Supplementary Data

Supplemental Figure 1.Photomorphogenic phenotypesof the brm-3 and brm-20 mutants.

(A) Phenotypic images of Col and brm mutants grown under continuous light conditions for 5 d. Bar, 2 mm.

(B) Hypocotyl lengths of seedlings as shown in (A). Mean ±SD, n=20.

Supplemental Figure2.Endogenous BRM transcript levels in the BRM-RNAitransgenic lines.

Seedlings were grown in darkness for 5 d. The expression levels of BRMwere normalized to those of UBQ10. Mean ±SD, n=3.

Supplemental Figure3.Relative expression of chlorophyll biosynthetic genes.

Seedlings were grown in darkness for 5 d. The expression levels were normalized to those of UBQ10. Mean ±SD, n=3.

Supplemental Figure 4. Relative enrichment of PORC fragment by ChIP assays.

35S:PIF1-LUC seedlings were grown in the dark for 5 d and then exposed to white light for an additional 3 or 6 h. The anti-BRM and anti-LUC antibodies were used to precipitate the protein-DNA complexes and precipitation with serum was used as a control. Enrichment of the “c” fragment of PORC (shown in Fig. 6A) was analyzed by qPCR. Mean ±SD, n=3.

Supplementary Table 1. Primers used in this study.

