SUPPLEMENTAL MATERIAL

Online Resource 1

Primers used for RT-PCR to study gene expression during the CSVT

GB: GenBank accession number, GI: GenInfo identifier, Tm: melting temperature of the given primer, PS: Predicted Size of the amplicon, ADH: alcohol dehydrogenase, ALDH: aldehyde dehydrogenase, 4ADHs: universal primer designed to amplify all the four ADHs, LDH: lactate dehydrogenase, NR: nitrate reductase.

Online Resource 2

Statistical analysis of Fv/Fm values of the freezing experiment carried out with T2 plants at -12°C.

All transgenic lines (L1-L9) were evaluated in comparison with the springbarley cultivar Golden Promise (GP).

Descriptives
Cold hardened
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 15 / .73393 / .023365 / .006033 / .72099 / .74687 / .693 / .772
L2 / 15 / .73587 / .019526 / .005042 / .72505 / .74668 / .703 / .764
L3 / 15 / .74687 / .017740 / .004580 / .73704 / .75669 / .707 / .774
L4 / 15 / .73540 / .024695 / .006376 / .72172 / .74908 / .687 / .766
L5 / 15 / .74287 / .023311 / .006019 / .72996 / .75578 / .701 / .773
L7 / 15 / .73813 / .017659 / .004560 / .72835 / .74791 / .707 / .767
L8 / 15 / .72407 / .024300 / .006274 / .71061 / .73752 / .670 / .756
L9 / 15 / .73253 / .020573 / .005312 / .72114 / .74393 / .699 / .780
GP / 15 / .74573 / .016312 / .004212 / .73670 / .75477 / .719 / .770
Nure / 15 / .74467 / .030575 / .007894 / .72773 / .76160 / .653 / .784
Total / 150 / .73801 / .022552 / .001841 / .73437 / .74165 / .653 / .784
Post Hoc Tests; Multiple Comparisons
Cold hardened
(I) vonal / (J) vonal / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

LSD

GP / L1 / .011800 / .008101 / .147 / -.00422 / .02782
L2 / .009867 / .008101 / .225 / -.00615 / .02588
L3 / -.001133 / .008101 / .889 / -.01715 / .01488
L4 / .010333 / .008101 / .204 / -.00568 / .02635
L5 / .002867 / .008101 / .724 / -.01315 / .01888
L7 / .007600 / .008101 / .350 / -.00842 / .02362
L8 / .021667* / .008101 / .008 / .00565 / .03768
L9 / .013200 / .008101 / .105 / -.00282 / .02922
Nure / .001067 / .008101 / .895 / -.01495 / .01708

* The mean difference is significant at the 0.05 level.

Descriptives
after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 15 / .72133 / .025637 / .006619 / .70714 / .73553 / .675 / .774
L2 / 15 / .73300 / .013158 / .003397 / .72571 / .74029 / .700 / .751
L3 / 15 / .73933 / .023157 / .005979 / .72651 / .75216 / .684 / .781
L4 / 15 / .73580 / .019277 / .004977 / .72512 / .74648 / .690 / .761
L5 / 15 / .74793 / .021956 / .005669 / .73577 / .76009 / .704 / .792
L7 / 15 / .73313 / .031398 / .008107 / .71575 / .75052 / .657 / .768
L8 / 15 / .72980 / .014756 / .003810 / .72163 / .73797 / .710 / .755
L9 / 15 / .73347 / .012900 / .003331 / .72632 / .74061 / .717 / .761
GP / 15 / .73093 / .032477 / .008386 / .71295 / .74892 / .629 / .764
Nure / 15 / .75667 / .017855 / .004610 / .74678 / .76655 / .707 / .774
Total / 150 / .73614 / .023565 / .001924 / .73234 / .73994 / .629 / .792
Post Hoc Test;Multiple Comparisons
after the stress
(I) vonal / (J) vonal / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

LSD

GP / L1 / .009600 / .008135 / .240 / -.00648 / .02568
L2 / -.002067 / .008135 / .800 / -.01815 / .01402
L3 / -.008400 / .008135 / .304 / -.02448 / .00768
L4 / -.004867 / .008135 / .551 / -.02095 / .01122
L5 / -.017000* / .008135 / .038 / -.03308 / -.00092
L7 / -.002200 / .008135 / .787 / -.01828 / .01388
L8 / .001133 / .008135 / .889 / -.01495 / .01722
L9 / -.002533 / .008135 / .756 / -.01862 / .01355
Nure / -.025733* / .008135 / .002 / -.04182 / -.00965

* The mean difference is significant at the 0.05 level.

