Spherical baculovirosis (Penaeusmonodon-type baculovirus)
OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Spherical baculovirosis(Penaeusmonodon-type baculovirus)
Address of laboratory: / Aquaculture Pathology Laboratory
Department of Veterinary Science and Microbiology
University of Arizona
Building 90, Room 202 Pharmacy/Microbiology
Tucson, AZ 85721
UNITED STATES OF AMERICA
Tel.: / (1.520) 621.84.14
Fax: / (1.520) 621.48.99
e-mail address: /
website: /
Name (including Title and Position) of Head of Laboratory (Responsible Official): / Dr Donald V. Lightner
Professor
Name(including Title and Position) of OIE Reference Expert: / Dr Donald V. Lightner
Professor
Name (including Title and Position) of writer of this report
(if different from above): / Dr Donald V. Lightner
Linda Nunan, Assistant Staff Scientist
Part I: Summary of general activities related to the disease
1.Test(s) in use/or available for the specified disease/topic at your laboratory
Test / For / Specificity / TotalPCR /
Surveillance & SPF verification
/ All known MB viral genotypes / 335Histology
/ Initial diagnosis & surveillance / Lesions / 2419ISH
/ Confirmatory diagnosis / All known MB viral genotypes / 0Real-time PCR
/ Confirmatory diagnosis & quantification of viral load / All known MB viral genotypes / 02.Production and distribution of diagnostic reagents
Date / Country / Materialprovided / Quantity sent04/20/2011 / Madagascar / MBV primers / 2 tubes
06/11/2011 / India / MBV clone / 1 vial
11/23/2011 / SaudiArabia / MBV clone / 1 vial
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines
a)Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results
None
b)Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results
None
4.Preparation and supply of international reference standards for diagnostic tests or vaccines
See section Part I-2:
Plasmid DNA for use as positive control in PCR reactions
5.Research and development of new procedures for diagnosis and control
Duplex real-time PCR for detection and quantification of monodonbaculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeusmonodon:
Tang K.F.J. D.V. Lightner.In press.Duplex real-time PCR for detection and quantification of monodonbaculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeusmonodon.Diseases of Aquatic Organisms.
6.Collection, analysis and dissemination of epizootiological data relevant to international disease control
None
7.Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned
- Quality assurance of sample material provided is achieved by pre-testing the sample material for the pathogen of interest, by PCR, prior to shipment.
- Biosafety and biosecurity is achieved by following federal regulations for disposal of infectious materials and through the inland location of the laboratory, as no marine pathogens would be able to survive in the natural environment of the Sonora Desert.
8.Provision of consultant expertise to OIE or to OIE Member Countries
Chapters for the OIE Aquatic Animal Health Code – assisted in preparation
Lightner DV. 2011. Aquatic Animal Health Code, 14th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris:
- Infectious hypodermal and haematopoietic necrosis, Chapter 9.2.
- Infectious myonecrosis, Chapter 9.3.
- Necrotizing Hepatopancreatitis, Chapter 9.4.
- Taura syndrome, Chapter 9.5.
- White spot disease, Chapter 9.6.
- White tail disease, Chapter 9.7.
- Yellow head disease, Chapter 9.8.
Chapters for the OIE Manual of Diagnostic Tests for Aquatic Animals
Lightner DV.In press (2012).Diagnostic Manual for Aquatic Animal Diseases, 7th Edition. World Organization for Animal Health (Office International des Epizooties = OIE), Paris:
- Disinfection of Crustacean Farms, Section C of Methods for Disinfection of Aquaculture Establishments.
- Diseases of Crustaceans, General Information.
- Infectious Hypodermal and Hematopoietic Necrosis.
- Infectious Myonecrosis.
- Taura Syndrome.
- Tetrahedral Baculoviruosis (Baculoviruspenaei).
- Spherical Baculovirosis (Penaeusmonodon-type baculovirus).
Advisor to the Aquatic Animal Health Standards Commission, Office International Des Epizooties, Paris.Term 2009-2011, Advisor (~10 days/year). I was appointed to a third 3-year term by the OIE Central Bureau, beginning in May 2009, on the OIE Aquatic Animal Health Standards Commission (AAHSC) which meets 2 times per year to consider new diagnostic methods for OIE listed and notifiable aquatic animal diseases; to consider changes to the OIE Aquatic Animal Code; and to make recommendations to the Director General and to the OIE World Assembly of Member Country Delegates. In 2011, I participated in the AAHSC meeting of October 3-7 at the OIE Headquarters in Paris.
9.Provision of scientific and technical training to personnel from other OIE Member Countries
• Hosted Borlaug scholar MrBambangHanggono, of the Indonesian Government’s Situbondo Laboratory
MrHanggono visited the Aquaculture Pathology Laboratory for 12 weeks (17 September to 3 December, 2011). MrHanggono’s training emphasized histopathology and molecular diagnostic tests for major shrimp diseasesincluding Tetrahedralbaculovirosis virus.
10.Provision of diagnostic testing facilities to other OIE Member Countries
None.
11.Organisation of international scientific meetings on behalf of OIE or other international bodies
None.
12.Participation in international scientific collaborative studies
None
13.Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)
Presentations at international conferences and meetings
Invited speaker OECD Symposium, Halifax, Canada. Given by OECD Co-operative Research Program, Paris, 8/7/2011.
Invited keynote speaker, Honduras. Given by V SimposioNicovita, Honduras, 11/24/2011.(100%).
Invited keynote speaker, Ecuador. Given by XIII CongresoEcuatoriano de Acuicultura and Aquaexpo, 10/20/2011.
Invited speaker - OIE Global Conference on Aqiuatic Animal Health, Panama. Given by OIE , 6/29/2011.
Invited keynote speaker, Panama City, Panama. Givenby El Comite Organizador del X Simposio International de Camaron de Cultivo y Exhibicion Comercial, 5/13/2011.
Scientific publications in peer-reviewed journals
Lightner D.V. and R.M. Redman. 2010. The global status of significant infectious diseases of farmed shrimp. Asian Fisheries Society Journal 23(4): 383-426.
Lightner D.V. 2011. Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review. Journal of Invertebrate Pathology 106: 110-130.
Lightner, D.V. 2011.Status of shrimp diseases and advances in shrimp health management.pp. 121-134, in M.G. Bondad-Reantaso, J.B. Jones, F. Corsin and T. Aoki (editors), Diseases in Asian Aquaculture VII.Fish Health Section, Asian Fisheries Society, Selangor, Malaysia.385 p.
Lightner, D.V. and R.M. Redman."In press".Development of specific pathogen-free (SPF) shrimp stocks and their application to sustainable shrimp farming.Woodhead Publishing, LTD., Cambridge. UK.
Tang K.F.J., and D.V. Lightner.In press.Duplex real-time PCR for detection and quantification of monodonbaculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeusmonodon.Diseases of Aquatic Organisms.
Other communications
Lightner, D.V. 2011. Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. . Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. Panorama Acuícola 16(4): 8-20.
Arce, S.M., S.M. Moss and D.V. Lightner. 2011. Biosecurity principles for sustainable production using SPF shrimp. Global Aquaculture Advocate. May/June 2011, 14-16.
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Annual reports of OIE Reference Centres, 20111