Supplemental Materials : Presence and Distribution of Macrolides-Lincosamide-Streptogramin

Supplemental Materials: Presence and distribution of Macrolides-Lincosamide-Streptogramin resistance genes and potential indicator ARGs in the university ponds in Guangzhou, China

Mianzhi Wang, Jing Sun, WeiXin Zhong, Wenguang Xiong, Zhenling Zeng, Yongxue Sun *

Corresponding

Yongxue Sun, National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China

E-mail: .

Pages: 11

Tables: 9

Table S1

Primers for the detection and quantification of antimicrobial resistance genes and mobile elements.

Genes / Primer sequence(5’-3’) / ASa / MTb / Ref
Forward primer / Reverse primer
ereB / TCGAGTAAAAGTTCGCCTTGA / TAAAGCCCGACATAGCTTGAA / 137 / 58.2/55 / [1]
ermA / GGTTTGCTATTGATGGTGGAA / GAACGCGATATTCACGGTTTA / 190 / 55/55 / [1]
ermB / CCGTGCGTCTGACATCTATCT / GTGGTATGGCGGGTAAGTTTT / 189 / 56.8/55 / [1]
ermF / TAGATATTGGGGCAGGCAAG / GGAAATTGCGGAACTGCAAA / 200 / 58/56 / [2]
mefA / ATACCCCAGCACTCAATTCG / CAATCACAGCACCCAATACG / 186 / 59/58 / [2]
mphA / AGTTCGTGGTGAACGACAAG / AGTCGATCATCCCGCTGAC / 153 / 58.2/58 / [3]
vatB / ATTATGAATGGAGCAAACCATAGAATG / ACCAATCCACACATCATTTCC / 147 / 51.8/52 / [4]
tet(B) / AAAACTTATTATATTATAGTG / TGGAGTATCAATAATATTCAC / 169 / 40/40 / [5]
qnrS / CGACGTGCTAACTTGCGTGA / GGCATTGTTGGAAACTTGCA / 118 / 54/55 / [6]
fexA / ATTCTCCCGCAAATAACG / TCGGCTCAGTAGCATCACG / 156 / 52/55 / [7]
sulI / CGCACCGGAAACATCGCTGCAC / TGAAGTTCCGCCGCAAGGCTCG / 163 / 56.3/56 / [8]
IntI1 / GGCTTCGTGATGCCTGCTT / CATTCCTGGCCGTGGTTCT / 146 / 55/55 / [9]
16S rRNA / CCTACGGGAGGCAGCAG / TTACCGCGGCTGCTGGCAC / 193 / 55/55 / [10]

aAmplicon size (bp); bMelting temperature (PCR/qPCR) (°C)

PCR procedure was as follows: initial 94 °C denaturation for 5 min, followed by 35 cycles consisting of denaturation (94 °C for 30 s), annealing for 30 s(the annealing temperature shown as above), extension (72 °C for 45 s), and a final extension step (72 °C for10 min).

Table S2

The qPCR assay performance characteristics for the target genes

Genes / Preparation of
standard curves / Efficiency
of reaction / Limit of
Detection (copies) / Correlation
Coefficients
(R2 values)
MLS resistance genes
ereA / Y=-3.092x+37.240 / 110.6% / 5 / 0.990
ermA / Y=-3.052x+37.305 / 112.7% / 6 / 0.993
ermB / Y=-2.995x+36.657 / 115.7% / 4 / 0.996
ermF / Y=-3.149x+38.009 / 107.8% / 9 / 0.998
mefA / Y=-3.191x+37.169 / 105.7% / 5 / 0.991
mphA / Y=-3.109x+38.549 / 109.7% / 14 / 0.993
vatB / Y=-3.072x+37.316 / 111.6% / 6 / 0.995
Potential indicators
intI 1 / Y=-3.668x+39.502 / 97.4% / 16 / 0.990
sul1 / Y=-3.244x+40.109 / 103.4% / 37 / 0.998
tet(B) / Y=-3.528x+40.228 / 92.1% / 30 / 0.990
fexA / Y=-3.339x+38.641 / 99.3% / 12 / 0.994
qnrS / Y=-3.042x+37.863 / 113.2% / 9 / 0.994
16S rRNA / Y=-3.189x+39.433 / 105.8% / 24 / 0.998