Descriptives
24h after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 15 / .57033 / .211149 / .054518 / .45340 / .68726 / .129 / .791
L2 / 15 / .47833 / .250329 / .064635 / .33971 / .61696 / .033 / .765
L3 / 15 / .51133 / .173997 / .044926 / .41498 / .60769 / .086 / .730
L4 / 15 / .63460 / .092535 / .023892 / .58336 / .68584 / .474 / .743
L5 / 15 / .70073 / .082093 / .021196 / .65527 / .74619 / .429 / .777
L7 / 15 / .53773 / .181930 / .046974 / .43698 / .63848 / .214 / .732
L8 / 15 / .69387 / .103383 / .026693 / .63662 / .75112 / .374 / .756
L9 / 15 / .62967 / .190676 / .049232 / .52407 / .73526 / .030 / .771
GP / 15 / .41407 / .191088 / .049339 / .30825 / .51989 / .048 / .645
Nure / 15 / .75073 / .043591 / .011255 / .72659 / .77487 / .625 / .799
Total / 150 / .59214 / .189955 / .015510 / .56149 / .62279 / .030 / .799
Post Hoc Tests; Multiple Comparisons
24h after the stress
(I) vonal / (J) vonal / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

LSD

GP / L1 / -.156267* / .060154 / .010 / -.27519 / -.03734
L2 / -.064267 / .060154 / .287 / -.18319 / .05466
L3 / -.097267 / .060154 / .108 / -.21619 / .02166
L4 / -.220533* / .060154 / .000 / -.33946 / -.10161
L5 / -.286667* / .060154 / .000 / -.40559 / -.16774
L7 / -.123667* / .060154 / .042 / -.24259 / -.00474
L8 / -.279800* / .060154 / .000 / -.39873 / -.16087
L9 / -.215600* / .060154 / .000 / -.33453 / -.09667
Nure / -.336667* / .060154 / .000 / -.45559 / -.21774

* The mean difference is significant at the 0.05 level.

Online Resource 3

Statistical analysis of Fv/Fm values of the second freezing experiment at -11°C and -12°C.

Selected T3 transgenic lines were evaluated in comparison with the spring barley cultivar Golden Promise and with the winter barley cultivar Nure.

*The mean difference is significant at the 0.05 level.

