Supplemental Table I: a List of the Drug Transporters Evaluated and Their Gene Expression

Supplemental Table I: A list of the drug transporters evaluated and their gene expression in the RPMI 2650 cell line.

Symbol / Description / A-L1 / L-L1 / A-L3 / L-L3
ABC transporters / ABCA1 / ATP-binding cassette, sub-family A (ABC1), member 1 / 0.0022533 / 0.0011397 / 0.0060869 / 0.0052324
ABCA2 / ATP-binding cassette, sub-family A (ABC1), member 2 / 0.0012588 / 0.0015786 / 0.0008680 / 0.0017261
ABCA3 / ATP-binding cassette, sub-family A (ABC1), member 3 / 0.0001323 / 0.0001603 / 0.0006716 / 0.0005203
ABCA4 / ATP-binding cassette, sub-family A (ABC1), member 4 / 0.1163262 / 0.1128817 / 0.1182509 / 0.0494355
ABCA9 / ATP-binding cassette, sub-family A (ABC1), member 9 / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
ABCA12 / ATP-binding cassette, sub-family A (ABC1), member 12 / 0.0031866 / 0.0016686 / 0.0004310 / 0.0005167
ABCA13 / ATP-binding cassette, sub-family A (ABC1), member 13 / 0.0000284 / 0.0000250 / 0.0000231 / 0.0000250
ABCB1 / ATP-binding cassette, sub-family B (MDR/TAP), member 1 (MDR1) / 0.0000556 / 0.0000250 / 0.0007099 / 0.0000578
ABCB2 / ATP-binding cassette, sub-family B (MDR/TAP), Transporter 1 (TAP1) / 0.0002702 / 0.0001320 / 0.0006398 / 0.0001356
ABCB3 / ATP-binding cassette, sub-family B (MDR/TAP), Transporter 2 (TAP2) / 0.0037634 / 0.0019435 / 0.0067538 / 0.0021250
ABCB4 / ATP-binding cassette, sub-family B (MDR/TAP), member 4 (MDR3) / 0.0009808 / 0.0003892 / 0.0007877 / 0.0005424
ABCB5 / ATP-binding cassette, sub-family B (MDR/TAP), member 5 / 0.0000592 / 0.0000250 / 0.0000318 / 0.0000250
ABCB6 / ATP-binding cassette, sub-family B (MDR/TAP), member 6 / 0.0193201 / 0.0215358 / 0.0257359 / 0.0354442
ABCB11 / ATP-binding cassette, sub-family B (MDR/TAP), member 11 / 0.0009540 / 0.0007016 / 0.0004985 / 0.0002566
ABCC1 / ATP-binding cassette, sub-family C (CFTR/MRP), member 1 (MRP1) / 0.1272950 / 0.0855484 / 0.0738081 / 0.0872738
ABCC2 / ATP-binding cassette, sub-family C (CFTR/MRP), member 2 (MRP2) / 0.0001136 / 0.0000633 / 0.0003993 / 0.0002656
ABCC3 / ATP-binding cassette, sub-family C (CFTR/MRP), member 3 (MRP3) / 0.0005633 / 0.0007016 / 0.0074421 / 0.0005854
ABCC4 / ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (MRP4) / 0.0123980 / 0.0075092 / 0.0084310 / 0.0055308
ABCC5 / ATP-binding cassette, sub-family C (CFTR/MRP), member 5 (MRP5) / 0.0290815 / 0.0150185 / 0.0241794 / 0.0252371
ABCC6 / ATP-binding cassette, sub-family C (CFTR/MRP), member 6 (MRP6) / 0.0003082 / 0.0001614 / 0.0002510 / 0.0001028
ABCC10 / ATP-binding cassette, sub-family C (CFTR/MRP), member 10 (MRP7) / 0.0009408 / 0.0007016 / 0.0015430 / 0.0009314
ABCC11 / ATP-binding cassette, sub-family C (CFTR/MRP), member 11 (MRP8) / 0.0001820 / 0.0001118 / 0.0001812 / 0.0000620
ABCC12 / ATP-binding cassette, sub-family C (CFTR/MRP), member 12 (MRP9) / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
ABCD1 / ATP-binding cassette, sub-family D (ALD), member 1 / 0.0006426 / 0.0006279 / 0.0004134 / 0.0009314
ABCD3 / ATP-binding cassette, sub-family D (ALD), member 3 / 0.0581631 / 0.0885652 / 0.0669823 / 0.0587891
ABCD4 / ATP-binding cassette, sub-family D (ALD), member 4 / 0.0058646 / 0.0070064 / 0.0052260 / 0.0052688
ABCF1 / ATP-binding cassette, sub-family F (GCN20), member 1 / 0.0569661 / 0.0335599 / 0.0665196 / 0.0537233
ABCG2 / ATP-binding cassette, sub-family G (WHITE), member 2 (BCRP) / 0.0004067 / 0.0007467 / 0.0002843 / 0.0003225