Table S3

Absolute concentrations of genes data in NE solid samples. (Copies/g)

Genes / Northeast pond (NE)-solid samples
Fecal-1 / Fecal-2 / Fecal-3 / Fecal-4 / Fecal-5 / Fecal-6 / Soil-1 / Soil-2 / Soil-3
ereB / 1.7×103±
6.7×102 / 5.0×102±
6.9×101 / 7.7×102±
1.3×102 / 5.3×102±
2.6×101 / 6.5×102±
4.1×101 / 4.0×102±
2.1×101 / nd / nd / nd
ermA / nd / nd / nd / nd / nd / nd / 3.6×102±
2.5×102 / 5.0×102±
3.6×101 / 4.9×102±
1.8×101
ermB / 1.5×104±
4.7×103 / 1.0×104±
2.8×103 / 3.1×104±
5.2×103 / 9.1×104±
7.0×103 / 5.2×104±
8.3×103 / 3.9×104±
3.1×103 / 8.9×102±
2.0×102 / 1.2×103±
1.9×102 / 9.3×102±
3.9×101
ermF / 2.8×104±
7.8×103 / 1.7×104±
4.8×103 / 1.4×104±
4.4×103 / 2.9×104±
1.1×103 / 6.8×103±
1.1×102 / 8.0×103±
2.7×102 / 7.2×102±
2.1×102 / 8.5×102±
4.9×101 / 9.0×102±
6.9×101
mefA / 2.0×102±
5.0×101 / 2.8×102±
5.9×101 / 3.5×102±
1.0×101 / 9.0×102±
5.0×101 / 9.2×102±
3.0×101 / 2.1×102±
3.0×101 / 2.0×102±
3×100 / 2.6×102±
1.8×100 / 1.4×102±
6.7×101
mphA / 1.9×102±
2.7×101 / 7.1×102±
6.9×101 / 8.0×102±
9.8×100 / 2.7×102±
4.8×101 / 6.5×102±
1.3×101 / 1.4×102±
6.8×101 / 4.9×102±
1.6×102 / 5.8×102±
7.9×101 / 5.5×102±
7.7×101
vatB / 8.6×103±
9.3×102 / 1.0×103±
1.3×102 / 4.7×103±
7.4×102 / 1.6×103±
2.5×102 / 9.2×103±
2.6×102 / 3.0×103±
4.8×102 / nd / nd / nd
tet(B) / 2.2×103±
6.1×102 / 1.7×103±
4.2×102 / 2.1×103±.
2.8×102 / 1.8×103±
2.0×102 / 1.8×103±
8.6×102 / 1.8×103±
1.6×102 / 3.7×102±
4.3×101 / 3.7×102±
3.8×101 / 7.1×102±
4.8×101
qnrs / 1.4×105±
7.1×104 / 5.3×105±
7.2×104 / 5.4×105±
4.5×104 / 1.8×105±
1.2×104 / 5.8×105±
1.8×104 / 6.3×105±
8.0×104 / 2.1×103±
6.9×102 / 1.9×103±
2.7×102 / 2.8×103±
3.4×102
sul1 / 1.9×105±
2.7×104 / 2.8×105±
4.7×104 / 3.0×104±
3.8×103 / 1.4×105±
3.3×104 / 2.6×105±
4.3×104 / 7.1×104±
6.8×104 / 5.7×103±
2.8×102 / 7.9×103±
2.7×102 / 8.4×103±
2.9×102
fexA / 1.5×102±
4.8×100 / 1.2×102±
1.7×101 / 1.8×101±
1.2×100 / 5.9×101±
2.0×100 / 2.0×102±
6.3×101 / 5.4×101±
3.0×100 / nd / nd / nd
intI1 / 9.3×104±
3.2×103 / 4.7×105±
1.4×104 / 9.6×104±
1.7×103 / 3.4×105±
4.3×104 / 4.3×105±
1.5×104 / 1.0×105±
5.1×104 / 1.2×105±
2.8×104 / 7.9×104±
3.7×103 / 4.8×104±
3.9×103
16s rRNA / 2.6×107±
8.8×106 / 3.3×106±
2.5×105 / 2.1×105±
5.9×104 / 1.3×106±
3.0×105 / 3.5×106±
3.4×105 / 2.1×106±
4.7×105 / 4.1×106±
3.5×105 / 4.5×106±
3.6×105 / 4.7×106±
6.9×105

nd: not detected.