Descriptives
-11°C; cold hardened
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
lines / Lower Bound / Upper Bound
L1 / 20 / .77580 / .012361 / .002764 / .77001 / .78159 / .750 / .798
L5 / 20 / .77420 / .011478 / .002567 / .76883 / .77957 / .751 / .793
L8 / 20 / .77835 / .012014 / .002686 / .77273 / .78397 / .759 / .801
L9 / 20 / .78360 / .011066 / .002475 / .77842 / .78878 / .762 / .797
GP / 20 / .77490 / .016364 / .003659 / .76724 / .78256 / .730 / .798
Nure / 19 / .78853 / .010911 / .002503 / .78327 / .79379 / .768 / .806
Total / 119 / .77915 / .013296 / .001219 / .77674 / .78156 / .730 / .806
Post Hoc Tests; Multiple Comparisons
LSD / -11°C; cold hardened
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -.000900 / .003958 / .821 / -.00874 / .00694
L5 / .000700 / .003958 / .860 / -.00714 / .00854
L8 / -.003450 / .003958 / .385 / -.01129 / .00439
L9 / -.008700* / .003958 / .030 / -.01654 / -.00086
Nure / -.013626* / .004010 / .001 / -.02157 / -.00568
Descriptives
-11°C; after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 20 / .73755 / .038063 / .008511 / .71974 / .75536 / .643 / .789
L5 / 20 / .76210 / .014987 / .003351 / .75509 / .76911 / .727 / .785
L8 / 20 / .76170 / .013608 / .003043 / .75533 / .76807 / .731 / .780
L9 / 20 / .75310 / .020617 / .004610 / .74345 / .76275 / .703 / .778
GP / 20 / .73150 / .022388 / .005006 / .72102 / .74198 / .674 / .762
Nure / 19 / .77237 / .011051 / .002535 / .76704 / .77770 / .754 / .789
Total / 119 / .75289 / .025944 / .002378 / .74818 / .75760 / .643 / .789
Post Hoc Tests; Multiple Comparisons
LSD / -11°C; after the stress
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -.006050 / .006984 / .388 / -.01989 / .00779
L5 / -.030600* / .006984 / .000 / -.04444 / -.01676
L8 / -.030200* / .006984 / .000 / -.04404 / -.01636
L9 / -.021600* / .006984 / .002 / -.03544 / -.00776
Nure / -.040868* / .007075 / .000 / -.05488 / -.02685
Descriptives
-11°C; 24 h after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 20 / .54580 / .128124 / .028649 / .48584 / .60576 / .256 / .717
L5 / 20 / .61705 / .079680 / .017817 / .57976 / .65434 / .449 / .729
L8 / 20 / .61300 / .108082 / .024168 / .56242 / .66358 / .337 / .706
L9 / 20 / .59000 / .115913 / .025919 / .53575 / .64425 / .293 / .691
GP / 20 / .31655 / .131187 / .029334 / .25515 / .37795 / .137 / .582
Nure / 19 / .72447 / .034337 / .007877 / .70792 / .74102 / .628 / .765
Total / 119 / .56650 / .161969 / .014848 / .53709 / .59590 / .137 / .765
Post Hoc Tests; Multiple Comparisons
LSD / -11°C; 24 h after the stress
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -.229250* / .033365 / .000 / -.29535 / -.16315
L5 / -.300500* / .033365 / .000 / -.36660 / -.23440
L8 / -.296450* / .033365 / .000 / -.36255 / -.23035
L9 / -.273450* / .033365 / .000 / -.33955 / -.20735
Nure / -.407924* / .033802 / .000 / -.47489 / -.34096
Descriptives
-12°C; cold hardened
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 20 / .78825 / .011621 / .002598 / .78281 / .79369 / .764 / .804
L5 / 20 / .78775 / .007525 / .001683 / .78423 / .79127 / .771 / .801
L8 / 20 / .78500 / .007455 / .001667 / .78151 / .78849 / .760 / .793
L9 / 20 / .79180 / .011750 / .002627 / .78630 / .79730 / .767 / .817
GP / 20 / .78835 / .012167 / .002721 / .78266 / .79404 / .762 / .805
Nure / 20 / .79540 / .011673 / .002610 / .78994 / .80086 / .764 / .825
Total / 120 / .78942 / .010865 / .000992 / .78746 / .79139 / .760 / .825
Post Hoc Tests; Multiple Comparisons
LSD / -12°C; cold hardened
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / .000100 / .003341 / .976 / -.00652 / .00672
L5 / .000600 / .003341 / .858 / -.00602 / .00722
