Supplemental Table 2: Analytical Conditions for Fluvastatin and Rifampicin

Supplemental Tables

Supplemental Table 1: Oligonucleotides used for the real-time PCR, 5’-RACE PCR,bisulfite PCR, and construction of plasmid vector

Oligonucleotide / Orientation / Sequence (5 to 3)
Primers used for the real-time PCR
OATP1B3
(primer set A) / forward / AATGTATGGCCACGTTACTG
reverse / TCCATCAATTAAACCAGCAAGA
OATP1B3
(primer set B) / forward / TCAAACCAAGCATCAGCAAC
reverse / AATGACAGGGCTGCCAAG
MeCP2 / forward / CCCCACCCTGCCTGAA
reverse / GATGTGTCGCCTACCTTTTCG
MBD1 / forward / CACCCTCTTCGACTTCAAACAAG
reverse / CAACCTGACGTTTCCGAGTCTT
MBD2 / forward / AACCCTGCTGTTTGGCTTAAC
reverse / TATCAGCAGCTCGCGACAAGATG
MBD4 / forward / ATCAGCCTTGCTAAAGCTAGGG
reverse / TGAGACAGGGTCTCTCTCTGTCAT
GAPDH / forward / AATGACCCCTTCATTGAC
reverse / TCCACGACGTACTCAGCGC
Primer used for the 5’-RACE PCR
OATP1B3 / reverse / GCAATATAGCTGAATGACAGGGCTGC
Primers used for the bisulfite PCR
OATP1B3 / forward / TATGTGTGTTTATGTGAAGAGATT
reverse / ACTAAACACAAAACAAATAAATAACTA
Primers used for theconstruction of plasmid vector
forward / ATAAGAATGCGGCCGCCCACCATGTTCTTGGCAGCCCTGTC
reverse / CGGGATCCGTTGGCAGCAGCATTGT

Supplemental Table 2: Analytical conditions for fluvastatin and rifampicin

Fluvastatin
Analytical column / Inertsil ODS-3 (5 µm, 2.1 mm × 50 mm; GL sciences, Tokyo, Japan)
Mobile Phase A / 0.1% foric acid
Mobile Phase B / Acetonitrile
Gradient condition
(B concentration %) / 0 min, 20%; 1 min, 98%; 1.5 min, 98%; 1.51 min, 20%
Flow rate(mL/min) / 0.4
Retention time(min) / 1.7
Ion mode / Negative
Mass-to-charge / 410.07→347.90
Rifampicin
Analytical column / Inertsil ODS-3 (5 µm, 2.1 mm × 50 mm; GL sciences, Tokyo, Japan)
Mobile Phase A / 0.1% foric acid
Mobile Phase B / Acetonitrile
Gradient condition
(B concentration %) / 0 min, 5%; 0.5 min, 5%; 1.5 min, 90%; 2 min, 90%; 2.01 min, 5%
Flow rate(mL/min) / 0.5
Retention time(min) / 1.8
Ion mode / Positive
Mass-to-charge / 823.46→791.30

Supplemental Table 3: Oligonucleotides used for the siRNA knockdown of MBDs

Oligonucleotide / Strand / Sequence (5 to 3)
MeCP2-siRNA-1 / sense / CCACGUCAGAGGGUGUGCAGGUGAA
antisense / UUCACCUGCACACCCUCUGACGUGG
MeCP2-siRNA-2 / sense / AAAGUCCUGGGAAGCUCCUUGUCAA
antisense / UUGACAAGGAGCUUCCCAGGACUUU
MeCP2-siRNA-3 / sense / GACACAUCCCUGGACCCUAAUGAUU
antisense / AAUCAUUAGGGUCCAGGGAUGUGUC
MBD1-siRNA-1 / sense / GGAAGCGCCGCGAAGUCUUUCGCAA
antisense / UUGCGAAAGACUUCGCGGCGCUUCC
MBD1-siRNA-2 / sense / GAAGCGAAAGAAGCCUUCAAGGCCA
antisense / UGGCCUUGAAGGCUUCUUUCGCUUC
MBD1-siRNA-3 / sense / CGAGAUACAGCAGUCUGGUUGCCAA
antisense / UUGGCAACCAGACUGCUGUAUCUCG
MBD2-siRNA-1 / sense / GGAAGAAGGAGGAAGUGAUCCGAAA
antisense / UUUCGGAUCACUUCCUCCUUCUUCC
MBD2-siRNA-2 / sense / UCUACUACUUCAGUCCAAGUGGUAA
antisense / UUACCACUUGGACUGAAGUAGUAGA
MBD2-siRNA-3 / sense / GAACUGGAAAGAUGAUGCCUAGUAA
antisense / UUACUAGGCAUCAUCUUUCCAGUUC
MBD4-siRNA-1 / sense / CCCUUGCUACAAGAACCCAUCGCUU
antisense / AAGCGAUGGGUUCUUGUAGCAAGGG
MBD4-siRNA-2 / sense / GAAGAUGAGGGUGUUGAUGAUGUUA
antisense / UAACAUCAUCAACACCCUCAUCUUC
MBD4-siRNA-3 / sense / CAACGAGAAGUAUGAGGAUACCUUU
antisense / AAAGGUAUCCUCAUACUUCUCGUUG