Table S4

Absolute concentrations of genes data in NE water samples. (Copies/ml)

Genes / Northeast pond (NE)-water samples
Water-1 / Water-2 / Water-3 / Water-4 / Water-5
ereB / 4.2×102±
6.3×101 / 2.3×102±
4.8×101 / 7.5×102±
5.3×101 / 4.3×102±
1.0×101 / 6.5×102±
1.0×101
ermA / 3.8×102±
7.8×101 / 4.8×102±
5.2×101 / 3.9×102±
4.9×101 / 2.6×102±
4.0×101 / 1.9×102±
1.6×101
ermB / 1.7×104±
2.4×103 / 1.0×104±
3.4×103 / 1.3×104±
1.1×103 / 1.7×104±
3.7×103 / 4.6×104±
2.6×103
ermF / 5.6×104±
4.9×103 / 1.4×104±
2.3×103 / 5.7×104±
5.8×103 / 1.2×105±
1.2×104 / 1.4×105±
9.2×104
mefA / 2.4×102±
3.6×101 / 4.5×102±
2.8×101 / 5.5×102±
6.3×101 / 8.2×102±
2.9×101 / 8.3×102±
9.2×101
mphA / 4.7×103±
6.4×102 / 2.8×103±
2.2×102 / 1.8×103±
3.5×102 / 2.2×103±
2.9×102 / 4.3×103±
5.1×102
vatB / 4.8×102±
8.8×101 / 4.7×102±
1.5×101 / 5.9×102±
4.4×101 / 9.9×102±
1.4×101 / 1.1×102±
1.2×101
tet(B) / 2.9×103±
1.2×103 / 1.1×103±
4.5×103 / 2.8×103±
6.6×103 / 4.1×103±
4.0×103 / 2.3×103±
9.0×103
qnrs / 5.7×103±
7.1×102 / 4.6×103±
3.1×102 / 3.7×103±
4.8×102 / 3.9×103±
3.7×102 / 9.1×103±
6.8×102
sul1 / 2.7×106±
6.0×105 / 1.7×106±
2.6×105 / 1.9×106±
2.8×105 / 3.2×106±
5.2×105 / 2.6×106±
8.8×105
fexA / 4.6×103±
6.8×102 / 3.1×103±
6.2×102 / 2.4×103±
3.2×102 / 5.0×103±
2.8×102 / 5.1×103±
2.2×102
intI1 / 3.0×105±
1.3×104 / 2.7×105±
1.1×104 / 4.9×105±
2.9×104 / 1.8×105±
4.2×104 / 5.8×105±
1.1×104
16s rRNA / 6.1×106±
5.6×105 / 1.7×106±
2.3×105 / 1.3×107±
2.8×106 / 1.2×107±
2.2×106 / 1.2×107±
7.2×106

Table S5

Absolute concentrations of genes data in NW solid samples. (Copies/g)