L8 / .003350 / .003341 / .318 / -.00327 / .00997
L9 / -.003450 / .003341 / .304 / -.01007 / .00317
Nure / -.007050* / .003341 / .037 / -.01367 / -.00043
Descriptives
-12°C; after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 20 / .72265 / .021617 / .004834 / .71253 / .73277 / .672 / .769
L5 / 20 / .75155 / .014136 / .003161 / .74493 / .75817 / .725 / .774
L8 / 20 / .73645 / .023365 / .005225 / .72551 / .74739 / .699 / .772
L9 / 20 / .73210 / .017508 / .003915 / .72391 / .74029 / .683 / .759
GP / 20 / .71670 / .027629 / .006178 / .70377 / .72963 / .659 / .762
Nure / 20 / .73970 / .041066 / .009183 / .72048 / .75892 / .659 / .792
Total / 120 / .73319 / .027639 / .002523 / .72820 / .73819 / .659 / .792
Post Hoc Tests; Multiple Comparisons
LSD / -12°C; after the stress
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -.005950 / .008134 / .466 / -.02206 / .01016
L5 / -.034850* / .008134 / .000 / -.05096 / -.01874
L8 / -.019750* / .008134 / .017 / -.03586 / -.00364
L9 / -.015400 / .008134 / .061 / -.03151 / .00071
Nure / -.023000* / .008134 / .006 / -.03911 / -.00689
Descriptives
-12°C; 24 h after the stress
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 20 / .36480 / .210166 / .046995 / .26644 / .46316 / .058 / .711
L5 / 20 / .47360 / .185328 / .041441 / .38686 / .56034 / .126 / .716
L8 / 20 / .40325 / .191645 / .042853 / .31356 / .49294 / .026 / .670
L9 / 20 / .41740 / .197172 / .044089 / .32512 / .50968 / .082 / .666
GP / 20 / .29355 / .216667 / .048448 / .19215 / .39495 / .046 / .681
Nure / 20 / .68570 / .049873 / .011152 / .66236 / .70904 / .589 / .768
Total / 120 / .43972 / .218411 / .019938 / .40024 / .47920 / .026 / .768
Post Hoc Tests; Multiple Comparisons
LSD / -12°C; 24 h after the stress
(I) line / (J) line / Mean Difference (I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -.071250 / .058246 / .224 / -.18663 / .04413
L5 / -.180050* / .058246 / .003 / -.29543 / -.06467
L8 / -.109700 / .058246 / .062 / -.22508 / .00568
L9 / -.123850* / .058246 / .036 / -.23923 / -.00847
Nure / -.392150* / .058246 / .000 / -.50753 / -.27677
Descriptives
lines / 1: -11°C
2: -12°C / Cold hardened / After the stress / 24 h after the stress
Mean / Std. Deviation / Mean / Std. Deviation / Mean / Std. Deviation
L1 / 1 / .77580 / .012361 / .73755 / .038063 / .54580 / .128124
2 / .78825 / .011621 / .72265 / .021617 / .36480 / .210166
Total / .78202 / .013415 / .73010 / .031471 / .45530 / .194721
L5 / 1 / .77420 / .011478 / .76210 / .014987 / .61705 / .079680
2 / .78775 / .007525 / .75155 / .014136 / .47360 / .185328
Total / .78098 / .011783 / .75683 / .015340 / .54532 / .158437
L8 / 1 / .77835 / .012014 / .76170 / .013608 / .61300 / .108082
2 / .78500 / .007455 / .73645 / .023365 / .40325 / .191645
Total / .78168 / .010428 / .74907 / .022796 / .50812 / .186722
L9 / 1 / .78360 / .011066 / .75310 / .020617 / .59000 / .115913
2 / .79180 / .011750 / .73210 / .017508 / .41740 / .197172
Total / .78770 / .012007 / .74260 / .021667 / .50370 / .182001
GP / 1 / .77490 / .016364 / .73150 / .022388 / .31655 / .131187
2 / .78835 / .012167 / .71670 / .027629 / .29355 / .216667
Total / .78162 / .015778 / .72410 / .025928 / .30505 / .177174
Nure / 1 / .78853 / .010911 / .77237 / .011051 / .72447 / .034337
2 / .79540 / .011673 / .73970 / .041066 / .68570 / .049873
Total / .79205 / .011689 / .75562 / .034274 / .70459 / .046772
Total / 1 / .77915 / .013296 / .75289 / .025944 / .56650 / .161969
2 / .78943 / .010865 / .73319 / .027639 / .43972 / .218411
Total / .78431 / .013160 / .74300 / .028515 / .50284 / .202221
Multiple Comparisons
LSD
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

