April 12, 2005
SUBJECT: BARC Poster Day 2005
TO: Institute/Center Directors, Research Leaders, Scientists, New-hires,
Research Associates and Visiting Scientists in the Beltsville Area
FROM:Rick Jones and Qi Huang
Co-chairs, BARC Poster Day 2005
The Sixteenth Annual BARC Poster Day is scheduled for Thursday, May 12, 2005. BARC Poster Day is an opportunity for Research Associates, Visiting Scientists, and recently hired Scientists to present their research findings in a multi-disciplinary setting. The event also provides an excellent opportunity to meet and interact with other members
in the research community at BARC.
The Area Office will give awards to the poster presentations judged to be the best by a scientific peer panel. Specific judging criteria will be announced in a few weeks.
Posters must be set up by COB Tuesday, May 10 so that judging can take place on the following day. Posters should be constructed to fit on a 46"x 70" (H x W) surface. Poster surfaces are velcro-compatible; presenters will need to provide velcro.
Preparation and Submission of Abstracts (a sample Abstract is shown below):
1.Abstract should be produced with WordPerfect or Word using Times New Roman 12 point font.
1.Top and side margins should each be 1". Please justify the right margin.
1.Title should begin on line 1 in boldface caps. Italicize species names.
1.Leave one blank line; then type the authors' names. First name, middle initial and last name with presenting author's name denoted by an asterisk, i.e. Marissa L. Brown*, John P. Pembrooke, and Heather L. Stone.
1.On the next line, list laboratory and institution affiliations for authors i.e. USDA-ARS Fruit Laboratory, PSI, Beltsville, MD 20705; Department of Horticulture, Michigan State University, East Lansing, MI 48824.
1.Leave one blank line.
1.Abstract text should begin on a new line with no indent. The text can be no more than 30 single-spaced lines.
1.No figures, columns, or tables, please.
1.On a separate page, provide the name, address, phone, fax and e-mail address of the contact person for all matters pertaining to the BARC Poster Day presentation.
1.A hard copy of the abstract, a signed ARS-115 form, and an electronic version of the abstract (diskette or e-mail attachment) in the specified format should be submitted by COB Friday, April 22 to Rick Jones, Building 10A, Room 311, BARC-W () (504-8395).
1.Abstracts submitted without a signed ARS-115 form will not be accepted.
Sample abstract:
Serial analysis of gene expression (SAGE) during elongation of periimplantation porcineembryos
Le Ann Blomberg*, Ezhou L. Long, Tad S. Sonstegard, Curt P. Van Tassel, John R. Dobrinsky, Kurt A. Zuelke.
USDA-ARS, Biotechnology and Germplasm and Bovine Functional Genomics Laboratories, Beltsville, MD
In the pig, ~ 30% of embryos are lost prior to gestational day 20; the major loss occurs between day 11 (D11) and day 12 (D12) when embryos undergo rapid elongation prior to implantation. Elucidating the array of genes expressed in D11 and D12 embryos would provide insight regarding essential temporally regulated genes, which could serve as potential markers of development efficacy. To determine qualitative and quantitative gene expression of D11 and D12 embryos, SAGE was done. Total RNA from in vivo derived D11 and D12 embryos was used to construct individual SAGE tag libraries. Analysis of the libraries yielded a total of 42,389 tags (D11) and 42,392 tags (D12) representing 14,464 and 13,098 putative genes, respectively. Comparative statistical analysis of the SAGE tag frequencies indicated that at p<0.05 significance and p<0.001 significance, 434 tags and 87 tags, respectively, were differentially expressed between D11 and D12. The SAGE tags, annotated following blasts on NCBI and TIGR species indices databases, were clustered into functional groups. Real-time PCR confirmed differential expression of several transcripts between D11 and D12. Despite the paucity of porcine sequence data, SAGE proved effective in identifying genes potentially crucial to embryo development and should aid in providing a more comprehensive picture of the porcine transcriptome. Supported by USDA ARS CRIS Project No. 1265-31000-082.