Supplemental Table 1 Proteins identified from soybean xylem sap after 2D-PAGE separation and peptide mass fingerprinting (MALD-TOF MS)
Spot# / Protein ID / MOWSE / Peptides
Matched/
Searched / %
Cov. / pI
E/T / Mr
E/T / MS-Fit (50 ppm)
DB: Acc. # / NCBI BLAST
Acc. # / Signal
Sequence
1 / Beta-amylase (G. max) / 1160 / 6/17 / 14 / 5.5/5.4 / 50,000/
56,143 / SwissProt:
P10538 / No
2 / Beta-amylase (G. max) / 806590 / 13/28 / 28 / 5.5/5.4 / 50,000/
56,143 / SwissProt:
P10538 / No
5 / 3-phosphoshikimate 1-carboxyvinyltransferase (G. max) / 2.06e+6 / 12/44 / 41 / 5.3/5.1 / 44,000/
47,615 / UniProtKB: Q71LY8_SOYBN / No
6 / 3-phosphoshikimate 1-carboxyvinyltransferase (G. max) / 159252 / 11/52 / 37 / 5.3/5.1 / 44,000/
47,615 / UniProtKB: Q71LY8_SOYBN / No
7 / Putative uncharacterized protein (G. max); [homology to SGNH plant lipase-like; hydrolase superfamily] / 1.32e+6 / 6/39 / 23 / 4.9/4.9 / 42,000/
42,455 / UniProtKB: C6T9R1_SOYBN / [NP_174260.1
A. thaliana] / Yes
8 / Putative uncharacterized protein (G. max); [homology to SGNH plant lipase-like; hydrolase superfamily] / 254384 / 5/35 / 19 / 4.9/4.9 / 42,000/
42,455 / UniProtKB: C6T9R1_SOYBN / [NP_174260.1
A. thaliana] / Yes
9 / Gamma-glutamyl hydrolase (G. max) / 2513 / 6/26 / 24 / 6.6/6.1 / 35,000/
37,677 / SwissProt:
P93164 / Yes
10 / Gamma-glutamyl hydrolase (G. max) / 2530 / 6/46 / 24 / 6.9/6.1 / 35,000/
37,677 / SwissProt:
P93164 / Yes
11 / Gamma-glutamyl hydrolase (G. max) / 11577 / 7/39 / 27 / 8.1/6.1 / 35,000/
37,677 / SwissProt:
P93164 / Yes
12 / Putative uncharacterized protein (G. max); [homology to GDP-D-mannose pyrophosporylase] / 18723 / 4/29 / 22 / 9.0/7.0 / 35,000/
39,693 / UniProtKB: C6TH97_SOYBN / [ACX37458.1
G. max] / Yes
13 / Gamma-glutamyl hydrolase (G. max) / 47 / 3/8 / 14 / 9.0/6.1 / 35,000/
37,677 / SwissProt:
P93164 / Yes
14 / Unknown protein (G. max); [homology to PRX_cys, peroxiredoxin] / 330 / 2/8 / 9 / 7.0/6.4 / 32,000/
24,420 / NCBInr:
255637491 / [Q6E2Z6.1
M. truncatula] / No
15 / Unknown protein (G. max); [homology to caspase] / 5491 / 3/65 / 11 / 8.3/6.3 / 32,000/
32,139 / NCBInr:
255635503 / [XP_002525856.1
R. communis] / Yes
16 / Putative uncharacterized protein (G.max); [homology to myosin XI-K] / 2410 / 4/29 / 21 / 9.2/9.7 / 32,000/
35,741 / UnitProtKB:
C6TGN5_SOYBN / [ABJ53199.1
N. benthamiana] / No
17 / Putative uncharacterized protein (G. max); [homology to thiamin pyrophosphokinase] / 30961 / 4/39 / 7 / 9.2/5.8 / 31,000/
28,631 / UnitProtKB: C6TER0_SOYBN / [XP_002523055.1
R. communis] / No
18 / Putative uncharacterized protein (G. max); [homology to thiamin pyrophosphokinase] / 30961 / 4/66 / 23 / 9.3/5.8 / 31,000/
28,631 / UnitProtKB: C6TER0_SOYBN / [XP_002523055.1
R. communis] / No
19 / Putative uncharacterized protein (G. max); [homology to thiamin pyrophosphokinase] / 5093 / 3/24 / 23 / 9.3/5.8 / 31,000/
28,631 / UnitProtKB: C6TER0_SOYBN / [XP_002523055.1
R. communis] / No
20 / Stem 31 kDa glycoprotein (G. max) / 6.62e7 / 9/40 / 39 / 6.1/6.7 / 31,000/
29,281 / UniProtKB: P10743_SOYBN / Yes
21 / Stem 31 kDa glycoprotein (G. max) / 3.13e11 / 14/68 / 46 / 6.3/6.7 / 31,000/
29,281 / UniProtKB: P10743_SOYBN / Yes
22 / Stem 31 kDa glycoprotein (G. max) / 9.38e6 / 7/26 / 34 / 6.5/6.7 / 31,000/
29,281 / UniProtKB: P10743_SOYBN / Yes
23 / Stem 28 kDa glycoprotein (G. max) / 2.62e6 / 7/16 / 32 / 7.5/8.7 / 29,000/
29,065 / UniProtKB: P15490_SOYBN / Yes
