Supplemental Figure 1 Input Sequences for Mega6 Alignment and Phylogenetic Tree Construction

Supplemental Figure 1 Input sequences for Mega6 alignment and phylogenetic tree construction. The NCBI protein RefSeq database (posted date march 5 2015) was searched withPBlast (Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402) with the actin sequence from Aspergillusniger (Actin 1: XP_001396595.1) using Bacteria (taxid 2) and cyanobacteria (taxid 1117). Only proteins with coverage 80% or higher were retrieved. One MreB duplicate protein sequence of Leptotrichiahofstadii was removed. The sequences were used for alignment (Mega6, supplemental figure 2) and tree construction (Figure 5 main text)

>gi|145250163|ref|XP_001396595.1| actin [Aspergillusniger CBS 513.88]

MEEEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMIGMGQKDSYVGDEAQSKRGILTLRYPIEHGVVTNWD

DMEKIWHHTFYNELRVAPEEHPVLLTEAPINPKSNREKMTQIVFETFNAPAFYVSIQAVLSLYASGRTTGIVLDSGDGVT

HVVPIYEGFALPHAISRVDMAGRDLTDYLMKILAERGYTFSTTAEREIVRDIKEKLCYVALDFEQEIQTASQSSSLEKSY

ELPDGQVITIGNERFRAPEALFAPSVLGLESGGIHETTFNSIMKCDVDVRKDLYGNIVMSGGTTMYPGISDRMQKEITAL

APSSMKVKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

>gi|516023977|ref|WP_017454560.1| hypothetical protein [Herbaspirillumrubrisubalbicans]

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITN

WDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDAGDG

VTHNVPVYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK

SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETAYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEIT

ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

>gi|754134005|ref|WP_041765367.1| actin, cytoplasmic 2 [Leptolyngbya sp. PCC 7376]

MVIDIGSGIVKVGFAGEEAPRETFPAIVGRPRHQGVMVGMGQKDYYVGDEAQSKRGILTLKYPNEHGIVTNWDDYEKLLE

YAFKELEVDPADHPVLLTEAPLNPKANREKLTQIMFEKFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG

YALPHAIIRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVASDFEQEMQTAASSSSLEKSYTLPDGQVI

TIGNERFRCPETLFQPAFIGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMFSGIADRMQKEISALAPPTMKIK

IIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

>gi|488833380|ref|WP_002745786.1| actin, cytoplasmic 2 [Microcystisaeruginosa

MSEIVIDCGSGFIKAGFAGESKPRVVFPSVVGRPRHQGVRVDKDSYVGVEAQEKRDALKLQYPVEHGIVTYWDDFEKILD

HIFYKALRVAPEEHLIFITEPPLNPKANREKLTQTLFETFNAPGMYLGIGSVMAVYAGGRTTGLVADLGAGVTHTVPVYE

GYAIPHAVIRLDLAGEDLTEYLMKLLSQRGYSLNSFDDREMVQDLKEKLCYVALNFEEEMQTTAGREQSYTLNDGREIRI

GDECSRCAETLFQPAFIGMESAGIHETIYNSIMKCDAEIRNELFANIVLSGGTSMFPGFAERIQKEINALAPQNTQVNVI

AVPERQYSTWIGGAMLAASDDFEAMCISKQDYDENGPSIVHKKCF

>gi|759587159|ref|WP_043305783.1| hypothetical protein [Pseudomonas viridiflava

MDDKINGKPVVIDMGSAITKIGFVGEKTPRAMIPTVIGKPKSGANQTTVYVGEEAITKRESLNLSYPMSLGLVTKWDDFE

KFIHHAFNDILKISLENHPTLLTEPPLNPKANREKMTQVMFETFNCPAFYTSSNAVLSLYATGRTTGFVLDVGDSVICAA

PIYEGYVLPHATLKLNTGGRHLTETLAERLSKSSLDSIALNERISVARYVKEKLSCAALTADRVAQTGVGLFEKTYSLPD

HEDILVAKDFHCAEALFRPSLAGEGSGGIQNLIHQSITKCDADIQKDLWSNIVLSGGSAAFEKLGNRLQTELSALVPTTT

EVKVTAPIECPYLSWLGGSVLTDNPTFQEMWITRHEYDESGPSIVHRKCF

>gi|296775765|gb|ADH43034.1| Actin [uncultured SAR11 cluster alpha proteobacterium H17925_45G17