ABCG8 / ATP-binding cassette, sub-family G (WHITE), member 8 / 0.0003213 / 0.0001946 / 0.0003244 / 0.0003529
SLC transporters / SLC2A1 / Solute carrier family 2 (facilitated glucose transporter), member 1 (GLUT1) / 0.0138521 / 0.0085071 / 0.0115171 / 0.0278089
SLC2A2 / Solute carrier family 2 (facilitated glucose transporter), member 2 (GLUT2) / 0.0001783 / 0.0002001 / 0.0002262 / 0.0003604
SLC2A3 / Solute carrier family 2 (facilitated glucose transporter), member 3 (GLUT3) / 0.0040616 / 0.0034550 / 0.0037730 / 0.0026344
SLC3A1 / Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 / 0.0978183 / 0.0962469 / 0.0927778 / 0.0884920
SLC3A2 / Solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 / 0.0610548 / 0.0364708 / 0.1258628 / 0.0749302
SLC5A1 / Solute carrier family 5 (sodium/glucose cotransporter), member 1 / 0.0001235 / 0.0000555 / 0.0000291 / 0.0000920
SLC5A4 / Solute carrier family 5 (low affinity glucose cotransporter), member 4 / 0.0012329 / 0.0010634 / 0.0013526 / 0.0008751
SLC7A5 / Solute carrier family 7 (amino acid transporter light chain, L system), member 5 / 0.0180263 / 0.0085071 / 0.0299754 / 0.0173574
SLC7A6 / Solute carrier family 7 (amino acid transporter light chain, y+L system), member 6 / 0.0991838 / 0.0832090 / 0.0647006 / 0.0533522
SLC7A7 / Solute carrier family 7 (amino acid transporter light chain, y+L system), member 7 / 0.0145408 / 0.0146078 / 0.0096178 / 0.0122735
SLC7A8 / Solute carrier family 7 (amino acid transporter light chain, L system), member 8 / 0.0402811 / 0.0380195 / 0.0669823 / 0.0652306
SLC7A9 / Solute carrier family 7 (glycoprotein-associated amino acid transporter light chain, bo,+ system), member 9 / 0.0008778 / 0.0005859 / 0.0021372 / 0.0018119
SLC7A11 / Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 / 0.0772805 / 0.0955821 / 0.0423916 / 0.0560047
SLC10A1 / Solute carrier family 10 (sodium/bile acid cotransporter family), member 1 / 0.0010883 / 0.0011718 / 0.0011374 / 0.0020385
SLC10A2 / Solute carrier family 10 (sodium/bile acid cotransporter family), member 2 / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLC15A1 / Solute carrier family 15 (oligopeptide transporter), member 1 (PEPT1) / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLC15A2 / Solute carrier family 15 (H+/peptide transporter), member 2 (PEPT2) / 0.0029323 / 0.0020686 / 0.0017847 / 0.0022307
SLC16A1 / Solute carrier family 16, member 1 (monocarboxylic acid transporter 1) / 0.0200014 / 0.0101167 / 0.0116779 / 0.0167661
SLC16A2 / Solute carrier family 16, member 2 (monocarboxylic acid transporter 8) / 0.0009088 / 0.0007467 / 0.0006905 / 0.0016905
SLC16A3 / Solute carrier family 16, member 3 (monocarboxylic acid transporter 4) / 0.0000284 / 0.0000342 / 0.0000246 / 0.0000841
SLC19A1 / Solute carrier family 19 (folate transporter), member 1 / 0.0003742 / 0.0002929 / 0.0002527 / 0.0002907
SLC19A2 / Solute carrier family 19 (thiamine transporter), member 2 / 0.0640902 / 0.0545183 / 0.0748384 / 0.0600243
SLC19A3 / Solute carrier family 19, member 3 / 0.0004095 / 0.0003557 / 0.0012620 / 0.0010052
SLC22A1 / Solute carrier family 22 (organic cation transporter), member 1 / 0.0000982 / 0.0000332 / 0.0001577 / 0.0001681
SLC22A2 / Solute carrier family 22 (organic cation transporter), member 2 / 0.0002368 / 0.0008578 / 0.0012446 / 0.0044613
SLC22A3 / Solute carrier family 22 (extraneuronal monoamine transporter), member 3 / 0.0000771 / 0.0000250 / 0.0004370 / 0.0002548
