Supplemental Figure S1. Phylogenic Relationship of Ricenac Family

Supplemental Figure S1. Phylogenic Relationship of RiceNAC family.

A phylogenic dendrogram was derived from an alignment of the deducedamino acid sequences of 87 rice NAC domains using the MEGA program. Of 140 NAC factors predicted from the rice genome, we selected eighteen that have EST information from NCBI database search using the tBLAST N program. Eighteen stress-inducible factors were indicated by boxes. Our subgroups I to III shown in Table I are marked by roman letter. The numbers at the branches are confidence values (B = 1000 bootstrap replications).

Supplemental Figure S2. Regulated Genes in the Roots and Leaves of RCc3:OsNAC10and GOS2:OsNAC10Transgenic Rice Plants under Normal and Stress Conditions.

Transcript levels of OsNAC10 and eight target genes,Zn-finger (Os11g0702400), HAK5(Os03g0575200), 2OG-Fe (II) (Os04g0581100), NCED(Os07g0154100), NAC (Os11g0154500), KUP3 (Os09g0448200), MutS4 (Os07g0486000) and Peroxidase (Os12g0448900)inroots and leaves of RCc3:OsNAC10,GOS2:OsNAC10 and NT plants both before and after stress treatments were measured by qRT-PCR. Dip1 was used as a marker for up-regulation of key genes following stress treatments. Data were normalized using the rice ubiquitin gene (OsUbi) transcript levels. Values are the means ± SD of three independent experiments. The panel at the bottom describes tissue specificity of OsNAC10-dependent expression of target genes in unstressed transgenic plants (Supplemental Tables S1and S2).

Supplemental Table S1. Up-regulated Root Expressed Genes in eitherRCc3:OsNAC10 or GOS2:OsNAC10 Plants in Comparison to Non-transgenic Controls.

aSequence identification numbers for the full-length cDNA sequences of the corresponding genes. bThe mean of three independent biological replicates.Numbers in boldface indicate up-regulation by more than 3-fold (P<0.05).These microarray data sets can be found at (Gene Expression Omnibus, GEO).cP values were analyzed by one-way ANOVA. Bold letters in gene names indicate ones that were confirmed by qPCR (Fig. 4; Supplemental Fig. S2).

SupplementalTable S1. (Continued)

SupplementalTable S1. (Continued)

SupplementalTable S1. (Continued)

Supplemental Table S2. Up-regulated Leaf Expressed Genes in GOS2:OsNAC10 Plants in Comparison to Non-transgenic Controls.

aSequence identification numbers for the full-length cDNA sequences of the corresponding genes. bThe mean of three independent biological replicates.Numbers in boldface indicate up-regulation by more than 3-fold (P<0.05).These microarray data sets can be found at (Gene Expression Omnibus, GEO).cP values were analyzed by one-way ANOVA. Bold letters in gene names indicate ones that were confirmed by qPCR (Fig. 4; Supplemental Fig. S2).

SupplementalTable S2. (Continued)

Supplemental Table S3. Agronomic Traits of the RCc3:OsNAC10 and GOS2:OsNAC10Transgenic Rice Plants under Normal Field Conditions

*The mean difference is significant at the 0.05 level (LSD).

Supplemental Table S4. Agronomic Traits of the RCc3:OsNAC10 and GOS2:OsNAC10Transgenic Rice Plants under Field Drought Conditions

*The mean difference is significant at the 0.05 level (LSD).

Supplemental Table S5. Primer list for PCR