MEAALNMDVARLSGMFTETDSNVIADIELMLPDGEIIITDVPADADGRDLLEHLHGVANFRGHVLFGGQRVNEDDLVSDL

GLQVGGRLQVSANRDDEVVVMDNGSDTIKAGFGGDDTPCVIEQSVVGYDKQWRGRPQEGTPSFVGTEAFERHSTNLLKRP

IEHGMVTDWENMEVLWRHVFENSLGVSSCSGALMTEPPLNPKDNRERMTQMMFESFHLPQFYLALTPVLALYASGRTSGL

SLDSGYAVTHCVPIYEGYSLPHAIQRNDRNSGRDLTDFLSQLLPESGIRLSTSAERLIVRDMKEKLCRVSLDFDHEVNTA

MSQEYTMPDSTVVTVNNQMIRVPEVMFKPYLDGSSRRHFSIHEILKRTIFDNDIDLHRVLMENIVLSGGNTKFVNYGERM

EAEVKQLVPNPMMSSWVHTVVPRENDSLFSAWTGGALLSALSTFEGMWLTMEMYNEKGPRAIHEVCFS

>gi|655447440|ref|WP_028831144.1| hypothetical protein [Proteobacteria bacterium JGI 0000113-E04]

MSYYPSAEGESIVIDVGTGIVKAGFAGHDAPDCTFDTQGSHLETSGNLSPKSGPKDSSEGLGSTKLPVPLRRGVVQDWDS

MTAVWNRCFEDELRVDPDAAGTPVLITDAPLSSKQSREKSAEIMFEHFKVPGLYIANQTVLSLYACGKTRGLVVEVGEGA

TYSVPVFEGFALPHASTTLPLAGGDLTAYLRSMLKDRGHAFKNSIAHTAIIRGIKESLCAVSDNYEHDVLTDKSEYAYEL

PDGTALNIDARCRYSVGEALFKPSIVKGKTKLNSNNSPNEVGIADIALESISQCDSDLQPDLRSSIILSGGTSLLNGLKS

RMQSEMEKRCGGEPVEVVVDSQRKYASWIGGSMFASLGTFEQIYVTRQEYEDSGPSVMHRKCF

>gi|502589156|ref|WP_012826829.1| hypothetical protein [Haliangiumochraceum

MSDSYVFSSPIIIHPGSDTLQAGLADEEHPGSIFPNIVGRHKLAGLMEWVDQRVLCVGQEAIDQSATVLLRHPVWSGIVG

DWEAFAAVLRHTFYRALWVAPEEHPIVVTESPHVYRSFQLRREQLTRLLFETFHAPQVAVCSEAAMSLYACGLDTGLVVS

LGDFVSYVAPVHRGAIVDAGLTFLEPDGRSITEYLSRLLLERGHVFTSPEALRLVRDIKETLCYVADDVAKEAARNADSV

EATYLLPNGETLVLGNERFRCPEVLFHPDLLGWESPGLTDAVCNAIMKCDPSLQAELFGNIVVTGGGSLFPGLSERLQRE

LEQRAPAEAPVHLLTRDDRRHLPWKGAARFARDAQFAGFALTRQAYERHGAELIYQM

>gi|517209833|ref|WP_018398651.1| hypothetical protein [filamentous cyanobacterium ESFC-1]

MTTNSPIVIDSKPDYTRAGLSSGDTPTVIFQTDSVVSNPPMGFDGVVTNWDSMTAVLSHTFKQLGIDPSQHPILITEPPL

NPKLNREKMTQIFFETFNVTKLYMIMGEILALYDAGKTTGTTVYFSKNSRFVIVVPSYQGYAYSNGIIHSSFDENNLSDR

EIQKISDMIFRSINQCDLELRSDLFQNIILTGSIFPELAAKLKNEVVKLALPVYTANVSEITEPENGAWRGGDKFAPLLD

DYGTWMTKQEYTQYGATLVHRKCMS

>gi|639549179|ref|WP_024696905.1| actin [Pseudomonas syringae]