SLC22A6 / Solute carrier family 22 (organic anion transporter), member 6 / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLC22A7 / Solute carrier family 22 (organic anion transporter), member 7 / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLC22A8 / Solute carrier family 22 (organic anion transporter), member 8 / 0.0002076 / 0.0000250 / 0.0000227 / 0.0010848
SLC22A9 / Solute carrier family 22 (organic anion transporter), member 9 / 0.0000284 / 0.0000250 / 0.0000438 / 0.0000295
SLC25A13 / Solute carrier family 25, member 13 (citrin)+ / 0.1077865 / 0.0694869 / 0.0480248 / 0.0522542
SLC28A1 / Solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLC28A2 / Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 / 0.0017926 / 0.0014426 / 0.0009698 / 0.0009510
SLC28A3 / Solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 / 0.0001243 / 0.0001275 / 0.0001472 / 0.0001891
SLC29A1 / Solute carrier family 29 (nucleoside transporters), member 1 / 0.0015390 / 0.0020686 / 0.0004917 / 0.0011708
SLC29A2 / Solute carrier family 29 (nucleoside transporters), member 2 / 0.0025883 / 0.0026184 / 0.0009367 / 0.0020669
SLC31A1 / Solute carrier family 31 (copper transporters), member 1 / 0.0246247 / 0.0208023 / 0.0196398 / 0.0159720
SLC38A2 / Solute carrier family 38, member 2 / 0.2545900 / 0.1305690 / 0.2161228 / 0.2351563
SLC38A5 / Solute carrier family 38, member 5 / 0.0001192 / 0.0000514 / 0.0000778 / 0.0002041
SLCO1A2 / Solute carrier organic anion transporter family, member 1A2 (SLC21A3, OATP1A2) / 0.0561818 / 0.0560510 / 0.0111248 / 0.0369493
SLCO1B1 / Solute carrier organic anion transporter family, member 1B1 (SLC21A6, OATP1B1) / 0.0020167 / 0.0013554 / 0.0006443 / 0.0015449
SLCO1B3 / Solute carrier organic anion transporter family, member 1B3 (SLC21A8, OATP1B3) / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLCO2A1 / Solute carrier organic anion transporter family, member 2A1 (SLC21A2, OATP2A1) / 0.0040898 / 0.0010857 / 0.0009238 / 0.0008630
SLCO2B1 / Solute carrier organic anion transporter family, member 2B1 (SLC21A9, OATP2B1) / 0.0000284 / 0.0000250 / 0.0000227 / 0.0000250
SLCO3A1 / Solute carrier organic anion transporter family, member 3A1 (SLC21A11, OATP3A1) / 0.0133802 / 0.0081606 / 0.0129574 / 0.0093016
SLCO4A1 / Solute carrier organic anion transporter family, member 4A1 (SLC21A12, OATP4A1) / 0.0032762 / 0.0029458 / 0.0245170 / 0.0120209
Other transporters / AQP1 / Aquaporin 1 (Colton blood group) / 0.0278969 / 0.0183622 / 0.0255581 / 0.0125314
AQP7 / Aquaporin 7 / 0.0002977 / 0.0003319 / 0.0002458 / 0.0003270
AQP9 / Aquaporin 9 / 0.0001640 / 0.0000250 / 0.0000389 / 0.0000250
ATP6V0C / ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c / 0.1373804 / 0.0867426 / 0.1080614 / 0.1369472
ATP7A / ATPase, Cu++ transporting, alpha polypeptide / 0.0074748 / 0.0085071 / 0.0080876 / 0.0091735
ATP7B / ATPase, Cu++ transporting, beta polypeptide / 0.0002225 / 0.0001072 / 0.0001863 / 0.0002345
MVP / Major vault protein / 0.0005594 / 0.0005317 / 0.0010612 / 0.0013081
VDAC1 / Voltage-dependent anion channel 1 / 0.6225440 / 0.6429970 / 0.4322457 / 0.5040685
VDAC2 / Voltage-dependent anion channel 2 / 0.5971842 / 0.3958111 / 0.6506364 / 0.4238694

Data analysis was performed using the ΔCq method, where the ΔCq value is normalized to the geometric mean of all five reference genes (B2B, HPRT1, RPL13A, GAPDH, and ACTB). A-L, air-liquid interface; L-L, liquid-liquid interface; 1, one wk in culture; 3, three wks in culture.