Genes / Northwest pond (NW)-solid samples
Sediment-1 / Sediment-2 / Sediment-3 / Sediment-4 / Sediment-5 / Soil-1 / Soil-2 / Soil-3
ereB / 9.0×102±
2.6×101 / 6.1×102±
3.2×101 / 7.3×102±
5.1×101 / 1.8×103±
2.6×102 / 1.9×103±
3.0×102 / nd / nd / nd
ermA / nd / nd / nd / nd / nd / 1.5×103±
1.4×102 / 1.2×103±
2.4×102 / 1.8×103±
4.1×102
ermB / 7.6×103±
6.6×102 / 8.2×103±
9.9×102 / 2.2×103±
1.5×102 / 2.9×103±
1.5×102 / 5.9×103±
9.0×102 / 5.0×102±
1.3×102 / 7.2×102±
2.3×102 / 4.2×102±
9.2×102
ermF / 1.2×103±
5.9×102 / 1.7×103±
2.5×102 / 2.9×103±
7.4×102 / 2.5×103±
5.4×101 / 9.8×103±
6.0×102 / 5.3×102±
4.5×101 / 6.5×102±
6.4×101 / 5.2×102±
4.6×101
mefA / nd / nd / nd / nd / nd / nd / nd / nd
mphA / 6.9×102±
3.6×101 / 6.8×102±
3.2×101 / 4.4×102±
2.8×101 / 3.8×102±
9.0×101 / 7.3×102±
4.0×101 / 5.2×102±
1.3×102 / 4.6×102±
1.6×102 / 3.9×102±
2.8×102
vatB / 2.0×103±
5.5×102 / 4.0×103±
7.5×102 / 1.5×103±
2.2×102 / 2.2×103±
1.3×102 / 2.0×103±
3.2×102 / 1.2×102±
7.3×101 / 6.2×102±
8.9×101 / 1.7×102±
6.0×101
tet(B) / 1.6×103±
9.5×102 / 2.0×103±
5.9×102 / 2.6×103±
1.2×102 / 2.2×103±
4.9×102 / 1.8×103±
3.1×102 / 7.4×102±
1.5×101 / 1.9×102±
9.3×101 / 5.9×102±
1.3×101
qnrs / 1.6×104±
1.2×103 / 4.4×104±
1.5×103 / 1.8×104±
1.3×103 / 3.6×104±
3.8×103 / 1.3×104±
6.0×103 / 2.1×103±
3.8×102 / 2.2×103±
7.8×102 / 1.8×103±
3.8×102
sul1 / 2.7×105±
2.7×104 / 1.7×105±
4.6×104 / 2.4×105±
4.8×104 / 8.6×105±
1.6×104 / 1.3×105±
6.8×104 / 4.6×102±
1.3×102 / 2.2×103±
7.9×102 / 7.5×102±
8.2×101
fexA / nd / nd / nd / nd / nd / 3.6×103±
3.9×102 / 4.1×103±
1.1×102 / 1.7×103±
6.5×102
intI1 / 5.8×105±
3.2×105 / 5.7×105±
2.2×105 / 6.3×105±
2.0×105 / 5.1×105±
2.9×105 / 3.3×105±
8.0×105 / 8.5×104±
3.9×103 / 2.1×104±
7.0×103 / 3.0×104±
5.8×103
16s rRNA / 8.9×106±
7.4×105 / 7.6×106±
8.3×105 / 3.8×106±
2.5×105 / 2.3×106±
6.4×105 / 7.2×106±
4.2×105 / 3.6×106±
3.1×105 / 5.4×106±
6.5×105 / 4.6×106±
1.3×105

nd: not detected.

Table S6

Absolute concentrations of genes data in NW water samples. (Copies/ml)