MDTKFVVMDISPATIQAGWRGEAVPSVEVPVPEKIFDEQGRIVGWEAFSTLLNTVLLDKLKLPSSDEVTVIYVVPSLTPK

ASKEKLLQLSFDNENYGLEGVFLKSAASAILFSLDRTKTTLEDATVPSVTGVAIRTDKKTAIITVDPVFQDTLLSQASRI

LTRNNWESSFDPQLALSDDSISQLADTILQAVLKTDVDIRSLLYKNLVLSGSRVNEPGFCEKLKKSITEKAPKGTLIGMT

LDPKPESAAWRGAAMYAASEGFAEETISMQEYNEAGPSIVHGKCPF

>gi|495377034|ref|WP_008101745.1| actin [Verrucomicrobiae bacterium DG1235]

MSITTSKTAPKKTKAKKTTNDLLLVGLDLGTNTTCIQVAKPTTGKVDASELIPSIVGYTTEGILPGIIPGDATTLYGELA

LKYKLHLNLVQPLRDGIIADMDAARDFLVHIKQTAGISPDAEVRAVIGMPANADAEARENTRLAVTGIFDKVILIPEPFL

AALGSREEAQLVKSDYVDPVCNSLFVDIGAGSTDVCLIQGYYPSAEDQISFAFAGDSVDEMIQESLLNKYPDSQVSIAKC

REIKEKHSFVQGSTKDATHPVMISGKIKQLEVADAVGAACDKLLGKIYEAVRALIVKADPDSIPDLLQNIIITGGGSMIK

GLDASLQKLLSDEGFEGCVVTSVGEDYKTRVASGAIKAAFQAKERQWQTLLR

>gi|504619238|ref|WP_014806340.1| rod shape-determining protein Mbl [Anaerobaculum mobile]

MFRFLSGLFAQDIGIDLGTANVVVYLKGKGIVVDEPSVVAYRKSSNARDEIIAIGRRAKEMIGKTPQSIVTLRPLKHGVI

ADFDVTEAMLQHYIRKINGDRRILARPRVVVSVPACVTEVERKAVIDACLGGGAREAYIIEEPLAAALGAGLPIHEPKGT

MIINIGGGTTEVAVLSLGGIVESSSLRVAGDDIDEAIIELLRQNFKMCIGEAMAEEIKISVGSVCALDEELSIEVKGRDL

SNGLPKTLTITSSDVRVALEPLIKRMEETVRDTLERTSPELVRDIVERGIILTGGGALLKGLPKRLSASLNIPVMCAEQP

LYVVAMGIGKVLDELEKMKRALISI

>gi|505369522|ref|WP_015556624.1| rod shape-determining protein MreB [Fretibacteriumfastidiosum]

MFKYLSGVLGLDVGIDLGSANIVVYVKDKGIVLSEPSTVAIRKLPRGGGLEVIAVGREAKAMSGKTPGGVEAIWPLQDGV

IANFDMTEELIRYCLRRAGGSGSFMVHPRVVISVPAEVTEVERKAVVDATLGAGASEAYVVEEPISAALGVGLPIDRPNG

CMIIDIGGGTSEVSVISLGGIVVTSSLRTAGRTMDNAIIAMIRQRYALLIGETTAEEVKNTIGSALPLSPELEMSVKGRD

LSDGLPKSDMVSSLEVREALEPIFLSIEDMVKVALEKTPPELAKDIVDRGIVLSGGVALMRGFAERLSRTINTPVIVAEE

PLFSVALGVGKILDNLGTMRRVLMSVERGSR

>gi|356601823|gb|AET24913.1| magnetosome protein [CandidatusDesulfamplusmagnetomortis BW-1]

