Supplemental Table 3. Protein Identified Using Mass Spectrometry

Supplemental table 3. Protein identified using Mass Spectrometry

spot ID / 1856
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / gi|1673456 / 19363.6 / 8.98 / 7 / 271 / 100 / 220 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
906.5043 / 906.5126 / .0083 / 9 / 76 / 82 / QVDYLLR
919.4996 / 919.5052 / .0056 / 6 / 147 / 154 / VIGFDNVR
919.4996 / 919.5052 / .0056 / 6 / 147 / 154 / VIGFDNVR / 34 / 0
930.4178 / 930.4383 / .0205 / 22 / 112 / 117 / YWTMWK
1350.6511 / 1350.6667 / .0156 / 12 / 83 / 93 / NGWIPCLEFSK
1448.5747 / 1448.5824 / .0077 / 5 / 100 / 111 / ENSTSPCYYDGR
1448.5747 / 1448.5824 / .0077 / 5 / 100 / 111 / ENSTSPCYYDGR / 73 / 99.984
1730.8496 / 1730.8541 / .0045 / 3 / 132 / 146 / ELQEAIAAYPDAFHR
1730.8496 / 1730.8541 / .0045 / 3 / 132 / 146 / ELQEAIAAYPDAFHR / 89 / 100
1811.8633 / 1811.8768 / .0135 / 7 / 155 / 170 / QTQCVSFIAYKPPGSE
1811.8633 / 1811.8768 / .014 / 7 / 155 / 170 / QTQCVSFIAYKPPGSE / 23 / 0
spot ID / 1313
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
proteasome subunit alpha type 1 / gi|226531007 / 30282.0 / 5.19 / 12 / 403 / 100 / 294 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
821.4628 / 821.4457 / -.0171 / -21 / 170 / 175 / TFLERR
821.4628 / 821.4457 / -.0171 / -21 / 170 / 175 / TFLERR / 8 / 0
947.4727 / 947.4926 / .0199 / 21 / 31 / 39 / QGSACVGLR / Carbamidomethyl (C)[5]
1150.6216 / 1150.6259 / .0043 / 4 / 165 / 174 / SQAAKTFLER
1289.6631 / 1289.691 / .0279 / 22 / 112 / 122 / LADKAQVCTQR / Carbamidomethyl (C)[8]
1289.6631 / 1289.691 / .0279 / 22 / 112 / 122 / LADKAQVCTQR / 53 / 98.393 / Carbamidomethyl (C)[8]
1363.6998 / 1363.7334 / .0336 / 25 / 204 / 216 / LSSSNCTVAIVGR / Carbamidomethyl (C)[6]
1443.7189 / 1443.7826 / .0637 / 44 / 19 / 30 / LFQVEYAMEAVK / Oxidation (M)[8]
1593.8344 / 1593.8939 / .0595 / 37 / 66 / 82 / VADHAGVALAGLTADGR
1593.8344 / 1593.8939 / .0595 / 37 / 66 / 82 / VADHAGVALAGLTADGR / 100 / 100
1635.7683 / 1635.8374 / .0691 / 42 / 218 / 232 / EDGTIEPFEMIGAAR
1651.7632 / 1651.8276 / .0644 / 39 / 218 / 232 / EDGTIEPFEMIGAAR / Oxidation (M)[10]
1651.7632 / 1651.8276 / .0644 / 39 / 218 / 232 / EDGTIEPFEMIGAAR / 47 / 94.504 / Oxidation (M)[10]
1715.8275 / 1715.9191 / .0916 / 53 / 176 / 189 / FQGYNDYTPEQLIK
1763.8633 / 1763.9391 / .0758 / 43 / 217 / 232 / KEDGTIEPFEMIGAAR
1767.7933 / 1767.8767 / .0834 / 47 / 4 / 18 / NQYDTDVTTWSPQGR
1767.7933 / 1767.8767 / .0834 / 47 / 4 / 18 / NQYDTDVTTWSPQGR / 69 / 99.964
1779.8582 / 1779.9387 / .0805 / 45 / 217 / 232 / KEDGTIEPFEMIGAAR / Oxidation (M)[11]
1779.8582 / 1779.9387 / .0805 / 45 / 217 / 232 / KEDGTIEPFEMIGAAR / 25 / 0 / Oxidation (M)[11]
2132.0229 / 2132.1455 / .1226 / 58 / 90 / 107 / NECINHSFVYEAPLPVSR / Carbamidomethyl (C)[3]
spot ID / 2209
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
RING-box protein / gi|83701143 / 130821 / 6.64 / 2 / 152 / 100 / 140 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