Cold hardened

GP / L1 / -.00040 / .002589 / .877 / -.00550 / .00470
L5 / .00065 / .002589 / .802 / -.00445 / .00575
L8 / -.00005 / .002589 / .985 / -.00515 / .00505
L9 / -.00607* / .002589 / .020 / -.01118 / -.00097
Nure / -.01043* / .002605 / .000 / -.01556 / -.00529
The error term is Mean Square(Error) = 0.000.

After the stress

GP / L1 / -.00600 / .006447 / .999 / -.02549 / .01349
L5 / -.03272* / .004763 / .000 / -.04722 / -.01823
L8 / -.02497* / .005459 / .000 / -.04147 / -.00848
L9 / -.01850* / .005343 / .013 / -.03465 / -.00235
Nure / -.03152* / .006850 / .000 / -.05227 / -.01076
The error term is Mean Square(Error) = 0.001.

24 h after the stress

GP / L1 / -.15025* / .041625 / .008 / -.27600 / -.02450
L5 / -.24028* / .037581 / .000 / -.35382 / -.12673
L8 / -.20307* / .040699 / .000 / -.32600 / -.08015
L9 / -.19865* / .040161 / .000 / -.31995 / -.07735
Nure / -.39954* / .028998 / .000 / -.48923 / -.30984
The error term is Mean Square(Error) = 0.023.
Tests of Between-Subjects Effects
Cold hardened
Source / Type III Sum of Squares / df / Mean Square / F / Sig.
Intercept / Hypothesis / 146.996 / 1 / 146.996 / 23671.608 / .004
Error / .006 / 1 / .006
lines / Hypothesis / .004 / 5 / .001 / 7.306 / .024
Error / .001 / 5 / .000
temperature / Hypothesis / .006 / 1 / .006 / 57.253 / .001
Error / .001 / 5.001 / .000
Lines*temperature / Hypothesis / .001 / 5 / .000 / .809 / .544
Error / .030 / 227 / .000
After the stress
Source / Type III Sum of Squares / df / Mean Square / F / Sig.
Intercept / Hypothesis / 131.957 / 1 / 131.957 / 5599.649 / .009
Error / .024 / 1 / .024
lines / Hypothesis / .037 / 5 / .007 / 11.219 / .010
Error / .003 / 5 / .001
temperature / Hypothesis / .024 / 1 / .024 / 36.026 / .002
Error / .003 / 5.001 / .001
Lines*temperature / Hypothesis / .003 / 5 / .001 / 1.138 / .341
Error / .131 / 227 / .001
24 h after the stress
Source / Type III Sum of Squares / df / Mean Square / F / Sig.
Intercept / Hypothesis / 60.641 / 1 / 60.641 / 61.865 / .081
Error / .980 / 1 / .980
lines / Hypothesis / 3.323 / 5 / .665 / 10.894 / .010
Error / .305 / 5 / .061
temperature / Hypothesis / .980 / 1 / .980 / 16.069 / .010
Error / .305 / 5.000 / .061
Lines*temperature / Hypothesis / .305 / 5 / .061 / 2.702 / .022
Error / 5.125 / 227 / .023

Online Resource 4

Statistical analysis of CSVT data

Analysis of data on shoot length, percentage of seedlings with high or medium vigour, abnormal seeds, and none germinated seeds.

Descriptives
Shoot length
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 8 / 32.9973 / 8.605555 / 3.042523 / 25.80292 / 40.19177 / 21.120 / 46.480
L5 / 8 / 39.8115 / 5.051418 / 1.785946 / 35.58845 / 44.03464 / 31.476 / 49.435
L8 / 8 / 34.1345 / 3.678253 / 1.300459 / 31.05943 / 37.20962 / 28.905 / 40.667
GP / 8 / 19.2774 / 3.733044 / 1.319830 / 16.15653 / 22.39833 / 15.062 / 26.682
Total / 32 / 31.5552 / 9.350541 / 1.652958 / 28.18398 / 34.92644 / 15.062 / 49.435
LSD; Multiple Comparisons
Shoot length
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -13.719918* / 2.817774 / .000 / -19.49187 / -7.94797
L5 / -20.534114* / 2.817774 / .000 / -26.30606 / -14.76217
L8 / -14.857094* / 2.817774 / .000 / -20.62904 / -9.08515

* The mean difference is significant at the 0.05 level.

