp) / SrTmp / T / C / T
Digalu / SrTmp / T / C / T
Pfunye / SrTmp / T / C / T
PI410954 (SrTmp) / SrTmp / T / C / T
Robin / SrTmp / T / C / T
Triumph64 (SrTmp) / SrTmp / T / C / T
Cakuke / - / C / T / C
BillBrown / - / T* / T / C
Faller / - / T* / T / C
Linkert / - / T* / T / C
LMPG-6 / - / T* / T / C
Norden / - / T* / T / C
RB07 / - / T* / T / C
Rollag / - / T* / T / C
Sabin / - / T* / T / C
Tom / - / T* / T / C
Ulen / - / T* / T / C
a A T* represents KASP results that were not grouped with C or T, but were closer to T. This may represent an imperfect sequence match of the template DNA with either primer.
Figure S1. Manhattan plot of 6D SNPs associated with non-Sr24 resistance in the PI 410954 TC1F1 population