Genes / Northwast pond (NW)-water samples
Water-1 / Water-2 / Water-3 / Water-4 / Water-5
ereB / 3.6×103±
8.1×102 / 1.5×103±
8.8×102 / 8.1×103±
1.8×102 / 3.4×103±
2.6×102 / 1.7×103±
2.2×102
ermA / 7.8×102±
1.1×102 / 1.1×103±
5.5×102 / 1.3×103±
1.8×102 / 1.9×102±
2.6×102 / 5.9×102±
5.3×102
ermB / 3.3×104±
3.6×103 / 1.0×104±
1.6×103 / 1.2×104±
2.2×103 / 1.1×104±
6.1×103 / 1.6×104±
1.5×103
ermF / 2.5×104±
2.2×103 / 2.0×104±
2.7×103 / 6.0×104±
1.2×103 / 5.4×104±
4.7×103 / 1.4×104±
1.9×103
mefA / 1.4×103±
9.2×102 / 4.5×103±
8.8×102 / 5.0×103±
2.8×102 / 2.6×103±
8.2×102 / 5.5×103±
5.9×102
mphA / 1.5×104±
4.1×103 / 5.7×103±
9.6×102 / 2.9×103±
5.7×102 / 2.5×103±
1.0×102 / 6.0×103±
3.7×102
vatB / 9.3×103±
2.7×102 / 4.1×103±
8.5×102 / 1.2×103±
5.0×102 / 1.2×103±
1.1×102 / 2.3×103±
2.6×102
tet(B) / 3.5×104±
3.1×103 / 1.1×104±
1.7×103 / 3.0×104±
1.9×103 / 1.7×104±
4.3×103 / 1.0×104±
3.2×103
qnrs / 2.5×104±
7.1×103 / 4.3×104±
3.9×103 / 1.8×104±
7.6×103 / 5.6×104±
8.4×103 / 3.1×104±
6.8×103
sul1 / 3.2×106±
2.7×105 / 2.2×106±
5.4×105 / 1.0×106±
1.9×105 / 1.6×106±
5.0×105 / 2.5×106±
3.7×105
fexA / 5.0×103±
5.0×102 / 4.3×103±
4.9×102 / 1.1×103±
9.7×102 / 1.9×103±
2.6×102 / 6.5×103±
1.6×102
intI1 / 1.6×105±
1.1×104 / 5.1×105±
6.0×104 / 6.7×105±
2.7×104 / 2.0×105±
2.5×104 / 5.8×105±
7.6×104
16s rRNA / 3.3×106±
4.4×105 / 5.4×106±
3.9×105 / 3.9×106±
6.0×105 / 7.2×106±
1.7×105 / 2.6×107±
2.3×106

Table S7

Absolute concentrations of genes data in S solid samples. (Copies/g)

