Supplemental Table S1. PCR Primers Used in This Study

Supplemental Table S1. PCR primers used in this study.

Primer name / Sequence
<For Cl-PT1a>
5’UTR-F / GCGGGGGCAAAATAGTAGA
3’UTR-R / CGATTATTGATCGGTGAATGC
ΔTP-Cl-PT1a-F / GTACTAGTATGGGCTTGAACAGAAGTGAAAGCAAGA
ΔTP-Cl-PT1a-R / TCTCGAGTCATCGTAAGAAGTGAATAAGGAGATA
Cl-PT1a-TP-F / GTGGATCCATGCTTCAAATGCATTCGAATTC
Cl-PT1a-TP-R / CACTCGAGGCAACGCGGTATTTGTTGAACG
Cl-PT1a-qPCR-F / GTAAATCAGGTGGCCGATGT
Cl-PT1a-qPCR-R / GGCTAAGTGCGATAGCCAAG
< For Cl-PT1b>
DGP-F1 / GCNHTNTTYAARGAYATHCCNGA
DGP-F2 / ATHCCNGAYRTHGARGGNGAY
DGP-R1 / TTYTAYAKNTTYATHTGGAAVYT
5’RACE-R / GACTAACTGGAAAGACGAAGAAGCTCC
3’RACE-F / CATAGTTGGAGCTTCTTCGTCTTTCC
Cl-PT1b-F1 / GCTCGAGATGCTTCAAATGCATTCGAGTTCAAGC
Cl-PT1b-R1 / TGGATCCTCATCGTAAGAAGTGAATAAGGAGAT
Cl-PT1b-F2 / ATGCTTCAAATGCATTCGAATTCAAGCTTCTCTCC
Cl-PT1b-R2 / TCATCGTAAGAAGTGAATAAGGAGATATTCAGC
Cl-PT1b-qPCR-F / CGTCCCTTCGCTATGATTGG
Cl-PT1b-qPCR-R / TGCAGCGGCAGAATAGCA
<For other genes>
CaMV35Spro-F / CACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAA
26S rRNA-qPCR-F / CCTTCGATAAGCCTGTGGAG
26S rRNA-qPCR-R / CAGACTCCTTGGTCCGTGTT

Restriction sites are underlined. In the degenerate primer sequences of Cl-PT1b (DSP-F1, DGP-F2, and R1), Y = C or T, R = A or G, K = G or T, H = A or C or T, V = C or A or G, and N = A or C or G or T.

Supplemental Table S2. Quantitative analysis of 8GU in peel of various citrus species.

