Supp Table 1. Amplication and variability of 21 microsatellite loci for Ramphocelusbresilius. Allelic richness (AR14) and the number of private alleles (P14) were determined by rarefaction based on 14 individuals (the smallest genotyped sample at a locus) for the 6 populations genotyped. Delta refers to the distance between R. bresilius and the source species, based on the DNA-DNA melting temperature (Δ Tm) hybridization distance from Sibley and Ahlquist (1990); n refers to the number of unrelated individuals genotyped; K, number of alleles; nsp, indicates non-specific products.

Locus / Source species / Family / Delta / Exp. size (bp) / Obs. size (bp) / K
Source
species / n
Source
species / K / n / AR14 / P14
Escμ1 / Emberizaschoeniclus / Fringillidae / 6 / 148 / nsp / 9 / 10 / nsp / 8
Escμ2 / Emberizaschoeniclus / Fringillidae / 6 / 210 / 149-157 / 7 / 8 / 2 / 177 / 1.95
Escμ3 / Emberizaschoeniclus / Fringillidae / 6 / 158 / 158 / 7 / 10 / 1 / 8
Escμ4 / Emberizaschoeniclus / Fringillidae / 6 / 152 / 150-174 / 12 / 10 / 11 / 177 / 6.37 / 0.31
Escμ6 / Emberizaschoeniclus / Fringillidae / 6 / 196 / 140-145 / 10 / 10 / 5 / 177 / 2.95 / 0.3
LOX1 / Loxiascotica / Fringillidae / 6 / 341 / 336-400 / 31 / 50 / 35 / 177 / 11.23 / 2.20
Mcyµ4 / Maluruscyaneus / Maluridae / 12.8 / 170 / 172-179 / 18 / 17 / 5 / 177 / 5.13 / 0.15
Mme8 / Melospizamelodia / Fringillidae / 6 / 208 / nsp / 13 / 670 / nsp / 8
Pca3 / Paruscaeruleus / Paridae / 11.1 / 191 / 142-161 / 7 / 8 / 3 / 177 / 1.6
Pca4 / Paruscaeruleus / Paridae / 11.1 / 191 / nsp / 7 / 8 / nsp / 8
Pca8 / Paruscaeruleus / Paridae / 11.1 / 295 / nsp / 14 / 8 / nsp / 8
Pca9 / Paruscaeruleus / Paridae / 11.1 / 131 / 151 / 8 / 8 / 1 / 8
Pdoµ1 / Passer domesticus / Passeridae / 10 / 154 / 159 / 10 / 40 / 1 / 11
Pdoµ3 / Passer domesticus / Passeridae / 10 / 140 / nsp / 10 / 48 / nsp / 8
Pdoµ4 / Passer domesticus / Passeridae / 10 / 375 / nsp / 16 / 48 / nsp / 8
Pdoµ5 / Passer domesticus / Passeridae / 10 / 230 / 206 / 10 / 26 / 1 / 11
Pdoµ6 / Passer domesticus / Passeridae / 10 / 330 / 200 / 15 / 48 / 1 / 11
Pdo9 / Passer domesticus / Passeridae / 10 / 122 / nsp / 14 / 45 / nsp / 11
Pdo10 / Passer domesticus / Passeridae / 10 / 125 / 127-137 / 10 / 45 / 4 / 177 / 2.41
POCC6 / Phylloscopus / Sylviidae / 11.1 / 174 / 188-192 / 8 / 16 / 4 / 177 / 2.9 / 0.08
Ppi2 / Pica pica / Corvidae / 12.8 / 244 / 223 / 16 / 173 / 1 / 8