Genes / South pond (S)-solid samples
Fecal-1 / Fecal-2 / Fecal-3 / Fecal-4 / Fecal-5 / Fecal-6 / Fecal-7 / Soil-1 / Soil-2 / Soil-3
ereB / 5.5×102±
2.4×101 / 3.5×102±
7.6×101 / 1.6×102±
2.5×101 / 3.4×102±
5.8×101 / 2.0×102±
3.4×101 / 6.3×102±
1.1×101 / 3.1×102±
9.0×100 / 1.6×102±
2.4×101 / 1.1×102±
6.4×101 / 1.6×102±
2.4×101
ermA / 1.7×102±
1.9×101 / 1.3×102±
5.9×101 / 3.9×102±
6.2×101 / 4.2×102±
7.8×101 / 3.2×102±
1.1×101 / 7.0×102±
5.0×101 / 4.7×102±
1.9×101 / nd / nd / nd
ermB / 5.5×104±
1.6×103 / 5.8×104±
1.4×103 / 4.1×104±
1.1×103 / 5.1×104±
3.5×103 / 3.8×104±
2.6×103 / 3.4×104±
1.6×103 / 1.3×104±
1.4×103 / 3.2×103±
3.8×102 / 9.8×103±
1.0×102 / 3.2×103±
3.8×102
ermF / 3.2×104±
6.2×103 / 5.6×104±
4.2×103 / 1.3×104±
3.8×103 / 6.7×104±
8.7×103 / 1.8×104±
1.7×103 / 6.6×104±
3.6×103 / 1.2×104±
1.8×103 / 3.2×102±
2.7×101 / 5.3×102±
7.2×101 / 1.1×103±
1.5×102
mefA / 3.8×102±
5.0×101 / 1.9×102±
6.8×101 / 2.8×102±
5.7×101 / 3.8×102±
8.7×101 / 2.9×102±
7.9×101 / 7.2×102±
8.0×101 / 1.5×102±
7.1×101 / 5.0×103±
7.1×102 / 5.9×103±
8.5×102 / 1.0×104±
8.3×103
mphA / 2.5×103±
7.0×102 / 8.4×103±
7.0×102 / 3.1×103±
3.7×102 / 2.2×103±
8.0×101 / 1.2×103±
2.0×102 / 1.8×103±
1.0×102 / 6.2×103±
1.6×102 / nd / nd / nd
vatB / 1.7×104±
1.0×103 / 2.6×104±
2.7×103 / 9.1×103±
6.2×102 / 5.2×104±
6.3×103 / 2.5×104±
6.3×103 / 8.6×104±
3.5×103 / 7.8×104±
1.9×103 / nd / nd / nd
tet(B) / 2.5×103±
4.5×102 / 1.6×103±
3.1×102 / 1.5×103±
5.8×102 / 1.8×103±
5.0×102 / 5.4×103±
2.7×102 / 2.4×103±
2.2×102 / 2.2×103±
8.1×102 / 2.2×103±
3.4×102 / 1.7×103±
9.1×102 / 2.6×103±
2.7×102
qnrs / 2.9×102±
7.1×101 / 2.8×102±
9.0×101 / 6.2×102±
1.4×101 / 6.0×102±
8.1×101 / 3.4×102±
2.2×101 / 4.1×102±
1.1×101 / 4.8×102±
1.0×101 / nd / nd / nd
sul1 / 3.2×104±
3.2×103 / 2.3×104±
1.3×103 / 5.1×104±
7.7×103 / 8.5×104±
1.7×103 / 6.0×104±
1.8×103 / 3.2×104±
3.2×103 / 3.2×105±
5.5×104 / 4.2×102±
4.4×101 / 8.0×102±
9.3×101 / 5.2×102±
9.3×101
fexA / 9.2×102±
4.8×101 / 6.6×102±
2.8×101 / 7.4×102±
5.7×101 / 1.5×102±
5.0×101 / 4.7×102±
8.8×101 / 7.1×102±
1.1×101 / 9.6×102±
1.7×101 / nd / nd / nd
intI1 / 4.4×104±
2.5×103 / 2.6×105±
1.4×104 / 2.4×105±
4.2×104 / 2.8×105±
4.2×104 / 4.9×104±
1.4×103 / 4.6×104±
1.9×103 / 1.3×105±
1.1×104 / 1.0×104±
4.3×103 / 2.0×104±
1.3×103 / 3.6×104±
8.5×103
16s rRNA / 3.2×106±
2.4×105 / 3.0×106±
1.1×105 / 4.5×106±
2.3×105 / 1.0×106±
2.4×105 / 4.1×106±
7.8×105 / 1.9×106±
2.8×105 / 1.5×106±
2.2×105 / 3.0×106±
1.3×105 / 3.9×106±
9.5×105 / 1.8×106±
1.2×105

nd: not detected.

Table S8

Absolute concentrantions of genes data in S solid samples. (Copies/g)

Genes / South pond (S)-solid samples
Sediment-1 / Sediment-2 / Sediment-3 / Sediment-4 / Sediment-5 / Sediment-6
ereB / 1.1×102±
1.4×101 / 4.2×102±
3.9×101 / 1.5×102±
4.2×101 / 1.3×102±
5.3×101 / 8.6×102±
5.9×101 / 3.4×102±
2.6×101
ermA / nd / nd / nd / nd / nd / nd
ermB / 1.2×104±
2.1×103 / 1.6×104±
2.3×103 / 3.1×104±
5.2×103 / 6.0×104±
5.1×103 / 4.9×104±
3.7×103 / 5.6×104±
4.8×103
ermF / 1.1×103±
2.4×102 / 8.3×103±
7.0×102 / 5.5×103±
5.1×102 / 1.1×103±
2.4×102 / 8.9×102±
2.4×102 / 9.5×103±
3.8×102
mefA / nd / nd / nd / nd / nd / nd
mphA / 5.0×102±
3.7×101 / 1.4×102±
3.2×101 / 3.8×102±
3.2×101 / 2.5×102±
4.3×101 / 8.8×102±
1.8×101 / 5.8×102±
7.3×101
tet(B) / 3.5×102±
1.0×101 / 9.1×102±
1.7×101 / 5.1×102±
1.3×101 / 3.0×102±
1.4×101 / 6.3×102±
1.8×101 / 4.8×102±
7.2×101
tetB / 3.0×104±
5.3×103 / 5.9×104±
2.4×103 / 2.1×104±
1.1×103 / 4.6×104±
4.3×103 / 9.3×104±
8.3×103 / 8.9×104±
3.8×103
qnrs / 7.4×104±
3.8×103 / 1.7×104±
2.2×103 / 6.2×104±
1.5×103 / 2.4×104±
6.8×103 / 5.7×104±
4.8×103 / 3.9×104±
5.7×103
sul1 / 3.2×104±
3.2×103 / 3.2×104±
3.8×103 / 1.2×104±
1.1×103 / 4.7×104±
3.8×103 / 4.7×104±
8.2×103 / 2.9×104±
4.8×103
fexA / nd / nd / nd / nd / nd / nd
intI1 / 2.7×105±
3.0×104 / 4.9×105±
3.0×104 / 2.1×105±
1.2×104 / 1.9×105±
5.9×104 / 2.1×105±
4.7×104 / 1.9×105±
2.3×104
16s rRNA / 2.1×106±
4.5×105 / 3.2×106±
5.3×105 / 1.1×106±
1.9×105 / 2.8×106±
4.4×105 / 4.9×106±
6.3×105 / 2.6×106±
4.9×105