Common name / Swingle's classification system / Tanaka's classification system / 8GU content in peel (mg.kg-1 FW)
Mexican lime / C. aurantifolia (Christm.) Swing. / C. aurantifolia (Christm.) Swing. / N. D.
Brazil sweet lime / C. aurantifolia (Christm.) Swing. / C. limettioides Tan. / N. D.
Macrophylla or Alemow / C. aurantifolia (Christm.) Swing. / C. macrophylla Wester / N. D.
Excelsa / C. aurantifolia (Christm.) Swing. / C. excelsa Wester / N. D.
Palestine sweet lime / C. aurantifolia (Christm.) Swing. / C. limettioides Tan. / N. D.
Coppenrhad lime / C. aurantifolia (Christm.) Swing. / C. aurantifolia (Christm.) Swing. / N. D.
Tahiti lime / C. aurantifolia (Christm.) Swing. / C. latifolia (Yu. Tan.) Tan. / N. D.
Bears lime / C. aurantifolia (Christm.) Swing. / C. latifolia (Yu. Tan.) Tan. / N. D.
Gallet / C. aurantifolia (Christm.) Swing. / C. aurantifolia (Christm.) Swing. / N. D.
Giant Key lime / C. aurantifolia (Christm.) Swing. / C. aurantifolia (Christm.) Swing. / N. D.
Castagnaro Bergamote / C. aurantifolia var Bergamia / C. bergamia Risso and Poit. / N. D.
Maroc sour orange / C. aurantium L. / C. aurantium L. / N. D.
Bouquettier de Nice sour orange / C. aurantium L. / C. aurantium L. / N. D.
Granito Sour orange / C. aurantium L. / C. aurantium L. / N. D.
Chinotto / C. aurantium L. / C. myrtifolia Raf. / N. D.
Mountain / C. halimii B.C. Stone (Tan. Class.) / C. halimii B.C. Stone / N. D.
Combava / C. hystrix D.C. / C. hystrix D.C. / N. D.
Yuzu / C. ichang austera ex. Tan. hybrid / C. junos Siebold / 3,23 ± 0,68
Ichang / C. ichangensis Swing. / C. ichangensis Swing. / N. D.
Khazi / C. latipes (Swing.) Tan. / C. latipes (Swing.) Tan. / N. D.
Marrakech lime / C. limon (L.) Burm. f. / C. limetta Risso / 1,18 ± 1,25
Pipless pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Pink pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Chandler pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Kao Pan pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Reinking pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Tahiti pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Deep Red pummelo / C. maxima (Burm.) Merr. / C. maxima (Burm.) Merr. / N. D.
Corsican citron / C. medica L. / C. medica L. / N. D.
Etrog citron / C. medica L. / C. medica L. / N. D.
Buddha's hand citron / C. medica L. / C. medica L. / N. D.
Star Ruby grapefruit / C. paradisi Macf. / C. paradisi Macf. / N. D.
Duncan grapefruit / C. paradisi Macf. / C. paradisi Macf. / N. D.
Marsh grapefruit / C. paradisi Macf. / C. paradisi Macf. / N. D.
Willow Leaf mandarin / C. reticulata Blanco / C. deliciosa Ten. / N. D.
Fuzhu mandarin / C. reticulata Blanco / C. erythrosa Hort. ex Tan. / N. D.
San Hu Hong Chu mandarin / C. reticulata Blanco / C. erythrosa Hort. ex Tan. / N. D.
Nan Feng Mi Chu mandarin / C. reticulata Blanco / C. kinokuni Hort. ex Tan. / N. D.
Beauty mandarin / C. reticulata Blanco / C. reticulata Blanco / N. D.
Sunki mandarin / C. reticulata Blanco / C. sunki (Hayata) Hort. ex Tan. / N. D.
Dancy mandarin / C. reticulata Blanco / C. tangerina Hort. ex Tan. / N. D.
Bendiguangju / C. reticulata Blanco / C. nobilis Lour. / N. D.
Wase Satsuma / C. reticulata Blanco / C. unshiu Marc. / N. D.
Owari Satsuma / C. reticulata Blanco / C. unshiu Marc. / N. D.
Shekwasha mandarin / C. reticulata Blanco hybrid / C. depressa Hayata / 5,37 ± 3,30
Nasnaran mandarin / C. reticulata Blanco hybrid / C. amblycarpa (Hassk.) Ochse. / 10,82 ± 4,36
Cleopatra mandarin / C. reticulata var austera / C. reshni Hort. ex Tan. / N. D.
Commune Clementine / C. reticulata x C. sinensis / C. clementina Hort.ex Tan. / N. D.
Murcott tangor / C. reticulata x C. sinensis / C. reticulata x C. sinensis / N. D.
Fortune mandarin / (C. reticulata x C sinensis) x C. tangerina / C. clementina x C. tangerina / N. D.
Washington Navel orange / C. sinensis (L.) Osbeck / C. sinensis (L.) Osbeck / N. D.
Shamouti orange / C. sinensis (L.) Osbeck / C. sinensis (L.) Osbeck / N. D.
Hamlin orange / C. sinensis (L.) Osbeck / C. sinensis (L.) Osbeck / N. D.
Pineapple orange / C. sinensis (L.) Osbeck / C. sinensis (L.) Osbeck / N. D.
Orange Pomeroy Trifolite / P. trifoliata (L.) Raph / P. trifoliata (L.) Raph / 6,13 ± 4,97
Orange Flying Dragon Trifoliate / P. trifoliata (L.) Raph / P. trifoliata (L.) Raph / 5,12 ± 5,82
N. D., not detected.