24 / Stem 28 kDa glycoprotein (G. max) / 5456 / 5/14 / 24 / 8.0/8.7 / 29,000/
29,065 / UniProtKB: P15490_SOYBN / Yes
25 / Stem 28 kDa glycoprotein (G. max) / 317774 / 7/24 / 34 / 8.7/8.7 / 29,000/
29,065 / UniProtKB: P15490_SOYBN / Yes
26 / Stem 28 kDa glycoprotein (G. max) / 1.00e9 / 10/41 / 46 / 9.1/8.7 / 29,000/
29,065 / UniProtKB: P15490_SOYBN / Yes
27 / Putative uncharacterized protein (G.max); [homology to Kunitz-type trypsin inhibitor] / 533981 / 5/41 / 32 / 4.3/4.8 / 24,000/
23,453 / UniProtKB: C6T0C7_SOYBN / [P01070.2
G. max] / Yes
28 / Putative uncharacterized protein (G.max); [homology to Kunitz-type trypsin inhibitor, precursor] / 3.00e8 / 8/36 / 43 / 4.9/5.1 / 22,000/
21,776 / UniProtKB: C6SVL0_SOYBN / [P25272.1
G. max] / Yes
29 / Putative uncharacterized protein (G.max); [homology to Kunitz-type trypsin inhibitor, precursor] / 3.45e7 / 7/31 / 43 / 4.9/5.1 / 22,000/
21,776 / UniProtKB: C6SVL0_SOYBN / [P25272.1
G. max] / Yes
30 / Putative uncharacterized protein (G. max)[homology to thiamin pyrophosphokinase, / 837 / 2/23 / 17 / 5.8/5.8 / 17,000/
28,631 / UnitProtKB: C6TER0_SOYBN / [XP_002523055.1
R. communis] / No
31 / Putative uncharacterized protein (G. max); [homology to type II chlorophyll a/b binding protein, photosystem I] / 1664 / 2/9 / 13 / 6.5/5.9 / 17,000/
17,168 / UnitProtKB: C6TC61_SOYBN / [CAA57492.1
P. sativum] / No
32 / Unknown protein (G. max); [homology to LRR- receptor-like protein kinase] / 1816 / 2/8 / 28 / 4.4/4.7 / 33,000/
15,898 / NCBInr:
255631790 / [ACM89546.1
G. max] / No
33 / Putative uncharacterized protein (G. max); [homology to nucleosome assembly-related protein] / 654 / 2/19 / 14 / 4.5/4.2 / 32,000/
29,489 / UniProtKB:
C6T925 / [CBE70787.1
M. truncatula] / No
35 / Gamma-glutamyl hydrolase (G. max) / 3879 / 4/19 / 19 / 7.8/6.1 / 35,000/
37,677 / SwissProt:
P93164 / No
40 / Putative uncharacterized protein (G. max); [homology to F-box family protein] / 816 / 2/12 / 8 / 8.4/8.6 / 30,000/
29,308 / UniProtKB:
C6TCL9 / [XP_002312753.1
P. trichocarpa] / No
41 / Putative uncharacterized protein (G. max); [DUF1997 superfamily related] / 2121 / 3/22 / 15 / 9.3/9.1 / 30,000/
27,415 / UniProtKB:
C6TBQ5 / No
43 / Stem 31 kDa glycoprotein (G. max) / 6.62e7 / 9/42 / 39 / 6.1/6.7 / 31,000/
29,281 / UniProtKB: P10743_SOYBN / Yes
44 / Stem 31 kDa glycoprotein (G. max) / 6.45e5 / 9/23 / 24 / 5.9/6.7 / 31,000/
29,281 / UniProtKB: P10743_SOYBN / Yes
45 / Stem 28 kDa glycoprotein (G. max) / 2.62e6 / 7/16 / 32 / 7.5/8.7 / 29,000/
29,065 / UniProtKB: P15490_SOYBN / Yes
Spot identification #’s correspond to those protein spots labeled in Figure 4. Isoelectric point (pI) and molecular weight (Mr) values are given as experimental/theoretical values. MOWSE scores represent those searches performed via Protein Prospector using a peptide mass tolerance of ≤50 ppm. Peptides matched, % coverage and accession numbers within each respective database are given. Searches were confined to Glycine max database unless no clear match could be found. Putative uncharacterized proteins from the soybean database were blasted against the NCBI all-plant database and the resulting homologous proteins and accession numbers are given in brackets. Databases are as follows: National Center for Biotechnological Information (NCBI non-redundant), Universal Protein Knowledgebase (UniProtKB) and Swiss-ProtProtein Knowledgebase (SwissProt).