MGTTKAINVGIDLGTSRTVIACDNGIRTFVPSFVGYAKDAVTEKMLGKKIVFGNEALKYKMGLELYKPIENGVIRYSDES

DEETDGYKKSLEVSRELLKHVVELAVKDEKADNLIIRGVVGAPALATLNNKKAILNITKGILNDVMVVSEPFTVAYHLGC

LYNYLIIDIGAGTVDLCRMHGTIPSPEDQITSTLAGDHIDRTFLKLVHEKYEEANLTVNMIKRFKEENAFVSTQGERVTI

DIPVHGKPRTYDVTDELRQACKEIVPDIVEGIRKLVATFDPEYQNELKGNIILAGGGSQIVGLKKEIETYMLETLGYGRV

TKIEEPLFAGANGALMLARDMPEEYWDEIADPE

>gi|501344311|ref|WP_012375946.1| actin [Opitutusterrae]

MIPASTEKPVPSAAIPANAPNVAVSKPAAPGTRTKTVLVGFDFGTNKSCVLAGTAGATDIAISKIVPTVV

GYVKEGIVDGIVAGNRSVLFGDDALQNRLHARLVAPMEHGVIAHPDAARDFVQHLRSLADPSGQAEIRAV

VGVPANATEQAREDVRRCAFGIFDRILLIPEPFLAALGYRDDARLGQSNYIDPVVNSLFIDIGGGTSDIC

LVQGYFPGPDDQISIPFAGDAIDQLLQEELNRTYPNNGLSLHKVREIKEAHGYVGPSRKPLDVKVVIGGK

AHTLELGDTLARACNALIDKIYPALTTLIQRASSDSVVTLLQNIIITGGGSQIKGIDTLLQKKLTEDGFE

SPKVRLAGHDYKRYVALGALKAARAARENQWQVLLG

>gi|545620937|ref|WP_021744492.1| putative Mbl protein [Leptotrichia sp. oral taxon 879]

MGAFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGKLIAVGREAREMLGKTPESIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVNISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNRFDEDIVRYVKRKYNLLIGDRTAEKIKKEMATAIKVQSPEVMEIKGRDLESG

IPNTVEINANEIYEAIEDSLFQIVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIKNFIDMMEKEVGIKVFLSPNPLDS

VVLGGGAAFDNKKLLRTLQMKEN

>gi|493858132|ref|WP_006804969.1| rod shape-determining protein MreB [Leptotrichiahofstadii]

MGAFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGKLIAVGREAREMLGKTPDSIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVDISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNRFDEDIVRYVKRKYNLLIGDRTAEKIKKEMATALKVQSPEVMEIKGRDLESG

IPNTVEINANEIYEAIEDSLFQIVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIKNFIDMMEKEVGIKVFLSPNPLDS

VVLGGGVAFDNKKLLRTLQMKEN

>gi|750004295|ref|WP_040348174.1| rod-share determining protein MreBH [Anaerobaculumhydrogeniformans]

MFRFLSGLFARDMGIDLGTANVVVYQKGKGIVVDEPSVVAYRKSLNSKDEIIAIGMRAKEMIGKTPQSIVTLRPLKHGVI

ANFDVTEAMLQYYIRKINGDRRLFAHPRVVVSVPACVTEVERKAVIDACLGGGAKEAYIIEEPLAAALGAGLPIHEPKGS

MVINIGGGTTEVAVLSLGGIVESSSLRVAGDDIDEAIIELLRQSFKMCIGEATAEEIKIALGSACPLDEEPSTEVRGRDL

SNGLPKSLTITSSDVRVALEPMIKRIEETVRETLERTSPELVRDIVERGIILTGGGAMLKGLPKRLSSSLNIPVMCADQP

LYAVAIGIGKVLSELERMKRILISV

>gi|506250485|ref|WP_015770260.1| rod shape-determining protein MreB [Leptotrichiabuccalis]

MGAFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGKLIAVGREAREMLGKTPDSIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVDISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNKFDEDIVKYVKRKYNLLIGDRTAEKIKKELASAIKYQTPDIMEIKGRDLESG