852.4462 / 852.4387 / -.0075 / -9 / 81 / 88 / QVCPLDNSEWEFQK
852.4462 / 852.4387 / -.0075 / -9 / 81 / 88 / QVCPLDNSEWEFQK / 39 / 72.847
2558.1794 / 2558.2104 / .031 / 12 / 89 / 112 / KWNAVALWAWDIVVDNCAICR
2558.1794 / 2558.2104 / .031 / 12 / 89 / 112 / KWNAVALWAWDIVVDNCAICR / 101 / 100
spot ID / 2080
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
putative growth-regulating factor 1 / gi|310696360 / 23725.5 / 5.05 / 7 / 118 / 99.973 / 70 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
805.3733 / 805.3849 / .0116 / 14 / 214 / 219 / RCQEGR / Carbamidomethyl (C)[2]
806.3792 / 806.3889 / .0097 / 12 / 188 / 195 / TDGFPGGR
836.3818 / 836.4128 / .031 / 37 / 1 / 8 / MTAEGEAK
852.3767 / 852.4277 / .051 / 60 / 1 / 8 / MTAEGEAK / 43 / 99.991 / Oxidation (M)[1]
856.4498 / 856.4838 / .034 / 40 / 199 / 204 / FCFRVK / Carbamidomethyl (C)[2]
895.4268 / 895.4277 / .0009 / 1 / 177 / 184 / NPEPEPGR
952.4747 / 952.4587 / -.016 / -17 / 208 / 214 / SWQGYRR
952.4747 / 952.4587 / -.016 / -17 / 207 / 213 / SWQGYRR / 27 / 0
spot ID / 2048
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit / gi|11467200 / 53294.6 / 6.33 / 14 / 597 / 100 / 415 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
802.4305 / 802.4281 / -.0024 / -3 / 440 / 446 / EGNEIIK
930.4752 / 930.4774 / .0022 / 2 / 432 / 439 / NEGRDLAR
930.4752 / 930.4774 / .0022 / 2 / 432 / 439 / NEGRDLAR / 30 / 0
983.5197 / 983.5307 / .011 / 11 / 464 / 471 / EIKFDGFK
983.5197 / 983.5307 / .011 / 11 / 464 / 471 / EIKFDGFK / 74 / 99.983
1021.5312 / 1021.4886 / -.0426 / -42 / 33 / 41 / DTDILAAFR
1037.4899 / 1037.4928 / .0029 / 3 / 351 / 358 / DDFIEKDR
1037.4899 / 1037.4928 / .0029 / 3 / 351 / 358 / DDFIEKDR / 58 / 99.366
1116.583 / 1116.6075 / .0245 / 22 / 422 / 431 / VALEACVQAR / Carbamidomethyl (C)[6]
1116.583 / 1116.6075 / .0245 / 22 / 422 / 431 / VALEACVQAR / 87 / 100 / Carbamidomethyl (C)[6]
1257.6797 / 1257.697 / .0173 / 14 / 436 / 446 / DLAREGNEIIK
1275.7307 / 1275.7511 / .0204 / 16 / 340 / 350 / EITLGFVDLLR
1275.7307 / 1275.7511 / .0204 / 16 / 340 / 350 / EITLGFVDLLR / 108 / 100
1481.7167 / 1481.7158 / -.0009 / -1 / 320 / 334 / MSGGDHIHSGTVVGK
1481.7167 / 1481.7158 / -.0009 / -1 / 320 / 334 / MSGGDHIHSGTVVGK / 78 / 99.993
1497.7115 / 1497.7169 / .0054 / 4 / 320 / 334 / MSGGDHIHSGTVVGK / Oxidation (M)[1]
1534.7358 / 1534.7324 / -.0034 / -2 / 451 / 463 / WSAELAAACEIWK / Carbamidomethyl (C)[9]
1809.7974 / 1809.8157 / .0183 / 10 / 202 / 215 / DDENVNSQPFMRWR / Oxidation (M)[11]
1860.0226 / 1860.01 / -.0126 / -7 / 335 / 350 / LEGEREITLGFVDLLR
1860.0226 / 1860.01 / -.0126 / -7 / 335 / 350 / LEGEREITLGFVDLLR / 67 / 99.91
2023.0747 / 2023.0599 / -.0148 / -7 / 340 / 356 / EITLGFVDLLRDDFIEK
2066.0085 / 2065.9863 / -.0222 / -11 / 320 / 339 / MSGGDHIHSGTVVGKLEGER
2082.0034 / 2082.011 / .0076 / 4 / 320 / 339 / MSGGDHIHSGTVVGKLEGER / Oxidation (M)[1]
spot ID / 1947
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
