Supplemental File S1. Data on Peptide Mass Fingerprint
Mascot Search Results
Database : MSDB 20060529x (3038380 sequences; 1012707669 residues)
Taxonomy : Viridiplantae (Green Plants) (222498 sequences)
Top Score : 66 for Q9SFU3_ARATH, Putative purple acid phosphatase.- Arabidopsis thaliana (Mouse-ear cress).
Probability Based Mowse Score
Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 66 are significant (p<0.05).
1. / Q9SFU3_ARATHMass:60396Score:66Expect:0.053Queries matched:10
Putative purple acid phosphatase.- Arabidopsis thaliana (Mouse-ear cress).
Search Parameters
Type of search : Peptide Mass Fingerprint
Enzyme : Trypsin
Mass values : Monoisotopic
Protein Mass : Unrestricted
Peptide Mass Tolerance : ± 0.18 Da
Peptide Charge State : 1+
Max Missed Cleavages : 1
Number of queries : 58
Protein View
Match to: Q9SFU3_ARATH Score: 66 Expect: 0.053
Putative purple acid phosphatase.- Arabidopsis thaliana (Mouse-ear cress).
Nominal mass (Mr): 60396; Calculated pI value: 5.71
NCBI BLAST search of Q9SFU3_ARATH against nr
Unformatted sequence string for pasting into other applications
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 58
Number of mass values matched: 10
Sequence Coverage: 25%
Matched peptides shown in Bold Red
1 MTFLLLLLFC FLSPAISSAH SIPSTLDGPF VPVTVPLDTS LRGQAIDLPD
51 TDPRVRRRVI GFEPEQISLS LSSDHDSIWV SWITGEFQIG KKVKPLDPTS
101 INSVVQFGTL RHSLSHEAKG HSLVYSQLYP FDGLLNYTSG IIHHVRITGL
151 KPSTIYYYRC GDPSRRAMSK IHHFRTMPVS SPSSYPGRIA VVGDLGLTYN
201 TTDTISHLIH NSPDLILLIG DVSYANLYLT NGTSSDCYSC SFPETPIHET
251 YQPRWDYWGR FMENLTSKVP LMVIEGNHEI ELQAENKTFE AYSSRFAFPF
301 NESGSSSTLY YSFNAGGIHF VMLGAYIAYD KSAEQYEWLK KDLAKVDRSV
351 TPWLVASWHP PWYSSYTAHY REAECMKEAM EELLYSYGTD IVFNGHVHAY
401 ERSNRVYNYE LDPCGPVYIV IGDGGNREKM AIEHADDPGK CPEPLTTPDP
451 VMGGFCAWNF TPSDKFCWDR QPDYSALRES SFGHGILEMK NETWALWTWY
501 RNQDSSSEVG DQIYIVRQPD RCPLHHRLVN HC
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence
43 - 54 1297.6400 1296.6327 1296.6310 0.0018 0 R.GQAIDLPDTDPR.V
92 - 111 2199.1729 2198.1656 2198.2422 -0.0766 1 K.KVKPLDPTSINSVVQFGTLR.H
93 - 111 2071.1041 2070.0968 2070.1473 -0.0505 0 K.VKPLDPTSINSVVQFGTLR.H
147 - 159 1574.8474 1573.8401 1573.8504 -0.0103 0 R.ITGLKPSTIYYYR.C
255 - 260 882.4099 881.4027 881.3820 0.0206 0 R.WDYWGR.F
255 - 260 882.5559 881.5486 881.3820 0.1666 0 R.WDYWGR.F
269 - 287 2162.9997 2161.9925 2162.1041 -0.1116 0 K.VPLMVIEGNHEIELQAENK.T
349 - 371 2791.2160 2790.2088 2790.3343 -0.1255 0 R.SVTPWLVASWHPPWYSSYTAHYR.E
378 - 402 2943.2627 2942.2554 2942.3544 -0.0990 0 K.EAMEELLYSYGTDIVFNGHVHAYER.S
502 - 517 1809.8303 1808.8230 1808.8540 -0.0310 0 R.NQDSSSEVGDQIYIVR.Q
>P1;Q9SFU3_ARATH
Putative purple acid phosphatase.- Arabidopsis thaliana (Mouse-ear cress).
C;Species Q9SFU3_ARATH: Arabidopsis thaliana (Mouse-ear cress).
C;Species AAF20233: Arabidopsis thaliana
C;Species AAN74650: Arabidopsis thaliana
C;Family: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
C;Accession: Q9SFU3;
C;Gene name: Name=T1B9.21; OrderedLocusNames=At3g07130;
C;Reference [1]
C;NUCLEOTIDE SEQUENCE.
R;Lin X., Kaul S., Town C.D., Benito M.-I., Creasy T.H., Haas B., Ronning C.M., Koo H., Fujii C.Y., Utterback T.R., Barnstead M.E., Bowman C.L., White O., Nierman W.C., Fraser C.M.;
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
C;Reference [2]
C;NUCLEOTIDE SEQUENCE.
C;PubMed=16244908; DOI=10.1007/s11103-005-0183-0;
R;Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.;
Plant Mol. Biol. 59:581-594(2005).
C;01-MAY-2000, integrated into UniProtKB/TrEMBL.
C;01-MAY-2000, sequence version 1.
C;18-APR-2006, entry version 26.
C;TAIR; At3g07130; -.