Gene / Oligo name / Sequence (5’-3’) / Purpose
BRM / BRM-mfe1-F
BRM-N-R / CAATTGATGCAATCTGGAGGCAGTGGCGGAG
GTCGACAAGCATTGAAGGTTGTCTTACAACC / Constructing BRM-N
BRM-ATP-F
BRM-ATP-R / GAATTCATGCAGGCTGGAACTTTGCGTGATTAC
GTCGACCCCTATACGATGGGCTCTGGCAACTG / Constructing BRM-M
BRM-C-F
BRM-sal1-R / CAATTGCAGACAAGGGAAGTAAAAGTG
GTCGACTAAATGGCTAGGCCGTCTTTTACCAG / Constructing BRM-C
BRM-AG-F
BRM-AG-R / CAATTGCCAATGCAGCAAGCGTATATTC
GTCGACATTTCCAGGCATTAGTTGTCCGG / Constructing GST-BRM-AG
BRM-pro-F1
BRM-pro-R1 / GAGCTCCAGGTCTTCTAAACTCTTGCTTC
GTCGACCAATTGGTCGAAGAATCTGACAGTCACTAC / Constructing ProBRM:GUS
BRM-RNAi-F
BRM-RNAi-R / ACTAGTGGTACCGCCAGAAGCGAGGAGGAAGTTGAG
GAGCTCGGATCCCGGAGGATAATCATAACCACAGAC / Constructing BRM-RNAi
BRM-RNAi-F
BRM-Q-R / ACTAGTGGTACCGCCAGAAGCGAGGAGGAAGTTGAG
GATGTGGGACATTCTTGCAC / Characterization of brm-3
BRM-Q-F
BRM-Q-R / GCTACACAGCTTACCGTGGA
GATGTGGGACATTCTTGCAC / qRT-PCR
LUC / LUC-F
LUC-R / AAGCTTAGGCCTGGGCCCACTAGTACCATGGAAGACGCCAAAAACATAAAG
CACGTGATTTAAATTTACACGGCGATCTTTCCGCCC / Constructing pCAMBIA1302-35S-LUC
35S Pro / 35Sp-F
35Sp-R / GAATTCGGCACTGGCCGTCGTTTTACA
GAGCTCATGGTCAAGAGTCCCCCGTGTTC
PIF1 / PIF1-F
PIF1-R / CTGCAGACCATGGATATGCATCATTTTGTCCCTGAC
ACTAGTACCTGTTGTGTGGTTTCCGTG / Constructing 35S:PIF1-LUC
PORC / PORCRealTf
PORCRealTr / ACTGGAGCTTCGTCTGGTTT
AGAAAGTTCCTGCAAGCCATG / qRT-PCR
PORC-CHIP1-F
PORC-CHIP1-R / TGCATATTTCTATGGTGTGTCATT
GATGGCAGCGGTCAAGTAG / ChIP
PORC-CHIP2-F
PORC-CHIP2-R / GATCAAATCAAATTTCACAAGCA
TCGTCACGGTTTCTAGACCA
PORC-CHIP3-F
PORC-CHIP3-R / ATCTCAAGGCCCAATAAACG
GAATTGAGGAGCGACAGAAGA
PORA / PORARealTf
PORARealTr / TCTCCTCTGCTTTCTCTGTC
GGATCCAACAATGATGAGACG / qRT-PCR
PORA-CHIP1-F
PORA-CHIP1-R / GGTGCATTGGTTGGTTCATA
TTTCCTGTTCTTCAGATTGATTTC / ChIP
PORA-CHIP2-F
PORA-CHIP2-R / GATTTCGCAACACGAATAGC
CACATTAGAGTCTTCGCTTGCT
PORA-CHIP3-F
PORA-CHIP3-R / GGGACCACCTTTCTCTTGTC
GGGCCATTGTTTCGTTTAAG
PORB / PORBRealTf
PORBRealTr / CAAAGATGCGAAGTTGAATGC
GATTCGCCTTCGGTGGTACA / qRT-PCR
PORB-CHIP1-F
PORB-CHIP1-R / GTTGTGCAGGACACTGAGGT
TGAGAGGCTTTCCACAACAG / ChIP
PORB-CHIP2-F
PORB-CHIP2-R / AAGAGTGGATGTAATTTGACGATT
ATGGACGTTCTCATGTTTGG
PORB-CHIP3-F
PORB-CHIP3-R / CACACATCTTCGGTGACCTC
GCATCTTTGCGAACAGAGAA
UBQ10 / UBQ10-CHIP-F
UBQ10-CHIP-R / TCCAGGACAAGGAGGTATTCCTCCG
CCACCAAAGTTTTACATGAAACGAA / ChIP
HEMA1 / HEMA1RealTf
HEMA1RealTr / GTTGCTGCCAACAAAGAAGA
AATCCCTCCATGCTTCAAAC / qRT-PCR
HEMA2 / HEMA2 RealTf
HEMA2 RealTr / GCTCCTGATCCAAACCATTT
GATCAGGAATCGAGCAGACA
GSA1 / GSA1RealTf GSA1RealTr / AATGACTGCAGGGATTCACA
CCGTTGGTTAGTTCCTTCGT
GSA2 / GSA2RealTf
GSA2RealTr / CGGTTTCGGTAGAGGAGAAG
TGGTTGTCCACCAACAGATT
HEMB1 / HEMB1RealTf
HEMB1RealTr / AGGCTGAAGGAGCAGACATT
AACTTGGTATGCAGCAATCG
HEMB2 / HEMB2RealTf
HEMB2RealTr / AGAGAGCTGCCTTCCAAGAG
GTCTCCAGCCAAGCATGTAA
HEMC / HEMCRealTf
HEMCRealTr / TAGCCTCACTGAACCACGAG
TGCGTATCCAGCAATAGGAG
HEMD / HEMDRealTf
HEMDRealTr / GGTGCAGAGTGTTGATACGG
GCAACGTAATTGCTCCATTG
HEME1 / HEME1RealTf
HEME1RealTr / TGAGCGAATGAAAGGAACTG
CAGGAAGCGGAGAGAATAGG
HEME2 / HEME2RealTf
HEME2RealTr / AGACCGGCTAGGAAGAGACA
TGAATCCGGCTTGTGATAAA
HEMF1 / HEMF1RealTf
HEMF1RealTr / TAGGGCTGCTCAAGACAGTG
CTCAAAGACATTCCCGTCCT
HEMF2 / HEMF2RealTf
HEMF2RealTr / TTCCGGAAACAGAAAGACCT
TTCTTCGACTGCTTCAATGG
HEMF3 / HEMF3RealTf
HEMF3RealTr / GACGCCTTCTCTTGTTCCTC
CCTTCAGCTTCTGACCATCA
UPM1 / UPM1RealTf
UPM1RealTr / TTGTGGACCAGGAGATGTGT
GACAAGCCTGTCGTAAAGCA
HEMG1 / HEMG1RealTf
HEMG1RealTr / ACCGGAATTCTGTCCAAGTC
TGAGGCCATACCCTAACTCC
HEMG2 / HEMG2RealTf
HEMG2RealTr / ATTCCCGTTGTATGACAGCA
CCCTCGATGATTACCTGCAT
CHLI1 / CHLI1RealTf
CHLI1RealTr / GGAATCCAAATAAGGCCAAA
AAATGGATAAACCGGTCTCG
CHLI2 / CHLI2RealTf
CHLI2RealTr / CATCCTGCTCGGTTTATCCT
GCGTCTCTAACCGTCCCTAC
CHLH / CHLHRealTf
CHLHRealTr / CAGCCAACATCAGTCTTGCT
ACCTGCTTCTTCTCAGCCAT
CHLD / CHLDRealTf
CHLDRealTr / GTGGTGGTGGTTCTCCTCTT
TCGGTTATCGCAACAATCAT
CHLM / CHLMRealTf
CHLMRealTr / GCTATGGTTGCTGAAGCTGA
GCGGGTAATGTATCAACACG
CRD1 / CRD1RealTf
CRD1RealTr / AGAGTTCGAGGCTCTGCTTC
CAACGAAGATCTGTCGGAGA
CAO / CAORealTf
CAORealTr / TGAACGAGGGACGTATTCAA
GGCCAAATCCAGATCATACC
FC1 / FC1RealTf
FC1RealTr / CTCTACAACGGGTTCAAGCA
CTTCTCAATGAGGTCAGCCA
FC2 / FC2RealTf
FC2RealTr / TTGCATATGTCGAAGAAGCC
CTGGTCCAACTCTGCTCTGA
HO2 / HO2RealTf
HO2RealTr / CTTTCCCATTCCAAATCTCAA
TCTTCCTCTGTGAGGCCTTT
CAB3 / CAB3RealTf
CAB3RealTr / AGGAGCTCAAGAACGGAAGATTGG
AACTTGCTCACTTTCCGGGAACA
UBQ10 / UBQ10-RealTf
UBQ10-RealTr / TTCCTTGATGATGCTTGCTC
TTGACAGCTCTTGGGTGAAG

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