Descriptives
High vigour
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 8 / 91.4375 / 5.47356 / 1.93519 / 86.8615 / 96.0135 / 84.00 / 100.00
L5 / 8 / 89.4792 / 5.60430 / 1.98142 / 84.7939 / 94.1645 / 80.00 / 96.00
L8 / 8 / 77.7955 / 8.29471 / 2.93262 / 70.8609 / 84.7300 / 64.00 / 88.00
GP / 8 / 76.0000 / 10.02853 / 3.54562 / 67.6159 / 84.3841 / 64.00 / 88.00
Total / 32 / 83.6780 / 10.02344 / 1.77191 / 80.0642 / 87.2919 / 64.00 / 100.00
LSD; Multiple Comparisons
High vigour
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / -15.43750* / 3.79755 / .000 / -23.2164 / -7.6586
L5 / -13.47917* / 3.79755 / .001 / -21.2581 / -5.7002
L8 / -1.79545 / 3.79755 / .640 / -9.5744 / 5.9835

* The mean difference is significant at the 0.05 level.

Descriptives
Medium vigour
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 8 / 3.00 / 2.828 / 1.000 / .64 / 5.36 / 0 / 8
L5 / 8 / 3.00 / 2.828 / 1.000 / .64 / 5.36 / 0 / 8
L8 / 8 / 7.57 / 4.447 / 1.572 / 3.85 / 11.29 / 4 / 16
GP / 8 / 5.00 / 4.140 / 1.464 / 1.54 / 8.46 / 0 / 12
Total / 32 / 4.64 / 3.948 / .698 / 3.22 / 6.07 / 0 / 16
LSD; Multiple Comparisons
Medium vigour
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / 2.000 / 1.819 / .281 / -1.73 / 5.73
L5 / 2.000 / 1.819 / .281 / -1.73 / 5.73
L8 / -2.568 / 1.819 / .169 / -6.29 / 1.16

* The mean difference is significant at the 0.05 level.

Descriptives
Abnormal seeds
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 8 / 1.12 / 1.126 / .398 / .18 / 2.07 / 0 / 3
L5 / 8 / .88 / 1.126 / .398 / -.07 / 1.82 / 0 / 3
L8 / 8 / .50 / .756 / .267 / -.13 / 1.13 / 0 / 2
GP / 8 / 2.25 / 1.389 / .491 / 1.09 / 3.41 / 0 / 4
Total / 32 / 1.19 / 1.256 / .222 / .73 / 1.64 / 0 / 4
LSD; Multiple Comparisons
Abnormal seeds
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / 1.125 / .561 / .055 / -.02 / 2.27
L5 / 1.375* / .561 / .021 / .23 / 2.52
L8 / 1.750* / .561 / .004 / .60 / 2.90

* The mean difference is significant at the 0.05 level.

Descriptives
Non germinated seeds
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum / Maximum
Lower Bound / Upper Bound
L1 / 8 / .25 / .463 / .164 / -.14 / .64 / 0 / 1
L5 / 8 / 1.00 / .756 / .267 / .37 / 1.63 / 0 / 2
L8 / 8 / 3.12 / 1.959 / .693 / 1.49 / 4.76 / 0 / 6
GP / 8 / 2.50 / 1.604 / .567 / 1.16 / 3.84 / 0 / 5
Total / 32 / 1.72 / 1.727 / .305 / 1.10 / 2.34 / 0 / 6
LSD; Multiple Comparisons
Non germinated seeds
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound
GP / L1 / 2.250* / .590 / .029 / .22 / 4.28
L5 / 1.500 / .627 / .207 / -.55 / 3.55
L8 / -.625 / .895 / .984 / -3.38 / 2.13

Online Resource 5

Statistical analysis of the enzyme activity measurements in the CSVT.

Alpha-amylase (AMY), lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT) activity in three transgenic (L1, L5, L8) and in the wild type Golden Promise (GP) plants under hypoxia (H), hypoxia and cold (H+C) or in the shoot tissue.

