Supplemental Table S1. Origin and source of plasmids.

Plasmids / Characteristics* / Reference
pCW3 / Confers conjugative tetracycline resistance / (46)
pET22b+ / C-terminal His-tag fusion expression vector, 5.5kb, ApR / Novagen
pGEX-KG / N-terminal GST (Glutathione S-transferase) fusion expression vector with thrombin cleavage site and additional linker, 5.0kb, ApR / (17)
pKT25 / N-terminal T25 fusion vector for bacterial two-hybrid system, a derivative of pSU40, 3.4kb / (28)
pMAL-c2X / N-terminal MalE (maltose binding protein) fusion expression vector, has an exact deletion of the malE signal sequence for cytoplasmic expression, 6.7kb, ApR / New England BioLabs
pUT18C / N-terminal T18 fusion vector for bacterial two-hybrid system, a derivative of pUC19, 3.0kb / (28)
pJIR750 / E. coli-C. perfringens shuttle plasmid, CmR / (5)
pJIR2901 / pJIR750  (Asp718/BamHI: 3.0 kb #25544 - #25545 PCR, pCW3) (ptcpH+ complementation vector) / (6)
pJIR2902 / pCW3::tcpHΩ ermQ / (6)
pJIR3075 / pJIR2901  1.9 kb Xmn1 (encodes TcpH1-581) / This study
pJIR3090 / pJIR750  (Asp718/BamHI: 2.0 kb TcpH1-658 #25544 - #28474 PCR, pCW3) / This study
pJIR3091 / pJIR750  (Asp718/BamHI: 2.2 kb TcpH1-753 #25544 - #28473 PCR, pCW3) / This study
pJIR3112 / pJIR750  (Asp718/BamHI: 1.5 kb TcpH1-514 #25544 - #28631 PCR, pCW3) / This study
pJIR3113 / pJIR750  (Asp718/BamHI: 2.0 kb TcpH1-697 #25544 - #28632 PCR, pCW3) / This study
pJIR3115 / pJIR750  (Asp718/BamHI: 2.1 kb TcpH311-450 #25544 - #28693 and #28694 - #25545 SOE PCR, pCW3) / This study
pJIR3116 / pUT18C  (Asp718/BamHI: 2.5 kb TcpH #28691 - #28692 PCR, pCW3) / This study
pJIR3117 / pKT25  (Asp718/BamHI: 2.5 kb TcpH #28691 - #28692 PCR, pCW3) / This study
pJIR3133 / pUT18C  (Asp718/BamHI: 2.0 kb TcpH1-697 #28691 - #28957 PCR, pCW3) / This study
pJIR3134 / pJIR750  (Asp718/BamHI: 2.5 kb TcpH545AAA547 #25544 – #28844 and #28843 - #25545 SOE PCR, pCW3) / This study
pJIR3135 / pJIR750  (Asp718/BamHI: 2.5 kb TcpH242VQAAA246 #25544 - #28847 and #28846 - #25545 SOE PCR, pCW3) / This study
pJIR3139 / pKT25  (Asp718/BamHI: PCR product #28691/#28956 containing TcpH1-514 cloned into pKT25 at BamH1/Kpn1 sites / This study
pJIR3140 / pUT18C  (Asp718/BamHI: PCR product #28691/#28956 containing TcpH1-514 cloned into pUT18C at BamH1/Kpn1 sites / This study
pJIR3141 / pUT18C  (Asp718/BamHI: PCR product #28691/#28958 containing TcpH1-581 cloned into pUT18C at BamH1/Kpn1 sites / This study
pJIR3142 / pKT25  (Asp718/BamHI: 1.7 kb TcpH1-581 #28691 - #28958 PCR, pCW3) / This study
pJIR3143 / pKT25  (Asp718/BamHI: 2.0 kb TcpH1-697 #28691 - #28957 PCR, pCW3) / This study
pJIR3144 / pKT25  (Asp718/BamHI: 2.5 kb TcpF #408272 - #408273 PCR, pCW3) / This study
pJIR3145 / pJIR750  (Asp718/BamHI: 2.5 kb TcpH287DAAAK291 #25544 - #28845 and #28848 - #25545 SOE PCR, pCW3) / This study
pJIR3146 / pKT25  (Asp718/BamHI: 1.0 kb TcpG #29025 - #29024 PCR, pCW3) / This study
pJIR3160 / pKT25  (Asp718/BamHI: 2.5 kb TcpH242VQAAA246 #28691 - #28692 PCR, pJIR3135) / This study
pJIR3161 / pUT18C  (Asp718/BamHI: 2.5 kb PCR TcpH242VQAAA246 #28691 - #28692 PCR, pJIR3135) / This study
pJIR3162 / pKT25  (Asp718/BamHI: 2.5 kb TcpH287DAAAK291 #28691 - #28692 PCR, pJIR3145) / This study
pJIR3163 / pUT18C  (Asp718/BamHI: 2.5 kb TcpH287DAAAK291 #28691 - #28692 PCR, pJIR3145) / This study
pJIR3164 / pKT25  (Asp718/BamHI: 2.5 kb TcpH545AAA547 #28691 - #28692 PCR, pJIR3134) / This study
pJIR3165 / pUT18C  (Asp718/BamHI: 2.5 kb TcpH545AAA547 #28691 - #28692 PCR, pJIR3134) / This study
pJIR3168 / pKT25  (Asp718/BamHI: 1.0 kb TcpB #29119 - #29121 PCR, pCW3) / This study
pJIR3169 / pKT25  (Asp718/BamHI: 0.3 kb TcpD #29112 - #29113 PCR, pCW3) / This study
pJIR3170 / pKT25  (Asp718/BamHI: 0.4 kb TcpE product #29125 - #29126 PCR, pCW3) / This study
pJIR3171 / pKT25  (Asp718/BamHI: 0.5 kb TcpI #29120 - #29123 PCR, pCW3) / This study
pJIR3176 / pKT25  (Asp718/BamHI: 2.1 kb TcpH311-450 #28691 - #28692 PCR, pJIR3115) / This study
pJIR3175 / pKT25  (Asp718/BamHI: 1.0 kb TcpC #29118 - #29127 PCR, pCW3) / This study
pJIR3177 / pUT18C  (Asp718/BamHI: 1.0 kb TcpC #29118 - #29127 PCR, pCW3) / This study
pJIR3178 / pKT25  (Asp718/BamHI: 0.6 kb TcpJ #29122 - #29206 PCR, extra base T at 37547 bp in pCW3, pCW3) / This study
pJIR3180 / pKT25  (Asp718/BamHI: 1.9 kb TcpH202-832 #29228 - #28692 PCR, pCW3) / This study
pJIR3190 / pET22b+  (NdeI/XhoI: 2.5 kb TcpH1-832 #29343 - #26521 PCR, pCW3) / This study
pJIR3191 / pGEX-KG  (BamHI/XhoI: 2.5 kb TcpH1-832 #29342 - #26508 PCR, pCW3) / This study

* ApR, CmR and EmR represent resistance to ampicillin, chloramphenicol and erythromycin antibiotics respectively. Sequences of oligonucleotide primers are available upon request.