nd: not detected.

Table S9

Absolute concentrantions of genes data in S water samples. (Copies/ml)

Genes / South pond (S)-water samples
Water-1 / Water-2 / Water-3 / Water-4 / Water-5
ereB / 5.1×103±
6.4×102 / 1.3×103±
4.7×102 / 1.3×103±
3.3×102 / 1.1×103±
7.1×102 / 1.3×103±
5.2×102
ermA / 1.6×104±
2.1×103 / 2.1×104±
3.3×103 / 1.7×104±
3.0×103 / 2.7×104±
3.8×103 / 2.7×104±
4.7×103
ermB / 2.6×104±
3.1×103 / 2.0×104±
3.4×103 / 2.9×104±
3.4×103 / 1.6×104±
2.8×103 / 6.1×104±
6.5×103
ermF / 5.4×104±
2.7×103 / 8.2×104±
8.1×103 / 1.1×104±
1.9×103 / 1.4×104±
1.8×103 / 4.7×104±
3.2×103
mefA / 2.3×103±
4.2×102 / 1.7×103±
6.2×102 / 9.2×103±
7.3×102 / 9.4×103±
5.8×102 / 2.3×103±
6.9×102
mphA / 3.8×104±
4.2×103 / 1.8×104±
9.4×103 / 1.6×104±
1.6×103 / 1.7×104±
1.8×103 / 2.0×104±
2.5×103
vatB / 4.3×103±
5.8×102 / 7.5×103±
2.1×102 / 1.5×103±
5.0×102 / 8.4×103±
9.7×102 / 3.4×103±
3.6×102
tet(B) / 6.5×104±
8.2×103 / 1.7×104±
4.1×103 / 1.1×104±
1.1×103 / 8.9×104±
1.6×103 / 9.5×104±
2.6×103
qnrs / 4.4×104±
6.1×103 / 2.2×104±
4.9×103 / 2.9×104±
3.9×103 / 3.8×104±
5.8×103 / 7.2×104±
8.3×103
sul1 / 1.1×106±
5.0×105 / 1.9×106±
1.2×105 / 1.7×106±
6.5×105 / 1.8×106±
2.3×105 / 1.2×106±
2.4×105
fexA / 9.9×103±
3.5×103 / 4.8×103±
6.9×103 / 1.8×103±
1.8×103 / 3.8×103±
1.6×103 / 5.1×103±
6.8×103
intI1 / 3.7×105±
1.9×104 / 5.0×105±
2.1×104 / 6.1×105±
1.8×104 / 3.5×105±
3.2×104 / 3.4×105±
1.9×104
16s rRNA / 4.5×106±
1.2×105 / 2.3×106±
2.7×105 / 2.0×106±
5.1×105 / 7.6×106±
3.4×105 / 2.6×106±
5.3×105

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