IPNTVEVNANEIYEAIEDSLFQIVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIKNFIDMMEQEVGIKVFLSPNPLDS

VVLGGGAAFDNKKLLRTLQMKEN

>gi|736247219|ref|WP_034326962.1| rod-share determining protein MreBH [Aminobacterium mobile]

MFGTDVGIDLGTANIVVYVKGKGIVINEPSAVAIRKKTKGGGNIEVIAVGGEAKAMAGKTPTGVYTIWPMQDGVIANFEM

TEELIRHCLRKATGGNMVLSHPRVVISVPAEVTEVERKAVIDATLGAGAREAYVVDEPVAAALGVGLPIKEPRGSMILDI

GGGTSEVAVLSLGGIVVTNSLRTAGRDMDEAIIAMLRQKYALLIGETTAEEVKIEIGSALPLESELEMAVKGRDLADGLP

KADAVTSSEVREALEPIVRRIEDMVKVALEQTPPELSKDIVDQGIILSGGISLLRGLSQRLSRSLSTPVIVAEDPLFAVA

RGVGKILENLDDMKKVLLSVEKGAQ

>gi|545661929|ref|WP_021768479.1| putative Mbl protein [Leptotrichia sp. oral taxon 225]

MGAFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGKLIAVGREAREMLGKTPDSIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVNISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNKFDEDIVKYVKRKYNLLIGDRTAEKIKKEMATAIKLQSPEVMEIKGRDLESG

IPNTVEINANEIYEAIEDSLFQIVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIQNFVDMMEKEVGIKVFLSPNPLDS

VVLGGGAAFDNKKLLRTLQMKEN

>gi|652353257|ref|WP_026749423.1| rod shape-determining protein MreB [Leptotrichiatrevisanii]

MGAFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGKLIAVGREAREMLGKTPDSIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVNISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNRFDEDIVRYVKRKYNLLIGDRTAEKIKKEMATALKVQSPEVMEIKGRDLESG

IPNTVEINANEIYEAIEDSLYQVVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIKNFVDMMEKEVGIKVFLSPNPLDS

VVLGGGAAFDNKKLLRTLQMKEN

>gi|657199704|ref|WP_029315216.1| rod shape-determining protein MreB [Acaryochloris sp. CCMEE 5410]

MGVDLGTANTLVYVKGKGILVRNPSVIAVDAENQSVIAVGEKAQRLGGRQPSNILVRYPLRDGVITDLDIAYKLLQQVLE

QAQISRSLFPPRLIMAVPNGATTIEQRALQDIAEQAGAKEVFIIDEAIAAAIGAGLPIDKPLGNMIVDIGGGTTEVAVLS

LCGVLSSQSLRVAGAQMDDVIYQSLKRDYDFWVGEQTAQSIKHQLGSAEFNSQWDNVKMEIGGCSLNSRLPKQLTLTGME

VREMLHKEVALIARTVQQTLEQTTPEIASDIYTNGLMLCGGGSLLKGLDEFICRETGVFVHVAPNPMDCVALGIGEILEN

RQRWERVWKHWKRQ

>gi|517312299|ref|WP_018498977.1| rod shape-determining protein MreB [Leptotrichiawadei]

MGVFDFVKIFRVNKSISIDLGTANILIYDKQRKKIVLNEPSVVARDRKTGRLIAVGKEAREMLGKTPESIQAIKPLQDGV

IADIDSTKEMITYFIHKIYGNSLFKPEVMICVPIEVTSVERKALFDAVKGAKKTYIIEEGRAAIIGSGVDISQPEGSMVI

DIGGGSTDIAILSLDEIIASKSIRIAGNRFDEDIVRYVKRKYNLLIGDRTAEKIKKELGSALKVQSPEVMEIKGRDLETG

IPNTVEVNANEIYEAIEDSLFQIVNSTKEVLEKCPPELAADILDNGIVMTGGGSLIRNFIDMMEKEVGIKVFLSPNPLDS

VVLGGGAAFDNKKLLRTLQMKEN