eukaryotic translation initiation factor 5A / gi|162458009 / 17713.8 / 5.61 / 9 / 361 / 100 / 274 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
1120.5746 / 1120.5852 / .0106 / 9 / 20 / 29 / TYPQQAGTVR
1120.5746 / 1120.5852 / .0106 / 9 / 20 / 29 / TYPQQAGTVR / 62 / 99.779
1248.6696 / 1248.6696 / 0 / 0 / 20 / 30 / TYPQQAGTVRK
1248.6696 / 1248.6696 / 0 / 0 / 20 / 30 / TYPQQAGTVRK / 56 / 99.211
1420.7042 / 1420.698 / -.0062 / -4 / 59 / 70 / CHFVAIDIFNGK
1503.7948 / 1503.8026 / .0078 / 5 / 38 / 50 / NRPCKVVEVSTSK
1720.8613 / 1720.8656 / .0043 / 2 / 13 / 29 / ADAGASKTYPQQAGTVR
1720.8613 / 1720.8656 / .0043 / 2 / 13 / 29 / ADAGASKTYPQQAGTVR / 66 / 99.923
1826.9858 / 1826.9922 / .0064 / 4 / 114 / 129 / DDLRLPTDETLVAQIK
1826.9858 / 1826.9922 / .0064 / 4 / 114 / 129 / DDLRLPTDETLVAQIK / 29 / 0
2102.0083 / 2102.0225 / .0142 / 7 / 72 / 89 / LEDIVPSSHNCDIPHVNR
2102.0083 / 2102.0225 / .0142 / 7 / 72 / 89 / LEDIVPSSHNCDIPHVNR / 34 / 0
2230.1033 / 2230.1025 / -.0008 / 0 / 71 / 89 / KLEDIVPSSHNCDIPHVNR
2230.1033 / 2230.1025 / -.0008 / 0 / 71 / 89 / KLEDIVPSSHNCDIPHVNR / 26 / 0
2603.3057 / 2603.3032 / -.003 / - 1 / 137 / 160 / DLVVTVQSAMGEEQICALKDVGPK
spot ID / 1955
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
superoxide dismutase [Cu-Zn] 4AP / gi|162463249 / 15219.5 / 5.65 / 7 / 255 / 100 / 190 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
987.5292 / 987.5104 / -.0188 / -19 / 143 / 152 / VACGIIGLQG
987.5292 / 987.5104 / -.0188 / -19 / 143 / 152 / VACGIIGLQG / 39 / 64.665
1116.626 / 1116.5929 / -.0331 / -30 / 4 / 15 / AVAVLGSSEGVK
1153.4868 / 1153.4907 / .0039 / 3 / 69 / 78 / EHGAPEDENR
1335.6903 / 1335.7133 / .023 / 17 / 115 / 127 / AVVVHADPDDLGK
1687.8545 / 1687.8744 / .0199 / 12 / 135 / 152 / STGNAGGRVACGIIGLQG
1687.8545 / 1687.8744 / .0199 / 12 / 135 / 152 / STGNAGGRVACGIIGLQG
2044.0459 / 2044.0656 / .0197 / 10 / 115 / 134 / AVVVHADPDDLGKGGHELSK
2044.0459 / 2044.0656 / .0197 / 10 / 115 / 134 / AVVVHADPDDLGKGGHELSK / 108 / 100
3514.7886 / 3514.8032 / .0146 / 4 / 79 / 114 / HAGDLGNVTAGADGVAN
INVTDSQIPLTGPNSIIGR
3514.7886 / 3514.8032 / .015 / 4 / 79 / 114 / HAGDLGNVTAGADGVAN / 43 / 85
spot ID / 2067
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
oxygen-evolving enhancer protein 3-1 / gi|195611174 / 22669.3 / 9.80 / 8 / 270 / 100 / 210 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
1128.6047 / 1128.6049 / .0002 / 0 / 145 / 153 / ASYLRYDLK
1132.5997 / 1132.5946 / -.0051 / -5 / 92 / 100 / DFDLPLKER
1132.5997 / 1132.5946 / -.0051 / -5 / 92 / 100 / DFDLPLKER / 40 / 71.015
1261.6324 / 1261.6335 / .0011 / 1 / 133 / 142 / AWPYVQNDLR
1261.6324 / 1261.6335 / .0011 / 1 / 133 / 142 / AWPYVQNDLR / 16 / 0
1442.7162 / 1442.7117 / -.0045 / -3 / 189 / 201 / STPEAEKYFAATK
1445.7272 / 1445.726 / -.0012 / -1 / 174 / 186 / LFSTIDDLDHAAK
1445.7272 / 1445.726 / -.0012 / -1 / 174 / 186 / LFSTIDDLDHAAK / 38 / 48.929
1571.858 / 1571.8593 / .0013 / 1 / 101 / 114 / FYLQPLPPAEAVAR