AMY; Descriptives
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean
Lower Bound / Upper Bound
H / L1 / 3 / 113.67 / 19.604 / 11.319 / 64.97 / 162.37
L5 / 3 / 97.67 / 12.583 / 7.265 / 66.41 / 128.92
L8 / 3 / 107.00 / 18.358 / 10.599 / 61.40 / 152.60
GP / 3 / 48.67 / 11.930 / 6.888 / 19.03 / 78.30
Total / 12 / 91.75 / 29.934 / 8.641 / 72.73 / 110.77
H+C / L1 / 3 / 89.67 / 22.591 / 13.043 / 33.55 / 145.78
L5 / 3 / 98.33 / 17.010 / 9.821 / 56.08 / 140.59
L8 / 3 / 62.67 / 15.275 / 8.819 / 24.72 / 100.61
GP / 3 / 76.67 / 11.590 / 6.692 / 47.87 / 105.46
Total / 12 / 81.83 / 20.266 / 5.850 / 68.96 / 94.71
Shoot / L1 / 3 / 219.00 / 38.354 / 22.143 / 123.72 / 314.28
L5 / 3 / 92.67 / 17.474 / 10.088 / 49.26 / 136.07
L8 / 3 / 193.67 / 22.053 / 12.732 / 138.88 / 248.45
GP / 3 / 105.33 / 21.939 / 12.667 / 50.83 / 159.83
Total / 12 / 152.67 / 61.239 / 17.678 / 113.76 / 191.58
LSD; Multiple Comparisons of AMY results
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

„H”

GP / L1 / -65.000* / 13.051 / .001 / -95.10 / -34.90
L5 / -49.000* / 13.051 / .006 / -79.10 / -18.90
L8 / -58.333* / 13.051 / .002 / -88.43 / -28.24

„H+C”

GP / L1 / -13.000 / 13.948 / .379 / -45.16 / 19.16
L5 / -21.667 / 13.948 / .159 / -53.83 / 10.50
L8 / 14.000 / 13.948 / .345 / -18.16 / 46.16

Shoot

GP / L1 / -113.667* / 21.385 / .001 / -162.98 / -64.35
L5 / 12.667 / 21.385 / .570 / -36.65 / 61.98
L8 / -88.333* / 21.385 / .003 / -137.65 / -39.02

* The mean difference is significant at the 0.05 level.

LDH.Descriptives
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum
Lower Bound / Upper Bound
H / L1 / 3 / 102.3333 / 14.52714 / 8.38725 / 66.2459 / 138.4207 / 90.90
L5 / 3 / 119.7000 / 10.33004 / 5.96405 / 94.0388 / 145.3612 / 108.07
L8 / 3 / 74.2367 / 10.26122 / 5.92432 / 48.7464 / 99.7269 / 65.14
GP / 3 / 98.8833 / 3.66312 / 2.11490 / 89.7836 / 107.9830 / 94.69
Total / 12 / 98.7883 / 19.14275 / 5.52603 / 86.6256 / 110.9511 / 65.14
H+C / L1 / 3 / 120.3967 / 6.74564 / 3.89460 / 103.6396 / 137.1538 / 112.82
L5 / 3 / 125.3333 / 4.10104 / 2.36774 / 115.1458 / 135.5209 / 122.72
L8 / 3 / 77.7967 / 10.70404 / 6.17998 / 51.2064 / 104.3870 / 65.65
GP / 3 / 103.6133 / 6.88248 / 3.97360 / 86.5163 / 120.7104 / 96.84
Total / 12 / 106.7850 / 20.42200 / 5.89532 / 93.8095 / 119.7605 / 65.65
Shoot / L1 / 3 / 263.6400 / 7.09002 / 4.09343 / 246.0274 / 281.2526 / 256.56
L5 / 3 / 235.6733 / 6.03517 / 3.48440 / 220.6811 / 250.6655 / 228.76
L8 / 3 / 220.5567 / 3.31515 / 1.91400 / 212.3214 / 228.7920 / 216.74
GP / 3 / 223.4200 / 7.35008 / 4.24357 / 205.1614 / 241.6786 / 216.75
Total / 12 / 235.8225 / 18.55096 / 5.35520 / 224.0358 / 247.6092 / 216.74
LSD; Multiple Comparisons of LDH results
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

„H”

GP / L1 / -3.45000 / 8.52896 / .696 / -23.1178 / 16.2178
L5 / -20.81667* / 8.52896 / .041 / -40.4845 / -1.1489
L8 / 24.64667* / 8.52896 / .020 / 4.9789 / 44.3145

„H+C”

GP / L1 / -16.78333* / 6.11374 / .025 / -30.8817 / -2.6850
L5 / -21.72000* / 6.11374 / .007 / -35.8183 / -7.6217
L8 / 25.81667* / 6.11374 / .003 / 11.7183 / 39.9150

Shoot

GP / L1 / -40.22000* / 5.02835 / .000 / -51.8154 / -28.6246
L5 / -12.25333* / 5.02835 / .041 / -23.8487 / -.6579
L8 / 2.86333 / 5.02835 / .585 / -8.7321 / 14.4587

* The mean difference is significant at the 0.05 level.

ASAT; Descriptives
N / Mean / Std. Deviation / Std. Error / 95% Confidence Interval for Mean / Minimum
Lower Bound / Upper Bound
H / L1 / 3 / 32.4433 / 4.29132 / 2.47760 / 21.7831 / 43.1036 / 28.42
L5 / 3 / 48.7733 / 3.33506 / 1.92550 / 40.4886 / 57.0581 / 45.45
L8 / 3 / 28.8953 / 4.13676 / 2.38836 / 18.6190 / 39.1716 / 24.13
GP / 3 / 17.5700 / 4.73076 / 2.73131 / 5.8181 / 29.3219 / 12.13
Total / 12 / 31.9205 / 12.19613 / 3.52072 / 24.1714 / 39.6696 / 12.13
H+C / L1 / 3 / 38.4733 / 7.09272 / 4.09498 / 20.8540 / 56.0926 / 31.27
L5 / 3 / 14.5733 / 2.12420 / 1.22641 / 9.2965 / 19.8501 / 12.89
L8 / 3 / 24.0767 / 1.24557 / .71913 / 20.9825 / 27.1708 / 23.02
GP / 3 / 8.9967 / .74440 / .42978 / 7.1475 / 10.8459 / 8.21
Total / 12 / 21.5300 / 12.10174 / 3.49347 / 13.8409 / 29.2191 / 8.21
Shoot / L1 / 3 / 61.3000 / 6.99548 / 4.03884 / 43.9223 / 78.6777 / 56.96
L5 / 3 / 62.1067 / 10.87532 / 6.27887 / 35.0909 / 89.1225 / 54.76
L8 / 3 / 68.0433 / 11.47195 / 6.62333 / 39.5454 / 96.5412 / 61.42
GP / 3 / 48.9918 / 6.15720 / 3.55486 / 33.6965 / 64.2871 / 44.85
Total / 12 / 60.1104 / 10.65712 / 3.07645 / 53.3392 / 66.8817 / 44.85
LSD; Multiple Comparisons of ASAT results
(I) line / (J) line / Mean Difference
(I-J) / Std. Error / Sig. / 95% Confidence Interval
Lower Bound / Upper Bound

„H”

GP / L1 / -14.87333* / 3.39193 / .002 / -22.6951 / -7.0515
L5 / -31.20333* / 3.39193 / .000 / -39.0251 / -23.3815
L8 / -11.32533* / 3.39193 / .010 / -19.1471 / -3.5035

„H+C”

GP / L1 / -29.47667* / 3.08016 / .000 / -36.5795 / -22.3738
L5 / -5.57667 / 3.08016 / .108 / -12.6795 / 1.5262
L8 / -15.08000* / 3.08016 / .001 / -22.1829 / -7.9771

Shoot

GP / L1 / -12.30820 / 7.49139 / .139 / -29.5834 / 4.9670
L5 / -13.11487 / 7.49139 / .118 / -30.3901 / 4.1603
L8 / -19.05153* / 7.49139 / .035 / -36.3267 / -1.7763

* The mean difference is significant at the 0.05 level.