1571.858 / 1571.8593 / .0013 / 1 / 101 / 114 / FYLQPLPPAEAVAR / 59 / 99.58
1582.8475 / 1582.7803 / -.0672 / -42 / 196 / 210 / YFAATKDALGDVLAK
2002.0353 / 2002.0341 / -.0012 / -1 / 71 / 91 / IGAPPPPSGGLPGTLNSDQAR
2002.0353 / 2002.0341 / -.001 / - 1 / 71 / 91 / IGAPPPPSGGLPGTLNSDQAR / 56 / 99
spot ID / 2059
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
oxygen-evolving enhancer protein 3-1 / gi|195611174 / 22669.3 / 9.80 / 12 / 317 / 100 / 215 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
847.456 / 847.3815 / -.0745 / -88 / 92 / 98 / DFDLPLK
878.5206 / 878.4855 / -.0351 / -40 / 143 / 149 / LRASYLR
1003.5782 / 1003.5105 / -.0677 / -67 / 165 / 173 / QSLKELTGK
1128.6047 / 1128.6021 / -.0026 / -2 / 145 / 153 / ASYLRYDLK
1132.5997 / 1132.5958 / -.0039 / -3 / 92 / 100 / DFDLPLKER
1132.5997 / 1132.5958 / -.0039 / -3 / 92 / 100 / DFDLPLKER / 44 / 85.793
1261.6324 / 1261.6367 / .0043 / 3 / 133 / 142 / AWPYVQNDLR
1261.6324 / 1261.6367 / .0043 / 3 / 133 / 142 / AWPYVQNDLR / 21 / 0
1389.7274 / 1389.7255 / -.0019 / -1 / 132 / 142 / KAWPYVQNDLR
1442.7162 / 1442.7157 / -.0005 / 0 / 189 / 201 / STPEAEKYFAATK
1445.7272 / 1445.7302 / .003 / 2 / 174 / 186 / LFSTIDDLDHAAK
1571.858 / 1571.8638 / .0058 / 4 / 101 / 114 / FYLQPLPPAEAVAR
1571.858 / 1571.8638 / .0058 / 4 / 101 / 114 / FYLQPLPPAEAVAR / 68 / 99.943
1582.8475 / 1582.7794 / -.0681 / -43 / 196 / 210 / YFAATKDALGDVLAK
2002.0353 / 2002.0364 / .0011 / 1 / 71 / 91 / IGAPPPPSGGLPGTLNSDQAR
2002.0353 / 2002.0364 / .001 / 1 / 71 / 91 / IGAPPPPSGGLPGTLNSDQAR / 83 / 100
spot ID / 1895
Protein Name / Accession No. / Protein MW / Protein PI / Pep.Count / Protein Score / Protein Score C. I. % / Total Ion Score / Total Ion C. I. %
peptidyl-prolyl cis-trans isomerase / gi|226491656 / 26372.5 / 9.28 / 10 / 203 / 100 / 124 / 100
Peptide Information
Calc. Mass / Obsrv. Mass / ± da / ± ppm / Start Seq. / End Seq. / Sequence / Ion Score / C. I. % / Modification
804.4097 / 804.4573 / .0476 / 59 / 72 / 78 / EAELQSK
844.4312 / 844.4229 / -.0083 / -10 / 199 / 205 / TPWLDGR
844.4312 / 844.4229 / -.0083 / -10 / 199 / 205 / TPWLDGR / 38 / 47.987
1028.5048 / 1028.4744 / -.0304 / -30 / 162 / 169 / TFKDENFK
1028.5048 / 1028.4744 / -.0304 / -30 / 162 / 169 / TFKDENFK / 32 / 0
1177.6688 / 1177.6031 / -.0657 / -56 / 16 / 26 / SAGQRFLLASK
1242.6014 / 1242.6202 / .0188 / 15 / 125 / 135 / GFGYKGSSFHR
1330.646 / 1330.6455 / -.0005 / 0 / 118 / 129 / ALCTGEKGFGYK / Carbamidomethyl (C)[3]
1408.7472 / 1408.7301 / -.0171 / -12 / 83 / 95 / VYFDISIGNPVGK
1587.736 / 1587.7379 / .0019 / 1 / 136 / 149 / VISDFMIQGGDFDK / Oxidation (M)[6]
2037.0288 / 2037.0322 / .0034 / 2 / 100 / 117 / IVIGLYGDDVPQTTENFR
2143.0125 / 2143.0107 / -.0018 / -1 / 136 / 156 / VISDFMIQGGDFDKGNGTGGK
2159.0073 / 2159.0132 / .0059 / 3 / 136 / 156 / VISDFMIQGGDFDKGNGTGGK / Oxidation (M)[6]
2159.0073 / 2159.0132 / .0059 / 3 / 136 / 156 / VISDFMIQGGDFDKGNGTGGK / 54 / 98.729 / Oxidation (M)[6]